FlyBase Gene Report: Dmel\Ote

General Information

Symbol

Dmel\Ote

Species

D. melanogaster

Name

Otefin

Annotation Symbol

CG5581

Feature Type

protein_coding_gene

FlyBase ID

FBgn0266420

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Otefin (Ote) encodes a nuclear membrane-associated protein that acts in concert with BMP/Dpp signaling to mediate bam transcriptional silencing. Its roles include oogenesis and germline stem cell self-renewal and differentiation. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
UniProtKB
Interactive Fly

Also Known As

hal

Key Links

Genomic Location

Cytogenetic map

55C2-55C2

Sequence location

2R:18,275,607..18,277,177 [-]

Recombination map

2-85

RefSeq locus

NT_033778 REGION:18275607..18277177

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:Lam; FB:FBgn0002525

(Goldberg et al., 1998)

enables transcription corepressor activity

inferred from mutant phenotype

(Jiang et al., 2008)

enables transcription factor binding

inferred from physical interaction with FLYBASE:Med; FB:FBgn0011655

(Jiang et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in germ-line stem cell population maintenance

inferred from mutant phenotype

(Jiang et al., 2008)

involved_in germ-line stem-cell niche homeostasis

inferred from mutant phenotype

(Barton et al., 2016)

involved_in negative regulation of transcription, DNA-templated

inferred from mutant phenotype

(Jiang et al., 2008)

involved_in nuclear membrane reassembly

inferred from direct assay

(Ashery-Padan et al., 1997)

involved_in oogenesis

inferred from high throughput mutant phenotype

(Schupbach and Wieschaus, 1991)

involved_in positive regulation of BMP signaling pathway

inferred from genetic interaction with FLYBASE:tkv; FB:FBgn0003716

(Jiang et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in endomembrane system

inferred from high throughput direct assay

(Tan et al., 2009)

located_in nuclear envelope lumen

inferred from direct assay

(assigned by UniProt )

(Harel et al., 1989)

located_in nuclear inner membrane

inferred from direct assay

(Ashery-Padan et al., 1997)

located_in nuclear membrane

inferred from direct assay

(Jiang et al., 2008)

located_in nuclear periphery

inferred from direct assay

(Dialynas et al., 2010)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Pathway (FlyBase)

Positive Regulators of BMP Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

Positive Regulators of BMP Signaling Pathway -: Positive regulators of Bone Morphogenetic Protein (BMP) signaling up-regulate the pathway, ultimately resulting in the increased nuclear activity of the Mad/Med transcription factor complex.

Protein Function (UniProtKB)

Inner nuclear membrane protein (PubMed:2186029, PubMed:9199347, PubMed:18410727, PubMed:22751930). Involved in the attachment of membrane vesicles to chromatin during nuclear assembly, and is probably required for centrosome maturation and cell cycle progression during mitosis (PubMed:9199347, PubMed:22751930). Essential for differentiation of certain tissues and the maintenance of progenitor cell populations (PubMed:18410727, PubMed:24700158, PubMed:23806619, PubMed:27174470). Required for the differentiation and maintenance of male and female germline stem cells (GSCs), as well as the maintenance of somatic cells in the GSC niche (PubMed:18410727, PubMed:23806619, PubMed:27174470). This role is likely to be independent of the BMP (Dpp) pathway that negatively regulates bam transcription during GSC differentiation (PubMed:18410727, PubMed:23806619). During development, plays essential and redundant functions with the other LEM domain proteins; bocks and MAN1 (PubMed:24700158). Also has a redundant but important role with bocks during larval development (PubMed:24700158).

(UniProt, P20240)

Summary (Interactive Fly)

a nuclear lamin that is essential for germline stem cell maintenance - physically interacts with Medea/Smad4 at the silencer element to regulate GSC fate

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ote for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P20240)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.44

Gene model reviewed during 5.39

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ote-RA

FBtr0086768

NM_057316

1571

424

Additional Transcript Data and Comments

Reported size (kB)

1.6 (northern blot)

(Padan et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ote-PA

FBpp0085947

46.6

424

9.99

P20240

NP_476664

AAF57722

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

406 (aa); 45 (kD predicted)

(Padan et al., 1990)

53 (kD observed)

(Harel et al., 1989)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Med (PubMed:18410727). Interacts with Lam (PubMed:9632815, PubMed:22751930). Interacts with aurA, alphaTub84B, gammaTub23C and gammaTub37C (PubMed:22751930).

