l(1)G0054, BEST:GH17330 , BcDNA:GH07910 , EP1413
Gene model reviewed during 5.55
3' UTR contains an ORF that is conserved among close Drosophila species (FBrf0202906; potential peptide 129 aa in length).
Due to significant sequence change in this region between Releases 4 and 5, data that predate Release 5 are not shown for some categories of evidence.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Stop-codon suppression (UAG) postulated; FBrf0243886.
Gene model reviewed during 6.32
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tlk using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Tlk in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Tlk tlk
Source for merge of: tlk anon-WO03040301.114 anon-WO03040301.116
Source for merge of: tlk CG32781
Source for merge of: CG12462 EP1413
Source for merge of: CG32782 CG33219
Source for merge of: tlk CG12462
Source for merge of: tlk CG32782
Source for merge of: Tlk EP1408
Annotations CG32782 and CG32781 merged as CG34412 in release 5.2 of the genome annotation.
Sequences corresponding to annotation CG12462 (which was eliminated in release 5.1 of the genome annotation) have been reinstated as part of the CG34412 annotation (which corresponds to tlk) in release 5.2 of the genome annotation.
"l(1)G0299" may affect "CG2829". "l(1)G0167" may affect "CG2829". "l(1)G0343" may affect "CG2829". "l(1)G0054" may affect "CG2829".
Source for merge of tlk anon-WO03040301.114 anon-WO03040301.116 was sequence comparison ( date:051113 ).
Source for merge of tlk CG32782 was sequence comparison ( date:050324 ).
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes decreased γ-tubulin staining at the spindle pole and misalignment of chromosomes along the spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
Annotation CG12462 eliminated in release 5.1 of the genome annotation.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, G2/M+, an increase in the proportion of G2/M phase cells, a decrease in cytokinetic index and a decrease in the mitotic index are seen.
New annotation (CG33219) in release 3.2 of the genome annotation.
The release 4 CG32782 annotation contains sequences corresponding to release 2 annotation CG2829. The release 3 version of CG32782 was incomplete and was missing most of the sequence corresponding to release 2 annotation CG2829 (possibly due to an assembly error), but this has been fixed in release 4 and the release 4 CG32782 annotation now includes all of the release 2 CG2829 sequence. 'CG33219' does not appear in Release 4. The region it was located in in Release 3.2 is now a large TE. 'CG33219' is homologous to annotation CG32782; presumably that is where this little bit of mislaid 'CG33219' sequence originated.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cells become round and detached.
New annotation (CG32782) in release 3 of the genome annotation. Annotation CG2829 was eliminated in release 3 of the genome annotation, October 2002.
New annotation (CG32781) in release 3 of the genome annotation.