FB2025_01 , released February 20, 2025
Gene: Dmel\Alp2
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General Information
Symbol
Dmel\Alp2
Species
D. melanogaster
Name
Alkaline phosphatase 2
Annotation Symbol
CG3290
Feature Type
protein_coding_gene
FlyBase ID
FBgn0283480
Gene Model Status
Current
Stock Availability
4 publicly available
Enzyme Name (EC)
alkaline phosphatase (3.1.3.1)
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
58D1-58D1
Sequence location
Recombination map
2-99
RefSeq locus
NT_033778 REGION:22206005..22207987
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (3 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables alkaline phosphatase activity
inferred from direct assay
(Schneiderman et al., 1966)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables alkaline phosphatase activity
inferred from biological aspect of ancestor with PANTHER:PTN000174527
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with HGNC:439
(Gene Ontology Curators, 2002-)
enables phosphatase activity
inferred from electronic annotation with InterPro:IPR001952, InterPro:IPR018299
(InterPro Project Members, 2004-)
Biological Process (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in cell surface
inferred from sequence or structural similarity with HGNC:439
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
alkaline phosphatase activity
a phosphate monoester + H2O = an alcohol + phosphate (3.1.3.1)
Summaries
Gene Group (FlyBase)
ALKALINE PHOSPHATASES -
Alkaline phosphatases function best in alkaline environments, catalyzing the hydrolysis of monoesters of phosphoric acid and a transphosphorylation reaction in the presence of large concentrations of phosphate acceptors. With few exceptions, they are homodimeric enzymes and each catalytic site contains three metal ions, i.e., two Zn and one Mg, necessary for enzymatic activity. They have broad substrate specificity. (Adapted from PMID:18404473.)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Aph-2
The alkaline phosphatase found in adult hindgut.
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Alp2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W274)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.51

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Alp2-RA
FBtr0071782
NM_137810
1762
538
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Alp2-PA
FBpp0071695
58.2
538
6.11
Q9W274
NP_611654
AAF46820
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Alp2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.24

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Alp2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Alp2
Transgenic constructs containing regulatory region of Alp2
Aberrations (Deficiencies and Duplications) ( 1 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
electrophoretic variant | fast
electrophoretic variant | slow
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
Hsap\ALPG
8 of 14
Yes
No
Hsap\ALPI
7 of 14
No
No
Hsap\ALPL
7 of 14
No
No
1  
Hsap\ALPP
7 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (5)
Rnor\Alpi
8 of 14
Yes
No
Rnor\Alpl
8 of 14
Yes
No
Rnor\Alpp
8 of 14
Yes
No
Rnor\Akp3
7 of 14
No
No
Rnor\Alpg
6 of 14
No
No
Mus musculus (laboratory mouse) (4)
Mmus\Akp3
8 of 14
Yes
No
Mmus\Alpi
8 of 14
Yes
No
Mmus\Alpl
8 of 14
Yes
No
Mmus\Alppl2
8 of 14
Yes
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\alpi.1
7 of 13
Yes
No
Xtro\alpl
7 of 13
Yes
No
Xtro\alpi.2
5 of 13
No
No
Xtro\arnt2
1 of 13
No
No
Danio rerio (Zebrafish) (4)
Drer\alpl
8 of 14
Yes
No
Drer\alp3
7 of 14
No
No
Drer\alpi.1
7 of 14
No
No
Drer\alpi.2
7 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (12)
Agam\AgaP_AGAP001684
6 of 12
Yes
No
Agam\AgaP_AGAP004578
6 of 12
Yes
No
Agam\AgaP_AGAP007300
6 of 12
Yes
No
Agam\AgaP_AGAP010596
6 of 12
Yes
No
Agam\AgaP_AGAP011302
6 of 12
Yes
No
Agam\AgaP_AGAP012634
6 of 12
Yes
No
Agam\AgaP_AGAP006400
5 of 12
No
No
Agam\AgaP_AGAP011303
5 of 12
No
Yes
Agam\AgaP_AGAP011305
5 of 12
No
No
Agam\AgaP_AGAP011202
3 of 12
No
No
Agam\AgaP_AGAP011304
3 of 12
No
Yes
Agam\AgaP_AGAP012589
3 of 12
No
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\PHO8
10 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPBC14F5.13c
9 of 12
Yes
No
Escherichia coli (enterobacterium) (1)
Ecol\phoA
9 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Alp2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (12)
Alp7
11 of 13
Alp8
11 of 13
Alp1
10 of 13
Alp4
10 of 13
Alp5
10 of 13
Alp6
10 of 13
Alp9
10 of 13
Alp10
10 of 13
Alp11
10 of 13
Alp12
10 of 13
phu
10 of 13
Alp13
6 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ALPG; alkaline phosphatase, germ cell
    8 of 14
    171810
        ALPI; alkaline phosphatase, intestinal
        7 of 14
        171740
            ALPL; alkaline phosphatase, biomineralization associated
            7 of 14
            171760
             
            ALPP; alkaline phosphatase, placental
            7 of 14
            171800
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                esyN Network Diagram
                Other Interaction Browsers
                Summary of Genetic Interactions
                esyN Network Diagram
                Other Interaction Browsers
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                External Data
                Linkouts
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyBase
                External Links
                KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
                External Data
                Linkouts
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Class of Gene
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                2R
                Recombination map
                2-99
                Cytogenetic map
                58D1-58D1
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                58D1-58D1
                Limits computationally determined from genome sequence between P{EP}CG6393EP2457 and P{PZ}ari-207768
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                Experimentally Determined Recombination Data
                Location

                2-99

                (FlyBase, 2016)

                2-103.8

                (Comeron et al., 2012)

                2-

                Left of (cM)
                Right of (cM)
                Notes

                Not mapped within chromosome 2.

                Stocks and Reagents
                Stocks (4)
                Genomic Clones (17)
                 

                Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                cDNA Clones (4)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                  Other clones
                  RNAi and Array Information
                  Linkouts
                  Antibody Information
                  Laboratory Generated Antibodies
                   
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                      Other Comments

                      The alkaline phosphatase found in adult hindgut.

                      Relationship to Other Genes
                      Source for database merge of

                      Source for merge of: Aph-2 CG3290

                      (Marygold, 2015.6.26)
                      Additional comments

                      'Aph-2' is only mentioned in a single publication (FBrf0018140). Therein, it's mapped to the second chromosome and is found to be expressed in the adult hindgut, persisting at high levels throughout adulthood. Of the 13 annotated Dmel genes predicted to encoded Alkaline phosphatases, only CG3290 meets these criteria (adult hindgut expression supported by FlyAtlas, modENCODE and Flygut data).

                      (Marygold, 2015.6.26)

                      The genes CG3264 and CG3290 share >70% identity and close proximity, and so may have been derived from a DNA-based tandem duplication.

                      (Langille and Clark, 2007)
                      Nomenclature History
                      Source for database identify of

                      Source for identity of: Alp2 Aph-2

                      (Marygold, 2015.6.26)
                      Nomenclature comments
                      Etymology
                      Synonyms and Secondary IDs (4)
                      Datasets (0)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (0)
                      Result
                      Result Type
                      Title
                      External Crossreferences and Linkouts ( 37 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                      Other crossreferences
                      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      InterPro - A database of protein families, domains and functional sites
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      Linkouts
                      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                      KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                      Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
                      References (46)