FlyBase Gene Report: Dmel\Sec6

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Gene: Dmel\Sec6

General Information

Symbol

Dmel\Sec6

Species

D. melanogaster

Name

Secretory 6

Annotation Symbol

CG5341

Feature Type

protein_coding_gene

FlyBase ID

FBgn0266671

Gene Model Status

Stock Availability

9 publicly available

Gene Summary

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. (UniProt, Q9V8K2)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:18,625,249..18,627,718 [+]

Recombination map

2-86

RefSeq locus

NT_033778 REGION:18625249..18627718

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (20 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables SNARE binding

inferred from sequence or structural similarity with SGD:S000001330

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000480155

(GO Reference Genome Project, 2011-)

Biological Process (14 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in border follicle cell migration

inferred from mutant phenotype

(Assaker et al., 2010)

involved_in endocytic recycling

inferred from mutant phenotype

(Langevin et al., 2005)

involved_in exocytosis

inferred from mutant phenotype

(Beronja et al., 2005)

involved_in follicle cell of egg chamber development

inferred from mutant phenotype

(Jia et al., 2015)

involved_in Golgi to plasma membrane protein transport

inferred from mutant phenotype

(Murthy et al., 2005)

involved_in plasma membrane organization

inferred from mutant phenotype

(Beronja et al., 2005)

involved_in protein localization to cell cortex

inferred from mutant phenotype

(Langevin et al., 2005)

involved_in protein localization to plasma membrane

inferred from mutant phenotype

(Beronja et al., 2005, Langevin et al., 2005)

involved_in rhabdomere membrane biogenesis

inferred from mutant phenotype

(Beronja et al., 2005)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in exocyst localization

inferred from biological aspect of ancestor with PANTHER:PTN000480155

(GO Reference Genome Project, 2011-)

involved_in exocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000480155

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR010326

(InterPro Project Members, 2004-)

involved_in neurotransmitter secretion

non-traceable author statement

(Lloyd et al., 2000)

involved_in synaptic vesicle docking

non-traceable author statement

(Lloyd et al., 2000)

involved_in synaptic vesicle targeting

non-traceable author statement

(Lloyd et al., 2000)

involved_in vesicle-mediated transport

inferred from sequence or structural similarity

(Littleton, 2000)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Fabian et al., 2010, Beronja et al., 2005)

part_of exocyst

inferred from direct assay

(Beronja et al., 2005, Sommer et al., 2005)

located_in membrane

inferred from direct assay

(Beronja et al., 2005)

located_in rhabdomere

inferred from direct assay

(Beronja et al., 2005)

located_in zonula adherens

inferred from direct assay

(Beronja et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of exocyst

inferred from sequence or structural similarity

(Littleton, 2000)

inferred from electronic annotation with InterPro:IPR010326

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000480155

(GO Reference Genome Project, 2011-)

non-traceable author statement

(Lloyd et al., 2000)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the SEC6 family. (Q9V8K2)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

EXOCYST -: The exocyst complex is composed of eight proteins. It is involved tethering secretory vesicles to the plasma membrane and may regulate other aspects of intracellular trafficking such as SNARE assembly, cytokinesis and endocytic recycling. The exocyst complex is a member of the Complex Associated with Tethering Containing Helical Rods (CATCHR) family. (Adapted from FBrf0219543).

Protein Function (UniProtKB)

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

(UniProt, Q9V8K2)

Summary (Interactive Fly)

component of the exocyst which tethers secretory vesicles to specific plasma membrane sites - a Rab11 effector that facilitates protein transport to the apical rhabdomere in Drosophila photoreceptor cells - regulates E-Cadherin trafficking from recycling endosomes to the plasma membrane

Interactive Fly

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\Sec6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V8K2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.44

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0086709

2405

738

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0085888

86.7

738

6.90

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

The exocyst complex is composed of Sec3/Exoc1, Sec5/Exoc2, Sec6/Exoc3, Sec8/Exoc4, Sec10/Exoc5, Sec15/Exoc6, Exo70/Exoc7 and Exo84/Exoc8.

