hal
a nuclear lamin that is essential for germline stem cell maintenance - physically interacts with Medea/Smad4 at the silencer element to regulate GSC fate
Please see the JBrowse view of Dmel\Ote for information on other features
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Gene model reviewed during 5.50
Gene model reviewed during 5.44
Gene model reviewed during 5.39
1.6 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
406 (aa); 45 (kD predicted)
53 (kD observed)
Interacts with Med (PubMed:18410727). Interacts with Lam (PubMed:9632815, PubMed:22751930). Interacts with aurA, alphaTub84B, gammaTub23C and gammaTub37C (PubMed:22751930).
Phosphorylation at Thr-63 by aurA may be required for exit from mitosis (PubMed:22751930). May be phosphorylated by Cdk1 and Pka-C1 (PubMed:9199347).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ote using the Feature Mapper tool.
Comment: maternally deposited
Ote transcript is present in all developmental stages. It is more abundant in embryos and in pupae.
Using EM immunogold labeling, Ote protein was localized to the nucleoplasmic side of the inner nuclear membrane.
Between stages S4 and S6-S7 of oogenesis, Ote protein localizes to the nuclear envelope of nurse cells, follicle cells and oocytes, but after stage S6-S7, nucleoplasmic and cytoplasmic accumulation is also observed. During embryogenesis, Ote protein is associated with the nucleus as well as with nonnuclear membrane vesicles. Like Lam protein, Ote protein is detected in the nuclei of most cells during larval, pupal and adult stages. Neither protein is detected in stages 6-11 of spermiogenesis. In larval and adult tissues, Ote pr tein localizes to the nuclear envelope.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Ote in GBrowse 22-85
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
Source for merge of: Ote hal
Ote is required autonomously for the survival of germline stem cells and non-autonomously for the maintenance of their somatic niche in both males and females.
Characterisation of Ote suggests an essential role in the assembly of the nuclear envelope.
The hal locus affects early oogenesis: mutations cause the production of few, defective germ cells.
Ote has been cloned and sequenced.
The name 'otefin' is a transliteration of the Hebrew word meaning 'envelopes'.