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General Information
Symbol
Dmel\CrebB
Species
D. melanogaster
Name
Cyclic-AMP response element binding protein B
Annotation Symbol
CG6103
Feature Type
FlyBase ID
FBgn0265784
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
dCREB2, CREB, CrebB-17A, dCREB2-b, dCREB2-a
Key Links
Genomic Location
Cytogenetic map
Sequence location
X:18,366,357..18,377,864 [+]
Recombination map
1-60
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the bZIP family. ATF subfamily. (Q9VWW0)
Molecular Function (GO)
[Detailed GO annotations]
Summaries
Gene Group (FlyBase)
BASIC LEUCINE ZIPPER TRANSCRIPTION FACTORS -
The basic leucine zipper (bZIP) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60-80 amino acid bZIP domain: a basic DNA binding domain followed by a leucine zipper dimerization domain. (Adapted from FBrf0152056).
Protein Function (UniProtKB)
Isoform E is a PKA-dependent transcriptional activator. Isoform J is a direct antagonist of activation by isoform E in cell culture. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters (PubMed:7651429, PubMed:15219829). Has a role in long-term memory (PubMed:29473541).
(UniProt, Q9VWW0)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
5

Please see the GBrowse view of Dmel\CrebB or the JBrowse view of Dmel\CrebB for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Western blot analysis of endogenous protein extracts identifies minor amounts of a short CrebB isoform that apparently results from translational initiation at the downstream start codon MQKVV... (FBrf0221434). Such minor variants are not typically represented in FlyBase gene models; FlyBase analysis.
Gene model reviewed during 5.53
Gene model reviewed during 5.56
Gene model reviewed during 6.12
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.43
Evidence for alternative splicing from FBrf0083527.
Gene model reviewed during 5.42
Gene model reviewed during 5.46
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0074557
2072
285
FBtr0074555
2198
327
FBtr0112878
3518
327
FBtr0112879
3380
281
FBtr0308232
2102
296
FBtr0310340
2138
327
FBtr0310341
2049
281
FBtr0346093
2091
281
FBtr0446106
2150
331
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0074329
30.3
285
7.02
FBpp0074327
34.4
327
6.36
FBpp0111791
34.4
327
6.36
FBpp0111792
29.8
281
6.36
FBpp0300552
30.5
296
6.64
FBpp0302023
34.4
327
6.36
FBpp0302024
29.8
281
6.36
FBpp0311927
29.8
281
6.36
FBpp0402805
34.9
331
7.02
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

327 aa isoforms: CrebB-PG, CrebB-PI, CrebB-PN
281 aa isoforms: CrebB-PJ, CrebB-PO, CrebB-PP
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
The product of the longest CrebB transcript ("dCREB-2a") acts as a PKA-dependent transcriptional activator in transient transfection assays. This activation is blocked when an alternately spliced CrebB transcript (dCREB-2b") is cotransfected with the transcriptional activator ("dCREB-2a").
The product of the longest CrebB transcript ("dCREB-2a") acts as a PKA-dependent transcriptional activator in transient transfection assays. This activation is blocked when an alternately spliced CrebB transcript (dCREB-2b") is cotransfected with the transcriptional activator ("dCREB-2a"). When the middle 2 leucines in the bZIP domain of the antagonist protein (dCREB-2b") are changed to valine, the antagonist is no longer able to block transcriptional activation.
DNA binding assayed
g430806 and g1110570 appear to be alternative splice variants. It is unclear is either is a complete sequence
External Data
Subunit Structure (UniProtKB)
Homodimer.
(UniProt, Q9VWW0)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CrebB using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (20 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from mutant phenotype
(assigned by UniProt )
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Biological Process (15 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
(assigned by UniProt )
inferred from expression pattern
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
non-traceable author statement
non-traceable author statement
traceable author statement
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001119557
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CrebB in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 19 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 38 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CrebB
Transgenic constructs containing regulatory region of CrebB
Deletions and Duplications ( 0 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
8 of 15
No
Yes
8 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
6 of 15
No
Yes
6 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
8 of 13
Yes
Yes
7 of 13
No
Yes
4 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
5 of 12
Yes
Yes
3 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (10)
9 of 15
Yes
Yes
8 of 15
No
Yes
8 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
7 of 15
Yes
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (17)
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
1 of 15
Yes
No
1 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (4)
1 of 12
Yes
Yes
1 of 12
Yes
Yes
1 of 12
Yes
No
1 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190GDL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BP8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0DOV )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0DMT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (2)
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homodimer.
