General Information
Symbol
Dmel\slmb
Species
D. melanogaster
Name
supernumerary limbs
Annotation Symbol
CG3412
Feature Type
FlyBase ID
FBgn0283468
Gene Model Status
Stock Availability
Gene Snapshot
Supernumerary limbs (Slmb) is an essential, conserved F-box protein and a component of the SCF (Skp/Cullin/F-box) E3 ubiquitin-ligase, providing substrate specificity to the SCF. Slmb negatively regulates multiple signaling pathways and cellular processes by promoting the proteasome-mediated degradation or cleavage of its targets, including arm and ci (Wg and Hh signaling), SAK, per and Cap-H2. [Date last reviewed: 2016-09-01]
Also Known As
slimb, ica, shv, wel, MENE(3R)-B
Genomic Location
Cytogenetic map
Sequence location
3R:21,119,252..21,127,485 [-]
Recombination map
3-70
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Experimental Evidence
Predictions / Assertions
Summaries
Gene Group Membership
F BOX AND WD DOMAIN PROTEINS -
F box and WD domain (FBXW) proteins are F box proteins which contain WD40 repeats. F box proteins are the variable substrate adapters for Skp, Cullin, F-box (SCF) E3 ubiquitin ligase complexes. (Adapted from FBrf0225076).
Negative Regulators of Wnt-TCF Signaling Pathway -
Negative regulators of canonical Wnt signaling down-regulation the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
SCF-SLMB UBIQUITIN LIGASE COMPLEX -
SCF-slmb ubiquitin ligase complex ubiquitinates proteins, targeting them for degradation by the proteasome. (FBrf0217546).
Negative Regulators of Insulin-like Receptor Signaling Pathway -
Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
Negative Regulators of Hedgehog Signaling Pathway -
Negative regulators of hedgehog signaling down-regulation the pathway, resulting in the repression of transcription of hh-responsive genes.
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\slmb or the JBrowse view of Dmel\slmb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Stop-codon suppression (UAA) postulated; FBrf0216884.
Gene model reviewed during 5.44
gene_with_stop_codon_read_through ; SO:0000697
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0084032
3492
510
FBtr0330049
3492
597
FBtr0333931
2549
510
Additional Transcript Data and Comments
Reported size (kB)
4.0, 3.0, 2.6 (northern blot)
2.154 (compiled cDNA)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0083434
59.0
510
7.67
FBpp0303082
68.6
597
5.18
FBpp0306059
59.0
510
7.67
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

510 aa isoforms: slmb-PA, slmb-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\slmb using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (38 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:ci; FB:FBgn0004859
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR021977
(assigned by InterPro )
contributes_tocontributes_to ubiquitin-protein transferase activity
inferred from sequence model
inferred from sequence or structural similarity with HGNC:1144
Biological Process (34 terms)
Terms Based on Experimental Evidence (32 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from physical interaction with FLYBASE:dco; FB:FBgn0002413
inferred from physical interaction with FLYBASE:per; FB:FBgn0003068
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Expression Data
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism | ubiquitous

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\slmb in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 36 )
For All Classical and Insertion Alleles Show
 
Allele of slmb
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    Yes
      0
      --
        0
        Yes
        Other relevant insertions
        Transgenic Constructs ( 22 )
        For All Alleles Carried on Transgenic Constructs Show
        Transgenic constructs containing/affecting coding region of slmb
        Allele of slmb
        Mutagen
        Associated Transgenic Construct
        Stocks
        Transgenic constructs containing regulatory region of slmb
        Deletions and Duplications ( 4 )
        Summary of Phenotypes
        For more details about a specific phenotype click on the relevant allele symbol.
        Lethality
        Allele
        Sterility
        Allele
        Other Phenotypes
        Allele
        Phenotype manifest in
        Allele
        adult cuticle & head | dorsal | somatic clone
        centrosome & neuroblast
        germarium region 2b & germline cyst | somatic clone
        germarium region 3 & germline cyst | somatic clone
        macrochaeta & wing margin
        mitotic cell cycle & neuroblast
        Orthologs
        Human Orthologs (via DIOPT v7.1)
        Homo sapiens (Human) (5)
        Species\Gene Symbol
        Score
        Best Score
        Best Reverse Score
        Alignment
        Complementation?
        Transgene?
        11 of 15
        Yes
        Yes
         
