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General Information
Symbol
Dmel\eEF1α1
Species
D. melanogaster
Name
eukaryotic translation elongation factor 1 alpha 1
Annotation Symbol
CG8280
Feature Type
FlyBase ID
FBgn0284245
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
Ef1alpha48D, EF-1α, Ef1α48D, EF1α, l(2)00248
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:11,891,135..11,895,762 [+]
Recombination map
2-65
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily. (P08736)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
Summaries
Gene Group (FlyBase)
CYTOPLASMIC TRANSLATION ELONGATION FACTORS -
Cytoplasmic (or eukaryotic) translation elongation factors function in the chain elongation stage of ribosome-mediated polypeptide synthesis in the cytoplasm. (Adapted from FBrf0233552).
Protein Function (UniProtKB)
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
(UniProt, P08736)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Ef1α1: Elongation factor 1α #1
Encodes an α unit of the heterotrimeric protein, elongation factor 1, which promotes the binding of aminoacyl-tRNA to ribosomes. Originally isolated on the basis of its displaying 5 to 10 fold overexpression in females versus males. Ef1α1 is expressed throughout development. The rate of protein synthesis in Drosophila declines with age; this has been attributed to impaired binding of aminoacyl-tRNA to ribosomes, which is promoted by elongation- factor 1 (EF1); transcription of elongation factor-1 mRNA shown to decrease with age; by fifteen days following emergence the level of EF1 mRNA is but a few percent that of newly emerged adults (Webster and Webster, 1984, Mech. Ageing Dev. 24: 335-42). Transformation with Ef1α1 under the control of heat shock promoter increased the mean life span from 38.2 to 45.1 days at 25 and from 21.1 to 29.8 days at 29 (Shepherd et al.).
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\eEF1α1 or the JBrowse view of Dmel\eEF1α1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.45
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0088035
2029
463
FBtr0331927
2278
463
FBtr0345022
1979
463
FBtr0345023
2076
463
Additional Transcript Data and Comments
Reported size (kB)
2.0 (unknown)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0087142
50.3
463
9.60
FBpp0304260
50.3
463
9.60
FBpp0311272
50.3
463
9.60
FBpp0311273
50.3
463
9.60
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eEF1α1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (6 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000795, InterPro:IPR004161, InterPro:IPR004539
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR000795
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR004539
(assigned by InterPro )
inferred from sequence or structural similarity
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR004539
(assigned by InterPro )
inferred from sequence or structural similarity
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
dot blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Ef1α48D transcript is expressed in all developmental stages. Expression in females is 5 to 10-fold higher than in males.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Isolated from a pullout assay for proteins contained in clots from larval hemolymph and identified by mass spec.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eEF1α1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eEF1α1
Transgenic constructs containing regulatory region of eEF1α1
Deletions and Duplications ( 32 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
9 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
9 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
10 of 13
Yes
No
8 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
9 of 12
Yes
Yes
9 of 12
Yes
Yes
7 of 12
No
No
5 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (9)
11 of 15
Yes
Yes
10 of 15
No
Yes
9 of 15
No
Yes
8 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (5)
12 of 15
Yes
Yes
6 of 15
No
Yes
3 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (6)
5 of 9
Yes
Yes
3 of 9
No
Yes
2 of 9
No
Yes
2 of 9
No
Yes
1 of 9
No
No
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (5)
13 of 15
Yes
Yes
8 of 15
No
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (5)
11 of 12
Yes
Yes
10 of 12
No
Yes
6 of 12
No
Yes
3 of 12
No
No
1 of 12
No
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091907AL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915047V )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W040L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0405 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G05LW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (3)
8 of 10
2 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-65
    Cytogenetic map
    Sequence location
    2R:11,891,135..11,895,762 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    48C5-50D7
    Limits computationally determined from genome sequence between P{EP}walEP825&P{PZ}wal02516 and P{PZ}Ef1α48D01275; Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    48C5-48C6
    (determined by in situ hybridisation)
    50D5-50D7
    (determined by in situ hybridisation)
    48C1-50D7
    48C5--6 48C6--8 48C7--8 48D1--2
    48D-48D
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Notes
    Determined by deletion mapping.
    Stocks and Reagents
    Stocks (18)
    Genomic Clones (10)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (2001)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: eEF1α1 Ef1α48D
      Source for database merge of
      Source for merge of: Ef1α48D l(2)k07303
      Source for merge of: Ef1α48D l(2)00248
      Additional comments
      The Ef1α48D gene may have been derived from the Ef1α100E gene by retroposition.
      The Ef1α48D gene may be derived from the Ef1α100E gene by retroposition.
      "l(2)00248" probably corresponds to "Ef1α48D"; the P{PZ} insertion in the "l(2)0024800248" allele maps only 42bp upstream of the transcription start of the CT24517 transcript of "Ef1α48D".
      Not allelic to shot.
      Other Comments
      Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
      In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
      Expression of transcript changes with adult age.
      Expression of Ef1α100E or Ef1α48D is not associated with an extended longevity phenotype.
      The report (FBrf0050863) that expression of Ef1α48D correlates with longevity cannot be confirmed by a study of the expression of this gene and of Ef1α100E in lines with extended longevity.
      An important aging control may be exerted at the level of Ef1α48D transcription but heat induced expression of Ef1α48D does not cause increased levels of Ef1α48D mRNA or protein.
      Enhanced expression of Ef1α48D increases the lifespan of mated females and makes local changes within the bounds of tradeoffs that are given by a pre-existing physiological structure whose basic nature is not changed by the treatment.
      Ef1α48D is the housekeeping gene that is needed in all growing cells. Individuals transformed with a P-element containing Ef1α48D sequences under heat shock control have a longer lifespan than wild type flies.
      Encodes an α unit of the heterotrimeric protein, elongation factor 1, which promotes the binding of aminoacyl-tRNA to ribosomes. Originally isolated on the basis of its displaying 5 to 10 fold overexpression in females versus males. Ef1α1 is expressed throughout development. The rate of protein synthesis in Drosophila declines with age; this has been attributed to impaired binding of aminoacyl-tRNA to ribosomes, which is promoted by elongation-factor 1 (EF1); transcription of elongation factor-1 mRNA shown to decrease with age; by fifteen days following emergence the level of EF1 mRNA is but a few percent that of newly emerged adults (Webster and Webster, 1984). Transformation with Ef1α1 under the control of heat shock promoter increased the mean life span from 38.2 to 45.1 days at 25oC and from 21.1 to 29.8 days at 29oC (Shepherd, Walldorf, Hug and Gehring, 1989).
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 63 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      KEGG Genes - Molecular building blocks of life in the genomic space.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (58)
      Reported As
      Symbol Synonym
      EF1-α
      EF1α 48D
      Ef1alpha 48D
      Ef1alpha48E
      Ef1α1
      anon-EST:Liang-1.17
      anon-EST:Liang-2.46
      chr2R:7409490..7409690
      chr2R:7410009..7410129
      eEF1α
      l(2)01275
      Name Synonyms
      Elongation factor 1 alpha
      Elongation factor 1 alpha 48D
      Elongation factor 1alpha48D
      Elongation factor 1α48D
      Translational elongation factor1
      elongation factor 1-alpha F1
      elongation factor 1alpha48D
      eukaryotic translation elongation factor 1 alpha 1
      lethal (2) 00248
      Secondary FlyBase IDs
      • FBgn0000556
      • FBgn0010480
      • FBgn0022092
      • FBgn0010459
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (172)