FB2025_02 , released April 17, 2025
Gene: Dmel\Pde8
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General Information
Symbol
Dmel\Pde8
Species
D. melanogaster
Name
Phosphodiesterase 8
Annotation Symbol
CG45019
Feature Type
FlyBase ID
FBgn0266377
Gene Model Status
Stock Availability
Enzyme Name (EC)
3',5'-cyclic-nucleotide phosphodiesterase (3.1.4.17)
3',5'-cyclic-GMP phosphodiesterase (3.1.4.35)
3',5'-cyclic-AMP phosphodiesterase (3.1.4.53)
Gene Summary
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (By similarity). Involved in the positive regulation of MAP kinase signaling and in inhibiting oxidative stress-induced cell death (PubMed:23509299). (UniProt, Q6NNF2)
Contribute a Gene Snapshot for this gene.
Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-104
RefSeq locus
NT_033778 REGION:23657567..23680844
Sequence
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
Biological Process (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from expression pattern
inferred from mutant phenotype
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from sequence model
inferred from biological aspect of ancestor with PANTHER:PTN001682918
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Protein Family (UniProt)
Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. (Q6NNF2)
Catalytic Activity (EC/Rhea)
3',5'-cyclic-AMP phosphodiesterase activity
3',5'-cyclic AMP + H2O = AMP + H(+) (3.1.4.53)
RHEA 25277: 3',5'-cyclic-nucleotide phosphodiesterase activity
a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+) (3.1.4.17)
RHEA 14653: 3',5'-cyclic-GMP phosphodiesterase activity
3',5'-cyclic GMP + H2O = GMP + H(+) (3.1.4.35)
RHEA 16957:
Summaries
Gene Group (FlyBase)
CYCLIC-NUCLEOTIDE PHOSPHODIESTERASES -
Cyclic-nucleotide phosphodiesterases are hydrolases that catalyze the hydrolysis of a nucleoside cyclic phosphate into a nucleoside phosphate.
Protein Function (UniProtKB)
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (By similarity). Involved in the positive regulation of MAP kinase signaling and in inhibiting oxidative stress-induced cell death (PubMed:23509299).
(UniProt, Q6NNF2)
Gene Model and Products
Number of Transcripts
12
Number of Unique Polypeptides
10

Please see the JBrowse view of Dmel\Pde8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q6NNF2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.48

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 6.33

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

Stop-codon suppression (UAG) postulated; FBrf0216884

Gene model reviewed during 5.53

A subset of the transcripts annotated for this gene are postulated to be circular mRNAs (FBrf0236596).

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0344180
3939
914
FBtr0344179
4331
904
FBtr0344176
2855
400
FBtr0344177
3982
805
FBtr0344178
5393
905
FBtr0344182
5562
914
FBtr0344183
5389
914
FBtr0344181
3939
949
FBtr0344184
4895
1050
FBtr0480522
1234
119
FBtr0480523
687
122
FBtr0480524
819
215
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
FBpp0310592
102.1
914
6.83
FBpp0310591
100.3
904
6.48
FBpp0310588
45.7
400
5.30
FBpp0310589
90.7
805
6.63
FBpp0310590
101.0
905
6.83
FBpp0310594
102.1
914
6.83
FBpp0310595
102.1
914
6.83
FBpp0310593
105.8
949
6.81
FBpp0310596
117.7
1050
6.61
FBpp0428313
12.5
119
9.11
FBpp0428314
12.9
122
9.11
FBpp0428315
23.0
215
9.79
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

914 aa isoforms: Pde8-PA, Pde8-PI, Pde8-PK
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pde8 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.43

Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult fat body

Comment: cyclical, peak ZT21-0

in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

Additional Descriptive Data

Expressed cyclically in the adult fat body.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pde8 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 23 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Pde8
Transgenic constructs containing regulatory region of Pde8
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Inferred from location ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (21)
13 of 14
Yes
Yes
12 of 14
No
Yes
4 of 14
No
No
4 of 14
No
No
4 of 14
No
No
1  
3 of 14
No
No
3 of 14
No
Yes
3 of 14
No
Yes
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
1  
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
1  
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
0  
2 of 14
No
No
2 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (21)
13 of 14
Yes
Yes
12 of 14
No
Yes
4 of 14
No
No
4 of 14
No
No
4 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
Yes
3 of 14
No
Yes
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
Yes
3 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
Mus musculus (laboratory mouse) (21)
13 of 14
Yes
Yes
12 of 14
No
Yes
4 of 14
No
No
4 of 14
No
No
4 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
Yes
3 of 14
No
Yes
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
Xenopus tropicalis (Western clawed frog) (33)
8 of 13
Yes
Yes
7 of 13
No
Yes
5 of 13
No
Yes
3 of 13
No
No
3 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Danio rerio (Zebrafish) (27)
13 of 14
Yes
Yes
11 of 14
No
Yes
4 of 14
No
No
4 of 14
No
No
4 of 14
No
No
4 of 14
No
No
3 of 14
No
No
3 of 14
No
Yes
3 of 14
No
Yes
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
No
3 of 14
No
Yes
3 of 14
No
No
3 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
1 of 14
No
Yes
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
9 of 14
Yes
Yes
3 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (8)
11 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (1)
5 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Pde8. Refer to their site for version information.
Paralogs
Paralogs (via DIOPT v9.1)
Drosophila melanogaster (Fruit fly) (5)
7 of 13
6 of 13
6 of 13
5 of 13
5 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Interaction Browsers

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    Interaction Browsers

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    External Data
    Linkouts
    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-104
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    59F5-59F6
    Limits computationally determined from genome sequence between P{PZ}apt03041 and P{EP}eglEP938
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (16)
    Genomic Clones (11)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (156)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments

        New annotation (CG42241) in release 5.7 of the genome annotation.

        Relationship to Other Genes
        Source for database merge of

        Source for merge of: Pde8 CG42241

        Additional comments

        Annotations CG5411 and CG42241 merged as CG45019 in release 5.53 of the genome annotation. Merge supported by RNA-seq junction data.

        Nomenclature History
        Source for database identify of

        Source for identity of: Pde8 CG5411

        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (7)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 105 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        References (65)