FlyBase Gene Report: Dmel\Pde8

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (By similarity). Involved in the positive regulation of MAP kinase signaling and in inhibiting oxidative stress-induced cell death (PubMed:23509299).

(UniProt, Q6NNF2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Pde8 or the JBrowse view of Dmel\Pde8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.48

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

gene_with_stop_codon_read_through ; SO:0000697

Stop-codon suppression (UAG) postulated; FBrf0216884

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

(FlyBase Genome Annotators, 2008)

protein_coding_gene

(FlyBase Genome Annotators, 2008)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pde8-RA

FBtr0344180

NM_166616

3939

914

Pde8-RB

FBtr0344179

NM_166618

4331

904

Pde8-RC

FBtr0344176

NM_166619

2855

400

Pde8-RD

FBtr0344177

NM_166617

3982

805

Pde8-RE

FBtr0344178

NM_137970

5393

905

Pde8-RI

FBtr0344182

NM_001169803

5562

914

Pde8-RK

FBtr0344183

NM_001169805

5389

914

Pde8-RN

FBtr0344181

NM_001169807

3939

949

Pde8-RO

FBtr0344184

NM_001169804

4895

1050

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Pde8-PA

FBpp0310592

102.1

914

6.83

NP_726350

AAM68263

Pde8-PB

FBpp0310591

100.3

904

6.48

NP_726352

AAM68265

Pde8-PC

FBpp0310588

45.7

400

5.30

NP_726353

AAM68266

Pde8-PD

FBpp0310589

90.7

805

6.63

NP_726351

AAM68264

Pde8-PE

FBpp0310590

101.0

905

6.83

NP_611814

AAF47038

Pde8-PI

FBpp0310594

102.1

914

6.83

NP_001163274

ACZ94546

Pde8-PK

FBpp0310595

102.1

914

6.83

NP_001163276

ACZ94548

Pde8-PN

FBpp0310593

105.8

949

6.81

NP_001163278

ACZ94550

Pde8-PO

FBpp0310596

117.7

1050

6.61

NP_001163275

ACZ94547

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

914 aa isoforms: Pde8-PA, Pde8-PI, Pde8-PK

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pde8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (6 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

3',5'-cyclic-AMP phosphodiesterase activity

inferred from sequence model

(Day, 2005)

3',5'-cyclic-nucleotide phosphodiesterase activity

inferred from electronic annotation with InterPro:IPR002073

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

mesoderm development

inferred from expression pattern

(Furlong et al., 2001)

negative regulation of hydrogen peroxide-induced cell death

inferred from mutant phenotype

(assigned by UniProt )

(Brown et al., 2013)

positive regulation of ERK1 and ERK2 cascade

inferred from mutant phenotype

(assigned by UniProt )

(Brown et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

cAMP metabolic process

inferred from sequence model

(Day, 2005)

positive regulation of ERK1 and ERK2 cascade

inferred from biological aspect of ancestor with PANTHER:PTN002602947

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

(Arbeitman et al., 2004)

adult stage

adult fat body

Comment: cyclical, peak ZT21-0

(Xu et al., 2011)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic anal pad

(Fisher et al., 2012)

embryonic stage 11 -- 12

salivary gland body primordium

(Fisher et al., 2012)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

Additional Descriptive Data

Expressed cyclically in the adult fat body.

(Xu et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pde8 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pde8

View exonic expression by tissue for Dmel\Pde8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266377

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5411

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 22 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pde8

Transgenic constructs containing regulatory region of Pde8

Deletions and Duplications ( 1 )

Disrupted in

Df(2R)Exel7180

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pde8^GD10547

viable, with Scer\GAL4^pnr-MD237

Pde8^GD10547

viable, with Scer\GAL4^tin.CΔ4

Pde8^GD10547

Sterility

Allele

fertile

Other Phenotypes

Allele

chemical sensitive

Pde8^del

circadian rhythm defective, with Scer\GAL4^P0.5.Pdf

Pde8^EY10946

flight defective, with Scer\GAL4^Mef2.PR

Pde8^GD10547

neuroanatomy defective, with Scer\GAL4^elav-C155

Pde8^EY10143

oxidative stress response defective

Pde8^del

pain response defective | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pde8^GD10547

Phenotype manifest in

Allele

synapse, with Scer\GAL4^elav-C155

Pde8^EY10143

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PDE8B

13 of 15

Yes

Hsap\PDE8A

11 of 15

Yes

Hsap\PDE9A

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Pde8b

13 of 15

Yes

Mmus\Pde8a

11 of 15

Yes

Mmus\Pde9a

1 of 15

Rattus norvegicus (Norway rat) (2)

Rnor\Pde8b

12 of 13

Yes

Rnor\Pde8a

11 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\pde8b

5 of 12

Yes

Xtro\pde8a

4 of 12

Yes

Danio rerio (Zebrafish) (4)

Drer\pde8b

12 of 15

Yes

Drer\pde8a

6 of 15

Yes

Drer\pde9a

1 of 15

Drer\pde9al

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\pde-6

9 of 15

Yes

Cele\Y119D3B.21

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\PDE2

2 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919022U )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Pde8

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18863

Drosophila persimilis

Dper\GL16875

Drosophila willistoni

Dwil\GK23066

Drosophila virilis

Dvir\GJ19994

Drosophila mojavensis

Dmoj\GI19028

Drosophila grimshawi

Dgri\GH20665

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500ST )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17025645

