Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Merge of CG33204 and CG33553 Doa.based on FBrf0204586.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.39
Merge of CG31049 and CG33553 Doa.based on FBrf0204586.
Gene model reviewed during 6.32
5.5, 4.0, 3.8, 2.9, 2.7, 1.9 (northern blot)
Doa protein possesses intrinsic protein kinase
activity when expressed in bacteria, as assayed via autophosphorylation.
Doa protein is a member of a novel highly conserved protein kinase
family called the LAMMER protein kinases. Members are characterized by an
11 aa peptide motif at kinase subdomain X, which is virtually 100%
identical in all homologs.
Autophosphorylated on serine, threonine and tyrosine residues.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Doa using the Feature Mapper tool.
The 55 kD isoform localizes to the nucleus in 0-24 hr pupal extracts.
The 105 kD isoform is restricted to the cytoplasm in 0-24 hr pupal extracts.
The 105 kD isoform is detected in all developmental stages. Equivalent amounts of the 105 kD isoform are found in male and female pupae.
Embryonic Doa protein expression patterns resemble those of Doa transcript expression, but unlike the transcript, there is no segmental variation in the localization of the protein. At stage 17, protein expression is higher in the central nervous system and the brain than in surrounding tissue. Doa protein is ubiquitous in larval imaginal discs. In the eye disc, slightly higher levels are detected posterior to the morphogenetic furrow, and higher expression is detected in the Bolwig\'s nerve.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Doa in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Doa CG1658
dsRNA directed against Doa inhibits protein secretion in S2 cells, but has no apparent effect on Golgi organisation.
dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells is seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
New annotation (CG33204) in release 3 of the genome annotation.
New annotation (CG31049) in release 3 of the genome annotation.
No genetic interactions are seen between Doa mutants and mutations in components of the ras-MAP kinase signal transduction pathway.
Recombinant protein is shown to possess protein kinase activity and autophosphorylation with dual specificity in vitro.
Doa locus encodes a protein kinase (FBrf0074875) and is essential for survival of retinal photoreceptors and normal development of the embryonic CNS and cuticle. Germline clonal analysis and somatic mosaics demonstrate that the Doa gene product is essential for oogenesis and that mutations are cell lethal.
The Doa mutation suggests a connection between the regulation of specific transcriptional units such as retrotransposons and more global synapsis-dependent regulatory effects.
The Doagene product is a protein kinase that possesses the potential for dual specificity with homologs in widely diverged eukaryotes defining a new family.
Doa has an essential role in embryonic development and maintenance of photoreceptor cells.
Homozygous larval lethal, but in heterozygotes causes the copia insertion allele wa and some of its revertants to produce more pigment than usual; not only in the eye, but in the ocelli, testis sheath and Malpighian tubules as well; enhances wsp55 resulting in less than normal pigmentation; other tested alleles not affected. Flies carrying three copies of the wild-type allele of Doa lighten wa and darken wsp55. Doa also darkens z wa in homozygous females.