(UniProt, P20240)

Post Translational Modification

Phosphorylation at Thr-63 by aurA may be required for exit from mitosis (PubMed:22751930). May be phosphorylated by Cdk1 and Pka-C1 (PubMed:9199347).

(UniProt, P20240)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P20240

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ote using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ote_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Padan et al., 1990)

Additional Descriptive Data

Ote transcript is present in all developmental stages. It is more abundant in embryos and in pupae.

(Padan et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & pupal stage

(Harel et al., 1989)

embryonic stage -- adult stage

(Ashery-Padan et al., 1997)

embryonic stage 12 -- 13

organism

(Harel et al., 1989)

oogenesis

(Ashery-Padan et al., 1997)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Using EM immunogold labeling, Ote protein was localized to the nucleoplasmic side of the inner nuclear membrane.

(Ashery-Padan et al., 1997)

Between stages S4 and S6-S7 of oogenesis, Ote protein localizes to the nuclear envelope of nurse cells, follicle cells and oocytes, but after stage S6-S7, nucleoplasmic and cytoplasmic accumulation is also observed. During embryogenesis, Ote protein is associated with the nucleus as well as with nonnuclear membrane vesicles. Like Lam protein, Ote protein is detected in the nuclei of most cells during larval, pupal and adult stages. Neither protein is detected in stages 6-11 of spermiogenesis. In larval and adult tissues, Ote pr tein localizes to the nuclear envelope.

(Ashery-Padan et al., 1997)

Ote protein is detected at high levels in embryos and during pupation. In embryos and tissue culture cells, Ote protein localizes to the periphery of the nucleus.

(Harel et al., 1989)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in endomembrane system

inferred from high throughput direct assay

(Tan et al., 2009)

located_in nuclear envelope lumen

inferred from direct assay

(assigned by UniProt )

(Harel et al., 1989)

located_in nuclear inner membrane

inferred from direct assay

(Ashery-Padan et al., 1997)

located_in nuclear membrane

inferred from direct assay

(Jiang et al., 2008)

located_in nuclear periphery

inferred from direct assay

(Dialynas et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ote in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ote

View exonic expression by tissue for Dmel\Ote

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266420

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37090/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266420

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003022

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266420

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5581

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ote

Transgenic constructs containing regulatory region of Ote

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2R)PC4

(Jiang et al., 2008, Doane, 1996, Tearle, 1996.5.14, Schupbach and Wieschaus, 1991)

Df(2R)Pcl^XM82

(Tearle, 1996.5.14)

Gene not disrupted in

Df(2R)Pcl7B

(Tearle, 1996.5.14)

Df(2R)Pcl11B

(Tearle, 1996.5.14)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ote^GD2067

viable, with Scer\GAL4^Mef2.PR

Ote^GD2067

viable, with Scer\GAL4^pnr-MD237

Ote^GD2067

viable (with Ote^B279)

Ote^PK

viable (with Ote^PK)

Ote^B279

Sterility

Allele

female sterile

female sterile (with Ote^B279)

Ote^PK

female sterile (with Ote^PK)

Ote^B279

male semi-sterile | progressive (with Ote^B279)

Ote^PK

male semi-sterile | progressive (with Ote^PK)

Ote^B279

Other Phenotypes

Allele

abnormal cell adhesion | adult stage (with Ote^B279)

Ote^PK

abnormal cell adhesion | adult stage (with Ote^PK)

Ote^B279

abnormal developmental rate | female | larval stage (with Ote^B279)

Ote^PK

abnormal developmental rate | female | larval stage (with Ote^PK)

Ote^B279

abnormal size | third instar larval stage (with Ote^B279)