Musca domestica

House fly

Mdoa\17007311

Glossina morsitans

Tsetse fly

Gmor\GMOY010738

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09690

Mayetiola destructor

Hessian fly

Mdes\Mdes004895

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013342

Anopheles darlingi

American malaria mosquito

Adar\ADAC008749

Anopheles gambiae

Malaria mosquito

Agam\AGAP007163

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ010718

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005635

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02MS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA004107

Danaus plexippus

Monarch butterfly

Dple\DPOGS214302-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL005458

Apis florea

Little honeybee

Aflo\2244273188

Apis mellifera

Western honey bee

Amel\LOC408699

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP19634

Bombus terrestris

Buff-tailed bumblebee

Bter\15215063

Linepithema humile

Argentine ant

Lhum\LH22491

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00082d

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008590

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007101

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12655

Tribolium castaneum

Red flour beetle

Tcas\TC033870

Pediculus humanus

Human body louse

Phum\PHUM405220

Cimex lectularius

Bed bug

Clec\CLEC012044

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003782

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_17651

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02JZ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005636

Ixodes scapularis

Black-legged tick

Isca\ISCW014308

Ixodes scapularis

Black-legged tick

Isca\ISCW014309

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78041.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78040.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g04720

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_321680

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_321679

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0337 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pde8A

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pde8b

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000008111

Gallus gallus

Domestic chicken

Ggal\PDE8B

Gallus gallus

Domestic chicken

Ggal\PDE8A

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (5)

Gene Symbol

Score

Source

Alignment

dnc

3 of 10

2 of 10

2 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

PDE8B; phosphodiesterase 8B

model of striatonigral degeneration

IEA

(FlyBase, 2019-)

model of primary pigmented nodular adrenocortical disease

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PDE8B; phosphodiesterase 8B

13 of 15

603390

PDE8A; phosphodiesterase 8A

11 of 15

602972

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pde8 - βTub85D

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Jra|kay

suppressible

Pde8

(Franciscovich et al., 2008)

kay

enhanceable

Pde8

(Franciscovich et al., 2008)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266377

MIST (protein-protein) - An integrated Molecular Interaction Database

Pde8

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Purine metabolism

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

G alpha (s) signalling events

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-104

Cytogenetic map

59F5-59F5

Sequence location

2R:23,657,567..23,680,844 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

59F5-59F5

Limits computationally determined from genome sequence between P{PZ}apt⁰³⁰⁴¹ and P{EP}egl^EP938

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-104

(FlyBase, 2016)

2-108.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Bloomington

51892

y¹ v¹; P{TRiP.HMC03466}attP40

65635

w¹¹¹⁸; PBac{IT.GAL4}Pde8^1246-G4

44749

y¹ w^*; Mi{MIC}Pde8^MI08109

53157

y¹ w^*; Mi{MIC}Pde8^MI09719

53442

y¹ w^*; Mi{MIC}Pde8^MI10026

50814

y¹ w^*; P{PTT-GA}Pde8^CA07101

9775

y¹ w^*; P{UASp-YFP.Rab5}Pde8^08b

17642

y¹ w^67c23; P{EPgy2}Pde8^EY10143

20233

y¹ w^67c23; P{EPgy2}Pde8^EY10946

42080

y¹ w^*; Mi{MIC}Pde8^MI05352/SM6a

VDRC

v101413

P{KK109095}VIE-260B

v25976

w¹¹¹⁸; P{GD10547}v25976

More stocks available...

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (162)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266377

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37741

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Pde8 CG5411

(Day and Davies, 2003.6.10)

Source for database merge of

Source for merge of: Pde8 CG42241

(FlyBase Genome Annotators, 2013)

Additional comments

Annotations CG5411 and CG42241 merged as CG45019 in release 5.53 of the genome annotation. Merge supported by RNA-seq junction data.

(FlyBase Genome Annotators, 2013)

Other Comments

New annotation (CG42241) in release 5.7 of the genome annotation.

(FlyBase Genome Annotators, 2008)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 89 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37741

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q6NNF2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266377

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266377

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5411

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37741

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033870

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG45019

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266377

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Purine metabolism

MIST (protein-protein) - An integrated Molecular Interaction Database

Pde8

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

G alpha (s) signalling events

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG42241

(FlyBase Genome Annotators, 2008)

CG45019

(FlyBase Genome Annotators, 2013)

CG5411

(Aerts et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Mack et al., 2006, Day, 2005, Kim et al., 2005, Arbeitman et al., 2004, Berger et al., 2001, Furlong et al., 2001)

PDE8

(Davies et al., 2014, Brown et al., 2013, Clemens-Grisham et al., 2008, Day, 2005, Day and Davies, 2003.6.10)

Pde8

(Yildirim et al., 2019, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Li et al., 2015, Ashwal-Fluss et al., 2014, Belozerov et al., 2014, Jungreis et al., 2011, Xu et al., 2011, Aerts et al., 2010, Dahdal et al., 2010, Kadener et al., 2009, Franciscovich et al., 2008, Buszczak et al., 2007, Firth and Baker, 2007, Zhang et al., 2007, Kim et al., 2005)

pde8

(Franciscovich et al., 2008)

Name Synonyms

Phosphodiesterase 8

(Day and Davies, 2003.6.10)

Secondary FlyBase IDs

FBgn0034886
FBgn0250870

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (55)

Report Sections

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