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General Information
Symbol
Dmel\lola
Species
D. melanogaster
Name
longitudinals lacking
Annotation Symbol
CG12052
Feature Type
FlyBase ID
FBgn0283521
Gene Model Status
Stock Availability
Gene Snapshot
longitudinals lacking (lola) encodes a protein involved in Notch signaling, cell death, regulation of retrotransposons and expression of axon and dendrite patterning genes. It contributes to oogenesis, spermatogenesis, neural wiring, eye development and a variety of behaviors. [Date last reviewed: 2019-03-21]
Also Known As

eyeful, longitudinal lacking, btb, misguided, BTB-IV

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:10,481,894..10,543,291 [-]
Recombination map

2-62

RefSeq locus
NT_033778 REGION:10481894..10543291
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
Predictions / Assertions
Summaries
Gene Group (FlyBase)
C2H2 ZINC FINGER TRANSCRIPTION FACTORS -
Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys2His2 zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).
Pathway (FlyBase)
Positive Regulators of Imd Signaling Pathway -
Positive regulators of the immune deficiency (Imd) pathway result in the increased activity of the NFκB-like transcription factor Rel in the nucleus. (Adapted from FBrf0224587 and FBrf0238555.)
Protein Function (UniProtKB)
Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.
Summary (Interactive Fly)

transcription factor - zinc finger - btb domain - regulates axon guidance - promotes axon growth in part by suppressing expression of the actin nucleation factor Spire - expressed in both glia and neurons - regulates cell fate by antagonizing Notch induction in the Drosophila eye

Gene Model and Products
Number of Transcripts
25
Number of Unique Polypeptides
20

Please see the GBrowse view of Dmel\lola or the JBrowse view of Dmel\lola for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

gene_with_trans_spliced_transcript ; SO:0000459

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0089344
2902
706
FBtr0343132
4623
970
FBtr0089343
2974
787
FBtr0089345
3123
787
FBtr0089349
3423
748
FBtr0089342
3417
748
FBtr0089348
2627
565
FBtr0089347
4267
891
FBtr0089346
2146
518
FBtr0089350
4239
970
FBtr0089360
3703
757
FBtr0089353
2055
546
FBtr0089352
2823
608
FBtr0089362
2150
465
FBtr0089361
3234
668
FBtr0089355
3875
963
FBtr0089356
2327
603
FBtr0089357
4015
891
FBtr0089359
2245
575
FBtr0089351
2239
575
FBtr0089363
2438
549
FBtr0089364
3446
771
FBtr0089365
2938
602
FBtr0089366
2397
577
FBtr0100286
2707
665
Additional Transcript Data and Comments
Reported size (kB)

3.9 (northern blot)

4.9, 4.7, 3.8 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0088378
75.8
706
5.15
FBpp0309831
105.8
970
6.11
FBpp0088377
82.2
787
6.14
FBpp0088379
82.2
787
6.14
FBpp0088383
79.4
748
5.48
FBpp0088376
79.4
748
5.48
FBpp0088382
60.7
565
6.09
FBpp0088381
96.3
891
6.43
FBpp0088380
55.4
518
4.97
FBpp0088384
105.8
970
6.11
FBpp0088394
78.7
757
6.63
FBpp0088387
58.9
546
6.27
FBpp0088386
65.4
608
6.61
FBpp0088396
49.3
465
4.80
FBpp0088395
71.8
668
4.64
FBpp0088389
102.7
963
5.14
FBpp0088390
64.7
603
8.12
FBpp0088391
96.3
891
6.43
FBpp0088393
61.9
575
6.21
FBpp0088385
61.9
575
6.21
FBpp0088949
59.3
549
5.20
FBpp0088950
83.5
771
5.66
FBpp0088951
64.4
602
5.84
FBpp0088948
62.7
577
5.19
FBpp0099680
70.9
665
6.60
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

970 aa isoforms: lola-PAA, lola-PI
891 aa isoforms: lola-PG, lola-PR
575 aa isoforms: lola-PT, lola-PU
748 aa isoforms: lola-PD, lola-PE
787 aa isoforms: lola-PB, lola-PC
Additional Polypeptide Data and Comments
Reported size (kDa)

748 (aa); 105 (kD)

894, 467 (aa); 97, 50 (kD predicted)

Comments

Although the calculated mass of the zf5 lola isoform is79.4 kD, it migrates at 105 kD perhaps because of its low pI.

External Data
Subunit Structure (UniProtKB)

Isoform D interacts with JIL-1.

