General Information
Symbol
Dmel\kis
Species
D. melanogaster
Name
kismet
Annotation Symbol
CG3696
Feature Type
FlyBase ID
FBgn0266557
Gene Model Status
Stock Availability
Enzyme Name (EC)
Adenosinetriphosphatase (3.6.1.3)
Gene Snapshot
Kismet (Kis) is a chromodomain containing ATP-dependent transcription factor that controls gene expression through epigenetic mechanisms. Kis roles include developmental patterning of the embryo, cell proliferation, eye development, synaptic transmission, axonal pruning, and memory. [Date last reviewed: 2016-09-01]
Also Known As
l(2)k16510, KIS-L
Genomic Location
Cytogenetic map
Sequence location
2L:210,735..250,823 [-]
Recombination map
2-0.5
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Experimental Evidence
-
Predictions / Assertions
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O = ADP + phosphate (3.6.1.3)
Summaries
Gene Group Membership
CHROMODOMAIN HELICASES -
The chromodomain helicase DNA-binding (CHD) family is defined by the presence of tandem N-terminal chromodomains and an SNF2-like ATPase domain. These ATP-dependent chromatin remodellers are usually components of remodelling complexes. (Adapted from FBrf0228973 and FBrf0228925).
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
kis: kismet (J.A. Kennison)
Isolated as a dominant suppressor of Pc alleles. Homozygous lethal. Duplications for kismet strongly enhance the dominant Pc phenotypes. kismet mutations suppress the antennal transformations of AntpB, Antp73b, and AntpNs, as well as the transformations caused by ectopic expression of Antp protein under the control of a heat-inducible promoter. kismet mutations in mitotic clones induced during larval stages transform the fifth abdominal segment into a more anterior segmental identity.
kisS: kismet-Spradling
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
6

Please see the GBrowse view of Dmel\kis or the JBrowse view of Dmel\kis for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.48
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.39
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0078144
17943
5322
FBtr0078145
8225
2151
FBtr0299837
18528
5517
FBtr0308253
18006
5343
FBtr0308254
17733
5252
FBtr0308255
17731
5191
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0077803
573.6
5322
5.52
FBpp0077804
224.7
2151
6.10
FBpp0289115
593.4
5517
5.34
FBpp0300573
575.9
5343
5.56
FBpp0300574
566.1
5252
5.53
FBpp0300575
560.9
5191
5.53
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kis using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (22 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000330
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR031053
(assigned by InterPro )
inferred from sequence or structural similarity with HGNC:20153
Biological Process (17 terms)
Terms Based on Experimental Evidence (16 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR031053
(assigned by InterPro )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
kis protein is observed in third instar larvae in multiple motor neurons within the ventral nerve cord. It is also seen in multinucleated postsynaptic muscles.
kis protein is broadly expressed in larval central nervous system, including in the brain lobes, the cortex, and the ventral nerve cord. kis expression is strongly colocalized with ato and da protein in these tissues.
kis protein expression is observed anterior to, within and posterior to the morphogenetic furrow in the developing retina.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
Expression Deduced from Reporters
Reporter: P{PZ}kis07812
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
adult brain cell body rind

Comment: cell bodies in adult brain cortex and glial sheath

adult gnathal ganglion | restricted

Comment: part of adult subesophageal ganglion

Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\kis in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 145 )
For All Classical and Insertion Alleles Show
 
Allele of kis
Class
Mutagen
Associated Insertion
Stocks
Known lesion
Other relevant insertions
insertion of mobile activating element
Name
Expression Data
miscellaneous insertions
Transgenic Constructs ( 10 )
Deletions and Duplications ( 14 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
9 of 15
No
Yes
9 of 15
No
Yes
9 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
9 of 15
No
Yes
9 of 15
No
Yes
7 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (5)
9 of 13
Yes
Yes
8 of 13
No
Yes
6 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (5)
5 of 12
Yes
Yes
5 of 12
Yes
Yes
5 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (6)
10 of 15
Yes
Yes
8 of 15
No
Yes
7 of 15
No
Yes
5 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
11 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
3 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
1 of 12
Yes
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919002M )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915001G )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W001I )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X002A )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G003J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ciona intestinalis
Vase tunicate
Human Disease Model Data
FlyBase Human Disease Model Reports
Alleles Reported to Model Human Disease (Disease Ontology)
Download
Models ( 4 )
Allele
Disease
Evidence
References
model of  CHARGE syndrome
in combination with kisLM27
inferred from mutant phenotype
model of  CHARGE syndrome
inferred from mutant phenotype
model of  CHARGE syndrome
inferred from mutant phenotype
model of  CHARGE syndrome
inferred from mutant phenotype
in combination with kisk13416
Interactions ( 3 )
Comments ( 0 )
 
