Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dsp1 using the Feature Mapper tool.
Dsp1 protein is detected by western blot in ovary and adult head but not in testis, at all embryonic stages and in larval brain but not in Malpighian tubules, midgut, or salivary glands. Dsp1 protein is first detected by immunolocalization in egg chambers at oogenesis stage 10 where it is found in nurse cells in the cytoplasm. In third instar larvae it is observed at the extremity of the ventral ganglion but not in other parts of the brain.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Dsp1 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
"bss" stated to correspond to "Dsp1".
Source for merge of Dsp1 Hmg14D was sequence comparison ( date:000202 ).
DNA-protein interactions: genome-wide binding profile assayed for Dsp1 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
Dsp1 protein binds cooperatively to Rel sites on DNA with NF-κΒ, the p50 subunit of the NF-κΒ heterodimer and with the Rel domain of dl. This cooperativity is unaffected by the presence of a negative regulatory element. Dif does not show high cooperative DNA binding with Dsp1 protein. dl can activate transcription from zen and twi promoters, and additional Dsp1 inhibits the zen activation and increases the twi activation.
Dsp1 protein can act as a potent transcriptional repressor for multiple activator families in vitro and in transfection studies. Dsp1 represses activated transcription by interfering with the binding of TFIIA, a general transcription factor implicated in activated transcription pathways.