FlyBase Gene Report: Dmel\Dsp1

POLYCOMB GROUP RECRUITERS/DNA-BINDING PROTEINS -: The Polycomb group (PcG) proteins are epigenetic regulators, best characterized for the repression of Hox gene expression. In Drosophila, PcG proteins repress their target genes by binding to specific DNA elements called Polycomb Response Elements (PREs). PcG DNA binding proteins contribute to the recruitment of Polycomb complexes to PREs. (Adapted from FBrf0228921).
HIGH MOBILITY GROUP BOX TRANSCRIPTION FACTORS -: The High mobility group box (HMGB) transcription factors are sequence-specific DNA binding proteins that regulate transcription. The HMGB proteins have a characteristic L-shaped HMGB domain of about 80 amino acid residues, which binds the DNA minor groove and induce DNA bending. The HMGB domains are found in one or more copies and are involved in the regulation of DNA-dependent processes such as transcription, replication and chromatin remodeling. (Adapted from FBrf0194706, FBrf0108466, PMID:24086078 and PMID:23153957).

Protein Function (UniProtKB)

Binds preferentially single-stranded DNA and unwinds double-stranded DNA.

(UniProt, Q24537)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Dsp1 or the JBrowse view of Dmel\Dsp1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Dsp1-RA

FBtr0089260

NM_167503

3990

385

Dsp1-RB

FBtr0089259

NM_167504

3915

385

Dsp1-RC

FBtr0089261

NM_080715

4511

385

Dsp1-RD

FBtr0089262

NM_167502

2903

386

Dsp1-RE

FBtr0089263

NM_206762

4240

393

Dsp1-RF

FBtr0289960

NM_001144731

2205

397

Dsp1-RG

FBtr0339766

NM_001298393

2359

386

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Dsp1-PA

FBpp0088319

43.9

385

8.71

NP_727960

AAN09395

Dsp1-PB

FBpp0088318

43.9

385

8.71

NP_727961

AAN09396

Dsp1-PC

FBpp0088320

43.9

385

8.71

NP_542446

AAF48594

Dsp1-PD

FBpp0089257

44.0

386

8.71

NP_727959

AAN09394

Dsp1-PE

FBpp0089258

44.9

393

8.70

NP_996485

AAS65386

Dsp1-PF

FBpp0288398

45.3

397

8.70

NP_001138203

ACL82935

Dsp1-PG

FBpp0308813

44.0

386

8.71

NP_001285322

AHN59792

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

385 aa isoforms: Dsp1-PA, Dsp1-PB, Dsp1-PC

386 aa isoforms: Dsp1-PD, Dsp1-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dsp1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dsp1_1

Linkouts

Gene Ontology (25 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

DNA binding

inferred from direct assay

(Lehming et al., 1994)

DNA binding, bending

inferred from direct assay

(Janke et al., 2003)

protein binding

inferred from physical interaction with UniProtKB:P23497-3

(assigned by BHF-UCL )

(Lehming et al., 1998)

TBP-class protein binding

inferred from physical interaction with FLYBASE:Tbp; FB:FBgn0003687

(Kirov et al., 1996)

transcription factor binding

inferred from physical interaction with FLYBASE:dl; FB:FBgn0260632

(Decoville et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

DNA binding, bending

inferred from biological aspect of ancestor with PANTHER:PTN000345179

(assigned by GO_Central )

(Gaudet et al., 2011)

single-stranded DNA binding

non-traceable author statement

(assigned by UniProt )

(Lehming et al., 1994)

transcription factor binding

inferred from biological aspect of ancestor with PANTHER:PTN000345179

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (15 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

chromatin remodeling

inferred from mutant phenotype

(Decoville et al., 2001)

developmental process

inferred from mutant phenotype

(Mosrin-Huaman et al., 1998)

leg disc development

inferred from mutant phenotype

(Ragab et al., 2006)

negative regulation of antimicrobial humoral response

inferred from mutant phenotype

(Kim et al., 2007)

inferred from genetic interaction with FLYBASE:Rel; FB:FBgn0014018

(Kim et al., 2007)

negative regulation of RNA polymerase II transcriptional preinitiation complex assembly

inferred from direct assay

(Kirov et al., 1996)

negative regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Decoville et al., 2000)

inferred from direct assay

(Lehming et al., 1998, Lehming et al., 1994)

negative regulation of transcription, DNA-templated

inferred from direct assay

(assigned by BHF-UCL )

