flam, lincRNA.1041, XLOC_024457, COM
Transcripts flam-RA through flam-RJ characterized by 5' RACE, but 3' ends have not been determined (FBrf0224576).
Gene model reviewed during 6.02
Gene is a putative piRNA precursor.
Evidence exists for additional alternatively spliced transcripts (FBrf0224576); additional unannotated transcripts may have noncanonical splices.
Gene model supported by FBrf0224576.
Gene model reviewed during 6.03
lncRNA ; SO:0001877
Corresponds to XLOC_024457 (FBrf0240702).
Gene model reviewed during 6.32
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lncRNA:flam using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\lncRNA:flam in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: lncRNA:flam flam
Source for merge of: flam CR46037
New annotation (CR46037) in release 6.02 of the genome annotation.
The proportion of normally copulating males is reduced in flam mutants compared to wild type. This reduction is due to changes in male behaviour. The proportion of males that perform consecutive courtship stages is significantly lower than in wild type. The sequence and duration of some courtship stages (in particular, orientation and wing vibration) is altered in mutant flies.
Cell lines carrying the permissive flam1 allele accumulate many nuclear virus-like particles, cytoplasmic dense particles and cisternae filled with fibrous material. Two of three such cell lines have an increased copy number of the gypsy element.
In some stocks active gypsy elements are found restricted to the Y chromosome. The presence of flam alleles tends to be associated with the confinement of active gypsy elements to the Y chromosome. This might be a result of the female-specific effect of flam on gypsy activity.
The length of time the existence of endogenous genomic gypsy was accompanied by host-virus co-evolution is studied. Host-virus co-evolution probably led to the acquisition by flam gene of the ability to control gypsy multiplication.
The expression of gypsy encoded proteins is analysed to explore how gypsy is transmitted between generations. Assembly of gypsy particles is visualised in the follicle cells of flam females by electron microscopy, these observations provide the basis for a novel model to explain how gypsy is transmitted from generation to generation. gypsy virions appear to move through the perivitelline space during a brief developmental window and infect the oocyte, providing a mechanism to explain gypsy insertion in the next generation.
gypsy can be transmitted from flam strains in which it transposes to strains devoid of functional elements by egg plasm transfer or growing "empty" larvae in the presence of homogenized pupae of the flam stock. Transposition of gypsy occurs only in the progeny of females homozygous for permissive alleles of flam, where gypsy transcripts are restricted to the somatic follicle cells in the ovaries. Infectious particles which then infect the oocyte are apparently produced in these cells.
Production of the gypsy\env message and corresponding protein is strongly repressed by one copy of the non-permissive allele of flam, flamN. A less dramatic reduction in the accumulation of other transcripts and retrotranscripts is also observed. These effects correlate with the inhibition of gypsy transposition in the progeny of these females and is likely to be responsible for this phenomenon. The effects of flam on gypsy\env expression are restricted to the somatic follicle cells that surround the maternal germline.