FlyBase Gene Report: Dmel\kl-3

General Information

Symbol

Dmel\kl-3

Species

D. melanogaster

Name

male fertility factor kl3

Annotation Symbol

CG45785

Feature Type

protein_coding_gene

FlyBase ID

FBgn0267432

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

male fertility factor kl3 (kl-3) encodes a dynein heavy chain that is a component of a microtubule motor complex. This motor activity is essential only for spermatogenesis. Lack of kl-3 results in loss of the axonemal out dynein arms in the spermatid tail. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

kl3, Lms3

Key Links

Genomic Location

Cytogenetic map

h8-h8

Sequence location

Y:336,381..563,165 [+]

Recombination map

Y-

RefSeq locus

NC_024512 REGION:336381..563165

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI

UCSC

Ensembl

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR035699

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR003593

(InterPro Project Members, 2004-)

enables dynein intermediate chain binding

inferred from electronic annotation with InterPro:IPR026983

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000743491

(GO Reference Genome Project, 2011-)

enables dynein light intermediate chain binding

inferred from biological aspect of ancestor with PANTHER:PTN000743491

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR026983

(InterPro Project Members, 2004-)

enables minus-end-directed microtubule motor activity

inferred from electronic annotation with InterPro:IPR004273

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P45444

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000743491

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in flagellated sperm motility

inferred from expression pattern

(Zur Lage et al., 2021)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in cilium movement involved in cell motility

inferred from biological aspect of ancestor with PANTHER:PTN000743491

(GO Reference Genome Project, 2011-)

involved_in cilium movement

inferred from sequence or structural similarity with UniProtKB:Q8TE73

(Gene Ontology Curators, 2002-)

involved_in microtubule-based movement

inferred from electronic annotation with InterPro:IPR004273, InterPro:IPR026983

(InterPro Project Members, 2004-)

involved_in outer dynein arm assembly

inferred from biological aspect of ancestor with PANTHER:PTN002472868

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of axonemal dynein complex

inferred from mutant phenotype

(Goldstein et al., 1982)

is_active_in sperm flagellum

inferred from direct assay

(Zur Lage et al., 2021)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of dynein complex

inferred from electronic annotation with InterPro:IPR004273, InterPro:IPR026983

(InterPro Project Members, 2004-)

part_of outer dynein arm

inferred from sequence or structural similarity with UniProtKB:Q8TE73

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN002472868

(GO Reference Genome Project, 2011-)

is_active_in sperm flagellum

inferred from biological aspect of ancestor with PANTHER:PTN008503977

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

AXONEMAL OUTER ARM DYNEIN HEAVY CHAINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Snapshot

Gene Group (FlyBase)

AXONEMAL OUTER ARM DYNEIN HEAVY CHAINS -: Dynein heavy chains are minus end-directed microtubule motor proteins. Axonemal dynein complexes interact with adjacent microtubules in the axoneme. Coordinated binding and release of the axonemal dyneins slide the microtubules relative to each other causing the axoneme to bend driving ciliary and flagellar motion. Axonemal outer arm dynein heavy chains are the microtubule-binding motor protein of the dynein outer arm. (Adapted from FBrf0230280).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

kl-3

Males mutant for kl-3 fail to assemble the outer dynein arms associated with the peripheral nine microtubule doublets of the sperm-tail axoneme [Hardy, Tokuyasu, and Lindsley, 1981, Chromosoma 83: 593-617 (fig.)]. Such males also fail to produce a kl-3-specific 300-325 kilodalton sperm polypeptide presumed to be a component of the outer dynein arms (Goldstein, Hardy, and Lindsley, 1982, Proc. Nat. Acad. Sci. USA 79: 7404-09). Such mutants display an exceedingly low level of fertility at 25 but not at 18 immediately upon eclosion, but not thereafter (Kennison, 1983, Genetics 103: 219-34). Deficiencies for kl-3 are completely sterile, and in addition to the above mutant phenotype, they fail to elaborate the loops (ribbon-like structure) observed in the primary spermatocyte nuclei of normal males by light microscopy and the reticular material ordinarily observed by electron microscopy in spermatocyte nuclei (Hardy et al., 1981). The kl-3 loops are visible in living spermatocytes and in fixed cells stained with the protein-specific dye CBB; these loops are also demonstrated by the polyclonal antibody Sph-155 but do not react with monoclonal antibody S5 (Bonaccorsi et al., 1988).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\kl-3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A8Y5B7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 6.01

Gene model reviewed during 6.02

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

kl-3-RC

FBtr0346771

NM_001111012

13882

4593

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

kl-3-PC

FBpp0312366

532.0

4593

5.89

A8Y5B7

NP_001104482

EDP28010

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kl-3 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

mutational analysis

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

spermatogonium

Comment: data from FlyAtlas

(Zur Lage et al., 2019)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

spermatogonium

(Zur Lage et al., 2019)

Additional Descriptive Data

As part of a survey of ciliary motility gene homologs in Drosophila, expression of kl-3 was assayed in motile ciliated cell types. Expression was observed in sperm (testis) but not chordotonal neurons.