(Lehming et al., 1998)

positive regulation of DNA binding

inferred from direct assay

(Decoville et al., 2000, Lehming et al., 1994)

segment specification

inferred from mutant phenotype

(Decoville et al., 2001)

Terms Based on Predictions or Assertions (7 terms)

CV Term

Evidence

References

chromatin remodeling

inferred from biological aspect of ancestor with PANTHER:PTN000345179

(assigned by GO_Central )

(Gaudet et al., 2011)

DNA recombination

inferred from biological aspect of ancestor with PANTHER:PTN001009623

(assigned by GO_Central )

(Gaudet et al., 2011)

DNA unwinding involved in DNA replication

non-traceable author statement

(assigned by UniProt )

(Lehming et al., 1994)

positive regulation of innate immune response

inferred from biological aspect of ancestor with PANTHER:PTN001009623

(assigned by GO_Central )

(Gaudet et al., 2011)

positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN001009623

(assigned by GO_Central )

(Gaudet et al., 2011)

regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000346026

(assigned by GO_Central )

(Gaudet et al., 2011)

regulation of transcription, DNA-templated

inferred from biological aspect of ancestor with PANTHER:PTN000345179

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

nucleus

inferred from direct assay

(Mosrin-Huaman et al., 1998)

inferred from high throughput direct assay

(Lye et al., 2014)

polytene chromosome

inferred from direct assay

(Lamiable et al., 2010)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001009623

(assigned by GO_Central )

(Gaudet et al., 2011)

nuclear chromatin

inferred from biological aspect of ancestor with PANTHER:PTN000346026

(assigned by GO_Central )

(Gaudet et al., 2011)

nucleus

non-traceable author statement

(assigned by UniProt )

(Lehming et al., 1994)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

embryonic stage 1 -- 2

pole plasm

(Yatsu et al., 2008)

embryonic stage 4 -- 5

pole cell

(Yatsu et al., 2008)

embryonic stage 14 -- 16

pole cell

(Yatsu et al., 2008)

embryonic stage 5

organism | ubiquitous

(Mosrin-Huaman et al., 1998)

embryonic stage 9

organism | ubiquitous

(Mosrin-Huaman et al., 1998)

embryonic stage 13

embryonic/larval central nervous system

(Mosrin-Huaman et al., 1998)

embryonic/larval anterior midgut

(Mosrin-Huaman et al., 1998)

embryonic/larval posterior midgut

(Mosrin-Huaman et al., 1998)

embryonic stage 16

ventral nerve cord

(Mosrin-Huaman et al., 1998)

embryonic/larval brain

(Mosrin-Huaman et al., 1998)

Additional Descriptive Data

Dsp1 transcripts are first detected at the cellular blastoderm stage in every cell. During germband retraction, staining is localized to the epidermis and the CNS. At embryonic stage 15-16, Dsp1 transcripts are restricted to the ventral nerve cord and brain.

(Mosrin-Huaman et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

ventral nerve cord

(Mosrin-Huaman et al., 1998)

oogenesis stage S10

nurse cell

• cytoplasm

(Mosrin-Huaman et al., 1998)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Mosrin-Huaman et al., 1998)

adult stage

adult head

(Mosrin-Huaman et al., 1998)

larval stage

embryonic/larval brain

(Mosrin-Huaman et al., 1998)

embryonic stage

organism | ubiquitous

(Mosrin-Huaman et al., 1998)

Additional Descriptive Data

Dsp1 protein is detected by western blot in ovary and adult head but not in testis, at all embryonic stages and in larval brain but not in Malpighian tubules, midgut, or salivary glands. Dsp1 protein is first detected by immunolocalization in egg chambers at oogenesis stage 10 where it is found in nurse cells in the cytoplasm. In third instar larvae it is observed at the extremity of the ventral ganglion but not in other parts of the brain.