Ote^PK

abnormal size | third instar larval stage (with Ote^PK)

Ote^B279

decreased cell number | adult stage | conditional, with Scer\GAL4^VP16.nos.UTR

Ote^{miRNA.2XartmiR.UASp}

decreased cell number | adult stage | progressive (with Ote^B279)

Ote^PK

decreased cell number | adult stage | progressive (with Ote^PK)

Ote^B279

decreased fecundity | female (with Ote^B279)

Ote^PK

decreased fecundity | female (with Ote^PK)

Ote^B279

decreased fecundity | male | progressive (with Ote^B279)

Ote^PK

decreased fecundity | male | progressive (with Ote^PK)

Ote^B279

decreased size | adult stage | female (with Ote^B279)

Ote^PK

decreased size | adult stage | female (with Ote^PK)

Ote^B279

increased cell death | larval stage, with Scer\GAL4^GMR.PS

Ote^GD2067

visible | adult stage, with Scer\GAL4^GMR.PS

Ote^GD2067

Phenotype manifest in

Allele

abdominal 1 lateral transverse muscle cell, with Scer\GAL4^Mef2.PU

Ote^HMS01926

egg

Ote^unspecified

escort cell | adult stage (with Ote^B279)

Ote^PK

escort cell | adult stage (with Ote^PK)

Ote^B279

eye, with Scer\GAL4^GMR.PS

Ote^GD2067

eye disc, with Scer\GAL4^GMR.PS

Ote^GD2067

female germline cell (with Ote^B279)

Ote^PK

female germline cell (with Ote^PK)

Ote^B279

female germline cell | adult stage (with Ote^B279)

Ote^PK

female germline cell | adult stage (with Ote^PK)

Ote^B279

female germline cell | larval stage

Ote^unspecified

female germline stem cell

Ote^EMS

female germline stem cell, with Scer\GAL4^VP16.nos.UTR

Ote^{miRNA.2XartmiR.UASp}

female germline stem cell | adult stage (with Ote^B279)

Ote^PK

female germline stem cell | adult stage (with Ote^PK)

Ote^B279

female germline stem cell | germline clone

female germline stem cell | increased number

Ote^nos.PJ

fusome

fusome (with Df(2R)ED3636)

Ote^B279

fusome (with Ote^B279)

fusome (with Ote^EMS)

fusome (with Ote^PK)

germarium

germarium, with Scer\GAL4^VP16.nos.UTR

Ote^{miRNA.2XartmiR.UASp}

germarium (with Df(2R)ED3636)

Ote^B279

germarium (with Ote^B279)

germarium (with Ote^EMS)

germarium (with Ote^PK)

germarium cap cell | adult stage | progressive (with Ote^B279)

Ote^PK

germarium cap cell | adult stage | progressive (with Ote^PK)

Ote^B279

germinal proliferation center hub | adult stage | progressive (with Ote^B279)

Ote^PK

germinal proliferation center hub | adult stage | progressive (with Ote^PK)

Ote^B279

hub cell | adult stage (with Ote^B279)

Ote^PK

hub cell | adult stage (with Ote^PK)

Ote^B279

male germline cell | adult stage (with Ote^B279)

Ote^PK

male germline cell | adult stage (with Ote^PK)

Ote^B279

male germline cell | third instar larval stage (with Ote^B279)

Ote^PK

male germline cell | third instar larval stage (with Ote^PK)

Ote^B279

male germline stem cell | adult stage (with Ote^B279)

Ote^PK

male germline stem cell | adult stage (with Ote^PK)

Ote^B279

muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^Mef2.PU

Ote^HMS01926

muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^twi.PU

Ote^HMS01926

nucleus, with Scer\GAL4^Mef2.PU

Ote^HMS01926

nucleus, with Scer\GAL4^twi.PU

Ote^HMS01926

ovary

Ote^EMS

ovary, with Scer\GAL4^VP16.nos.UTR

Ote^{miRNA.2XartmiR.UASp}

ovary | adult stage (with Ote^B279)

Ote^PK

ovary | adult stage (with Ote^PK)

Ote^B279

spectrosome

spectrosome (with Df(2R)ED3636)

Ote^B279

spectrosome (with Ote^B279)

spectrosome (with Ote^EMS)

spectrosome (with Ote^PK)

terminal filament | larval stage (with Ote^B279)

Ote^PK

terminal filament | larval stage (with Ote^PK)

Ote^B279

testis | third instar larval stage (with Ote^B279)

Ote^PK

testis | third instar larval stage (with Ote^PK)

Ote^B279

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (0)

No records found.

