FB2020_01, released Feb 12, 2020

Gene: Dmel\CanA-14F

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General Information
Symbol
Dmel\CanA-14F
Species
D. melanogaster
Name
Calcineurin A at 14F
Annotation Symbol
CG9819
Feature Type
protein_coding_gene
FlyBase ID
FBgn0267912
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Snapshot
In progress.Contributions welcome.
Other Summaries
Also Known As

MRE12, Calcineurin

Key Links
Genomic Location
Cytogenetic map
14E3-14F1
Sequence location
X:16,560,684..16,587,172 [-]
Recombination map

1-54

RefSeq locus
NC_004354 REGION:16560684..16587172
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the PPP phosphatase family. PP-2B subfamily. (Q9VXF1)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
calmodulin-dependent protein phosphatase activity; protein binding
Predictions / Assertions
calmodulin binding; calmodulin-dependent protein phosphatase activity; protein serine/threonine phosphatase activity
Summaries
Gene Group (FlyBase)
PHOSPHOPROTEIN PHOSPHATASES -
The Phosphoprotein Phosphatase (PPP) family members are serine/threonine phosphatases. They often function in association with regulatory subunits. (Adapted from PMID:11230548 and PMID:19879837).
Protein Function (UniProtKB)
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
(UniProt, Q9VXF1)
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\CanA-14F or the JBrowse view of Dmel\CanA-14F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.46

Gene model reviewed during 5.41

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
retrotransposed_protein_coding_gene
(dos Santos, 2014, Miskei et al., 2011, Betran et al., 2002)
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
CanA-14F-RA
FBtr0074292
NM_167523
3052
584
CanA-14F-RB
FBtr0074293
NM_167524
6837
584
CanA-14F-RC
FBtr0305198
NM_001258788
7965
584
CanA-14F-RD
FBtr0332870
NM_001272693
4007
584
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
CanA-14F-PA
FBpp0074067
64.3
584
5.96
NP_727985
AAF48623
CanA-14F-PB
FBpp0074068
64.3
584
5.96
NP_727986
AAN09410
CanA-14F-PC
FBpp0293728
64.3
584
5.96
NP_001245717
AFH07430
CanA-14F-PD
FBpp0305092
64.3
584
5.96
NP_001259622
AGB95464
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

584 aa isoforms: CanA-14F-PA, CanA-14F-PB, CanA-14F-PC, CanA-14F-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with sra in a complex that contains Pp2B-14D.

(UniProt, Q9VXF1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CanA-14F using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (8 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
calmodulin-dependent protein phosphatase activity
inferred from mutant phenotype
(assigned by UniProt )
(Takeo et al., 2006)
protein binding
inferred from physical interaction with UniProtKB:Q9XZL8
(assigned by UniProt )
(Takeo et al., 2012, Takeo et al., 2006)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
calmodulin binding
inferred from biological aspect of ancestor with PANTHER:PTN000746105
(assigned by GO_Central )
(Gaudet et al., 2011)
calmodulin-dependent protein phosphatase activity
inferred from biological aspect of ancestor with PANTHER:PTN000746105
(assigned by GO_Central )
(Gaudet et al., 2011)
protein serine/threonine phosphatase activity
inferred from sequence or structural similarity
(Chen et al., 1992)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
sleep
inferred from direct assay
(Nakai et al., 2011, Tomita et al., 2011)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
calcineurin-mediated signaling
inferred from biological aspect of ancestor with PANTHER:PTN000746105
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
calcineurin complex
inferred from biological aspect of ancestor with PANTHER:PTN000746105
(assigned by GO_Central )
(Gaudet et al., 2011)
cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000746105
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult optic lobe
(Tomita et al., 2011)
adult brain
(Tomita et al., 2011)
retina
(Tomita et al., 2011)
Additional Descriptive Data

CanA-14F is widely expressed in the adult brain, optic lobe, and retina.

(Tomita et al., 2011)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CanA-14F in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CanA-14F
Transgenic constructs containing regulatory region of CanA-14F
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PPP3CA
14 of 15
Yes
No
 
