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General Information
Symbol
Dmel\zip
Species
D. melanogaster
Name
zipper
Annotation Symbol
CG15792
Feature Type
FlyBase ID
FBgn0265434
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
myosin II, MyoII, myosin heavy chain, MHC, nonmuscle myosin II
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:24,990,570..25,011,965 [-]
Recombination map
2-107
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. (Q99323)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
Predictions / Assertions
Summaries
Gene Group (FlyBase)
MYOSINS -
Myosins are motor proteins which use the hydrolysis of ATP to drive movement along actin filaments. Myosins can be divided into two groups. Conventional myosins (myosin II) are involved in generating the mechanical force for muscle contraction. Unconventional myosins are involved in processes such as cell motility, cytokinesis and trafficking. (Adapted from FBrf0134714).
Protein Function (UniProtKB)
Nonmuscle myosin appears to be responsible for cellularization. Required for morphogenesis and cytokinesis (PubMed:24786584). Necessary for auditory transduction: plays a role in Johnston's organ organization by acting in scolopidial apical attachment (PubMed:27331610). Interaction with the myosin ck may be important for this function (PubMed:27331610).
(UniProt, Q99323)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Mhc-c: Myosin heavy chain-cytoplasmic (D.P. Kiehart)
Encodes a 205 kilodalton myosin heavy chain found in Drosophila cell lines and all Drosophila developmental stages. Antibodies raised against this protein crossreact, but weakly with muscle myosin heavy chain. First appears in preblastoderm embryos; diffusely distributed until syncytial blastoderm at which time localization to cortex and pole cells observed; at cleavage furrow, canals at the time of cellularization; transiently present at points of invagination during gastrulation (Young et al., 1987, 1991).
zip: zipper
The wild-type allele of zipper is expressed in the nervous system during development and was believed to encode an integral membrane protein necessary for normal axon patterning (Zhao et al., 1988); however it has more recently been shown to encode the heavy chain of cytoplasmic myosin (Kierhart). The mutants are embryonic lethals; abnormalities include a small hole in the ventral thorax, distortion of ventral denticle rows, and defects in head involution and dorsal closure (Nusslein-Volhard et al., 1984; Cote et al., 1987). These defects vary in different alleles and in different embryos from the same egg laying (Cote et al., 1987). The nervous system of mutant embryos also differentiates abnormally, showing local defects in the fasciculation pattern of axons (Zhao et al., 1988), as indicated by antibody stains for neurons and their axons. These CNS abnormalities can be detected after germ band shortening and, together with the molecular data, suggested that neurological rather than epidermal defects are the primary ones in zip mutants (Cote et al., 1987).
Summary (Interactive Fly)
Myosin II or non-muscle myosin - motor protein - crucial functions in motility, cytokinesis, dorsal closure and cytoplasmic transport - myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue
Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
7

Please see the GBrowse view of Dmel\zip or the JBrowse view of Dmel\zip for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.52
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0072399
6482
2056
FBtr0072398
6796
2011
FBtr0100466
6298
1971
FBtr0100467
6362
2016
FBtr0302572
6325
1971
FBtr0302573
6322
1979
FBtr0302574
6319
1971
FBtr0302575
6456
1964
FBtr0306576
6386
2024
Additional Transcript Data and Comments
Reported size (kB)
6.455, 6.391, 6.335, 6.271 (sequence analysis)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0072306
236.6
2056
5.30
FBpp0072305
231.5
2011
5.15
FBpp0099894
226.9
1971
5.13
FBpp0099896
232.1
2016
5.27
FBpp0291728
226.9
1971
5.13
FBpp0291729
227.8
1979
5.13
FBpp0291730
226.9
1971
5.13
FBpp0291731
226.8
1964
5.20
FBpp0297531
233.0
2024
5.27
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1971 aa isoforms: zip-PC, zip-PE, zip-PG
Additional Polypeptide Data and Comments
Reported size (kDa)
2057, 2017, 2012, 1972 (aa)
2017, 1972 (aa); 227 (kD predicted)
500 (aa); 56 (kD predicted)
Comments
zip protein is most similar to metazoan smooth and nonmuscle myosins.