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (0)

No records found.

Rattus norvegicus (Norway rat) (0)

No records found.

Xenopus tropicalis (Western clawed frog) (0)

No records found.

Danio rerio (Zebrafish) (0)

No records found.

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ote - Brca2

ion exchange chromatography, peptide massfingerprinting

(Kusch, 2015)

Ote - CG10214

experimental knowledge based

(Guruharsha et al., 2011)

Ote - CG2199

experimental knowledge based

(Guruharsha et al., 2011)

Ote - CG4407

experimental knowledge based

(Guruharsha et al., 2011)

Ote - Gnf1

experimental knowledge based

(Guruharsha et al., 2011)

Ote - Hmgs

experimental knowledge based

(Guruharsha et al., 2011)

Ote - JMJD6

experimental knowledge based

(Guruharsha et al., 2011)

Ote - Lam

two hybrid, experimental knowledge based, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Habermann et al., 2012, Guruharsha et al., 2011, Goldberg et al., 1998)

Ote - Med

anti tag coimmunoprecipitation, anti tag western blot, western blot, anti bait coimmunoprecipitation, fluorescent resonance energy transfer, fluorescence microscopy

(Jiang et al., 2008)

Ote - Mer

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Ote - Nemp

anti tag coimmunoprecipitation, anti tag western blot

(Tsatskis et al., 2020)

Ote - Sumo

anti tag coimmunoprecipitation, comigration in sds page, anti tag western blot

(Pirone et al., 2017)

Ote - aurA

anti tag coimmunoprecipitation, western blot

(Habermann et al., 2012)

Ote - baf

two hybrid

(Pinto et al., 2008)

Ote - bocks

experimental knowledge based

(Guruharsha et al., 2011)

Ote - cnn

anti bait coimmunoprecipitation, peptide massfingerprinting

(Habermann et al., 2012)

Ote - γTub23C

anti tag coimmunoprecipitation, western blot

(Habermann et al., 2012)

Ote - γTub37C

anti tag coimmunoprecipitation, western blot

(Habermann et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ote

enhanceable

bocks

(Barton et al., 2014)

Ote

suppressible

bocks

(Barton et al., 2014)

Ote

suppressible

Diap1

(Tanaka et al., 2019)

Ote

enhanceable

smash

(Tanaka et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

smash

enhanceable

Ote

(Tanaka et al., 2019)

tkv

suppressible

Ote

(Jiang et al., 2008)

External Data

Subunit Structure (UniProtKB)

Interacts with Med (PubMed:18410727). Interacts with Lam (PubMed:9632815, PubMed:22751930). Interacts with aurA, alphaTub84B, gammaTub23C and gammaTub37C (PubMed:22751930).

(UniProt, P20240 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266420

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Positive Regulators of BMP Signaling Pathway -: Positive regulators of Bone Morphogenetic Protein (BMP) signaling up-regulate the pathway, ultimately resulting in the increased nuclear activity of the Mad/Med transcription factor complex.

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-85

Cytogenetic map

55C2-55C2

Sequence location

2R:18,275,607..18,277,177 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55C2-55C2

Limits computationally determined from genome sequence between P{PZ}Hsf⁰³⁰⁹¹&P{EP}Dgp-1^EP731 and P{PZ}sbb⁰⁴⁵²⁵&P{EP}EP1081^EP1081

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

55C-55C

(determined by in situ hybridisation)

(Padan et al., 1990)

Experimentally Determined Recombination Data

Location

2-85

(FlyBase, 2016)

2-89.3

(Comeron et al., 2012)

2-[86]