(UniProt, Q7KQZ4)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lola using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (24 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from physical interaction with FLYBASE:JIL-1; FB:FBgn0020412
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with FLYBASE:ttk; FB:FBgn0003870
Biological Process (18 terms)
Terms Based on Experimental Evidence (16 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:SoxN; FB:FBgn0029123
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:robo1; FB:FBgn0005631
inferred from genetic interaction with FLYBASE:sli; FB:FBgn0264089
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from expression pattern
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001135010
(assigned by GO_Central )
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001135010
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
mesoderm

Comment: lola probe L (lola-RB, lola-RC)

amnioproctodeal invagination

Comment: lola probe L (lola-RB, lola-RC)

cell of ectoderm

Comment: lola probe J (lola-RU)

embryonic somatic muscle

Comment: lola probe L (lola-RB, lola-RC)

embryonic epidermis

Comment: lola probe K (lola-RI)

ventral furrow

Comment: lola probe N (lola-RP)

tracheal pit

Comment: lola probe J (lola-RU)

cell | subset of ventral neurectoderm

Comment: lola probe C (lola-RL)

presumptive embryonic/larval central nervous system | restricted

Comment: lola probe I (lola-RF); enriched in dorsal cell layer of embryonic CNS

organism

Comment: maternally deposited

primary spermatocyte

Comment: probe specific to lola-RB, lola-RC, and lola-RI

germinal proliferation center

Comment: probe specific to lola-RB, lola-RC, and lola-RI

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: 0-22 hr AEL

organism
nucleus

Comment: 0-22 hr AEL; different lola isoforms have different temporal distributions

Additional Descriptive Data

lola is expressed in all neurons adjacent to the antennal lobe at all developmental stages of development from larva to adult.

Multiple isoforms ofthe lola protein are detected throughout development. In late embryos up to 15 distinct bands can be detected on western blots using an antibody raised to the core BTB/Poz domain shared by all the predicted isoforms.

lola protein distribution (as determined by an antibody that recognizes both protein forms) is consistent with lola transcript distribution but reveals new information. During gastrulation, the protein appears to turn over at mitosis. It is present at highest concentrations in neurons at stage 13 but is also present at substantial levels in epithelial cells and in sensory organ support cells. Unlike the RNA, lola protein is detected in mesodermal nuclei as late as stage 17. It is observed in somatic muscle and in peritracheal cells.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\lola in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 207 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 39 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of lola
Transgenic constructs containing regulatory region of lola
Deletions and Duplications ( 15 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
axon & adult antennal lobe projection neuron | somatic clone
axon & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146
axon & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lola4.7.UAS
axon & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lolae76
axon & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lolaL.UAS
axon & adult antennal lobe projection neuron | somatic clone | heat sensitive, with Scer\GAL4GH146
axon & larval antennal lobe projection neuron | somatic clone | penetrance incomplete | expressivity incomplete | cell autonomous
dendrite & adult antennal lobe projection neuron | somatic clone
dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146
dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lola3.8.UAS
dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lola4.7.UAS
dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lolae76
dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lolaL.UAS
dendrite & adult antennal lobe projection neuron | somatic clone | heat sensitive, with Scer\GAL4GH146
dendrite & larval antennal lobe projection neuron | somatic clone | penetrance incomplete | expressivity incomplete | cell autonomous
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
1 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
1 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
1 of 13
Yes
No
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (1)
1 of 15
Yes
No
Caenorhabditis elegans (Nematode, roundworm) (1)
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (2)
1 of 15
Yes
No
1 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919048Y )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502DI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W024W )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0L95 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ixodes scapularis
Black-legged tick
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0WNB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (23)
4 of 10
4 of 10
4 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
RNA-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Isoform D interacts with JIL-1.
(UniProt, Q7KQZ4 )
Linkouts
BioGRID - A database of protein and genetic interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Positive Regulators of Imd Signaling Pathway -
Positive regulators of the immune deficiency (Imd) pathway result in the increased activity of the NFκB-like transcription factor Rel in the nucleus. (Adapted from FBrf0224587 and FBrf0238555.)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map

2-62

Cytogenetic map
Sequence location
2R:10,481,894..10,543,291 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
47A-47B
Left limit from in situ hybridisation (FBrf0080145) Right limit from in situ hybridisation (FBrf0080145)
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
47A-47B14
(determined by in situ hybridisation)
Location based on in situ hybridisation using flanking plasmid rescue sequence from the P{lacW}btbk09901 insertion.
47A13-47A13
(determined by in situ hybridisation)
47A11-47A12
(determined by in situ hybridisation)
47A13-47A16
(determined by in situ hybridisation)
47A-47B
(determined by in situ hybridisation)
47A9-47A14
(determined by in situ hybridisation)
47A11-47A14
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (72)
Genomic Clones (32)
cDNA Clones (722)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
Other clones
RNAi and Array Information
Linkouts
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
Other Information
Relationship to Other Genes
Source for database identify of
Source for database merge of