Human Orthologs (via DIOPT v7.1)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map
2-0.5
Cytogenetic map
Sequence location
2L:210,735..250,823 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
21B4-21B5
Limits computationally determined from genome sequence between P{lacW}spenk06805&P{lacW}l(2)k13604k13604 and P{lacW}U2af38k14504
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Location from complementation analysis with deficiency and duplication chromosomes (details unspecified).
21B4-21B6
(determined by in situ hybridisation) 21C1--2 (determined by in situ hybridisation)
21B-21B
(determined by in situ hybridisation)
21B4-21B6
21B4--7 21B6--7 21B7--8 21C1--2
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (58)
Genomic Clones (30)
cDNA Clones (82)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of
Source for identity of: kis CG3696
Source for database merge of
Source for merge of: kis anon-WO0172774.164 anon-WO0257455.5
Source for merge of: kis l(2)k13631 l(2)k14112
Source for merge of: kis BEST:GM02209
Source for merge of: kis l(2)k16510
Source for merge of: kis l(2)k11324
Source for merge of: kis CG3660 CG18326
Source for merge of: kis EP474
Additional comments
snoRNA:kis-a is encoded in an intron of kis.
mir-965 is located within kis.
Annotations CG3696, CG3660 and CG18326 merged as CG3696 in release 3 of the genome annotation.
Source for merge of kis anon-WO0172774.164 anon-WO0257455.5 was sequence comparison ( date:051113 ).
Source for merge of kis l(2)k11324 was sequence comparison ( date:050921 ).
Other Comments
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.
Loss of zygotic kis function causes homeotic transformations. Loss of maternal kis function causes segmentation defects similar to those caused by mutations in pair-rule genes.
Three EMS-induced alleles have been isolated that weakly suppress the bristle and wing vein phenotypes of NAx-16.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.
Identified in a screen for modifiers of the Dfd13/Dfd3 mutant phenotype. Mutants also interact with Pc.
kis is required for the function of other homeotic genes.
Sections of the Scr regulatory region may be important for regulation of Scr by Polycomb- and trithorax-group genes.
kis is one of the 18 loci identified in a screen for dominant modifiers of Pc and/or Antp phenotypes. Mosaic analysis with mutant alleles reveals homeotic transformations in adult cuticle.
Isolated as a dominant suppressor of Pc alleles. Homozygous lethal. Duplications for kismet strongly enhance the dominant Pc phenotypes. kismet mutations suppress the antennal transformations of AntpB, Antp73b and AntpNs, as well as the transformations caused by ectopic expression of Antp protein under the control of a heat-inducible promoter. kismet mutations in mitotic clones induced during larval stages transform the fifth abdominal segment into a more anterior segmental identity.
Origin and Etymology
Discoverer
Kennison, 1984.
Etymology
Identification
External Crossreferences and Linkouts ( 85 )
Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Linkouts
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
DroID - A comprehensive database of gene and protein interactions.
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
KEGG Genes - Molecular building blocks of life in the genomic space.
Synonyms and Secondary IDs (38)
Reported As
Symbol Synonym
BEST:GM02209
CG18326
EP474
FBtr0078144
Su(Pc)21AB
anon-WO0172774.164
anon-WO0257455.5
l(2)07812
l(2)k08827
l(2)k11324
l(2)s3527
l(2)s4771
l(2)s4793
Secondary FlyBase IDs
  • FBgn0062647
  • FBgn0086902
  • FBgn0010642
  • FBgn0010689
  • FBgn0010697
  • FBgn0010698
  • FBgn0021918
  • FBgn0026596
  • FBgn0031234
  • FBgn0031236
  • FBgn0046436
  • FBgn0063789
  • FBgn0021813
  • FBgn0001309
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (217)