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General Information
Symbol
Dmel\Pol32
Species
D. melanogaster
Name
Pol32 polymerase delta subunit
Annotation Symbol
CG3975
Feature Type
FlyBase ID
FBgn0283467
Gene Model Status
Stock Availability
Gene Snapshot
Pol32 polymerase delta subunit (Pol32) encodes a non-essential subunit of the multi-subunit DNA polymerases delta and zeta. It has roles during embryonic DNA replication and promotes several types of DNA repair, including homologous recombination repair of double-strand breaks. [Date last reviewed: 2019-03-14]
Also Known As
BG:DS07295.3 , rd, reduced
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:15,254,819..15,256,304 [-]
Recombination map
2-51
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Molecular Function (GO)
    [Detailed GO annotations]
    Experimental Evidence
    -
    Predictions / Assertions
    -
    Summaries
    Gene Group (FlyBase)
    DNA POLYMERASE DELTA -
    DNA polymerase delta functions as a leading strand polymerase during nuclear DNA synthesis. It is a multi-subunit enzyme in which DNApol-δ encodes the catalytic subunit with polymerase and 3'-5' exonuclease proofreading activities. (Adapted from PMID:30005324.)
    DNA POLYMERASE ZETA -
    DNA polymerase zeta is one of several translesion synthesis polymerases that act to promote replication through DNA lesions that would otherwise stall the replicative polymerases. It is a heterotetramer, in which the catalytic subunit is encoded by DNApol-ζ, and is specialized for the extension step of lesion bypass. (Adapted from PMID:30005324.)
    Phenotypic Description (Red Book; Lindsley and Zimm 1992)
    rd: reduced
    thumb
    rd: reduced
    From Bridges and Brehme, 1944, Carnegie Inst. Washington Publ. No. 552: 157.
    thumb
    rds: reduced-scraggly
    Edith M. Wallace, unpublished.
    Bristles reduced in length and thickness, sometimes twisted; best seen on sternopleurals; male more extreme than female (rd1); female may have abnormal abdominal banding (rds). Males fertile, but females may be sterile (rd1) (Lynch, 1919, Genetics 4: 501-33).
    Gene Model and Products
    Number of Transcripts
    2
    Number of Unique Polypeptides
    1

    Please see the GBrowse view of Dmel\Pol32 or the JBrowse view of Dmel\Pol32 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model
    Gene model reviewed during 5.42
    Gene model reviewed during 5.48
    Sequence Ontology: Class of Gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    FBtr0303164
    1486
    431
    FBtr0336864
    1391
    431
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    FBpp0292263
    47.0
    431
    9.98
    FBpp0307818
    47.0
    431
    9.98
    Polypeptides with Identical Sequences

    The group(s) of polypeptides indicated below share identical sequence to each other.

    431 aa isoforms: Pol32-PB, Pol32-PC
    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pol32 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Gene Ontology (7 terms)
    Molecular Function (0 terms)
    Terms Based on Experimental Evidence (0 terms)
    Terms Based on Predictions or Assertions (0 terms)
    Biological Process (4 terms)
    Terms Based on Experimental Evidence (4 terms)
    CV Term
    Evidence
    References
    Terms Based on Predictions or Assertions (0 terms)
    Cellular Component (3 terms)
    Terms Based on Experimental Evidence (2 terms)
    CV Term
    Evidence
    References
    inferred from direct assay
    inferred from direct assay
    Terms Based on Predictions or Assertions (1 term)
    CV Term
    Evidence
    References
    inferred from sequence or structural similarity with UniProtKB:Q15054
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    inferred from direct assay
    inferred from direct assay
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Pol32 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Images
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 16 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 12 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Pol32
    Transgenic constructs containing regulatory region of Pol32
    Deletions and Duplications ( 157 )
    Not disrupted in
    Disrupted in
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    1 of 15
    Yes
    Yes
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    1 of 15
    Yes
    Yes
    Rattus norvegicus (Norway rat) (1)
    1 of 13
    Yes
    Yes
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (1)
    1 of 15
    Yes
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (1)
    1 of 15
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (1)
    1 of 9
    Yes
    Yes
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (1)
    1 of 12
    Yes
    Yes
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190GM6 )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150C6J )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Paralogs (via DIOPT v7.1)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map
      2-51
      Cytogenetic map
      Sequence location
      2L:15,254,819..15,256,304 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      35C5-35C5
      Limits computationally determined from genome sequence between P{EP}vigEP812&P{PZ}stc05441 and P{EP}esgEP633&P{PZ}esg07082
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location
      2-51.3 +/- 0.2
      2-[51]
      Left of (cM)
      Notes
      Recombinants of gft06430 indicate that the gene order is rd, l(2)35Cc, gft.
      Stocks and Reagents
      Stocks (25)
      Genomic Clones (17)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (5)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        polyclonal
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for identity of: BG:DS07295.3 CG3975
        Source for identity of: Pol32 CG3975
        Source for database merge of
        Source for merge of: Pol32 rd
        Additional comments
        Other Comments
        Origin and Etymology
        Discoverer
        Bridges, 15th July 1917.
        Etymology
        Identification
        External Crossreferences and Linkouts ( 47 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        KEGG Genes - Molecular building blocks of life in the genomic space.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (8)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (68)