(UniProt, Q9V8K2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Q9V8K2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sec6 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sec6_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.04

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

garland cell primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval salivary gland

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis

spermatid cyst | restricted

(Fabian et al., 2010)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Sec6 protein is localized to the growing end of the spermatid cyst

(Fabian et al., 2010)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Fabian et al., 2010, Beronja et al., 2005)

part_of exocyst

inferred from direct assay

(Beronja et al., 2005, Sommer et al., 2005)

located_in membrane

inferred from direct assay

(Beronja et al., 2005)

located_in rhabdomere

inferred from direct assay

(Beronja et al., 2005)

located_in zonula adherens

inferred from direct assay

(Beronja et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sec6 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sec6

View exonic expression by tissue for Dmel\Sec6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266671

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37142/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266671

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034367

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266671

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5341

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sec6

Transgenic constructs containing regulatory region of Sec6

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

(Murthy et al., 2005)

(Ryder, 2004.4.26)

(Beronja et al., 2005)

Gene partially disrupted in

(Juhász et al., 2007)

Df(2R)Sec6¹⁷⁵

(Beronja et al., 2005)

Df(2R)Sec6^Ex15

(Murthy et al., 2005)

Df(2R)Sec6^Ex228

(Murthy et al., 2005)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^grh.D4

Sec6^JF02623

lethal, with Scer\GAL4^pnr-MD237

Sec6^GD11616

lethal (with Df(2R)PC4)

Sec6^Ex228

lethal | recessive

Sec6^Ex228

lethal - all die before end of larval stage

lethal - all die before end of larval stage (with Df(2R)Ex212)

Sec6^Ex15

lethal - all die before end of larval stage (with Df(2R)PC4)

Sec6^Ex15

lethal - all die before end of larval stage | recessive

Sec6^Ex15

partially lethal - majority die | adult stage, with Scer\GAL4^pnr-MD237

Sec6^GD11616

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Sec6^GD11616

viable, with Scer\GAL4^tin.CΔ4

Sec6^GD11616

Sterility

Allele

female sterile germline-dependent | germline clone

Other Phenotypes

Allele

abnormal developmental rate | larval stage, with Scer\GAL4^phtm.PO

abnormal developmental rate | larval stage | recessive

abnormal flight, with Scer\GAL4^Mef2.PR

Sec6^GD11616

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav.PU

Sec6^JF02623

abnormal neuroanatomy | larval stage | somatic clone

Sec6²⁰

abnormal neuroanatomy | somatic clone | third instar larval stage

Sec6^Ex15

abnormal size | adult stage | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

abnormal size | adult stage | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

abnormal size | adult stage | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

abnormal size | adult stage | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

abnormal size | pupal stage | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

abnormal size | pupal stage | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

abnormal size | pupal stage | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

abnormal size | pupal stage | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

decreased body size | larval stage

Sec6^Ex15

decreased body size | larval stage, with Scer\GAL4^grh.D4

Sec6^JF02623

decreased body size | larval stage (with Df(2R)Ex212)

Sec6^Ex15

decreased body size | larval stage (with Df(2R)PC4)

Sec6^Ex15

some die during larval stage

some die during larval stage, with Scer\GAL4^phtm.PO

some die during larval stage (with Df(2R)Ex212)

Sec6^Ex15

some die during larval stage (with Df(2R)PC4)

Sec6^Ex15

some die during larval stage | recessive

Sec6^Ex15

visible, with Scer\GAL4^pnr-MD237

Sec6^GD11616

visible | adult stage | somatic clone

visible | adult stage | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

visible | adult stage | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

visible | adult stage | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

visible | adult stage | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

visible | larval stage, with Scer\GAL4^grh.D4

Sec6^JF02623

Phenotype manifest in

Allele

border follicle cell, with Scer\GAL4^c306

Sec6^VDRC.cUa

cornea | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

cornea | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

cornea | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

cornea | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

cytoplasmic vesicle & eye photoreceptor cell | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

cytoplasmic vesicle & eye photoreceptor cell | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

cytoplasmic vesicle & eye photoreceptor cell | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

cytoplasmic vesicle & eye photoreceptor cell | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

egg chamber | germline clone | oogenesis stage S6

egg chamber | germline clone | oogenesis stage S7

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^elav.PU

Sec6^JF02623

embryonic/larval posterior spiracle | larval stage, with Scer\GAL4^grh.D4

Sec6^JF02623

embryonic/larval tracheal dorsal trunk | first instar larval stage, with Scer\GAL4^grh.D4

