FlyBase Gene Report: Dmel\CngB

General Information

Symbol

Dmel\CngB

Species

D. melanogaster

Name

Cyclic nucleotide-gated ion channel subunit B

Annotation Symbol

CG17922

Feature Type

protein_coding_gene

FlyBase ID

FBgn0266346

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

57F9-57F9

Sequence location

2R:21,692,691..21,697,152 [-]

Recombination map

2-96

RefSeq locus

NT_033778 REGION:21692691..21697152

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

contributes_to intracellularly cyclic nucleotide-activated monoatomic cation channel activity

inferred from direct assay

(Finn et al., 1998)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables cGMP binding

inferred from biological aspect of ancestor with PANTHER:PTN000025739

(GO Reference Genome Project, 2011-)

enables intracellularly cAMP-activated cation channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000025739

(GO Reference Genome Project, 2011-)

enables intracellularly cGMP-activated cation channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000811708

(GO Reference Genome Project, 2011-)

enables intracellularly cyclic nucleotide-activated monoatomic cation channel activity

inferred from electronic annotation with InterPro:IPR050866

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:CngA; FB:FBgn0261612

(Kaupp and Seifert, 2002)

inferred from sequence or structural similarity with UniProtKB:Q24278

(Gene Ontology Curators, 2002-)

enables protein-containing complex binding

inferred from biological aspect of ancestor with PANTHER:PTN002900687

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in monoatomic cation transmembrane transport

inferred from direct assay

(Finn et al., 1998)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in monoatomic cation transmembrane transport

inferred from sequence or structural similarity with UniProtKB:Q24278

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000811708

(GO Reference Genome Project, 2011-)

involved_in monoatomic cation transport

inferred from sequence or structural similarity with FLYBASE:CngA; FB:FBgn0261612

(Kaupp and Seifert, 2002)

involved_in monoatomic ion transport

inferred from electronic annotation with InterPro:IPR005821

(InterPro Project Members, 2004-)

involved_in transmembrane transport

inferred from electronic annotation with InterPro:IPR005821

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of intracellular cyclic nucleotide activated cation channel complex

inferred from direct assay

(Finn et al., 1998)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of intracellular cyclic nucleotide activated cation channel complex

inferred from sequence or structural similarity with FLYBASE:CngA; FB:FBgn0261612

(Kaupp and Seifert, 2002)

inferred from biological aspect of ancestor with PANTHER:PTN000811708

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q24278

(Gene Ontology Curators, 2002-)

located_in membrane

inferred from electronic annotation with InterPro:IPR005821

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000811708

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CYCLIC NUCLEOTIDE-GATED CHANNEL SUBUNITS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYCLIC NUCLEOTIDE-GATED CHANNEL SUBUNITS -: Cyclic-nucleotide-gated channels (CNG) are non-selective cation channels activated by intracellular cAMP or cGMP. (Adapted from FBrf0149151 and FBrf0102034.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CngB for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W2D5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CngB-RA

FBtr0071675

NM_137763

3356

1040

CngB-RB

FBtr0342961

NM_001299764

3218

987

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CngB-PA

FBpp0071592

118.7

1040

9.11

FBpp0309732

112.6

987

8.96

A0A0B4LG93

NP_001286693

AHN56488

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CngB using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.71

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of intracellular cyclic nucleotide activated cation channel complex

inferred from direct assay

(Finn et al., 1998)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CngB in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CngB

View exonic expression by tissue for Dmel\CngB

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266346

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37479/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266346

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034656

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266346

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17922

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CngB

Transgenic constructs containing regulatory region of CngB

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

CngB^KK105029

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CngB^GD2132

viable, with Scer\GAL4^pnr-MD237

CngB^GD2132

viable, with Scer\GAL4^tin.CΔ4

CngB^GD2132

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (21)

Hsap\CNGB1

12 of 14

Yes

Hsap\CNGB3

12 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (18)

Rnor\Cngb1

12 of 14

Yes

Rnor\Cngb3

11 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (18)

Mmus\Cngb1

11 of 14

Yes

Mmus\Cngb3

11 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (22)

Xtro\cngb3

9 of 13

Yes

Xtro\cngb1

6 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (28)

Drer\cngb1a

11 of 14

Yes

Drer\cngb3.1

10 of 14

Yes

Drer\LOC562466

10 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\tax-2

12 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP011459

11 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (29)

