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General Information
Symbol
Dmel\Doa
Species
D. melanogaster
Name
Darkener of apricot
Annotation Symbol
CG42320
Feature Type
FlyBase ID
FBgn0265998
Gene Model Status
Stock Availability
Gene Snapshot
Darkener of apricot (Doa) encodes an essential Ser/Thr protein kinase with SR proteins (RNA maturation) and the product of eEF1γ (microtubule-based organelle transport) as substrates. It contributes to somatic sex determination (splicing of dsx transcript) and development of eye, wing, leg, oocyte and bristles. [Date last reviewed: 2019-03-07]
Also Known As

Msu

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:28,888,073..28,922,772 [+]
Recombination map

3-97

RefSeq locus
NT_033777 REGION:28888073..28922772
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (22 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
Biological Process (15 terms)
Terms Based on Experimental Evidence (15 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from expression pattern
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:rl; FB:FBgn0003256
inferred from genetic interaction with FLYBASE:sev; FB:FBgn0003366
inferred from genetic interaction with FLYBASE:sina; FB:FBgn0003410
inferred from genetic interaction with FLYBASE:svp; FB:FBgn0003651
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from direct assay
inferred from mutant phenotype
inferred from high throughput mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:tra; FB:FBgn0003741
inferred from genetic interaction with FLYBASE:tra2; FB:FBgn0003742
inferred from mutant phenotype
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred by curator from GO:0000381
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. (P49762)
Summaries
Gene Group (FlyBase)
CMGC KINASES (UNCLASSIFIED) -
CMGC family members are primarily proline-directed serine/threonine kinases. (Adapted from FBrf0132098).
Pathway (FlyBase)
Positive Regulators of Toll-NF-κB Signaling Pathway -
In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).
Protein Function (UniProtKB)
Negative regulator of the copia retrotransposon element of the white (w) gene. In the eye, it is required for normal pigmentation, photoreceptor cell development and for organization of interommatidial bristles. Also essential for embryonic segmentation and differentiation of the nervous system. Functions in the control of alternative splicing by phosphorylating the arginine/serine-rich splicing factors, SR proteins.
(UniProt, P49762)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Doa: Darkener of apricot
Homozygous larval lethal, but in heterozygotes causes the copia insertion allele wa and some of its revertants to produce more pigment than usual; not only in the eye, but in the ocelli, testis sheath, and Malpighian tubules as well; enhances wsp55 resulting in less than normal pigmentation; other tested alleles not affected. Flies carrying three copies of the wild-type allele of Doa lighten wa and darken wsp55. Doa also darkens z wa in homozygous females. Heterozygotes for Doa1 or Doa2 and Doa6 or Doa7 survive rarely, and when they do, wa is darkened to nearly wild-type color; wsp55 reacts in the opposite manner, becoming nearly white; eyes of escapers have disarranged facets. Males of these heteroallelic combinations involving Doa6 are sterile despite having motile sperm; females and both sexes involving Doa7 are weakly fertile.
Gene Model and Products
Number of Transcripts
15
Number of Unique Polypeptides
11

Please see the JBrowse view of Dmel\Doa for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Merge of CG33204 and CG33553 Doa.based on FBrf0204586.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.39

Merge of CG31049 and CG33553 Doa.based on FBrf0204586.

Gene model reviewed during 6.32

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0479846
3168
599
FBtr0299742
3028
580
FBtr0299743
3693
832
FBtr0299744
4312
983
FBtr0299753
3111
580
FBtr0299745
2221
580
FBtr0299751
7311
2045
FBtr0299747
4720
1229
FBtr0299748
2904
511
FBtr0299749
2971
580
FBtr0299750
2164
580
FBtr0299752
2097
511
FBtr0308598
3967
889
FBtr0308599
5756
1535
FBtr0308600
3958
975
Additional Transcript Data and Comments
Reported size (kB)

5.5, 4.0, 3.8, 2.9, 2.7, 1.9 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0428182
70.3
599
9.24
FBpp0289020
67.9
580
10.17
FBpp0289021
95.7
832
8.86
FBpp0289022
111.3
983
9.79
FBpp0289031
68.4
580
9.33
FBpp0289023
67.9
580
10.17
FBpp0289029
226.7
2045
9.83
FBpp0289025
137.5
1229
9.40
FBpp0289026
60.2
511
9.93
FBpp0289027
67.9
580
10.17
FBpp0289028
67.9
580
10.17
FBpp0289030
60.2
511
9.93
FBpp0300822
100.6
889
8.70
FBpp0300823
170.5
1535
9.69
FBpp0300824
111.0
975
9.44
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

580 aa isoforms: Doa-PJ, Doa-PN, Doa-PT, Doa-PU
511 aa isoforms: Doa-PS, Doa-PV
Additional Polypeptide Data and Comments
Reported size (kDa)

55 (kD observed)

Comments

Doa protein possesses intrinsic protein kinase

activity when expressed in bacteria, as assayed via autophosphorylation.

Doa protein is a member of a novel highly conserved protein kinase

family called the LAMMER protein kinases. Members are characterized by an

11 aa peptide motif at kinase subdomain X, which is virtually 100%

identical in all homologs.

External Data
Post Translational Modification

Autophosphorylated on serine, threonine and tyrosine residues.