Sec6^JF02623

endoplasmic reticulum & eye photoreceptor cell | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

endoplasmic reticulum & eye photoreceptor cell | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

endoplasmic reticulum & eye photoreceptor cell | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

endoplasmic reticulum & eye photoreceptor cell | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

eye | somatic clone

eye | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

eye | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

eye | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

eye | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

eye disc | somatic clone, with Scer\GAL4^Act.PU

Sec6^GD11616

eye photoreceptor cell | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

eye photoreceptor cell | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

eye photoreceptor cell | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

eye photoreceptor cell | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

female germline cell | germline clone

Sec6^Ex15

interommatidial bristle | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

interommatidial bristle | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

interommatidial bristle | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

interommatidial bristle | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

larval multidendritic class IV neuron | larval stage | somatic clone

Sec6²⁰

larval multidendritic class IV neuron | somatic clone | third instar larval stage

Sec6^Ex15

mesonotum, with Scer\GAL4^pnr-MD237

Sec6^GD11616

microvillus & eye photoreceptor cell | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

microvillus & eye photoreceptor cell | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

microvillus & eye photoreceptor cell | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

microvillus & eye photoreceptor cell | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

NMJ bouton | increased number | larval stage, with Scer\GAL4^elav.PU

Sec6^JF02623

nurse cell ring canal | germline clone

Sec6^Ex15

nurse cell ring canal | germline clone | oogenesis stage S6

nurse cell ring canal | germline clone | oogenesis stage S7

oocyte | germline clone

Sec6^Ex15

pigment cell | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

pigment cell | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

pigment cell | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

pigment cell | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

retina | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

retina | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

retina | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

retina | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

rhabdomere | somatic clone, with Scer\GAL4^ey.PH, Sec6²⁰

Sec6^UASp.cBa

rhabdomere | somatic clone, with Scer\GAL4^ey.PH, Sec6^UASp.cBa

Sec6²⁰

rhabdomere | somatic clone (with Sec6²⁰), with Scer\GAL4^ey.PH

Sec6^UASp.cBa

rhabdomere | somatic clone (with Sec6^UASp.cBa), with Scer\GAL4^ey.PH

Sec6²⁰

tormogen cell, with Scer\GAL4^pnr-MD237

Sec6^GD11616

wing disc, with Scer\GAL4^dpp.blk1, Scer\GAL80^ts.αTub84B

Sec6^GD11616

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

14 of 14

Yes

Yes

6 of 14

No

Yes

5 of 14

No

Yes

5 of 14

No

Yes

4 of 14

No

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

14 of 14

Yes

Yes

6 of 14

No

Yes

5 of 14

No

Yes

5 of 14

No

Yes

2 of 14

No

Yes

Mus musculus (laboratory mouse) (5)

14 of 14

Yes

Yes

6 of 14

No

Yes

5 of 14

No

Yes

5 of 14

No

Yes

3 of 14

No

Yes

Xenopus tropicalis (Western clawed frog) (6)

8 of 13

Yes

Yes

4 of 13

No

Yes

3 of 13

No

Yes

2 of 13

No

Yes

1 of 13

No

Yes

1 of 13

No

No

Danio rerio (Zebrafish) (9)

14 of 14

Yes

Yes

6 of 14

No

Yes

5 of 14

No

Yes

5 of 14

No

Yes

4 of 14

No

Yes

4 of 14

No

Yes

Drer\si:dkey-45k15.1

4 of 14

No

Yes

1 of 14

No

Yes

Drer\si:dkey-196h17.9

1 of 14

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

14 of 14

Yes

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP009058

12 of 12

Yes

Yes

Arabidopsis thaliana (thale-cress) (1)

