roe, roughened eye, tot, P5.3, tod
transcription factor - Kruppel zinc finger family member - required for proper imaginal disc development - functions as an olfactory neuron specification factor required for diversification of multiple developmental lineages - mutants are defective for multiple distal structures in the adult, including smaller legs, wings and eyes
Gene model reviewed during 5.49
Gene model reviewed during 5.44
Stop-codon suppression (UAG) postulated; FBrf0216884.
gene_with_stop_codon_read_through ; SO:0000697
Due to an oversight in the implementation of gene annotation merges, the symbols for some of the transcripts and polypeptides of this gene were changed to non-unique designations in r5.8. Although there were no conflicts within a given release, the same symbols were used for different isoforms in different releases. The affected gene product symbols have now been changed to symbols not used previously in the database; see individual transcript reports for details.
Gene model reviewed during 5.43
None of the polypeptides share 100% sequence identity.
Interacts with nab; which acts as a corepressor.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\rn using the Feature Mapper tool.
rn expression is first observed just posterior to the broad stripe of ato expression that is anterior to and within the morphogenetic furrow. rn is initially seen within the first column of nascent R8 nuclei as well as in nuclei surrounding these R8 cells. It then appears to be expressed only in the nuclei immediately surrounding the second column of R8 cells and is no longer expressed near the third column of R8 cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\rn in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Annotations CG32466 and CG32467 merged as CG42277 in release 5.8 of the genome annotation.
Annotation CG14601 split into CG32467 and part of CG32466 (rn) in release 3 of the genome annotation. Release 3 annotation CG32466 contains sequences corresponding to release 2 annotations CG10040, CG14603, CG14600 and part of CG14601.
Source for merge of rn CG10040 CG14600 CG14601 was sequence comparison ( date:020121 ).
"rn" and "roe" may correspond to a single gene, since mutations which affect "rn" often also affect "roe", or they may be two closely linked genes.
"rn" shares an intimate relationship with "roe", mutagenesis of one locus often results in simultaneous mutagenesis of the other.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
The rn locus encodes two different transcripts ("rotund" and "roughened eye") which are expressed in non-overlapping domains in the larval imaginal discs. The "rotund" and "roughened eye" proteins share a C-terminus but have unique N-termini. Each transcript can rescue only its corresponding mutation and they show negative effects when expressed in each other's domain of expression. The "rotund" and "roughened eye" phenotypes are genetically separable. In direction of increasing cytology: rn- RacGAP84C+ rn-
"rn" and "roe" functions of the rn gene are encoded by two transcripts which use different origins of transcription but share the same 3' exons. The proteins encoded by the two transcripts are functionally different; "UAS-rn" constructs can rescue the "rn" phenotype but not "roe", whereas "UAS-roe" constructs rescue "roe" phenotypes but not "rn".
Expression of rn transcription in rn mutants suggests that the m5.3 transcript encodes rn morphogenetic function in the imaginal discs. Expression partitions all major imaginal discs into domains along the proximo-distal axis: two in the wing and haltere discs, and at least three in the leg and antennae discs. The m5.3 transcript is sex-regulated in the genital disc and is only expressed in the female, suggesting that this disc is organised similar to other imaginal discs. It is not yet clear whether rn function is required for establishment or maintainance of patterning in the proximo-distal axis.
All alleles are viable as homozygotes. Both males and females are sterile; sterility is not germ line- dependent. Two transcripts from the rotund region of Drosophila show similar positional specificities in imaginal disc tissues. Adult defects are restricted to homologous distal parts of the appendages, i.e., the antennae, legs, wings, halteres and proboscis. In the antenna the basal capsule is missing and the third antennal segment is reduced in size; all other antennal and eye disc derivatives are normal. In all three pairs of legs abnormalities caused by the lack of rn+ product are localized specifically in the tarsus; instead of five individual tarsal segments, a single tarsus-like segment differentiates. The distal claw and proximal leg articulations (tibia, femur, trochanter, and coxa) are unaffected. Incomplete and duplicated joints seen at the presumptive positions of the tarsus 1-2 and 4-5 joints; intermediate joints virtually absent. Of the wing disc derivatives, the medial and distal costa are fused and the corresponding region in the posterior wing, the alula, is smaller, making the wing as a whole appear shorter; distal wing and mesonotum are formed as in wild type. Vein L5 and to a lesser extent L2 interrupted (rn3). Specific and localized deficiencies of labial and haltere disc derivatives are also evident. Genital and abdominal patterns are indistinguishable from wild-type patterns; however, seminal receptacles shorter than in wild-type females. Examination of the imaginal discs from third instar rn larvae shows localized cell death in regions determined from fate maps to give rise to distal appendage parts.
The abnormal rn phenotype is due to a developmental defect: late occurring defects. Adult eye is rough and slightly reduced, eye disc pattern appears normal.