(Zur Lage et al., 2019)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of axonemal dynein complex

inferred from mutant phenotype

(Goldstein et al., 1982)

is_active_in sperm flagellum

inferred from direct assay

(Zur Lage et al., 2021)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\kl-3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\kl-3

View exonic expression by tissue for Dmel\kl-3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

26067053/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267432

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001314

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267432

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 24 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of kl-3

Transgenic constructs containing regulatory region of kl-3

Aberrations (Deficiencies and Duplications) ( 73 )

Inferred from experimentation ( 73 )

Gene disrupted in

Df(YL)A10

(Hazelrigg et al., 1982)

Df(YL)A11

(Hazelrigg et al., 1982)

Df(YL)A17

(Hazelrigg et al., 1982)

Df(YL)A32

(Hazelrigg et al., 1982)

Df(YL)A39

(Hazelrigg et al., 1982)

Df(YL)A49

(Hazelrigg et al., 1982)

Df(YL)B4

(Hazelrigg et al., 1982)

Df(YL)B12

(Hazelrigg et al., 1982)

Df(YL)B14

(Hazelrigg et al., 1982)

Df(YL)G19

(Hardy et al., 1984)

Df(YL)G20

(Hardy et al., 1984)

Df(YL)G21

(Hardy et al., 1984)

Df(YL)G22

(Hardy et al., 1984)

Df(YL)G23

(Hardy et al., 1984)

Df(YL)G24

(Hardy et al., 1984)

Df(YL)H121

(Bonaccorsi and Lohe, 1991)

Df(YL)S7

(Gatti and Pimpinelli, 1983)

Df(YL)S8

(Gatti and Pimpinelli, 1983)

Df(YL)S9

(Gatti and Pimpinelli, 1983)

Df(YL)S10

(Gatti and Pimpinelli, 1983)

Df(YL)S11

(Gatti and Pimpinelli, 1983)

Df(YL)S13

(Gatti and Pimpinelli, 1983)

Df(YL)S14

(Gatti and Pimpinelli, 1983)

In(YL)B2

(Gatti and Pimpinelli, 1983)

In(YL)S15

(Gatti and Pimpinelli, 1983)

In(YL)S16L

(Gatti and Pimpinelli, 1983)

(Bonaccorsi and Lohe, 1991)

T(1;Y)V57

T(Y;2)G74

(Bonaccorsi and Lohe, 1991, Gatti and Pimpinelli, 1983)

T(Y;2)P57

(Bonaccorsi and Lohe, 1991, Gatti and Pimpinelli, 1983)

T(Y;3)D107

(Gatti and Pimpinelli, 1983)

T(Y;3)S20

(Gatti and Pimpinelli, 1983)

Ts(1Lt;YSt)E15

(Zhang and Stankiewicz, 1998)

Ts(1Lt;YSt)F12

(Zhang and Stankiewicz, 1998)

Ts(1Lt;YSt)T13

(Bonaccorsi et al., 1990)

Ts(1Lt;YSt)W27

(Zhang and Stankiewicz, 1998)

Ts(1Rt;YLt)V24

(Bonaccorsi et al., 1990)

Ts(1Rt;YSt)V8

(Bonaccorsi et al., 1990)

Ts(1Rt;YSt)W19

(Bonaccorsi et al., 1990)

Ts(YSt;4Rt)B15

(Hazelrigg et al., 1982)

Gene not disrupted in

Df(YL)S5

(Gatti and Pimpinelli, 1983)

Df(YL)S6

(Gatti and Pimpinelli, 1983)

Df(YS)A6

(Hazelrigg et al., 1982)

Df(YS)B13

(Hazelrigg et al., 1982)

In(YL)B3

(Gatti and Pimpinelli, 1983)

In(YLS)A33

(Hazelrigg et al., 1982)

In(YLS)B1

(Gatti and Pimpinelli, 1983)

In(YLS)S18

(Gatti and Pimpinelli, 1983)

T(1;Y)V24

(Gatti and Pimpinelli, 1983)

T(Y;2)A169

(Gatti and Pimpinelli, 1983)

