FlyBase Gene Report: Dmel\CG32486

General Information

Symbol

Dmel\CG32486

Species

D. melanogaster

Name

Annotation Symbol

CG32486

Feature Type

protein_coding_gene

FlyBase ID

FBgn0266918

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

63A5-63A6

Sequence location

3L:3,055,908..3,070,839 [-]

Recombination map

3-5

RefSeq locus

NT_037436 REGION:3055908..3070839

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR001293

(InterPro Project Members, 2004-)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001128604

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the ZFTRAF1 family. (Q9VZV5)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG32486 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VZV5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG32486-RD

FBtr0073004

NM_139508

2356

412

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG32486-PD

FBpp0072874

45.4

412

7.65

Q9VZV5

NP_647765

AAF47711

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VZV5

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG32486 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG32486_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.24

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

amnioserosa

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

anterior midgut primordium

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG32486 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG32486

View exonic expression by tissue for Dmel\CG32486

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266918

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38367/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266918

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052486

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266918

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32486

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG32486

Transgenic constructs containing regulatory region of CG32486

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

CG32486^CB03414

lethal | recessive

CG32486^SH095

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

CG32486^GD10912

partially lethal - majority die, with Scer\GAL4^pnr-MD237

CG32486^GD10912

viable

CG32486^CC00904

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG32486^GD10912

viable, with Scer\GAL4^tin.CΔ4

CG32486^GD10912

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

CG32486^GD10912

visible, with Scer\GAL4^pnr-MD237

CG32486^GD10912

Phenotype manifest in

Allele

mesonotum, with Scer\GAL4^pnr-MD237

CG32486^GD10912

trichogen cell, with Scer\GAL4^pnr-MD237

CG32486^GD10912

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (11)

Hsap\CYHR1

14 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Cyhr1

12 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Rnor\Rnf41

1 of 14

Rnor\Rnf151

1 of 14

Rnor\Rnf166

1 of 14

Yes

Rnor\Traf3

1 of 14

Rnor\Traf5

1 of 14

Rnor\Trim50

1 of 14

Yes

Mus musculus (laboratory mouse) (10)

Mmus\Cyhr1

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Traf5

1 of 14

Mmus\Trim50

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\LOC101735269

7 of 13

Yes

Xtro\pdzrn3

1 of 13

Xtro\rnf41

1 of 13

Xtro\siah1

1 of 13

Danio rerio (Zebrafish) (8)

Drer\cyhr1

14 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\siah-1

2 of 14

Yes

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP002264

11 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (17)

Atha\AT1G66610

3 of 13

Yes

Atha\AT1G66620

3 of 13

Yes

Atha\AT1G66630

3 of 13

Yes

Atha\AT1G66650

3 of 13

Yes

Atha\AT1G66660

3 of 13

Yes

Atha\AT5G37870

3 of 13

Yes

Atha\AT5G37890

3 of 13

Yes

Atha\AT5G37900

3 of 13

Yes

Atha\AT5G37910

3 of 13

Yes

Atha\AT5G37930

3 of 13

Yes

Atha\AT5G62800

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG32486. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ZFTRAF1; zinc finger TRAF-type containing 1

14 of 14

616635

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG32486 - Gs1

experimental knowledge based

(Guruharsha et al., 2011)

CG32486 - Hibch

experimental knowledge based

(Guruharsha et al., 2011)

CG32486 - RabX1

experimental knowledge based

(Guruharsha et al., 2011)

CG32486 - Ubc4

experimental knowledge based

(Guruharsha et al., 2011)

CG32486 - lwr

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cindr

enhanceable

CG32486

(Ketosugbo et al., 2017)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266918

MIST (protein-protein) - An integrated Molecular Interaction Database

38367

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-5

Cytogenetic map

63A5-63A6

Sequence location

3L:3,055,908..3,070,839 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63A5-63A6

Limits computationally determined from genome sequence between P{PZ}l(3)06803⁰⁶⁸⁰³ and P{PZ}kst⁰¹³¹⁸; Left limit from in situ hybridisation (FBrf0157097) Right limit from in situ hybridisation (FBrf0157097)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

63A4-63A6

(Oh et al., 2003)

Experimentally Determined Recombination Data

Location

3-5

(FlyBase, 2016)

3-6.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

51545

w^*; P{PTT-GC}CG32486^CC00904

37654

y¹ w^*; Mi{MIC}CG32486^MI04382

16033

y¹ w¹¹¹⁸; PBac{5HPw⁺}CG32486^A077

35465

y¹ sc^* v¹ sev²¹; P{TRiP.GL00391}attP2

29467

w^*; P{lacW}CG32486^SH095 P{FRT(w^hs)}2A/TM3, Sb¹

77745

y¹ w^*; Mi{Trojan-GAL4.0}CG32486^{MI04382-TG4.0}/TM3, Sb¹ Ser¹

NIG-Fly

GL00391

y¹ sc^* v¹ sev²¹; P{TRiP.GL00391}attP2

VDRC

v41907

w¹¹¹⁸; P{GD10912}v41907

v41908

w¹¹¹⁸; P{GD10912}v41908

Genomic Clones (31)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (69)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD47625

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266918

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

1 allele of l(3)SH095 recovered in a P-insertion screen.

(Oh et al., 2003)

Area matching Drosophila EST AA440953.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG14954 CG16745

(Misra, 2001.8.10)

Source for merge of: CG32486 anon-WO0118547.267

(FlyBase, 1996-)

Source for merge of: CG32486 l(3)SH095

(FlyBase, 1996-)

Additional comments

Annotations CG14954, CG16745 merged as CG32486 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG32486 anon-WO0118547.267 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG14954

CG16745

(Misra, 2001.8.10)

CG32486

(Zhou et al., 2024, Hu et al., 2017.6.13, Ketosugbo et al., 2017, Gene Disruption Project members, 2016-, Ashwal-Fluss et al., 2014, Luck et al., 2014, Olovnikov et al., 2013, Du et al., 2011, Ni et al., 2011.7.19, Li et al., 2010, Hurlbut et al., 2009, Buszczak et al., 2007)

anon-WO0118547.267

l(2)SH3 095

(Oh et al., 2002.3.26)

l(3)SH095

Name Synonyms

lethal (3) SH095

Secondary FlyBase IDs

FBgn0052486
FBgn0035394
FBgn0035395
FBgn0062132
FBgn0065142

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 34 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38367

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VZV5

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VZV5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VZV5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266918

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38367/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266918

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266918

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32486

MARRVEL_MODEL - MARRVEL (model organism gene)

38367

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266918

DroID - A comprehensive database of gene and protein interactions.

FBgn0266918

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG32486_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052486

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0266918

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32486

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014541

MIST (protein-protein) - An integrated Molecular Interaction Database

38367

References (47)

The following external sites may use different assemblies or annotations than FlyBase.

-0.24

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