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

39009

y¹ v¹; P{TRiP.HMS01926}attP40

5092

cn¹ Ote^DB bw¹/CyO, l(2)DTS513¹

16189

w¹¹¹⁸; PBac{5HPw⁺}Ote^B279/CyO, P{sevRas1.V12}FK1

62262

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05269}attP40

Kyoto

108036

cn¹ Ote^DB bw¹/CyO, l(2)DTS513¹

VDRC

v105308

P{KK109866}VIE-260B

v4983

w¹¹¹⁸; P{GD2067}v4983

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (178)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD41911

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266420

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37090

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Unnikannan et al., 2020, Barton et al., 2014, Jiang et al., 2008, Wagner et al., 2004, Padan et al., 1990)

monoclonal

(Harel et al., 1989)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Ote hal

(Barton et al., 2013)

Additional comments

Other Comments

Ote is required autonomously for the survival of germline stem cells and non-autonomously for the maintenance of their somatic niche in both males and females.

(Barton et al., 2016)

The absence of Ote function does not cause female germ cell loss through activation of bam transcription. The primary defect in mutant female germline stem cells is a block of differentiation, which ultimately leads to germ cell death.

(Barton et al., 2013)

Lam protein interacts with both Ote and fs(1)Ya in a yeast two-hybrid system.

(Goldberg et al., 1998)

Ote behaves as a peripheral protein and is localised to the nucleoplasmic side of the nuclear envelope. Expression studies reveal the C-terminal 11 amino acid hydrophobic sequence is essential for targeting Ote to the nuclear periphery.

(Ashery-Padan et al., 1997)

Characterisation of Ote suggests an essential role in the assembly of the nuclear envelope.

(Ashery-Padan et al., 1997)

The hal locus affects early oogenesis: mutations cause the production of few, defective germ cells.

(Schupbach and Wieschaus, 1991)

Ote has been cloned and sequenced.

(Padan et al., 1990)

Ote protein is not a component of nuclear pore complexes. Some Ote protein is apparently retained in an envelope-like structure throughout mitosis in early Drosophila embryos.

(Harel et al., 1989)

Origin and Etymology

Discoverer

Etymology

The name 'otefin' is a transliteration of the Hebrew word meaning 'envelopes'.

(Harel et al., 1989)

Identification

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37090

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P20240

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P20240

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P20240

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266420

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266420

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37090/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266420

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ote_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003022

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266420

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5581

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37090

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5581

MARRVEL_MODEL - MARRVEL (model organism gene)

37090

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266420

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG5581

(Kolkhof et al., 2017, Çiçek et al., 2016, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Haussmann et al., 2008)

Otefin

(Kusch, 2015)

hal

(Barton et al., 2013)

ote

(Tsatskis et al., 2020, Tanaka et al., 2019, Barton et al., 2016, Barton et al., 2013, Sui and Yang, 2011, Wang et al., 2011, Jiang et al., 2008, Wilmington et al., 2008)

otefin

(Pałka et al., 2018, Wagner et al., 2004)

Name Synonyms

Otefin

(Duan et al., 2020, Tsatskis et al., 2020, Warecki et al., 2020, Pirone et al., 2017, Jokhi et al., 2013, Rohrbaugh et al., 2013, Milon et al., 2012, Harris and Ashe, 2011, Dialynas et al., 2010, Somma et al., 2008, Wilmington et al., 2008, Lancaster et al., 2007, Berman et al., 2004)

emerin

(Kitzman et al., 2022)

halted

haulted

(Mohr and Gelbart, 2002)

otefin

(Busayavalasa et al., 2012, Habermann et al., 2012, Furukawa et al., 2009, Haussmann et al., 2008, Jiang et al., 2008, Pinto et al., 2008, Wagner et al., 2006, McHugh et al., 2004, Wagner et al., 2004, Cohen et al., 2001, Hita et al., 1999, Goldberg et al., 1998, Ashery-Padan et al., 1997, Padan et al., 1990, Harel et al., 1989)

Secondary FlyBase IDs

FBgn0003022
FBgn0001173

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (108)

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