Atha\CNGC13

3 of 13

Yes

Atha\CNGC14

3 of 13

Yes

Atha\CNGC15

3 of 13

Yes

Atha\CNGC17

3 of 13

Yes

Atha\CNGC18

3 of 13

Yes

Atha\DND1

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CngB. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (9)

CngA

6 of 13

Cngl

6 of 13

CG42260

5 of 13

4 of 13

eag

2 of 13

Elk

2 of 13

sei

2 of 13

CG6026

1 of 13

for

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

CNGB3; cyclic nucleotide gated channel subunit beta 3

model of achromatopsia 3

IEA

(FlyBase, 2019-)

CNGB1; cyclic nucleotide gated channel subunit beta 1

model of retinitis pigmentosa 45

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CNGB1; cyclic nucleotide gated channel subunit beta 1

12 of 14

600724

RETINITIS PIGMENTOSA 45; RP45

retinitis pigmentosa 45

CNGB3; cyclic nucleotide gated channel subunit beta 3

12 of 14

605080

ACHROMATOPSIA 3; ACHM3

achromatopsia 3

CNGA1; cyclic nucleotide gated channel subunit alpha 1

4 of 14

123825

RETINITIS PIGMENTOSA 49; RP49

retinitis pigmentosa 49

CNGA3; cyclic nucleotide gated channel subunit alpha 3

4 of 14

600053

ACHROMATOPSIA 2; ACHM2

achromatopsia 2

CNGA4; cyclic nucleotide gated channel subunit alpha 4

4 of 14

609472

CNGA2; cyclic nucleotide gated channel subunit alpha 2

3 of 14

300338

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266346

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-96

Cytogenetic map

57F9-57F9

Sequence location

2R:21,692,691..21,697,152 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57F9-57F9

Limits computationally determined from genome sequence between P{EP}Tim10^EP541&P{lacW}l(2)k09617^k09617 and P{PZ}l(2)07837⁰⁷⁸³⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-96

(FlyBase, 2016)

2-102.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

83018

y¹; M{WKO.1-B11}ZH-86Fb

26009

y¹ v¹; P{TRiP.JF02034}attP2

58072

y¹ v¹; P{TRiP.HMJ21964}attP40

NIG-Fly

HMJ21964

y¹ v¹; P{TRiP.HMJ21964}attP40/CyO

VDRC

v100882

P{KK105029}VIE-260B

v6599

w¹¹¹⁸ P{GD2132}v6599

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (18)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266346

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG17922 Cncβ

(FlyBase, 1992-)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: CngB CG17922

(FlyBase, 1992-)

Nomenclature comments

FBrf0102034 refers to, but doesn't identify, a Dmel CNG beta subunit as 'Cncβ'. This is almost certainly CG17922 because: i) the C. elegans ortholog is stated as 'tax-4' in FBrf0102034, and OrthoDB says CG17922 is the the tax-4 ortholog; ii) a later paper (FBrf0149151) identifies the CNG B subunit as CG17922; iii) a BLAST using human CNGB1 as the query gives CG17922 as the clear top hit. Using 'CngB' as the FlyBase symbol for the sake of consistency in gene nomenclature of the CNG family.

(FlyBase, 1992-)

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG17922

(Tsubouchi et al., 2012, Buffin and Gho, 2010, Ni et al., 2010.12.1, Vermehren-Schmaedick et al., 2010, Dijkers and O'Farrell, 2009, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Derré et al., 2007)

Cncβ

CngB

(Noyes and Davis, 2023, Lehmann et al., 2019, Meltzer et al., 2019)

dCNCβ

(Finn et al., 1998)

dmCNGB

(Kaupp and Seifert, 2002)

Name Synonyms

Cyclic nucleotide-gated ion channel subunit B

Cyclic-nucleotide-gated ion channel protein β-subunit

(Finn et al., 1998)

Secondary FlyBase IDs

FBgn0034656
FBgn0024813

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37479

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W2D5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W2D5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266346

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37479/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266346

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266346

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17922

MARRVEL_MODEL - MARRVEL (model organism gene)

37479

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266346

DroID - A comprehensive database of gene and protein interactions.

FBgn0266346

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034656

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0266346

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17922

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0266346

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014251

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (31)

The following external sites may use different assemblies or annotations than FlyBase.

-0.71

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