FB2020_01, released Feb 12, 2020

Gene: Dmel\kl-3

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General Information
Symbol
Dmel\kl-3
Species
D. melanogaster
Name
male fertility factor kl3
Annotation Symbol
CG45785
Feature Type
protein_coding_gene
FlyBase ID
FBgn0267432
Gene Model Status
Current
Stock Availability
5 publicly available
Enzyme Name (EC)
Adenosinetriphosphatase (3.6.1.3)
Minus-end-directed kinesin ATPase (5.6.1.4)
Gene Snapshot
male fertility factor kl3 (kl-3) encodes a dynein heavy chain that is a component of a microtubule motor complex. This motor activity is essential only for spermatogenesis. Lack of kl-3 results in loss of the axonemal out dynein arms in the spermatid tail. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

kl3, Lms3

Key Links
Genomic Location
Cytogenetic map
h8-h8
Sequence location
Y:336,381..563,165 [+]
Recombination map

Y-

RefSeq locus
NC_024512 REGION:336381..563165
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
ATP binding; ATP-dependent microtubule motor activity, minus-end-directed; ATPase activity, coupled; dynein intermediate chain binding; dynein light intermediate chain binding
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O = ADP + phosphate (3.6.1.3)
ATP + H(2)O + a kinesin associated with a microtubule at position (n) = ADP + phosphate + a kinesin associated with a microtubule at position (n-1, toward the minus end) (5.6.1.4)
Summaries
Gene Group (FlyBase)
AXONEMAL OUTER ARM DYNEIN HEAVY CHAINS -
Dynein heavy chains are minus end-directed microtubule motor proteins. Axonemal dynein complexes interact with adjacent microtubules in the axoneme. Coordinated binding and release of the axonemal dyneins slide the microtubules relative to each other causing the axoneme to bend driving ciliary and flagellar motion. Axonemal outer arm dynein heavy chains are the microtubule-binding motor protein of the dynein outer arm. (Adapted from FBrf0230280).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
kl-3
Males mutant for kl-3 fail to assemble the outer dynein arms associated with the peripheral nine microtubule doublets of the sperm-tail axoneme [Hardy, Tokuyasu, and Lindsley, 1981, Chromosoma 83: 593-617 (fig.)]. Such males also fail to produce a kl-3-specific 300-325 kilodalton sperm polypeptide presumed to be a component of the outer dynein arms (Goldstein, Hardy, and Lindsley, 1982, Proc. Nat. Acad. Sci. USA 79: 7404-09). Such mutants display an exceedingly low level of fertility at 25 but not at 18 immediately upon eclosion, but not thereafter (Kennison, 1983, Genetics 103: 219-34). Deficiencies for kl-3 are completely sterile, and in addition to the above mutant phenotype, they fail to elaborate the loops (ribbon-like structure) observed in the primary spermatocyte nuclei of normal males by light microscopy and the reticular material ordinarily observed by electron microscopy in spermatocyte nuclei (Hardy et al., 1981). The kl-3 loops are visible in living spermatocytes and in fixed cells stained with the protein-specific dye CBB; these loops are also demonstrated by the polyclonal antibody Sph-155 but do not react with monoclonal antibody S5 (Bonaccorsi et al., 1988).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\kl-3 or the JBrowse view of Dmel\kl-3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 6.01