(UniProt, P49762)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Doa using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 0-24 hr AEL

Comment: reference states 0-24 hr APF

Additional Descriptive Data

The 55 kD isoform localizes to the nucleus in 0-24 hr pupal extracts.

The 105 kD isoform is restricted to the cytoplasm in 0-24 hr pupal extracts.

The 105 kD isoform is detected in all developmental stages. Equivalent amounts of the 105 kD isoform are found in male and female pupae.

Embryonic Doa protein expression patterns resemble those of Doa transcript expression, but unlike the transcript, there is no segmental variation in the localization of the protein. At stage 17, protein expression is higher in the central nervous system and the brain than in surrounding tissue. Doa protein is ubiquitous in larval imaginal discs. In the eye disc, slightly higher levels are detected posterior to the morphogenetic furrow, and higher expression is detected in the Bolwig\'s nerve.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
Reporter: P{PZ}Doa01705b
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Doa in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 68 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Doa
Transgenic constructs containing regulatory region of Doa
Deletions and Duplications ( 12 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
nurse cell & nuclear chromosome | germ-line clone
wing (with Doa7)
wing (with Doa9)
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (14)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
 
4  
8 of 15
No
Yes
8 of 15
No
Yes
1  
7 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (15)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
 
1  
8 of 15
No
Yes
8 of 15
No
Yes
8 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (12)
8 of 13
Yes
Yes
6 of 13
No
Yes
5 of 13
No
Yes
4 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (11)
8 of 12
Yes
Yes
5 of 12
No
Yes
5 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (16)
11 of 15
Yes
Yes
7 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (10)
10 of 15
Yes
Yes
3 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (3)
7 of 9
Yes
Yes
6 of 9
No
Yes
6 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
9 of 15
Yes
Yes
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
7 of 12
Yes
Yes
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900OS )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915024F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05E4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05AB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G033A )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (5)
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Dmel gene
    Ortholog showing functional complementation
    Supporting References
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    suppressible
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Positive Regulators of Toll-NF-κB Signaling Pathway -
    In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map

    3-97

    Cytogenetic map
    Sequence location
    3R:28,888,073..28,922,772 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    98F1-98F2
    Limits computationally determined from genome sequence between P{PZ}l(3)0648706487 and P{EP}EP3390EP3390
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    98F1-98F2
    98F4-98F2
    (determined by in situ hybridisation)
    98F1-98F3
    (determined by in situ hybridisation)
    98F1-98F2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Notes

    Maps approximately 1 cM from Dr.

    Stocks and Reagents
    Stocks (39)
    Genomic Clones (39)
    cDNA Clones (178)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Doa CG1658

    Source for database merge of

    Source for merge of: Doa CG33204

    Source for merge of: Doa Msu

    Source for merge of: Doa CG31049

    Additional comments

    Annotations Doa and CG33204 merged as CG42320 in release 5.9 of the genome annotation.

    Annotations CG1658 and CG31049 merged as CG33553 (which corresponds to Doa) in release 4.1 of the genome annotation.

    Other Comments

    dsRNA directed against Doa inhibits protein secretion in S2 cells, but has no apparent effect on Golgi organisation.

    dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.

    When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells is seen.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    New annotation (CG33204) in release 3 of the genome annotation.

    New annotation (CG31049) in release 3 of the genome annotation.

    No genetic interactions are seen between Doa mutants and mutations in components of the ras-MAP kinase signal transduction pathway.

    Doa mutant clones are lethal in both the germ-line and soma.

    Mutations in Doa alter sexual differentiation and interact synergistically with tra and tra2 mutations.

    Recombinant protein is shown to possess protein kinase activity and autophosphorylation with dual specificity in vitro.

    Doa locus encodes a protein kinase (FBrf0074875) and is essential for survival of retinal photoreceptors and normal development of the embryonic CNS and cuticle. Germline clonal analysis and somatic mosaics demonstrate that the Doa gene product is essential for oogenesis and that mutations are cell lethal.

    The Doa mutation suggests a connection between the regulation of specific transcriptional units such as retrotransposons and more global synapsis-dependent regulatory effects.

    The Doagene product is a protein kinase that possesses the potential for dual specificity with homologs in widely diverged eukaryotes defining a new family.

    Doa has an essential role in embryonic development and maintenance of photoreceptor cells.

    Mutations at the Doa modify the wa phenotype. Effects were determined in pairwise combinations and found to be additive or epistatic. Mutant alleles are dominant. Mutant alleles are dominant.

    Homozygous larval lethal, but in heterozygotes causes the copia insertion allele wa and some of its revertants to produce more pigment than usual; not only in the eye, but in the ocelli, testis sheath and Malpighian tubules as well; enhances wsp55 resulting in less than normal pigmentation; other tested alleles not affected. Flies carrying three copies of the wild-type allele of Doa lighten wa and darken wsp55. Doa also darkens z wa in homozygous females.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 109 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (19)
    Reported As
    Symbol Synonym
    l(3)01705
    l(3)S084402
    l(3)s2784
    Secondary FlyBase IDs
    • FBgn0024584
    • FBgn0028087
    • FBtr0085332
    • FBtr0085333
    • FBtr0085334
    • FBtr0085335
    • FBan0001658
    • FBgn0053553
    • FBgn0000480
    • FBgn0010760
    • FBgn0014864
    • FBgn0051049
    • FBgn0053204
    • FBgn0259220
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
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    References (163)