(Mosrin-Huaman et al., 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

nucleus

inferred from direct assay

(Mosrin-Huaman et al., 1998)

inferred from high throughput direct assay

(Lye et al., 2014)

polytene chromosome

inferred from direct assay

(Lamiable et al., 2010)

Expression Deduced from Reporters

Reporter: PBac{CPTI-un}Dsp1^CPTI002844

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

Comment: cell bodies in adult brain cortex and glial sheath

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Dsp1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Dsp1

View exonic expression by tissue for Dmel\Dsp1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011764

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12223

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Dsp1

Transgenic constructs containing regulatory region of Dsp1

Deletions and Duplications ( 2 )

Disrupted in

Df(1)19

(Decoville et al., 2001)

Duplicated in

Dp(1;Y)shi⁺3

(Decoville et al., 2001)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^29BD

Dsp1^GS1115

lethal, with Scer\GAL4^Bx-MS1096

Dsp1^{UAS.ORF.GW.Tag:HA}

lethal, with Scer\GAL4^c355

Dsp1^GS1115

lethal, with Scer\GAL4^dpp.blk1

Dsp1^GS1115

lethal, with Scer\GAL4^hs.2sev

Dsp1^GS1115

lethal, with Scer\GAL4^pnr-MD237

Dsp1^GD4269

lethal | cold sensitive, with Scer\GAL4^Bx-MS1096

Dsp1^{UAS.ORF.GW.Tag:HA}

partially lethal

Dsp1¹

partially lethal | recessive

Dsp1¹

Sterility

Allele

semi-sterile

Dsp1¹

semi-sterile | recessive

Dsp1¹

Other Phenotypes

Allele

flight defective | recessive

Dsp1¹

locomotor behavior defective | recessive

Dsp1¹

neuroanatomy defective | larval stage, with Scer\GAL4^elav-C155

Dsp1^EP355

visible, with Scer\GAL4^ap-md544

Dsp1^EP355

visible, with Scer\GAL4^GMR.PF

Dsp1^EP355

visible | adult stage | homeotic | recessive

Dsp1¹

visible | homeotic

Dsp1¹

wild-type

Dsp1²⁷⁴

Phenotype manifest in

Allele

abdominal 4 ventral denticle belt

Dsp1¹

abdominal 5 ventral denticle belt

Dsp1¹

abdominal sternite bristle | ectopic

Dsp1¹

adult abdominal segment 4

Dsp1¹

adult abdominal segment 5 | ectopic

Dsp1¹

adult abdominal segment 6

Dsp1¹

adult metathoracic segment anterior compartment

Dsp1¹

adult thorax | dorsal, with Scer\GAL4^ap-md544

Dsp1^EP355

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Dsp1^EP355

egg

Dsp1¹

embryonic/first instar larval cuticle

Dsp1¹

eye, with Scer\GAL4^GMR.PF

Dsp1^EP355

haltere

Dsp1¹

intersegmental nerve, with Scer\GAL4^elav-C155

Dsp1^EP355

metathoracic laterotergite

Dsp1¹

metathoracic leg | ectopic

Dsp1¹

prothoracic leg

Dsp1¹

scutellum, with Scer\GAL4^ap-md544

Dsp1^EP355

scutum | ectopic

Dsp1¹

sex comb

Dsp1¹

wing, with Scer\GAL4^ap-md544

Dsp1^EP355

wing | ectopic

Dsp1¹

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\HMGB3

11 of 15

Yes

Hsap\HMGB2

10 of 15

Yes

Hsap\HMGB1

8 of 15

Yes

Hsap\HMGB4

4 of 15

Yes

Hsap\HMG20A

2 of 15

Hsap\HMG20B

2 of 15

Hsap\HMGB3P27

2 of 15

Yes

Hsap\HMGB1P1

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\UBTFL1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (15)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Hmgb3

11 of 15

Yes

Mmus\Hmgb2

10 of 15

Yes

Mmus\Gm21596

6 of 15

Yes

Mmus\Hmgb4

5 of 15

Yes

Mmus\Hmgb1

4 of 15

Yes

Mmus\Hmg20a

2 of 15

Mmus\Hmg20b

2 of 15

Mmus\Hmgb1l

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Ubtf

1 of 15

Yes

Rattus norvegicus (Norway rat) (46)

