FlyBase Gene Report: Dmel\Adf1

MADF-BESS DOMAIN TRANSCRIPTION FACTORS -: The MADF-BESS (Myb-SANT like in Adf) domain transcription factors are sequence-specific DNA binding proteins that regulate transcription. The MADF domain binds to multiple trinucleotide repeats and is often present in combination with a BESS domain that mediates protein-protein interactions. (Adapted from FBrf0155780 and FBrf0224045).

Protein Function (UniProtKB)

May play an important role not only in the regulation of Adh expression but also in the transcription of other genes.

(UniProt, P05552)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Adf1 or the JBrowse view of Dmel\Adf1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.48

Gene model reviewed during 5.44

gene_with_stop_codon_read_through ; SO:0000697

Stop-codon suppression (UAA) postulated; FBrf0216884

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Adf1-RA

FBtr0086111

NM_165479

1668

253

Adf1-RB

FBtr0086112

NM_165480

1795

253

Adf1-RC

FBtr0086113

NM_206028

1675

262

Adf1-RE

FBtr0330252

NM_001273801

1668

274

Additional Transcript Data and Comments

Reported size (kB)

1.9 (northern blot)

(England et al., 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Adf1-PA

FBpp0085446

29.2

253

8.74

NP_724481

AAF57369

Adf1-PB

FBpp0085447

29.2

253

8.74

NP_724482

AAF57370

Adf1-PC

FBpp0089262

30.2

262

8.44

NP_995750

AAS64778

Adf1-PE

FBpp0303284

31.5

274

8.58

NP_001260730

AGB93263

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

253 aa isoforms: Adf1-PA, Adf1-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

253 (aa); 29 (kD)

(England et al., 1992)

Comments

External Data

Post Translational Modification

O-glycosylated; contains N-acetylglucosamine side chains.

(UniProt, P05552)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Adf1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (14 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

DNA-binding transcription activator activity, RNA polymerase II-specific

inferred from direct assay

(Heberlein et al., 1985)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

DNA binding

inferred from electronic annotation with InterPro:IPR004210

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

memory

inferred from mutant phenotype

(DeZazzo et al., 2000)

positive regulation of transcription by RNA polymerase II

inferred from direct assay

(Heberlein et al., 1985)

regulation of dendrite development

inferred from mutant phenotype

(Timmerman et al., 2013)

synapse assembly

inferred from mutant phenotype

(DeZazzo et al., 2000)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

larval locomotory behavior

non-traceable author statement

(Sokolowski, 2001)

learning or memory

non-traceable author statement

(Sokolowski, 2001)

long-term memory

traceable author statement

(Burrell and Sahley, 2001)

olfactory learning

non-traceable author statement

(Roman and Davis, 2001)

regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000956409

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

nucleus

inferred from direct assay

(DeZazzo et al., 2000, England et al., 1992, Heberlein et al., 1985)

polytene chromosome interband

inferred from direct assay

(Berkaeva et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000956409

(assigned by GO_Central )

(Gaudet et al., 2011)

transcription factor complex

inferred from biological aspect of ancestor with PANTHER:PTN000956409

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 7 -- 8

organism | intense | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | intense | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10 -- 17

organism | ubiquitous

Comment: reference states >4 hr AEL

(England et al., 1992)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval brain

(Timmerman et al., 2013)

adult stage

adult brain

(Timmerman et al., 2013)

Additional Descriptive Data

Adf1 is expressed at nearly constant levels throughout the embryo from around 4 or 5 hours after fertilizaton onward. Expression is seen in the nuclei of nearly all cells with the exception of the pole cells.

(England et al., 1992)

Marker for

Subcellular Localization

CV Term

Evidence

References

nucleus

inferred from direct assay

(DeZazzo et al., 2000, England et al., 1992, Heberlein et al., 1985)

polytene chromosome interband

inferred from direct assay

(Berkaeva et al., 2009)

Expression Deduced from Reporters

Reporter: P{GawB}Adf1^GAL4

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval motor neuron | subset of ventral nerve cord

(Timmerman et al., 2013)

ventral nerve cord | restricted

(Timmerman et al., 2013)

RP2 motor neuron

(Timmerman et al., 2013)

aCC neuron

(Timmerman et al., 2013)

adult stage

motor neuron | subset of thoracico-abdominal ganglion

(Timmerman et al., 2013)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Adf1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Adf1

View exonic expression by tissue for Dmel\Adf1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG15845-RA

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 23 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Adf1

Transgenic constructs containing regulatory region of Adf1

Deletions and Duplications ( 14 )

Disrupted in

Df(2R)42

(DeZazzo et al., 2000, BDGP Project Members, 1994-1999)

Df(2R)BSC326

(Kahsai et al., 2016)

