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General Information
Symbol
Dmel\Hsc70-4
Species
D. melanogaster
Name
Heat shock protein cognate 4
Annotation Symbol
CG4264
Feature Type
FlyBase ID
FBgn0266599
Gene Model Status
Stock Availability
Enzyme Name (EC)
Adenosinetriphosphatase (3.6.1.3)
Gene Snapshot
Heat shock protein cognate 4 (Hsc70-4) encodes a protein involved in protein folding. It contributes to clathrin-mediated endocytosis, neurotransmitter exocytosis and RNAi response. [Date last reviewed: 2019-09-12]
Also Known As
Hsc4, Hsc70, BAP74, Hsp70, scattered
Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:15,242,687..15,246,614 [+]
Recombination map
3-56
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the heat shock protein 70 family. (P11147)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O = ADP + phosphate (3.6.1.3)
Summaries
Gene Group (FlyBase)
HEAT SHOCK PROTEIN 70 CHAPERONES -
The Heat Shock Protein 70 (Hsp70) superfamily of chaperones assist in numerous folding processes and are upregulated by heat stress and toxic chemicals. Hsp70 chaperones share a highly conserved bipartite domain structure composed of an ATPase domain and a substrate-binding domain. (Adapted from PMID:17441502, FBrf0232269 and FBrf0174945).
Gene Model and Products
Number of Transcripts
7
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Hsc70-4 or the JBrowse view of Dmel\Hsc70-4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.48
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0083057
2368
651
FBtr0083060
2339
651
FBtr0083058
2280
651
FBtr0083059
2352
651
FBtr0083055
2284
651
FBtr0083056
2359
651
FBtr0337053
2336
651
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0082516
71.1
651
5.16
FBpp0082519
71.1
651
5.16
FBpp0082517
71.1
651
5.16
FBpp0082518
71.1
651
5.16
FBpp0082514
71.1
651
5.16
FBpp0082515
71.1
651
5.16
FBpp0307982
71.1
651
5.16
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hsc70-4 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (38 terms)
Molecular Function (8 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:P38646
Biological Process (20 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:P38646
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002500132
(assigned by GO_Central )
Cellular Component (10 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
colocalizes_with Z disc
inferred from direct assay
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002500132
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:P38646
inferred from biological aspect of ancestor with PANTHER:PTN002500132
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002500132
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism | ubiquitous

Comment: maternally deposited

Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Hsc70-4 protein is expressed in all cells throughout embryonic development.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
colocalizes_with Z disc
inferred from direct assay
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
adult brain

Comment: expression throughout adult brain

High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Hsc70-4 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 39 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Hsc70-4
Transgenic constructs containing regulatory region of Hsc70-4
Deletions and Duplications ( 9 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
wing vein L3 & sensillum campaniformium | ectopic
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (13)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
8 of 15
No
Yes
7 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
 