T(Y;2)B3

(Hazelrigg et al., 1982)

T(Y;2)B13

(Hazelrigg et al., 1982)

T(Y;2)B92

(Gatti and Pimpinelli, 1983)

T(Y;2)B242

(Gatti and Pimpinelli, 1983)

T(Y;2)J146

(Gatti and Pimpinelli, 1983)

T(Y;2)R155

(Gatti and Pimpinelli, 1983)

T(Y;3)A23

(Gatti and Pimpinelli, 1983)

T(Y;3)A34

(Hazelrigg et al., 1982)

T(Y;3)A112

(Gatti and Pimpinelli, 1983)

T(Y;3)B115

(Gatti and Pimpinelli, 1983)

T(Y;3)B223

(Gatti and Pimpinelli, 1983)

T(Y;3)B229

(Gatti and Pimpinelli, 1983)

T(Y;3)S21

(Gatti and Pimpinelli, 1983)

Ts(1Lt;YLt)V8

(Zhang and Stankiewicz, 1998)

Ts(1Lt;YLt)W19

(Zhang and Stankiewicz, 1998)

Ts(1Lt;YSt)V24

(Zhang and Stankiewicz, 1998)

Ts(YLt;3Lt)W27

(Bonaccorsi et al., 1990)

Ts(YLt;3Rt)A34

(Hazelrigg et al., 1982)

Ts(YLt;4Lt)B15

(Hazelrigg et al., 1982)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^Mef2.PR

kl-3^GD9429

viable, with Scer\GAL4^pnr-MD237

kl-3^GD9429

Sterility

Allele

male sterile

male sterile (with Ab(Y)Y2)

male sterile (with Ab(Y)Y146)

sterile, with Scer\GAL4^VP16.bam

kl-3^KK115924

Other Phenotypes

Allele

decreased fecundity, with Scer\GAL4^VP16.bam

kl-3^HMC03546

Phenotype manifest in

Allele

spermatid

spermatid axoneme

spermatozoon

kl-3^104b

spermatozoon | absent, with Scer\GAL4^VP16.bam

kl-3^HMC03546

sperm individualization complex | spermatogenesis, with Scer\GAL4^VP16.bam

kl-3^HMC03546

testis

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (16)

Hsap\DNAH5

9 of 14

Yes

8 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Dnah5

8 of 14

Yes

Rnor\Dnah8

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (19)

Mmus\Dnah5

9 of 14

Yes

8 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\dnah5

7 of 13

Yes

Xtro\LOC100488102

7 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (14)

Drer\dnah5

8 of 14

Yes

6 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\che-3

3 of 14

Yes

Cele\dhc-1

3 of 14

Yes

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP007675

10 of 12

Yes

4 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\DYN1

3 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\dhc1

4 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:kl-3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (14)

9 of 13

7 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

btv

5 of 13

5 of 13

5 of 13

4 of 13

2 of 13

sac

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

DNAH5; dynein axonemal heavy chain 5

model of primary ciliary dyskinesia 3

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DNAH5; dynein axonemal heavy chain 5

9 of 14

603335

CILIARY DYSKINESIA, PRIMARY, 3; CILD3

primary ciliary dyskinesia 3

DNAH8; dynein axonemal heavy chain 8

8 of 14

603337

SPERMATOGENIC FAILURE 46; SPGF46

spermatogenic failure 46

DNAH10; dynein axonemal heavy chain 10

3 of 14

605884

SPERMATOGENIC FAILURE 56; SPGF56

spermatogenic failure 56

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0267432

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

Class of Gene

nuclear_gene

(Drosophila Heterochromatin Genome Project, 2007)

protein_coding_gene

(Drosophila Heterochromatin Genome Project, 2007)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

Y-

Cytogenetic map

h8-h8

Sequence location

Y:336,381..563,165 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

h8-h8

Left limit from complementation mapping against T(Y;3)S20 (FBrf0039037) Right limit from complementation mapping against T(Y;3)D107 (FBrf0039037)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Maps to the region of kl-3.

(Zhang, 1996.4.28)

h7-h9

(Drosophila Heterochromatin Genome Project, 2004, Zhang et al., 1996)

h7-h9

(determined by in situ hybridisation)

(Zhang and Stankiewicz, 1998)

Experimentally Determined Recombination Data

Location

Y-

(Hackstein and Hochstenbach, 1995)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

93008

w^*; P{U6:3-kl-3.gRNA}attP2

53317

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03546}attP2

2756

T(Y;2)P57, Bar^S, y⁺ kl-3^P57/SM1; C(1)RM, y¹/C(1;Y)1, y¹

NIG-Fly

HMJ22635

y¹ v¹; P{TRiP.HMJ22635}attP40/CyO

VDRC

v109151

P{KK115924}VIE-260B

v32971

w¹¹¹⁸; P{GD9429}v32971

v32972

w¹¹¹⁸; P{GD9429}v32972/CyO

Genomic Clones (3)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Sequence of transcript(s) changed in r6 genomic release relative to r5 release.