Df(2R)cn88b

(BDGP Project Members, 1994-1999)

Df(2R)ED1552

(Kahsai et al., 2016)

Df(2R)hfl-37

(Rendina et al., 2010)

Df(2R)nap2

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)nap12

(DeZazzo et al., 2000)

Df(2R)nap16

(BDGP Project Members, 1994-1999)

Not disrupted in

Df(2R)42

(BDGP Project Members, 1994-1999)

Df(2R)cn87e

(DeZazzo et al., 2000)

Df(2R)cn88b

(DeZazzo et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)nap1

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)nap5

(BDGP Project Members, 1994-1999)

Df(2R)pk78s

(BDGP Project Members, 1994-1999)

In(2LR)TE35B-4^LTE35B-14^R

(BDGP Project Members, 1994-1999)

In(2LR)TE35B-14^LTE35B-4^R

(BDGP Project Members, 1994-1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Adf1^CB02276

lethal, with Scer\GAL4^238Y

Adf1^UAS.cDa

lethal, with Scer\GAL4^C321c

Adf1^UAS.cDa

lethal, with Scer\GAL4^c747

Adf1^UAS.cDa

lethal, with Scer\GAL4^ey-OK107

Adf1^UAS.cDa

lethal, with Scer\GAL4^MZ1580

Adf1^UAS.cDa

lethal, with Scer\GAL4^OK348

Adf1^UAS.cDa

lethal, with Scer\GAL4^Tab2-201Y

Adf1^UAS.cDa

lethal (with Adf1⁰¹³⁴⁹)

Adf1^k14805

lethal (with Adf1^k14805)

Adf1⁰¹³⁴⁹

lethal (with Df(2R)BSC326)

Adf1⁰¹³⁴⁹

lethal (with Df(2R)ED1552)

Adf1^k14805

lethal | embryonic stage | recessive

Adf1^le60

lethal | recessive

partially lethal - majority die

Adf1⁰⁴⁰⁶⁵

partially lethal - majority die | recessive

Adf1^le60

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Adf1^GD1358

viable, with Scer\GAL4^Mef2.PR

Adf1^GD1358

viable, with Scer\GAL4^pnr-MD237

Adf1^GD1358

Other Phenotypes

Allele

locomotor behavior defective | adult stage, with Scer\GAL4^futsch-C380

Adf1^{S64A.S184A.UAS.Tag:FLAG}

locomotor behavior defective | larval stage, with Scer\GAL4^futsch-C380

Adf1^{S64A.S184A.UAS.Tag:FLAG}

memory defective

Adf1^hs.PD

memory defective | recessive

neuroanatomy defective

neuroanatomy defective | larval stage, with Scer\GAL4^elav-C155

Adf1^EP815

neuroanatomy defective | somatic clone, with Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

neurophysiology defective, with Scer\GAL4^futsch-C380

Adf1^{S64A.S184A.UAS.Tag:FLAG}

neurophysiology defective, with Scer\GAL4^futsch-C380, Scer\GAL80^ChAT.3.3P

Adf1^{S64A.S184A.UAS.Tag:FLAG}

neurophysiology defective | somatic clone, with Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

Adf1^DN.UAS

visible, with Scer\GAL4^Bx-MS1096

Adf1^KK111012

visible, with Scer\GAL4^ptc-559.1

Adf1^EP815

wild-type

Adf1^hs.PD

Phenotype manifest in

Allele

abdominal dorsal multidendritic neuron ddaE & dendrite

Adf1⁰¹³⁴⁹

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Adf1^EP815

bouton

Adf1^nal

bouton | ectopic

Adf1^hs.PD

dendrite

Adf1^nal

dendrite | somatic clone, with Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

dorsal multidendritic neuron ddaE & dendrite

Adf1⁰¹³⁴⁹

dorsocentral bristle | ectopic, with Scer\GAL4^ptc-559.1

Adf1^EP815

intersegmental nerve, with Scer\GAL4^elav-C155

Adf1^EP815

RP2 motor neuron

Adf1^nal

RP2 motor neuron | somatic clone, with Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

scutellar bristle | ectopic, with Scer\GAL4^ptc-559.1

Adf1^EP815

wing, with Scer\GAL4^Bx-MS1096

Adf1^KK111012

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MSANTD2

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (0)

No records found.

Rattus norvegicus (Norway rat) (0)

No records found.