Gene model reviewed during 6.02

Sequence Ontology: Class of Gene
nuclear_gene
(Drosophila Heterochromatin Genome Project, 2007)
protein_coding_gene
(Drosophila Heterochromatin Genome Project, 2007)
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
kl-3-RC
FBtr0346771
NM_001111012
13882
4593
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kl-3 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (11 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
ATP binding
inferred from electronic annotation with InterPro:IPR011704
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
ATP-dependent microtubule motor activity, minus-end-directed
inferred from sequence or structural similarity with UniProtKB:A8JDH8
(FlyBase Curators, 2008--)
inferred from biological aspect of ancestor with PANTHER:PTN000743491
(assigned by GO_Central )
(Gaudet et al., 2011)
ATPase activity, coupled
inferred from sequence or structural similarity with FLYBASE:CG9492; FB:FBgn0037726
(de Carvalho et al., 2000)
traceable author statement
(de Carvalho et al., 2000)
dynein intermediate chain binding
inferred from biological aspect of ancestor with PANTHER:PTN000743491
(assigned by GO_Central )
(Gaudet et al., 2011)
dynein light intermediate chain binding
inferred from biological aspect of ancestor with PANTHER:PTN000743491
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
cilium movement
inferred from sequence or structural similarity with UniProtKB:A8JDH8
(FlyBase Curators, 2008--)
microtubule-based movement
inferred from biological aspect of ancestor with PANTHER:PTN000743491
(assigned by GO_Central )
(Gaudet et al., 2011)
outer dynein arm assembly
inferred from biological aspect of ancestor with PANTHER:PTN002472868
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
axonemal dynein complex
inferred from mutant phenotype
(Goldstein et al., 1982)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
dynein complex
inferred from biological aspect of ancestor with PANTHER:PTN000743491
(assigned by GO_Central )
(Gaudet et al., 2011)
outer dynein arm
inferred from sequence or structural similarity with UniProtKB:Q8TE73
(FlyBase Curators, 2008--)
inferred from biological aspect of ancestor with PANTHER:PTN002472868
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
axonemal dynein complex
inferred from mutant phenotype
(Goldstein et al., 1982)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\kl-3 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 24 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of kl-3
Transgenic constructs containing regulatory region of kl-3
Deletions and Duplications ( 73 )
Disrupted in
Df(YL)A10
(Hazelrigg et al., 1982)
Df(YL)A11
(Hazelrigg et al., 1982)
Df(YL)A17
(Hazelrigg et al., 1982)
Df(YL)A32
(Hazelrigg et al., 1982)
Df(YL)A39
(Hazelrigg et al., 1982)
Df(YL)A49
(Hazelrigg et al., 1982)
Df(YL)B4
(Hazelrigg et al., 1982)
Df(YL)B12
(Hazelrigg et al., 1982)
Df(YL)B14
(Hazelrigg et al., 1982)
Df(YL)G19
(Hardy et al., 1984)
Df(YL)G20
(Hardy et al., 1984)
Df(YL)G21
(Hardy et al., 1984)
Df(YL)G22
(Hardy et al., 1984)
Df(YL)G23
(Hardy et al., 1984)
Df(YL)G24
(Hardy et al., 1984)
Df(YL)H121
(Bonaccorsi and Lohe, 1991)
Df(YL)S7
(Gatti and Pimpinelli, 1983)
Df(YL)S8
(Gatti and Pimpinelli, 1983)
Df(YL)S9
(Gatti and Pimpinelli, 1983)
Df(YL)S10
(Gatti and Pimpinelli, 1983)
Df(YL)S11
(Gatti and Pimpinelli, 1983)
Df(YL)S13
(Gatti and Pimpinelli, 1983)
Df(YL)S14
(Gatti and Pimpinelli, 1983)
In(YL)B2
(Gatti and Pimpinelli, 1983)
In(YL)S15
(Gatti and Pimpinelli, 1983)
In(YL)S16L
(Gatti and Pimpinelli, 1983)
T(1;Y)R39
T(1;Y)S1
T(1;Y)S2
T(1;Y)S24
T(1;Y)T13
(Bonaccorsi and Lohe, 1991)
T(1;Y)V57
T(Y;2)G74
(Bonaccorsi and Lohe, 1991, Gatti and Pimpinelli, 1983)
T(Y;2)P57
(Bonaccorsi and Lohe, 1991, Gatti and Pimpinelli, 1983)
T(Y;3)D107
(Gatti and Pimpinelli, 1983)
T(Y;3)S20
(Gatti and Pimpinelli, 1983)
Ts(1Lt;YSt)E15
(Zhang and Stankiewicz, 1998)
Ts(1Lt;YSt)F12
(Zhang and Stankiewicz, 1998)
Ts(1Lt;YSt)T13
(Bonaccorsi et al., 1990)
Ts(1Lt;YSt)W27
(Zhang and Stankiewicz, 1998)
Ts(1Rt;YLt)V24
(Bonaccorsi et al., 1990)
Ts(1Rt;YSt)V8
(Bonaccorsi et al., 1990)
Ts(1Rt;YSt)W19
(Bonaccorsi et al., 1990)
Ts(YSt;4Rt)B15
(Hazelrigg et al., 1982)
Not disrupted in
Df(YL)S5
(Gatti and Pimpinelli, 1983)
Df(YL)S6
(Gatti and Pimpinelli, 1983)
Df(YS)A6
(Hazelrigg et al., 1982)
Df(YS)B13
(Hazelrigg et al., 1982)
In(YL)B3
(Gatti and Pimpinelli, 1983)
In(YLS)A33
(Hazelrigg et al., 1982)
In(YLS)B1
(Gatti and Pimpinelli, 1983)
In(YLS)S18
(Gatti and Pimpinelli, 1983)
T(1;Y)V24
(Gatti and Pimpinelli, 1983)
T(Y;2)A169
(Gatti and Pimpinelli, 1983)
T(Y;2)B3
(Hazelrigg et al., 1982)
T(Y;2)B13
(Hazelrigg et al., 1982)
T(Y;2)B92
(Gatti and Pimpinelli, 1983)
T(Y;2)B242
(Gatti and Pimpinelli, 1983)
T(Y;2)J146
(Gatti and Pimpinelli, 1983)
T(Y;2)R155
(Gatti and Pimpinelli, 1983)
T(Y;3)A23
(Gatti and Pimpinelli, 1983)
T(Y;3)A34
(Hazelrigg et al., 1982)
T(Y;3)A112
(Gatti and Pimpinelli, 1983)
T(Y;3)B115
(Gatti and Pimpinelli, 1983)
T(Y;3)B223
(Gatti and Pimpinelli, 1983)
T(Y;3)B229
(Gatti and Pimpinelli, 1983)
T(Y;3)S21
(Gatti and Pimpinelli, 1983)
Ts(1Lt;YLt)V8
(Zhang and Stankiewicz, 1998)
Ts(1Lt;YLt)W19
(Zhang and Stankiewicz, 1998)
Ts(1Lt;YSt)V24
(Zhang and Stankiewicz, 1998)
Ts(YLt;3Lt)W27
(Bonaccorsi et al., 1990)
Ts(YLt;3Rt)A34