A 2017aa zip protein is predicted by translation from the first AUG of the "long" zip transcript. Antibodies directed against a 15aa peptide specific for part of the amino-terminal 45aa extension react with a protein of the expected size providing evidence for its existence in vivo.
External Data
Subunit Structure (UniProtKB)
Interacts with sau (PubMed:24786584). Interacts with ck and Ubr3 (PubMed:27331610).
(UniProt, Q99323)
Post Translational Modification
Ubiquitinated.
(UniProt, Q99323)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\zip using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (49 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:sqh; FB:FBgn0003514
inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR008989
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR001609, InterPro:IPR004009
(assigned by InterPro )
Biological Process (32 terms)
Terms Based on Experimental Evidence (29 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:l(2)gl; FB:FBgn0002121
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
Cellular Component (13 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
colocalizes_with apical cortex
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from mutant phenotype
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
colocalizes_with contractile ring
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from physical interaction with FLYBASE:sqh; FB:FBgn0003514
inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
traceable author statement
non-traceable author statement
inferred from sequence or structural similarity
non-traceable author statement
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
RNase protection, primer extension, SI map
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
zip expression is strong in the amnioserosa and weak in the dorsal epidermis at embryonic stage 11,12 and gone from the amnioserosa and strong in the dorsal epidermis at stage 13.
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with apical cortex
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from mutant phenotype
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
colocalizes_with contractile ring
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from physical interaction with FLYBASE:sqh; FB:FBgn0003514
inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\zip in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 34 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 25 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of zip
Transgenic constructs containing regulatory region of zip
Deletions and Duplications ( 7 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
elongation stage spermatid & nucleus
filamentous actin & denticle field primordium
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (31)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
12 of 15
No
Yes
 
11 of 15
No
Yes
10 of 15
No
Yes
 
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
 
4 of 15
No
No
4 of 15
No
No
 
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (28)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
9 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (28)
10 of 13
Yes
Yes
9 of 13
No
Yes
9 of 13
No
Yes
8 of 13
No
Yes
7 of 13
No
Yes
4 of 13
No
No
4 of 13
No
No
4 of 13
No
No
4 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
2 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (24)
7 of 12
Yes
Yes
5 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (47)
14 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
Yes
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (15)
15 of 15
Yes
Yes
8 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (20)
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (5)
12 of 15
Yes
Yes
3 of 15
No
No
3 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (5)
10 of 12
Yes
No
8 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
1 of 12
No
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900CI )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500CC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00CM )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00BY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G009J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (8)
7 of 10
3 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 4 )
Potential Models Based on Orthology ( 4 )
Modifiers Based on Experimental Evidence ( 4 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
suppressible
External Data
Subunit Structure (UniProtKB)
Interacts with sau (PubMed:24786584). Interacts with ck and Ubr3 (PubMed:27331610).
(UniProt, Q99323 )
Linkouts
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-107
Cytogenetic map
Sequence location
2R:24,990,570..25,011,965 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
60E11-60E12
Limits computationally determined from genome sequence between P{EP}CG2790EP412&P{EP}CG3776EP835 and P{EP}zipEP856&P{PZ}l(2)1048103263
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
60F1-60F3
(determined by in situ hybridisation)
60E-60F
(determined by in situ hybridisation)
60E9-60F1
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (28)
Genomic Clones (17)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (157)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of
Source for database merge of
Source for merge of: zip anon-WO0140519.37
Source for merge of: zip l(2)17F1
Additional comments
Source for merge of zip anon-WO0140519.37 was sequence comparison ( date:051113 ).
Other Comments
Host gene for maternally inherited stable intronic sequence RNA (sisRNA).
Candidate stable intronic sequence RNA (sisRNA) identified within 5'UTR of this gene.
Overexpression of zip in D.melanogaster males results in paternal-effect lethality that mimics the fertilisation defects associated with cytoplasmic incompatibility (CI) caused by Wolbachia infection.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
zip is necessary for normal morphology and behaviour of the leading edge cells during embryonic dorsal closure.
zip is required for myofibril formation. zip may function at the muscle termini and the Z line as an actin crosslinker and may act to maintain the structural integrity of the sarcomere.