Source for merge of: lola BcDNA:RH31485

Source for merge of: lola BtbIV

Source for merge of: lola l(2)s3697

Source for merge of: lola CG12052 CG18376

Source for merge of: lola sw59 BEST:LD03274

Source for merge of: lola NEST:bs06a08

Source for merge of: lola CG18378 CG18379 CG18380 CG18381

Source for merge of: lola btb

Additional comments

btb[k09901] shows nervous system defects similar to alleles of lola. It also fails to complement several lola alleles, including lola00642, lola5D2, lolaORC4, lolaORE50, lola4E4 and lolaORE120. Previous papers (FBrf0131381) have shown that btb[k09901] complements other alleles of lola, however, lola alleles are known to show intragenic complementation, which can be explained by the trans-splicing that occurs at the lola locus (FBrf0167601). Thus, overall the available evidence indicates that btb[k09901] is an allele of lola.

Source for merge of: lola BcDNA:LD17006

Annotations CG18376, CG18381, CG18380, CG18379, CG18378 and CG12052 merged as CG12052 in release 3 of the genome annotation.

Source for merge of lola BtbIV was sequence comparison ( date:000202 ).

Source for merge of lola BcDNA:LD17006 was a shared cDNA ( date:030728 ).

The insertion has consistently been shown to map to 47A--47B by in situ hybridization (FBrf0080145, FBrf0131381), consistent with the location of lola (47A). However, the flanking sequences reported in FBrf0131381 (AF175907 and AF175908) map to 94A. Sequencing of the line by the GDP in 2009 (GSS sequences KS308221 and KS308220, reported in FBrf0230166) places the insertion at 2R:10534369 (minus strand) (genome release 6 coordinates). This is within the lola gene, consistent with the in situ localization data and supporting the hypothesis of FBrf0194763 that l(2)k09901 is a lola allele.

The available evidence therefore suggests that the l(2)k09901 line is an allele of lola. Thus the 'btb, bumper-to-bumper' gene (of which l(2)k09901 is the only allele) has been merged with the lola gene and the btb[k09901] allele has been renamed lola[k09901] in FlyBase (date: 2015.10.22).

There have been conflicting results in the literature regarding the location and allelism of the l(2)k09901 insertion line.

FBrf0194763 hypothesised that the l(2)k09901 insertion line is an allele of lola, based on its failure to complement several alleles of lola. In contrast, FBrf0080145 and FBrf0131381 report complementation between l(2)k09901 and lola alleles. As discussed in FBrf0194763, intragenic complementation between other lola alleles has been seen previously (FBrf0167601) and can be explained by the trans-splicing that occurs at the lola locus (FBrf0167601), thus reconciling these apparently conflicting complementation results.

Other Comments

lola is required to regulate stem cell differentiation in type II neural stem cells in the larval central brain.

ChEST reveals this is a target of Mef2.

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a specific decrease in AttA activity in response to heat-killed E.coli when assayed in S2 cells.

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

lola mRNAs are generated by alternative trans-splicing.

Identification: as a suppressor of the small eye phenotype caused by overexpression of fng in the eye.

'eyeful' mutant interacts genetically with N and fng though it is not yet clear whether 'eyeful' corresponds to lola or psq.

Five EMS induced alleles have been identified in a screen for mutations affecting commissure formation in the CNS of the embryo.

Homozygous germline clones have not been recovered. lola may perform some essential function in the germline during oogenesis.

lola is required for required for pathfinding and targeting of the SNb motor nerve. lola is a dose dependant regulator of SNb development.

Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.

Dhyd\lola-like is structurally related to lola.

lola encodes a transcription factor required for axon growth and guidance in the embryo.

In lola mutant embryos growth cones that normally pioneer longitudinal pathways initially extend but then stall, thereby lacking most longitudinal axon pathways.