(Hazelrigg et al., 1982)
Ts(YLt;4Lt)B15
(Hazelrigg et al., 1982)
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\DNAH5
3 of 15
Yes
No
Hsap\DNAH8
2 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Dnah5
2 of 15
Yes
No
Mmus\Dnah8
2 of 15
Yes
No
Rattus norvegicus (Norway rat) (2)
Rnor\Dnah5
1 of 13
Yes
No
Rnor\Dnah8
1 of 13
Yes
No
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (2)
Drer\dnah5
2 of 15
Yes
No
Drer\dnah5l
2 of 15
Yes
No
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\DYN1
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\dhc1
2 of 12
Yes
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( None identified )
No orthologies identified
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915001A )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17015443
Glossina morsitans
Tsetse fly
Gmor\GMOY004878
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_05250
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007225
Anopheles darlingi
American malaria mosquito
Adar\ADAC001899
Anopheles gambiae
Malaria mosquito
Agam\AGAP007675
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ012527
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W000Z )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA002144
Bombyx mori
Silkmoth
Bmor\BGIBMGA003690
Bombyx mori
Silkmoth
Bmor\BGIBMGA002148
Danaus plexippus
Monarch butterfly
Dple\DPOGS203998-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS203785-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS201355-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS203786-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL012849
Heliconius melpomene
Postman butterfly
Hmel\HMEL005947
Heliconius melpomene
Postman butterfly
Hmel\HMEL013863
Apis florea
Little honeybee
Aflo\2147496531
Apis florea
Little honeybee
Aflo\3731910980
Apis mellifera
Western honey bee
Amel\GB54468
Apis mellifera
Western honey bee
Amel\GB49507
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP15694
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP21035
Bombus terrestris
Buff-tailed bumblebee
Bter\15202990
Bombus terrestris
Buff-tailed bumblebee
Bter\15211333
Bombus terrestris
Buff-tailed bumblebee
Bter\15212790
Bombus terrestris
Buff-tailed bumblebee
Bter\15211332
Linepithema humile
Argentine ant
Lhum\LH21811
Linepithema humile
Argentine ant
Lhum\LH18089
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0029e0
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000f49
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002cd3
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG013890
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG017694
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_04556
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_09854
Tribolium castaneum
Red flour beetle
Tcas\TC000076
Tribolium castaneum
Red flour beetle
Tcas\TC034619
Tribolium castaneum
Red flour beetle
Tcas\TC000077
Pediculus humanus
Human body louse
Phum\PHUM247970
Pediculus humanus
Human body louse
Phum\PHUM561990
Rhodnius prolixus
Kissing bug
Rpro\RPRC004988
Cimex lectularius
Bed bug
Clec\CLEC008864
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005174
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_07035
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X000Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR007660
Strigamia maritima
European centipede
Smar\SMAR010821
Stegodyphus mimosarum
African social velvet spider
Smim\KFM58683.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM83564.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM57315.1
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_316462
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G001E )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Dnahc5
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Axndhc8h
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Axndhc15h
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_009127gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Axndhc5h
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Unk_101
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Dnah5
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_014620gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Dnah5-2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Dnah5_1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000008991
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000004223
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000024018
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000013268
Gallus gallus
Domestic chicken
Ggal\DNAH5
Gallus gallus
Domestic chicken
Ggal\DNAH8
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (12)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
btv
2 of 10
Dhc93AB
2 of 10
kl-2
2 of 10
CG3339
1 of 10
CG9492
1 of 10
CG14651
1 of 10
Dhc16F
1 of 10
Dhc36C
1 of 10
Dhc62B
1 of 10
Dhc64C
1 of 10
Dhc98D
1 of 10
Dnah3
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    DNAH5; dynein axonemal heavy chain 5
    model of  primary ciliary dyskinesia 3
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    DNAH5; dynein axonemal heavy chain 5
    3 of 15
    603335
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    Y
    Recombination map