Rnor\Hmgb2l1

7 of 13

Yes

Rnor\RGD1559962

7 of 13

Yes

Rnor\Hmgb1

6 of 13

Yes

Rnor\Hmgb3

6 of 13

Yes

Rnor\LOC108350839

6 of 13

Yes

Rnor\Hmgb4

5 of 13

Yes

Rnor\LOC100912369

5 of 13

Yes

Rnor\Hmg1l1

4 of 13

Yes

Rnor\Hmgb2

4 of 13

Yes

Rnor\LOC108349258

4 of 13

Yes

Rnor\LOC681067

2 of 13

Yes

Rnor\LOC691030

2 of 13

Yes

Rnor\LOC100364877

2 of 13

Yes

Rnor\LOC103694516

2 of 13

Yes

Rnor\RGD1560585

2 of 13

Yes

Rnor\RGD1561558

2 of 13

Yes

Rnor\RGD1566225

2 of 13

Yes

Rnor\Hmg20a

1 of 13

Rnor\Hmg20b

1 of 13

Rnor\Hmgb1-ps3

1 of 13

Yes

Rnor\LOC688583

1 of 13

Yes

Rnor\LOC688702

1 of 13

Rnor\LOC100909696

1 of 13

Yes

Rnor\LOC100911637

1 of 13

Yes

Rnor\LOC102552204

1 of 13

Rnor\LOC103690880

1 of 13

Yes

Rnor\LOC103690901

1 of 13

Yes

Rnor\LOC103690902

1 of 13

Yes

Rnor\LOC103690904

1 of 13

Rnor\LOC103690905

1 of 13

Yes

Rnor\LOC103690909

1 of 13

Yes

Rnor\LOC103694517

1 of 13

Yes

Rnor\LOC103694587

1 of 13

Yes

Rnor\LOC108351135

1 of 13

Yes

Rnor\LOC108351411

1 of 13

Rnor\LOC108352140

1 of 13

Yes

Rnor\RGD1561694

1 of 13

Yes

Rnor\RGD1563668

1 of 13

Yes

Rnor\RGD1565430

1 of 13

Yes

Rnor\RGD1566008

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (11)

Xtro\hmgb3

10 of 12

Yes

Xtro\hmgb1

8 of 12

Yes

Xtro\hmgb2

4 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (16)

Drer\hmgb2b

10 of 15

Yes

Drer\hmgb1a

9 of 15

Yes

Drer\hmgb3a

9 of 15

Yes

Drer\hmgb1b

7 of 15

Yes

Drer\hmgb2a

7 of 15

Yes

Drer\hmgb3b

7 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\ubtfl

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\hmg-1.2

8 of 15

Yes

Cele\hmg-1.1

4 of 15

Cele\hmg-20

2 of 15

Cele\B0238.11

1 of 15

Yes

Cele\hmg-5

1 of 15

Cele\hmg-6

1 of 15

Arabidopsis thaliana (thale-cress) (7)

Atha\HMGB1

4 of 9

Yes

Atha\HMGB2

3 of 9

Atha\HMGB3

3 of 9

Atha\3xHMG-box1

2 of 9

Yes

Atha\3xHMG-box2

2 of 9

Yes

Atha\HMGB4

1 of 9

Atha\HMGB6

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\NHP6A

6 of 15

Yes

Scer\NHP6B

6 of 15

Yes

Scer\ABF2

4 of 15

Scer\IXR1

3 of 15

Scer\NHP10

3 of 15

Yes

Scer\HMO1

2 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\nhp6

6 of 12

Yes

Spom\SPBC28F2.11

4 of 12

Yes

Spom\cmb1

1 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190E86 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11488

Drosophila persimilis

Dper\GL15829

Drosophila willistoni

Dwil\GK25058

Drosophila virilis

Dvir\GJ19445

Drosophila mojavensis

Dmoj\GI10980

Drosophila grimshawi

Dgri\GH12282

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150ALF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17004734

Musca domestica

House fly

Mdoa\17013816

Glossina morsitans

Tsetse fly

Gmor\GMOY003446

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10947

Mayetiola destructor

Hessian fly

Mdes\Mdes008481

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011380

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL017077

Anopheles darlingi

American malaria mosquito

Adar\ADAC001494

Anopheles gambiae

Malaria mosquito

Agam\AGAP000005

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ014043

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CRH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001132

Danaus plexippus

Monarch butterfly

Dple\DPOGS205620-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007340

Apis florea

Little honeybee

Aflo\2634108548

Apis mellifera

Western honey bee

Amel\GB47828

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13111

Bombus terrestris

Buff-tailed bumblebee

Bter\15207715

Linepithema humile

Argentine ant

Lhum\LH12822

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00192a

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012176

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07692

Pediculus humanus

Human body louse

Phum\PHUM006290

Rhodnius prolixus

Kissing bug

Rpro\RPRC006379

Cimex lectularius

Bed bug

Clec\CLEC007928

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007949

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02651

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0CPH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR013695

Ixodes scapularis

Black-legged tick

Isca\ISCW017350

Stegodyphus mimosarum

African social velvet spider

Smim\KFM75735.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM80844.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g08260