(FlyBase Genome Annotators, 2014)

New annotation (CG40992) in release 5.2 of the genome annotation.

(Drosophila Heterochromatin Genome Project, 2007)

New annotation (CG40936) in release 5.2 of the genome annotation.

(Drosophila Heterochromatin Genome Project, 2007)

Transiently named CG40443 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

It is not yet known if "ms(Y)104b" is the same as kl-3.

(Zhang, 1996.4.28)

Primary spermatocyte nuclei exhibit three giant lampbrush-like loops formed by the kl-5, kl-3 and ks-1 Y chromosome fertility factors.

(Pisano et al., 1993)

Males mutant for kl-3 fail to assemble the outer dynein arms associated with the peripheral nine microtubule doublets of the sperm-tail axoneme (Hardy, Tokuyasu, and Lindsley, 1981). Such males also fail to produce a kl-3-specific 300-325 kilodalton sperm polypeptide presumed to be a component of the outer dynein arms (Goldstein, Hardy and Lindsley, 198). Such mutants display an exceedingly low level of fertility at 25^oC but not at 18^oC immediately upon eclosion, but not thereafter (Kennison, 1983). Deficiencies for kl-3 are completely sterile, and in addition to the above mutant phenotype, they fail to elaborate the loops (ribbon-like structure) observed in the primary spermatocyte nuclei of normal males by light microscopy and the reticular material ordinarily observed by electron microscopy in spermatocyte nuclei (Hardy et al., 1981). The kl-3 loops are visible in living spermatocytes and in fixed cells stained with the protein-specific dye CBB; these loops are also demonstrated by the polyclonal antibody Sph-155 but do not react with monoclonal antibody S5 (Bonaccorsi et al., 1988).

Relationship to Other Genes

Source for database merge of

Source for merge of: kl-3 CG17629

(FlyBase, 1996-)

Source for merge of: kl-3 CG40992 CG40936

(FlyBase Genome Annotators, 2014)

Additional comments

Annotations CG40992, CG40936 and CG17629 merged as CG45785 in release 6.02 of the genome annotation. This gene is no longer fragmented in the release 6 genome assembly.

(FlyBase Genome Annotators, 2014)

Source for merge of kl-3 CG17629 was sequence comparison ( date:001108 ).

(FlyBase, 1996-)

Williamson (1972) reported 23 non-complementing and nine complementing alleles induced by EMS.

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

CG17629

(Meadows, 2014.12.12, Yu et al., 2013, Ni et al., 2010.12.1, Drosophila Heterochromatin Genome Project, 2007, Wickstead and Gull, 2007, Goldstein and Gunawardena, 2000)

CG40443

(Giot et al., 2003)

CG40936

(Keleman et al., 2009.8.5, Drosophila Heterochromatin Genome Project, 2007)

CG40992

(National Institute of Genetics Fly Stocks, 2023-, Drosophila Heterochromatin Genome Project, 2007)

CG45785

(FlyBase Genome Annotators, 2014)

DHC4A

(Kollmar, 2015.9.15)

Kl-3

(Siddall and Hime, 2017)

Kl3-Y

(Zanini et al., 2018)

Lms3

(Hazelrigg et al., 1982)

Y104b

(Zhang et al., 1996)

kl-4

(Hackstein et al., 1996)

kl3

(Hafezi et al., 2024, Hafezi et al., 2020, Singh et al., 2014, Zhang et al., 1998, Zhang et al., 1996)

ms(Y)104b

Name Synonyms

male fertility factor kl-3

(Hoskins et al., 2015)

male fertility factor kl3

(Garlovsky et al., 2022, Wasbrough et al., 2010)

γ-dynein heavy chain

(Gvozdev et al., 2005)

Secondary FlyBase IDs

FBgn0085550
FBan0040992
FBgn0085539
FBan0040936
FBgn0016045
FBgn0040039
FBgn0058443
FBan0017629
FBgn0001314

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

26067053

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A8Y5B7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A8Y5B7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

26067053/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267432

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267432

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG45785

MARRVEL_MODEL - MARRVEL (model organism gene)

26067053

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0267432

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001314

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0267432

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034619

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

References (117)

The following external sites may use different assemblies or annotations than FlyBase.

NA

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