    (UniProt, Q9VWW0 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-60
    Cytogenetic map
    Sequence location
    X:18,366,357..18,377,864 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    17B-17B
    Left limit from in situ hybridisation (FBrf0082623) Right limit from in situ hybridisation (FBrf0082623)
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    17A2-17A2
    17A2-17A2
    (determined by in situ hybridisation)
    17A-17A
    (determined by in situ hybridisation)
    17A-17B
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (24)
    Genomic Clones (17)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (9)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      RNAi and Array Information
      Linkouts
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: CrebB-17A CG6103
      Source for database merge of
      Source for merge of: CrebB-17A l(1)17Af
      Source for merge of: CrebB-17A CrebB-17B
      Additional comments
      Other Comments
      Expression is affected in Fmr1 mutants.
      Cycling CrebB-17A activity is abolished in a per mutant, indicating that CrebB-17A and per affect each other and may participate in the same regulatory feedback loop.
      Mutation in CrebB-17A shortens circadian locomotor rhythms and dampens the oscillations of per.
      CrebB-17A supports cycling of the per/tim oscillator.
      CrebB-17A binds to the Ubx cAMP response element (CRE), located in an enhancer, and ubiquitous expression of a dominant negative form of CrebB-17A suppresses CRE-mediated reporter gene expression and reduces lab expression in the endoderm.
      CrebB-17A acts in parallel with Fas2 to cause an increase in synaptic strength. cAMP initiates these parallel changes to achieve long-term synaptic enhancement. Expression of the CrebB-17A repressor in the dnc mutant blocks functional but not structural plasticity. Expression of the CrebB-17A activator increases synaptic strength, but only in Fas2 mutants that increase bouton number, due to increased presynaptic transmitter release. Expression of CrebB-17A in a Fas2 mutant background genetically reconstitutes cAMP-dependent plasticity. Thus, cAMP initiates parallel changes in CrebB-17A and Fas2 to achieve long-term synaptic enhancement.
      Long term memory formation can be prevented by an inducible repressor form of CrebB-17A.
      Long term memory (LTM) is blocked specifically by induced expression of a repressor isoform of CrebB-17A. LTM formation is enhanced after induced expression of an activator form of CrebB-17A. LTM formation depends on phosphorylation of the CrebB-17A product. FlyBase curator comment: see FBrf0180428 for re-examination of these conclusions in light of discovery that CrebB-17Aa.hs actually encodes a mutant form of CrebB-17A isoform a. In contrast to results reported in FBrf0080505, FBrf0180428 reports that heat shock of CrebB-17Aa.hs was found to exert no effect on LTM in 1x training session - though assay was carried out at 5, as opposed to 7 days. CrebB-17Ab.hs, however, does have an effect on LTM.
      CrebB-17A has been cloned and sequenced.
      Over-expression of the b isoform of CrebB-17A completely blocks long-term memory, but leaves anaesthesia resistant memory and learning unaffected.
      The structure of CrebB-17A is reminiscent of genes in the mammalian CREB family.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 89 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      Linkouts
      Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
      Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
      DroID - A comprehensive database of gene and protein interactions.
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (51)
      Reported As
      Symbol Synonym
      Creb B 17A
      CrebB-17B
      dCREB-2
      l(1)17Af
      Name Synonyms
      Cyclic-AMP response element binding protein B
      Cyclic-AMP response element binding protein B at 17A
      Cyclic-AMP response element binding protein B at 17B
      activator
      blocker
      cAMP response element binding
      cAMP response element-binding protein
      cAMP response-element binding protein
      cAMP-regulated enhancer-binding protein
      cAMP-response element binding protein
      cAMP-response element-binding protein
      cyclic AMP response element binding protein
      lethal(1)17Af
      Secondary FlyBase IDs
      • FBgn0014467
      • FBgn0004972
      • FBgn0014468
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (219)