The zip protein inhibits actomyosin dependent basal protein targeting in neuroblasts.
158 deficiency chromosomes have been screened for second site non-complementation with zip alleles, identifying at least 39 SSNC loci, including Tm1, vkg and Rho1.
Mutation rate at microsatellite loci in 119 lines maintained for approximately 250 generations is estimated to be 6.3x10-6, at least one order of magnitude lower than the mutation rate in mammals.
One of a class of genes with TATA-less promoters that have the conserved DPE sequence.
zip is required for cell sheet movements in embryos. The zip gene product is responsible for a surprisingly diverse array of cell shape changes throughout development.
A serine kinase is tightly associated with l(2)gl. Activation of the serine kinase results in the disassociation of zip from the l(2)gl complex without affecting the homo-oligomerisation of l(2)gl.
Organisation of the transcription unit and alternative splicing of zip are analysed.
The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to study the zygotic lethal mutation.
The p127 protein of the l(2)gl gene is a component of a cytoskeletal network when complexed with a nonmuscle myosin II heavy chain protein, zip.
Severe alleles disrupt cell shape changes required for embryogenesis.
The zip gene product, nonmuscle myosin, is required for generating and/or maintaining the cell shapes that change during the course of morphogenesis and provides a link between myosin and morphogenesis.
Analysis of myosin heavy chain antibody staining in Drosophila tissue culture cells and premyogenic embryos suggests that zip rather than Mhc is associated with the nuclear envelope.
The interaction of zip and br depends on loss of br function and is temperature-dependent. Flies reared at 18oC show a higher penetrance of the mlf phenotype (malformed syndrome), wing malformations and leg defects, than those reared at 25oC.
zip mutant embryos display defects in dorsal closure, head involution, segmentation and neural pathfinding.
The distribution of zip protein during embryogenesis has been analysed.
Fas3, mys, disco, zip, l(2)gl, N and Egfr mutants show an additive phenotype in combination with Fas1TE89Da.
An alternatively spliced exon at the 5' end of zip generates two distinct transcripts. The coding region reveals extensive homology with other conventional myosins.
The zip region is defined by the proximal breakpoint of Df(2R)Kr10 (map position 0 to +3.5) and by the proximal breakpoint of Df(2R)gsb (map position -55 to -49).
Included in genetic and molecular analysis of the zipper-gooseberry region.
Five structural and three functional criteria demonstrate a protein purified from S2, S3 and Kc culture cells is a cytoplasmic myosin.
The gene sequenced by Zhao et al. (Zhao et al., 1988, EMBO J. 7) as zip is not zip but the adjacent gene, uzip.
The mutants are embryonic lethals; abnormalities include a small hole in the ventral thorax, distortion of ventral denticle rows and defects in head involution and dorsal closure (FBrf0041708; FBrf0046110). These defects vary in different alleles and in different embryos from the same egg laying (FBrf0046110).
Encodes a 205 kilodalton myosin heavy chain found in Drosophila cell lines and all Drosophila developmental stages. Antibodies raised against this protein crossreact, but weakly with muscle myosin heavy chain. First appears in preblastoderm embryos; diffusely distributed until syncytial blastoderm at which time localization to cortex and pole cells observed; at cleavage furrow, canals at the time of cellularization; transiently present at points of invagination during gastrulation (FBrf0046628; FBrf0053751). produces a truncated myosin heavy chain on Western blots and fails to complement zip1 and zip2. Western blots also indicate zip2 fails to accumulate myosin heavy chain.
Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 80 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Linkouts
DroID - A comprehensive database of gene and protein interactions.