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g08340

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_232871

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_306496

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0P81 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_027981gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Hmg2

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000004075

Gallus gallus

Domestic chicken

Ggal\HMG1

Gallus gallus

Domestic chicken

Ggal\HMGB2

Gallus gallus

Domestic chicken

Ggal\HMGB3

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (12)

Gene Symbol

Score

Source

Alignment

5 of 10

4 of 10

3 of 10

3 of 10

3 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Dsp1^EP355

exacerbates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.GMR.Tag:SS(rPENK)}

(Cao et al., 2008)

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HMGB3; high mobility group box 3

11 of 15

300193

MICROPHTHALMIA, SYNDROMIC 13; MCOPS13

HMGB2; high mobility group box 2

10 of 15

163906

HMGB1; high mobility group box 1

8 of 15

163905

HMGB4; high mobility group box 4

4 of 15

617285

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Dsp1 - CG4360

two hybrid

(Hu et al., 2017.6.13)

Dsp1 - Dll

two hybrid

(Hu et al., 2017.6.13)

Dsp1 - Dref

two hybrid array

(Shokri et al., 2019)

Dsp1 - HDAC1

anti bait coimmunoprecipitation, western blot

(Kim et al., 2007)

Dsp1 - Jra

anti bait coimmunoprecipitation, western blot

(Kim et al., 2007)

Dsp1 - Lim1

two hybrid

(Hu et al., 2017.6.13)

Dsp1 - Rm62

anti bait coimmunoprecipitation, Identification by mass spectrometry, cosedimentation through density gradient, western blot, anti tag coimmunoprecipitation

(Lamiable et al., 2010)

Dsp1 - Stat92E

anti bait coimmunoprecipitation, western blot

(Kim et al., 2007)

Dsp1 - corto

anti bait coimmunoprecipitation, western blot, far western blotting, autoradiography, pull down

(Salvaing et al., 2006)

Dsp1 - dl

pull down, autoradiography, electrophoretic mobility shift assay, far western blotting, one hybrid

(Decoville et al., 2000, Lehming et al., 1994)

Dsp1 - ens

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

RNA-protein

Physical Interaction

Assay

References

Dsp1 - Ythdc1

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Knuckles et al., 2018, Lence et al., 2016)

Dsp1 - fl(2)d

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Knuckles et al., 2018)

Dsp1 - nito

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Knuckles et al., 2018)

Dsp1 - stau

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Dsp1

enhanceable

ash1

(Decoville et al., 2001)

Dsp1

suppressible

(Decoville et al., 2001)

Dsp1

enhanceable

Scr

(Decoville et al., 2001)

Dsp1

enhanceable

trx

(Decoville et al., 2001)

Dsp1

enhanceable

Ubx

(Decoville et al., 2001)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Antp

suppressible

Dsp1

(Decoville et al., 2001)

btl|stumps

suppressible

Dsp1

(Zhu et al., 2005)

suppressible

Dsp1

(Müller et al., 2005)

enhanceable

Dsp1

(Decoville et al., 2001)

suppressible

Dsp1

(Decoville et al., 2001)

pnr

enhanceable

Dsp1

(Pena-Rangel et al., 2002)

enhanceable

(Zhu et al., 2005)

enhanceable

(Decoville et al., 2001)

Scr

suppressible

Dsp1

(Decoville et al., 2001)

vtd

suppressible

Dsp1

(Liu et al., 2015)

External Data

Linkouts

MIST (genetic) - An integrated Molecular Interaction Database

Dsp1

MIST (protein-protein) - An integrated Molecular Interaction Database

Dsp1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-53

Cytogenetic map

14C1-14B15

Sequence location

X:16,333,612..16,340,624 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

14C1-14B15

Left limit from molecular mapping relative to nonA (FBrf0051450) Right limit from molecular mapping relative to Cyp1 (FBrf0083559); Limits computationally determined from genome sequence between P{EP}Cyp1^EP1073&P{EP}Dsp1^EP355 and P{EP}EP1493^EP1493

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

14D-14D

(Locker, 1995.7.19, Locker, 1994.9.12)

14D1-14D4

(Canaple et al., 1997)

14C-14D

(determined by in situ hybridisation)

(Lehming et al., 1994)

Experimentally Determined Recombination Data

Location

1-53

(FlyBase, 2016)

1-54.5

(Comeron et al., 2012)