        11 of 15
        Yes
        Yes
        1 of 15
        No
        No
        1 of 15
        No
        No
        1 of 15
        No
        Yes
        Model Organism Orthologs (via DIOPT v7.1)
        Mus musculus (laboratory mouse) (4)
        Species\Gene Symbol
        Score
        Best Score
        Best Reverse Score
        Alignment
        Complementation?
        Transgene?
        12 of 15
        Yes
        Yes
        10 of 15
        No
        Yes
        1 of 15
        No
        No
        1 of 15
        No
        No
        Rattus norvegicus (Norway rat) (2)
        10 of 13
        Yes
        Yes
        5 of 13
        No
        Yes
        Xenopus tropicalis (Western clawed frog) (15)
        9 of 12
        Yes
        Yes
        1 of 12
        No
        No
        1 of 12
        No
        No
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        1 of 12
        No
        No
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        1 of 12
        No
        Yes
        Danio rerio (Zebrafish) (5)
        11 of 15
        Yes
        Yes
        11 of 15
        Yes
        Yes
        1 of 15
        No
        No
        1 of 15
        No
        No
        1 of 15
        No
        Yes
        Caenorhabditis elegans (Nematode, roundworm) (2)
        12 of 15
        Yes
        Yes
        1 of 15
        No
        No
        Arabidopsis thaliana (thale-cress) (1)
        1 of 9
        Yes
        Yes
        Saccharomyces cerevisiae (Brewer's yeast) (3)
        4 of 15
        Yes
        Yes
        1 of 15
        No
        No
        1 of 15
        No
        Yes
        Schizosaccharomyces pombe (Fission yeast) (3)
        8 of 12
        Yes
        Yes
        2 of 12
        No
        Yes
        1 of 12
        No
        No
        Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091904T1 )
        Organism
        Common Name
        Gene
        AAA Syntenic Ortholog
        Multiple Dmel Genes in this Orthologous Group
        Drosophila melanogaster
        fruit fly
        Drosophila suzukii
        Spotted wing Drosophila
        Drosophila simulans
        Drosophila sechellia
        Drosophila sechellia
        Drosophila erecta
        Drosophila yakuba
        Drosophila ananassae
        Drosophila pseudoobscura pseudoobscura
        Drosophila persimilis
        Drosophila willistoni
        Drosophila virilis
        Drosophila mojavensis
        Drosophila grimshawi
        Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503O5 )
        Organism
        Common Name
        Gene
        Multiple Dmel Genes in this Orthologous Group
        Musca domestica
        House fly
        Glossina morsitans
        Tsetse fly
        Lucilia cuprina
        Australian sheep blowfly
        Mayetiola destructor
        Hessian fly
        Aedes aegypti
        Yellow fever mosquito
        Anopheles darlingi
        American malaria mosquito
        Anopheles gambiae
        Malaria mosquito
        Culex quinquefasciatus
        Southern house mosquito
        Culex quinquefasciatus
        Southern house mosquito
        Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03GQ )
        Organism
        Common Name
        Gene
        Multiple Dmel Genes in this Orthologous Group
        Bombyx mori
        Silkmoth
        Danaus plexippus
        Monarch butterfly
        Heliconius melpomene
        Postman butterfly
        Apis florea
        Little honeybee
        Apis mellifera
        Western honey bee
        Apis mellifera
        Western honey bee
        Bombus impatiens
        Common eastern bumble bee
        Bombus terrestris
        Buff-tailed bumblebee
        Linepithema humile
        Argentine ant
        Megachile rotundata
        Alfalfa leafcutting bee
        Nasonia vitripennis
        Parasitic wasp
        Dendroctonus ponderosae
        Mountain pine beetle
        Tribolium castaneum
        Red flour beetle
        Pediculus humanus
        Human body louse
        Rhodnius prolixus
        Kissing bug
        Cimex lectularius
        Bed bug
        Acyrthosiphon pisum
        Pea aphid
        Zootermopsis nevadensis
        Nevada dampwood termite
        Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03DK )
        Organism
        Common Name
        Gene
        Multiple Dmel Genes in this Orthologous Group
        Strigamia maritima
        European centipede
        Stegodyphus mimosarum
        African social velvet spider
        Tetranychus urticae
        Two-spotted spider mite
        Tetranychus urticae
        Two-spotted spider mite
        Daphnia pulex
        Water flea
        Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0437 )
        Organism
        Common Name
        Gene
        Multiple Dmel Genes in this Orthologous Group
        Strongylocentrotus purpuratus
        Purple sea urchin
        Ciona intestinalis
        Vase tunicate
        Gallus gallus
        Domestic chicken
        Gallus gallus
        Domestic chicken
        Human Disease Model Data
        FlyBase Human Disease Model Reports
          Alleles Reported to Model Human Disease (Disease Ontology)
          Download
          Models ( 0 )
          Allele
          Disease
          Evidence
          References
          Interactions ( 1 )
          Allele
          Disease
          Interaction
          References
          Comments ( 0 )
           