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (73)
Reported As
Symbol Synonym
Dronm-MII
Mhc-c1
anon-WO0140519.37
l(2)17F1
zip
(Chang et al., 2019, Finegan et al., 2019, Proag et al., 2019, Córdoba and Estella, 2018, Lebreton et al., 2018, Lee et al., 2018, Mortensen et al., 2018, Park et al., 2018, Tsai et al., 2018, Bulgakova et al., 2017, Fochler et al., 2017, Hermle et al., 2017, Ly et al., 2017, Rothenbusch-Fender et al., 2017, Simões et al., 2017, Sun et al., 2017, Tay and Pek, 2017, Transgenic RNAi Project members, 2017-, Fear et al., 2016, Gene Disruption Project members, 2016-, Ng et al., 2016, Rousso et al., 2016, Weng and Wieschaus, 2016, Davis et al., 2015, Gene Disruption Project members, 2015-, Guglielmi et al., 2015, Heissler et al., 2015, Kim et al., 2015, Levayer et al., 2015, Monier et al., 2015, Pek et al., 2015, Sato et al., 2015, Winkler et al., 2015, Ashwal-Fluss et al., 2014, Guo et al., 2014, He et al., 2014, Kitazawa et al., 2014, Merlo et al., 2014, Mukherjee et al., 2014, Nie et al., 2014, Rauskolb et al., 2014, Rudrapatna et al., 2014, Aldaz et al., 2013, Beckett et al., 2013, Bonn et al., 2013, Guthrie, 2013, Külshammer and Uhlirova, 2013, Kwon et al., 2013, Lee and Harris, 2013, Moon and Matsuzaki, 2013, Shen et al., 2013, Taliaferro et al., 2013, Thomae et al., 2013, Abreu-Blanco et al., 2012, Duboff et al., 2012, Gault et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Lesch and Page, 2012, Murray et al., 2012, Rodriguez et al., 2012, Sano et al., 2012, Weber et al., 2012, Axelsson et al., 2011, Escudero et al., 2011, Farkaš et al., 2011, Friedman et al., 2011, Guest et al., 2011, Jiang et al., 2011, Kim et al., 2011, Monier et al., 2011, Neumüller et al., 2011, Rees et al., 2011, Seabrooke and Stewart, 2011, Sun et al., 2011, Baek et al., 2010, Campos et al., 2010, David et al., 2010, Djiane and Mlodzik, 2010, Franke et al., 2010, Gettings et al., 2010, Knowles-Barley et al., 2010, Kwon et al., 2010, Lowery et al., 2010, Monier et al., 2010, Neubueser and Hipfner, 2010, Okumura et al., 2010, Rui et al., 2010, Saja et al., 2010, Simões et al., 2010, Simone and DiNardo, 2010, Aerts et al., 2009, Bertet et al., 2009, Chung et al., 2009, Dworkin et al., 2009, Landsberg et al., 2009, Martin et al., 2009, Patch et al., 2009, Warner and Longmore, 2009, Chen et al., 2008, Christensen et al., 2008.9.3, Fiehler and Wolff, 2008, Homem and Peifer, 2008, Okumura et al., 2008, Pope and Harris, 2008, Rodriguez-Diaz et al., 2008, Todi et al., 2008, Zahedi et al., 2008, Boettner and Van Aelst, 2007, Buszczak et al., 2007, Chung et al., 2007, Dietzl et al., 2007, Dilks and DiNardo, 2007, Escudero et al., 2007, Farkas et al., 2007, Fiehler and Wolff, 2007, Franke et al., 2007, Kaltenbach et al., 2007, Kirchner et al., 2007, Kirchner et al., 2007, Stuart et al., 2007, Brodu and Casanova, 2006, Franke et al., 2006, Hammonds and Fristrom, 2006, Hawley and Gilliland, 2006, Hickson et al., 2006, Verdier et al., 2006, Gim et al., 2001, Winter et al., 2001)
Name Synonyms
Myo-II heavy chain
MyoII heavy chain
Myosin heavy chain-cytoplasmic
Zipper heavy chain
cytoplasmic myosin
cytoplasmic myosin heavy chain
cytoplasmic myosin-II
lethal (2) 17F1
myo-II heavy chain
myosin-heavy-chain-cytoplasmic
myosins II
non muscle myosin
non-muscle Myosin II
non-muscle myosin
non-muscle myosin heavy-chain
non-muscle myosin-II
nonmuscle Myosin II heavychain
nonmuscle myosin II heavy chain
nonmuscle myosin heavy chain
Secondary FlyBase IDs
  • FBgn0005634
  • FBgn0002742
  • FBgn0004415
  • FBgn0010524
  • FBgn0044594
  • FBgn0026658
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Study focus (0)
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References (572)