(Locker, 1995.7.19, Rutherford, 1995)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Bloomington

17270

w¹¹¹⁸ P{EP}Dsp1^EP355

85101

w¹¹¹⁸ P{XP}Dsp1^d07366

31960

y¹ v¹; P{TRiP.JF02732}attP2

83404

y¹ w^*; P{Dsp1-GFP.FPTB}attP40

FlyORF

F004554

M{UAS-Dsp1.ORF-CC}ZH-21F

F000072

M{UAS-Dsp1.ORF.3xHA.GW}ZH-86Fb

Kyoto

113403

w^* P{GawB}Dsp1^NP4141 / FM7c

115378

w¹¹¹⁸ PBac{CPTI-un}Dsp1^CPTI002844

VDRC

v101327

P{KK107579}VIE-260B

v41029

w¹¹¹⁸; P{GD4269}v41029

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (223)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

DPiM_GM02110

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0278608

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Dsp1 Hmg14D

(FlyBase, 1996-)

Source for merge of: Dsp1 Hmg14B

(FlyBase, 1992-)

Additional comments

"bss" stated to correspond to "Dsp1".

(Reynolds and Tanouye, 1998)

Source for merge of Dsp1 Hmg14D was sequence comparison ( date:000202 ).

(FlyBase, 1996-)

'Dsp1' was merged with 'Hmg14B' (which was associated with FBrf0083559 only) as 'Dsp1' is the only HMG box-encoding gene located in the 14B region.

(FlyBase, 1992-)

Other Comments

DNA-protein interactions: genome-wide binding profile assayed for Dsp1 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).

(Filion et al., 2010)

Dsp1 may act as a chromatin remodeling factor.

(Decoville et al., 2001)

Loss of Dsp1 function causes homeotic transformations resembling those associated with loss of function mutations in Scr, Ubx and Abd-B.

(Decoville et al., 2001)

Dsp1 protein binds cooperatively to Rel sites on DNA with NF-κΒ, the p50 subunit of the NF-κΒ heterodimer and with the Rel domain of dl. This cooperativity is unaffected by the presence of a negative regulatory element. Dif does not show high cooperative DNA binding with Dsp1 protein. dl can activate transcription from zen and twi promoters, and additional Dsp1 inhibits the zen activation and increases the twi activation.

(Brickman et al., 1999)

Dsp1 protein can act as a potent transcriptional repressor for multiple activator families in vitro and in transfection studies. Dsp1 represses activated transcription by interfering with the binding of TFIIA, a general transcription factor implicated in activated transcription pathways.

(Kirov et al., 1996)

Dsp1 can convert dl from a transcriptional activator to repressor. This effect requires the sequence termed a negative regulatory element (NRE) found adjacent to the dl binding sites in the zen promoter.

(Lehming et al., 1994)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 56 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

117294

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24537

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011764

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0278608

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dsp1_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12223

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG12223

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

Dsp1

MIST (protein-protein) - An integrated Molecular Interaction Database

Dsp1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG12223

(Keleman et al., 2009.8.5, Perkins et al., 2009, Grieder et al., 2007, Quinones-Coello, 2007, Kraut et al., 2001, Kraut et al., 2001, Whitfield, 2000.5.8)

HMGB1

(Liu et al., 2015)

Hmg14B

Hmg14D

Ssrp2

dsp1

(Schuettengruber et al., 2014, LOCKER and DECOVILLE, 2006, Muller and Kassis, 2006, Salvaing et al., 2006, Dejardin et al., 2005, Daulny et al., 2003, Decoville et al., 2001, Decoville et al., 2001)

hmg

(Rutherford, 1995)

ssrp2

(Locker, 1995.7.19)

Name Synonyms

Dorsal Switch Protein 1

(Schuettengruber et al., 2014, Lamiable et al., 2010, Brown et al., 2005)

Dorsal switch protein 1

(McElroy et al., 2014, Laver et al., 2013, Mason-Suares et al., 2013, Garcia and Stathopoulos, 2011, Lehming et al., 1994)

Dorsal switch protein-1

(Huen and Russell, 2010)

Hmg14B

Structure specific recognition protein 2

Secondary FlyBase IDs

FBgn0011764
FBgn0015237
FBgn0015301
FBgn0019980

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

transgene_used

FBGSE22069

genome binding

Protein profiling reveals five principal chromatin types in Drosophila cells.

References (128)

Report Sections

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