Mutations lack longitudinal connectives of embryonic ventral nerve cord.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 374 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
KEGG Genes - Molecular building blocks of life in the genomic space.
Linkouts
BioGRID - A database of protein and genetic interactions.
Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (36)
Reported As
Symbol Synonym
BEST:LD03274
BcDNA:LD17006
BcDNA:RH31485
BtbIV
CG18376
CG18380
CG30013
CG30014
NEST:bs06a08
bs06a08.y1
lola
(Camus et al., 2019, Chai et al., 2019, Hope et al., 2019, Rivera et al., 2019, Sato et al., 2019, Shokri et al., 2019, Bischof et al., 2018, Katanaev et al., 2018, Lee et al., 2018, Tokusumi et al., 2018, Troha et al., 2018, Ugrankar et al., 2018, Bonneaud et al., 2017, Dinges et al., 2017, Hu et al., 2017.6.13, Alavi et al., 2016, Chow et al., 2016, Froldi and Cheng, 2016, Gene Disruption Project members, 2016-, Hunter et al., 2016, Kockel et al., 2016, Mishra et al., 2016, Peng et al., 2016, Quijano et al., 2016, Silva et al., 2016, Angrisani et al., 2015, Dorn and Dorn, 2015, Gao et al., 2015, Kavi et al., 2015, Nadimpalli et al., 2015, Ríos-Barrera et al., 2015, Schertel et al., 2015, Ugrankar et al., 2015, Zhang et al., 2015, Ashwal-Fluss et al., 2014, Bonnay et al., 2014, Boyle et al., 2014, Chen et al., 2014, Dobi et al., 2014, Fedotov et al., 2014, Ferreira et al., 2014, Mondal et al., 2014, Neville et al., 2014, Slattery et al., 2014, Southall et al., 2014, Tripathy et al., 2014, Zarin et al., 2014, Brown et al., 2013, Daneshvar et al., 2013, Davies et al., 2013, Djiane et al., 2013, Enuameh et al., 2013, Hattori et al., 2013, Iyer et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Schertel et al., 2013, Shen et al., 2013, Spokony and White, 2013.5.10, Wang et al., 2013, Aoki et al., 2012, Bartoletti et al., 2012, Berger et al., 2012, Fukui et al., 2012, Garcia et al., 2012, Hadar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Popkova et al., 2012, Rodriguez et al., 2012, Spokony and White, 2012.5.22, Takahashi et al., 2012, Zhai et al., 2012, Abruzzi et al., 2011, Bonchuk et al., 2011, Friedman et al., 2011, Graveley et al., 2011, Grimberg et al., 2011, Jayanandanan et al., 2011, Kazemian et al., 2011, Miles et al., 2011, Neumüller et al., 2011, Sameny and Locke, 2011, Seong et al., 2011, Toku et al., 2011, Vallejo et al., 2011, Waldholm et al., 2011, Weyers et al., 2011, Zheng et al., 2011, Baig et al., 2010, Blanco et al., 2010, Buffin and Gho, 2010, Cunha et al., 2010, Foley et al., 2010, Gan et al., 2010, Kim and Marqués, 2010, McManus et al., 2010, Potter et al., 2010, Rouault et al., 2010, Silicheva et al., 2010, Ayroles et al., 2009, Bossuyt et al., 2009, Carreira et al., 2009, Krejcí et al., 2009, Moran and Kyriacou, 2009, Otte et al., 2009, Shalaby et al., 2009, Southall and Brand, 2009, Tsubouchi et al., 2009, Wheeler et al., 2009, Yamamoto et al., 2009, Christensen et al., 2008.9.3, Strub et al., 2008, Yamamoto et al., 2008, Zheng and Carthew, 2008, Bass and McCall, 2007, Bass et al., 2007, Buszczak et al., 2007, Gregory et al., 2007, Magalhaes et al., 2007, Maines et al., 2007, Minidorff et al., 2007, Morozova et al., 2007, Mukai et al., 2007, Nurminsky, 2007, Quinones-Coello, 2007, Spletter et al., 2007, Spletter et al., 2007, Spokony and Restifo, 2007, Axelson, 2006, Dorus et al., 2006, Edwards et al., 2006, Haynes et al., 2006, Morozova et al., 2006, Zhang et al., 2006, Laviolette et al., 2005, Dimitri et al., 2003, Goeke et al., 2003, Klebes et al., 2002)
sw59
Secondary FlyBase IDs
  • FBgn0005630
  • FBgn0010468
  • FBgn0010691
  • FBgn0012046
  • FBgn0020247
  • FBgn0026589
  • FBgn0033530
  • FBgn0033531
  • FBgn0033532
  • FBgn0033533
  • FBgn0033534
  • FBgn0033535
  • FBgn0045396
  • FBgn0045853
  • FBgn0050012
  • FBgn0050013
  • FBgn0050014
  • FBgn0063156
  • FBgn0064821
  • FBgn0015368
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (329)