myosin II, MyoII, MHC, myosin heavy chain, Myo
Myosin II or non-muscle myosin - motor protein - crucial functions in motility, cytokinesis, dorsal closure and cytoplasmic transport - myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.52
6.455, 6.391, 6.335, 6.271 (sequence analysis)
A 2017aa zip protein is predicted by translation from the first AUG of the "long" zip transcript. Antibodies directed against a 15aa peptide specific for part of the amino-terminal 45aa extension react with a protein of the expected size providing evidence for its existence in vivo.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\zip using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\zip in GBrowse 2
Maps to 2R.
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Host gene for maternally inherited stable intronic sequence RNA (sisRNA).
Candidate stable intronic sequence RNA (sisRNA) identified within 5'UTR of this gene.
Overexpression of zip in D.melanogaster males results in paternal-effect lethality that mimics the fertilisation defects associated with cytoplasmic incompatibility (CI) caused by Wolbachia infection.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
zip is necessary for normal morphology and behaviour of the leading edge cells during embryonic dorsal closure.
The zip protein inhibits actomyosin dependent basal protein targeting in neuroblasts.
Mutation rate at microsatellite loci in 119 lines maintained for approximately 250 generations is estimated to be 6.3x10-6, at least one order of magnitude lower than the mutation rate in mammals.
One of a class of genes with TATA-less promoters that have the conserved DPE sequence.
Organisation of the transcription unit and alternative splicing of zip are analysed.
Severe alleles disrupt cell shape changes required for embryogenesis.
The zip gene product, nonmuscle myosin, is required for generating and/or maintaining the cell shapes that change during the course of morphogenesis and provides a link between myosin and morphogenesis.
The interaction of zip and br depends on loss of br function and is temperature-dependent. Flies reared at 18oC show a higher penetrance of the mlf phenotype (malformed syndrome), wing malformations and leg defects, than those reared at 25oC.
zip mutant embryos display defects in dorsal closure, head involution, segmentation and neural pathfinding.
An alternatively spliced exon at the 5' end of zip generates two distinct transcripts. The coding region reveals extensive homology with other conventional myosins.
Included in genetic and molecular analysis of the zipper-gooseberry region.
Five structural and three functional criteria demonstrate a protein purified from S2, S3 and Kc culture cells is a cytoplasmic myosin.
The mutants are embryonic lethals; abnormalities include a small hole in the ventral thorax, distortion of ventral denticle rows and defects in head involution and dorsal closure (FBrf0041708; FBrf0046110). These defects vary in different alleles and in different embryos from the same egg laying (FBrf0046110).
Encodes a 205 kilodalton myosin heavy chain found in Drosophila cell lines and all Drosophila developmental stages. Antibodies raised against this protein crossreact, but weakly with muscle myosin heavy chain. First appears in preblastoderm embryos; diffusely distributed until syncytial blastoderm at which time localization to cortex and pole cells observed; at cleavage furrow, canals at the time of cellularization; transiently present at points of invagination during gastrulation (FBrf0046628; FBrf0053751). produces a truncated myosin heavy chain on Western blots and fails to complement zip1 and zip2. Western blots also indicate zip2 fails to accumulate myosin heavy chain.