    Y-

    Cytogenetic map
    h8-h8
    Sequence location
    Y:336,381..563,165 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    h8-h8
    Left limit from complementation mapping against T(Y;3)S20 (FBrf0039037) Right limit from complementation mapping against T(Y;3)D107 (FBrf0039037)
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    h7-h9
    (Drosophila Heterochromatin Genome Project, 2004, Zhang et al., 1996)
    Maps to the region of kl-3.
    (Zhang, 1996.4.28)
    h7-h9
    (determined by in situ hybridisation)
    (Zhang and Stankiewicz, 1998)
    Experimentally Determined Recombination Data
    Location

    Y-

    (Hackstein and Hochstenbach, 1995)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (5)
    Genomic Clones (3)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (17)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of

        Source for merge of: kl-3 CG17629

        (FlyBase, 1996-)

        Source for merge of: kl-3 CG40992 CG40936

        (FlyBase Genome Annotators, 2014)
        Additional comments

        Annotations CG40992, CG40936 and CG17629 merged as CG45785 in release 6.02 of the genome annotation. This gene is no longer fragmented in the release 6 genome assembly.

        (FlyBase Genome Annotators, 2014)

        Source for merge of kl-3 CG17629 was sequence comparison ( date:001108 ).

        (FlyBase, 1996-)

        Williamson (1972) reported 23 non-complementing and nine complementing alleles induced by EMS.

        Other Comments

        Sequence of transcript(s) changed in r6 genomic release relative to r5 release.

        (FlyBase Genome Annotators, 2014)

        New annotation (CG40992) in release 5.2 of the genome annotation.

        (Drosophila Heterochromatin Genome Project, 2007)

        New annotation (CG40936) in release 5.2 of the genome annotation.

        (Drosophila Heterochromatin Genome Project, 2007)

        Transiently named CG40443 in release 3 of the genome annotation.

        (FlyBase Genome Annotators, 2002-2003)

        It is not yet known if "ms(Y)104b" is the same as kl-3.

        (Zhang, 1996.4.28)

        Primary spermatocyte nuclei exhibit three giant lampbrush-like loops formed by the kl-5, kl-3 and ks-1 Y chromosome fertility factors.

        (Pisano et al., 1993)

        Males mutant for kl-3 fail to assemble the outer dynein arms associated with the peripheral nine microtubule doublets of the sperm-tail axoneme (Hardy, Tokuyasu, and Lindsley, 1981). Such males also fail to produce a kl-3-specific 300-325 kilodalton sperm polypeptide presumed to be a component of the outer dynein arms (Goldstein, Hardy and Lindsley, 198). Such mutants display an exceedingly low level of fertility at 25oC but not at 18oC immediately upon eclosion, but not thereafter (Kennison, 1983). Deficiencies for kl-3 are completely sterile, and in addition to the above mutant phenotype, they fail to elaborate the loops (ribbon-like structure) observed in the primary spermatocyte nuclei of normal males by light microscopy and the reticular material ordinarily observed by electron microscopy in spermatocyte nuclei (Hardy et al., 1981). The kl-3 loops are visible in living spermatocytes and in fixed cells stained with the protein-specific dye CBB; these loops are also demonstrated by the polyclonal antibody Sph-155 but do not react with monoclonal antibody S5 (Bonaccorsi et al., 1988).

        Origin and Etymology
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        External Crossreferences and Linkouts ( 33 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        Synonyms and Secondary IDs (17)
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