4 of 15
No
No
 
4 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
Yes
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
8 of 15
Yes
No
7 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
4 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (11)
10 of 13
Yes
Yes
7 of 13
Yes
No
5 of 13
No
Yes
4 of 13
No
No
4 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (10)
10 of 12
Yes
Yes
7 of 12
Yes
No
5 of 12
Yes
No
5 of 12
Yes
No
4 of 12
No
Yes
4 of 12
Yes
No
3 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (15)
12 of 15
Yes
Yes
11 of 15
Yes
No
10 of 15
Yes
No
7 of 15
Yes
No
5 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
No
3 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (10)
12 of 15
Yes
Yes
4 of 15
Yes
No
4 of 15
No
No
4 of 15
No
No
3 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (10)
9 of 9
Yes
Yes
8 of 9
No
No
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (8)
13 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
11 of 15
No
Yes
5 of 15
No
No
4 of 15
No
Yes
4 of 15
No
Yes
2 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (5)
11 of 12
Yes
Yes
11 of 12
Yes
Yes
3 of 12
No
No
3 of 12
No
Yes
1 of 12
No
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091904X1 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502RJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02J2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02GG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03SF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (13)
8 of 10
6 of 10
6 of 10
6 of 10
6 of 10
6 of 10
6 of 10
5 of 10
5 of 10
5 of 10
3 of 10
2 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 6 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-56
    Cytogenetic map
    Sequence location
    3R:15,242,687..15,246,614 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    88E4-88E4
    Limits computationally determined from genome sequence between P{EP}CG33967EP666 and P{lacW}Hsc70-4L3929
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    88E8-88E9
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Notes
    Stocks and Reagents
    Stocks (26)
    Genomic Clones (16)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (1546)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: Hsc70-4 CG4264
    Source for database merge of
    Source for merge of: scattered Hsc70-4
    Source for merge of: Hsc70-4 l(3)L3929
    Source for merge of: Hsc70-4 anon-WO0118547.237
    Additional comments
    The Hsc70-4 gene may have been derived from the Hsc70-3 gene by retroposition.
    The Hsc70-4 gene may have been derived from the Hsc70-1 gene by retroposition.
    Source for merge of Hsc70-4 anon-WO0118547.237 was sequence comparison ( date:051113 ).
    Other Comments
    The Hsc70-4 protein has two separable functions: chaperone activity and membrane deformation activity. The membrane deforming activity of Hsc70-4 requires oligomerisation and membrane association. The factors that promote the chaperone activity of Hsc70-4 (ATP and the presence of the Sgt cochaperone) simultaneously inhibit its membrane deforming activity. The membrane deforming activity of Hsc70-4 is necessary and sufficient for endosomal microautophagy at synapses, which promotes the turnover of a number of synaptic proteins. Both the chaperone activity and membrane deformation activity of Hsc70-4 are needed to maintain normal levels of synaptic transmission.
    Hsc70-4 mutation disrupts clathrin function and affects early steps of the endocytic pathway.
    Interacts gentically with: Fmr1
    sev-mediated internalization of boss protein is blocked in mutant Hsc70-4 eye disc epithelial cells.
    Hsc70-4 is critical for neurotransmitter exocytosis.
    Area matching Drosophila EST AI294469.
    The gene products of Hsp83 and the Hsc70 genes are present in a chaperone complex required for activation of DNA binding by the EcR/usp complex. The gene products of Hsp83 and the Hsc70 genes are also required in vivo for EcR activity and EcR is the primary target of the chaperone complex.
    Hsc70-4 is thought to be involved in subcellular trafficking and may affect the number of functional N receptors on the cell surface.
    Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell dysregulation.
    Isolated during a screen for mutations that disrupt Bolwig's organ or Bolwig's nerve development.
    The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.
    Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function.
    First recognised by sequence similarity to Hsp70. Transcription is developmentally regulated, but is temperature independent.
    transcript abundance embryo: ++++ transcript abundance larva: ++++ transcript abundance adult: ++++
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 90 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (50)
    Reported As
    Symbol Synonym
    Bap74
    Hsc70-4
    (Thomas et al., 2018, Tsuboyama et al., 2018, Coyne et al., 2017, Dyer et al., 2017, Galluzzi et al., 2017, Miller et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Tan et al., 2016, Yu et al., 2016, Dent et al., 2015, Horan et al., 2015, Iwasaki et al., 2015, Jia et al., 2015, Štětina et al., 2015, Tang et al., 2015, Xie et al., 2015, Ashwal-Fluss et al., 2014, Benbahouche et al., 2014, dos Santos, 2014, Kim and Choe, 2014, Lee et al., 2014, Mukherjee et al., 2014, Radermacher et al., 2014, Slawson et al., 2014, Colinet et al., 2013, Kockmann et al., 2013, Kwon et al., 2013, O'Sullivan et al., 2013, Telonis-Scott et al., 2013, Yin et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Franco et al., 2011, Friedman et al., 2011, Rees et al., 2011, Toku et al., 2011, Wertheim et al., 2011, Iwasaki et al., 2010, Kallappagoudar et al., 2010, Kim et al., 2010, Müller et al., 2010, Wasbrough et al., 2010, Zhang et al., 2010, Ahlander and Bosco, 2009, Gruenewald et al., 2009, Herold et al., 2009, Pisa et al., 2009, Takemori and Yamamoto, 2009, Worringer et al., 2009, Allen and Spradling, 2008, Bettencourt et al., 2008, Puri et al., 2008, Bai et al., 2007, Buszczak et al., 2007, Derré et al., 2007, Nekrasov et al., 2007, Stuart et al., 2007, Bandyopadhyay et al., 2006, Beller et al., 2006, Beller et al., 2006, Cermelli et al., 2006, Chartier et al., 2006, Dorner et al., 2006, Hagedorn et al., 2006, Hennig et al., 2006, Molnar et al., 2006, Neal et al., 2006, Walser et al., 2006, Rehwinkel et al., 2004, Stanyon et al., 2004)
    Q3KN45_DROME
    anon-WO0118547.237
    chr3R:11070155..11070315
    l(3)03550
    l(3)j7A4
    Name Synonyms
    Bap74
    Heat shock 70 kDa protein cognate 4
    Heat shock 70-kDa protein cognate 4
    Heat shock cognate 4
    Heat shock protein 70
    heat shock cognate 4
    heat shock cognate 70
    heat shock cognate protein 4
    heat shock cognate protein 70-4
    heat shock protein cognate 4
    heat-shock protein 70
    hsp cognate 4
    Secondary FlyBase IDs
    • FBgn0001219
    • FBgn0010802
    • FBgn0011400
    • FBgn0024549
    • FBgn0062154
    • FBgn0066308
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (272)