          Human Orthologs (via DIOPT v7.1)
          Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
          Homo sapiens (Human)
          Gene name
          Score
          OMIM
          OMIM Phenotype
          Complementation?
          Transgene?
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          esyN Network Diagram
          Show neighbor-neighbor interactions:
          Select Layout:
          Legend:
          Protein
          RNA
          Selected Interactor(s)
          Interactions Browser

          Please look at the Interaction Group reports for full details of the physical interactions
          protein-protein
          Interacting group
          Assay
          References
          RNA-protein
          Interacting group
          Assay
          References
          Summary of Genetic Interactions
          esyN Network Diagram
          esyN Network Key:
          Suppression
          Enhancement

          Please look at the allele data for full details of the genetic interactions
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          External Data
          Linkouts
          Pathways
          Gene Group - Pathway Membership (FlyBase)
          Negative Regulators of Wnt-TCF Signaling Pathway -
          Negative regulators of canonical Wnt signaling down-regulation the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
          Negative Regulators of Insulin-like Receptor Signaling Pathway -
          Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
          Negative Regulators of Hedgehog Signaling Pathway -
          Negative regulators of hedgehog signaling down-regulation the pathway, resulting in the repression of transcription of hh-responsive genes.
          External Data
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          3R
          Recombination map
          3-70
          Cytogenetic map
          Sequence location
          3R:21,119,252..21,127,485 [-]
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          93B13-93C1
          Limits computationally determined from genome sequence between P{PZ}Atpα01164 and P{PZ}mod(mdg4)07038&P{lacW}mod(mdg4)L3101
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          93B10-93B11
          93B10-93B11
          (determined by in situ hybridisation)
          93B1-93B11
          (determined by in situ hybridisation)
          62B4-62B5
          68C12--13 93B10--11
          Experimentally Determined Recombination Data
          Left of (cM)
          Right of (cM)
          Stocks and Reagents
          Stocks (28)
          Genomic Clones (22)
          cDNA Clones (124)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequences
          BDGP DGC clones
          Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          RNAi and Array Information
          Linkouts
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for identity of: slmb CG3412
          Source for database merge of
          Source for merge of: slmb CG3412
          Source for merge of: slmb wel
          Source for merge of: slmb BcDNA:GM02031
          Source for merge of: slmb shv
          Source for merge of: slmb ica
          Source for merge of: slmb MENE(3R)-B
          Additional comments
          Source for merge of slmb CG3412 was sequence comparison ( date:000414 ).
          Source for merge of slmb BcDNA:GM02031 was a shared cDNA ( date:030728 ).
          Other Comments
          slmb antagonises the aPKC/par-6 complex to control oocyte and epithelial polarity.
          slmb maintains an S-phase reduplication block by down-regulating SAK on centrioles.
          Processing of ci-155 to the repressor ci-75 is mediated by direct binding to slmb.
          Identified as a component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.
          dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
          Identified in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 2 alleles of wel have been identified in the screen.
          slmb is an essential part of the circadian clock, it controls the levels of clock proteins per and tim.
          slmb is likely to be involved, in addition to its repression role on dpp and wg, in some other essential cellular mechanism that would be essential for maintaining imaginal cells affinities.
          Isolated in an EMS screen for recessive mutations that alter normal adult patterning in somatic clones.
          Loss of slmb function results in a cell-autonomous accumulation of high levels of both ci and arm and ectopic expression of both hh- and wg- responsive genes.
          slmb negatively regulates hh and wg signal transduction.
          Strong alleles cause embryonic lethality while weak alleles cause larval and pupal lethality.
          slmb coordinates wg and dpp expression in the dorsal-ventral and anterior-posterior axes during limb development.
          Isolated in a screen for mutations that modify fu phenotypes.
          Identified in an adult mosaic screen for negative regulators of cell proliferation.
          Origin and Etymology
          Discoverer
          Etymology
          Given the name "pingiel", after one of Pavlov's dogs.
          To reflect the fact that many of the mutants isolated in this screen disrupt Avic\GFP-stau localisation to the anterior and/or posterior pole of the oocyte, many of the complementation groups identified in the screen have been named after polar explorers who failed to reach the North or South pole.
          Identification
          External Crossreferences and Linkouts ( 72 )
          Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Linkouts
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          KEGG Genes - Molecular building blocks of life in the genomic space.
          Synonyms and Secondary IDs (28)
          Reported As
          Symbol Synonym
          BcDNA:GM02031
          MENE (3R)-B
          Slimb/beta-TrCP
          Slimb/β-TRCP
          crd
          l(3)00295
          Secondary FlyBase IDs
          • FBgn0260565
          • FBgn0267841
          • FBgn0023423
          • FBgn0016060
          • FBgn0023437
          • FBgn0063206
          • FBgn0067639
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (277)