Hsap\PPP3CB
12 of 15
No
Yes
Hsap\PPP3CC
12 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Ppp3ca
14 of 15
Yes
No
Mmus\Ppp3cb
11 of 15
No
Yes
Mmus\Ppp3cc
10 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
Rnor\Ppp3ca
11 of 13
Yes
Yes
Rnor\Ppp3cb
8 of 13
No
Yes
Rnor\Ppp3cc
8 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\ppp3ca
12 of 12
Yes
Yes
Xtro\ppp3cc
9 of 12
No
Yes
Xtro\ppp3cb
6 of 12
No
Yes
Danio rerio (Zebrafish) (4)
Drer\ppp3ca
11 of 15
Yes
Yes
Drer\ppp3cca
10 of 15
No
Yes
Drer\ppp3ccb
10 of 15
No
Yes
Drer\ppp3cb
5 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\tax-6
14 of 15
Yes
No
Cele\tba-2
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\CNA1
13 of 15
Yes
Yes
Scer\CMP2
12 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\ppb1
12 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919060Y )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\CanA-14F
Drosophila melanogaster
fruit fly
Dmel\Pp2B-14D
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00008222
Drosophila sechellia
Dsec\GM13389
Drosophila sechellia
Dsec\GM13390
Drosophila erecta
Dere\GG18247
Drosophila erecta
Dere\GG18246
Drosophila yakuba
Dyak\GE15960
Drosophila ananassae
Dana\GF20796
Drosophila ananassae
Dana\GF20794
Drosophila pseudoobscura pseudoobscura
Dpse\GA22069
Drosophila persimilis
Dper\GL19972
Drosophila willistoni
Dwil\GK25243
Drosophila willistoni
Dwil\GK25442
Drosophila virilis
Dvir\GJ19343
Drosophila mojavensis
Dmoj\GI15153
Drosophila mojavensis
Dmoj\GI15154
Drosophila grimshawi
Dgri\GH24890
Drosophila grimshawi
Dgri\GH24889
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503T8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17011595
Musca domestica
House fly
Mdoa\17011777
Musca domestica
House fly
Mdoa\17018175
Glossina morsitans
Tsetse fly
Gmor\GMOY008907
Glossina morsitans
Tsetse fly
Gmor\GMOY008078
Glossina morsitans
Tsetse fly
Gmor\GMOY007597
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10408
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11822
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_02720
Mayetiola destructor
Hessian fly
Mdes\Mdes002974
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL008930
Anopheles darlingi
American malaria mosquito
Adar\ADAC008703
Anopheles gambiae
Malaria mosquito
Agam\AGAP001279
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ018692
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03LH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\cna
Danaus plexippus
Monarch butterfly
Dple\DPOGS206069-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL005036
Apis florea
Little honeybee
Aflo\2201296148
Apis mellifera
Western honey bee
Amel\LOC411046
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP18082
Bombus terrestris
Buff-tailed bumblebee
Bter\15216049
Linepithema humile
Argentine ant
Lhum\LH25683
Linepithema humile
Argentine ant
Lhum\LH17508
Linepithema humile
Argentine ant
Lhum\LH17500
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0011e2
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG007022
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG007393
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_09979
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_09950
Tribolium castaneum
Red flour beetle
Tcas\TC000103
Pediculus humanus
Human body louse
Phum\PHUM596230
Rhodnius prolixus
Kissing bug
Rpro\RPRC009380
Cimex lectularius
Bed bug
Clec\CLEC001543
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI003343
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_08435
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03IC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ixodes scapularis
Black-legged tick
Isca\ISCW024405
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63371.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM80850.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur04g07540
Tetranychus urticae
Two-spotted spider mite
Turt\tetur04g07560
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_62512
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_312053
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G094R )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Cn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Cnbs
Ciona intestinalis
Vase tunicate
Cint\LOC100185597
Gallus gallus
Domestic chicken
Ggal\LOC769842
Gallus gallus
Domestic chicken
Ggal\PPP3CB
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (2)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Pp2B-14D
9 of 10
CanA1
7 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PPP3CA; protein phosphatase 3 catalytic subunit alpha
    14 of 15
    114105
     
    PPP3CB; protein phosphatase 3 catalytic subunit beta
    12 of 15
    114106
        PPP3CC; protein phosphatase 3 catalytic subunit gamma
        12 of 15
        114107
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            CanA-14F - Cam
            lambda phage display
            (Xu et al., 1998)
            CanA-14F - Pvr
            anti tag coimmunoprecipitation, peptide massfingerprinting
            (Friedman et al., 2011)
            CanA-14F - dos
            anti tag coimmunoprecipitation, peptide massfingerprinting
            (Friedman et al., 2011)
            CanA-14F - sra
            anti tag coimmunoprecipitation, western blot, multidimensional protein identification technology
            (Takeo et al., 2012)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            CanA-14F
            enhanceable
            sra
            (Nakai et al., 2011)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            sra
            suppressible
            CanA-14F
            (Nakai et al., 2011)
            External Data
            Subunit Structure (UniProtKB)
            Interacts with sra in a complex that contains Pp2B-14D.
            (UniProt, Q9VXF1 )
            Linkouts
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Linkouts
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            X
            Recombination map

            1-54

            Cytogenetic map
            14E3-14F1
            Sequence location
            X:16,560,684..16,587,172 [-]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            14E3-14F1
            Limits computationally determined from genome sequence between P{EP}EP1547EP1547 and P{EP}CG4928EP1341
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            14D-14D
            (Hong, 1995.6.28)
            14D-14F
            (determined by in situ hybridisation)
            (Madueno et al., 1995)
            Experimentally Determined Recombination Data
            Location

            1-54

            (FlyBase, 2016)

            1-55.5

            (Comeron et al., 2012)

            1-

            (Hong, 1995.6.28)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (8)
            Genomic Clones (14)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (21)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequences
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Laboratory Generated Antibodies
               
              Commercially Available Antibodies
               
              Other Information
              Relationship to Other Genes
              Source for database identify of

              Source for identity of: CanA-14F CG9819

              (Sullivan and Rubin, 2002)
              Source for database merge of

              Source for merge of: CanA-14F MRE12

              (FlyBase Genome Annotators, 2014)
              Additional comments

              MRE12 corresponds to a portion of the 3' UTR of CanA-14F.

              (FlyBase Genome Annotators, 2014)

              The CanA-14F gene may have been derived from a related phosphoprotein phosphatase gene by retroposition and subsequent tandem duplication.

              (Miskei et al., 2011)

              The CanA-14F gene may be derived from the CanA1 gene by retroposition.

              (Betran et al., 2002)
              Other Comments
              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 61 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              Linkouts
              KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Synonyms and Secondary IDs (18)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (68)