Dhc-Yh3, kl5, Lms4, DhcYh3
Gene model reviewed during 6.01
Gene model reviewed during 6.02
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kl-5 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\kl-5 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Annotations CG40444 and CG41497 merged as CG45786 in release 6.02 of the genome annotation. This gene is no longer fragmented in the release 6 genome assembly.
Annotations CG12573 and CG17616 merged as CG40444 in release 3 of the genome annotation.
Williamson (1972) reported 26 non-complementing and nine complementing alleles induced by EMS.
Sequence of transcript(s) changed in r6 genomic release relative to r5 release.
New annotation (CG41497) in release 5.2 of the genome annotation.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Identification: PCR screen for Dynein heavy chain genes.
kl-5 gene locus encodes a dynein heavy chain, the dynein heavy chain sequences are localised to the kl-5 region of the Y chromosome. These results provide evidence that at least one of the Y chromosome fertility regions encodes a polypeptide required for spermatogenesis.
AAGAC satellite DNA repeats which map to the kl-5 and ks-1 loop forming regions are abundantly transcribed in primary spermatocytes. These transcripts are detected at all stages of development of these two loops, do not appear to migrate to the cytoplasm and are degraded when the loops disintegrate during the first meiotic prophase.
Males mutant for kl-5 fail to assemble the outer dynein arms associated with the A tubules of the peripheral doublets of the sperm tail axoneme (Hardy, Tokuyasu, and Lindsley, 1981). Such males also fail to produce kl-5-specific 300-325 kilodalton sperm polypeptide considered to be a component of the outer arms (Goldstein, Hardy and Lindsley, 1982). kl-5 mutants exhibit very low level of fertility at 25oC but not at 18oC and in young but not older males (Kennison, 1983). Deficiencies for kl-5 completely sterile and in addition to the mutant phenotype they fail to produce aggregates of tubuli normally seen in thin sections of primary spermatocyte nuclei (Hardy et al., 1981). Within the kl-5 fertility region of the spermatocyte nucleus, a loop-forming site has been demonstrated by its reaction with the monoclonal antibody S5 (Bonaccorsi et al., 1988). These loops are stained with the protein-specific dye CBB, but are not visible in living preparations.