12 of 13

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

10 of 13

Yes

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

10 of 12

Yes

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sec6. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS

(Chabu and Xu, 2014)

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

exacerbates muscular dystrophy

modeled by Dg^O86

(Yatsenko et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EXOC3; exocyst complex component 3

14 of 14

EXOC3L1; exocyst complex component 3 like 1

6 of 14

EXOC3L4; exocyst complex component 3 like 4

5 of 14

TNFAIP2; TNF alpha induced protein 2

5 of 14

EXOC3L2; exocyst complex component 3 like 2

4 of 14

BRAIN MALFORMATION RENAL SYNDROME; BMRS

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

On

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

cosedimentation through density gradient, western blot

(Sommer et al., 2005)

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

anti bait coimmunoprecipitation, western blot

(Peng et al., 2015)

cosedimentation through density gradient, western blot

(Sommer et al., 2005)

anti bait coimmunoprecipitation, western blot, cosedimentation through density gradient

(Beronja et al., 2005, Sommer et al., 2005)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Atienza-Manuel et al., 2021)

enhanceable

(Blankenship et al., 2007)

suppressible

(Chabu and Xu, 2014)

External Data

Subunit Structure (UniProtKB)

The exocyst complex is composed of Sec3/Exoc1, Sec5/Exoc2, Sec6/Exoc3, Sec8/Exoc4, Sec10/Exoc5, Sec15/Exoc6, Exo70/Exoc7 and Exo84/Exoc8.

(UniProt, Q9V8K2 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266671

MIST (genetic) - An integrated Molecular Interaction Database

37142

MIST (protein-protein) - An integrated Molecular Interaction Database

37142

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-86

Cytogenetic map

Sequence location

2R:18,625,249..18,627,718 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55E2-55E2

Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)03709⁰³⁷⁰⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.

(Lloyd et al., 2000)

55E-55F

(Lloyd et al., 2000)

Experimentally Determined Recombination Data

Location

2-86

(FlyBase, 2016)

2-91.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

27314

y¹ v¹; P{TRiP.JF02623}attP2

54582

y¹ w^*; PBac{Sec6^t2.8}VK00005

17635

y¹ w^67c23; P{EPgy2}Sec6^EY10058

15126

y¹; P{SUPor-P}Sec6^KG08199/CyO; ry⁵⁰⁶

HMJ21305

y¹ v¹; P{TRiP.HMJ21305}attP40/CyO

v105836

P{KK102544}VIE-260B

v318479

PBac{fTRG10004.sfGFP-FT}VK00033

v22077

w¹¹¹⁸; P{GD11616}v22077

v318405

PBac{fTRG10195.sfGFP-nlsTTSTF}VK00033

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (79)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD24661

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266671

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Beronja et al., 2005)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

S2 cells treated with dsRNA directed against sec6 show normal binding and internalization of E.coli and S.aureus in a phagocytosis assay.

(Stuart et al., 2007)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: Sec6 sec6

(FlyBase, 1992-)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Sec6 sec6

(FlyBase, 2009-)

Source for identity of: sec6 CG5341

(Verstreken and Bellen, 2000.9.4)

Nomenclature comments

Etymology

Named "Sec6" after the S. cerevisiae ortholog.

(FlyBase, 2009-)

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG5341

(Laffafian and Tepass, 2019, Hosono et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Beronja et al., 2005, Beronja and Tepass, 2003)

Dsec6

(Beronja and Tepass, 2003)

Sec6p

(Littleton, 2000)

dsec6

(Lloyd et al., 2000)

sec 6

(Mazumdar and Mazumdar, 2002)

Name Synonyms

Sec6 ortholog

Secretory 6

Secondary FlyBase IDs

FBgn0266611
FBgn0034367

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37142

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V8K2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V8K2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V8K2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266671

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37142/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266671

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266671

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5341

MARRVEL_MODEL - MARRVEL (model organism gene)

37142

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266671

DroID - A comprehensive database of gene and protein interactions.

FBgn0266671

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sec6_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034367

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0266671

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5341

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0266671

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006514

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

37142

MIST (protein-protein) - An integrated Molecular Interaction Database

37142

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (95)