Xenopus tropicalis (Western clawed frog) (1)

Xtro\LOC101734176

1 of 12

Yes

Danio rerio (Zebrafish) (7)

Drer\LOC100330838

4 of 15

Yes

Drer\si:ch211-207i20.3

4 of 15

Yes

Drer\si:zfos-128g4.1

3 of 15

Yes

Drer\zgc:152938

3 of 15

Drer\si:ch73-59f11.3

2 of 15

Yes

Drer\si:dkey-66i24.7

1 of 15

Drer\si:dkey-261j15.2

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\madf-9

5 of 15

Yes

Cele\madf-3

3 of 15

Cele\madf-4

3 of 15

Cele\madf-2

2 of 15

Yes

Cele\madf-10

2 of 15

Yes

Cele\irld-64

1 of 15

Yes

Cele\madf-5

1 of 15

Cele\nspb-10

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (10)

Atha\AT1G76880

1 of 9

Yes

Atha\AT2G38250

1 of 9

Yes

Atha\AT3G25990

1 of 9

Yes

Atha\AT5G01380

1 of 9

Yes

Atha\AT5G28300

1 of 9

Yes

Atha\AT5G47660

1 of 9

Yes

Atha\EDA31

1 of 9

Yes

Atha\GT-1

1 of 9

Yes

Atha\GT2

1 of 9

Yes

Atha\PTL

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190DFT )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Adf1

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13983

Drosophila persimilis

Dper\GL11108

Drosophila willistoni

Dwil\GK23084

Drosophila virilis

Dvir\GJ22167

Drosophila mojavensis

Dmoj\GI19291

Drosophila grimshawi

Dgri\GH21743

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150A98 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17017611

Musca domestica

House fly

Mdoa\17017603

Glossina morsitans

Tsetse fly

Gmor\GMOY000144

Glossina morsitans

Tsetse fly

Gmor\GMOY010180

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11410

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11406

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02IV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Heliconius melpomene

Postman butterfly

Hmel\HMEL014186

Rhodnius prolixus

Kissing bug

Rpro\RPRC009119

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI23773

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02G9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR012975

Strigamia maritima

European centipede

Smar\SMAR013601

Stegodyphus mimosarum

African social velvet spider

Smim\KFM69170.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_228142

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0I9A )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Adf1L

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000012255

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (29)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Hmr

1 of 10

meso18E

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Adf1

pull down, tag visualisation

(Kazemian et al., 2013)

Adf1 - CG11275

two hybrid

(Hu et al., 2017.6.13)

Adf1 - CG5292

two hybrid

(Hu et al., 2017.6.13)

Adf1 - Pc

anti bait coimmunoprecipitation, western blot

(Orsi et al., 2014)

Adf1 - Trl

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Lomaev et al., 2017)

Adf1 - cad

pull down, tag visualisation

(Cheng et al., 2013)

Adf1 - hb

pull down, tag visualisation

(Cheng et al., 2013)

Adf1 - srp

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Adf1

suppressible

Fas2

(Timmerman et al., 2013)

Adf1

suppressible

stau

(Timmerman et al., 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

CaMKII

enhanceable

Adf1

(Pla et al., 1997)

enhanceable

Adf1

(Orsi et al., 2014)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

70573

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

47082

MIST (protein-protein) - An integrated Molecular Interaction Database

Adf1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42C3-42C3

Sequence location

2R:6,662,265..6,668,085 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42C3-42C3

Limits computationally determined from genome sequence between P{lacW}geminin^k14019&P{PZ}Adf1⁰¹³⁴⁹ and P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

42C1-42C2

(determined by in situ hybridisation)

(Abdelilah-Seyfried et al., 2000)

42C1-42B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

42C1-42C4

42C3--4

(BDGP Project Members, 1994-1999)

42C2-42C7

(determined by in situ hybridisation)

(England et al., 1992)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Bloomington

28680

y¹ v¹; P{TRiP.JF03095}attP2

31500

y¹ v¹; P{TRiP.HM04004}attP2

83674

y¹ w^*; PBac{Adf1-GFP.FPTB}VK00033

11040

P{PZ}Adf1⁰¹³⁴⁹ cn¹/CyO, P{sevRas1.V12}FK1

11135

y¹ w^67c23; P{lacW}Adf1^k14805/CyO

Kyoto

123657

w¹¹¹⁸; P{RS3}Adf1^CB-5490-3

125553

w¹¹¹⁸; P{RS5}Adf1^5-HA-2159

125627

w¹¹¹⁸; P{RS5}Adf1^5-HA-2715

104084

w^*; P{GawB}Adf1^NP2090 / CyO

102168

y¹ w^67c23 ; P{lacW}Adf1^k00620 / CyO

VDRC

v102176

P{KK111012}VIE-260B

v4278

w¹¹¹⁸; P{GD1358}v4278

More stocks available...

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (39)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

DPiM_LD09689

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lomaev et al., 2017, Timmerman et al., 2013, England et al., 1992)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Adf-1 l(2)01349

(Spradling et al., 1999)

Source for merge of: Adf1 CG15845

(Bayraktaroglu, 2000.4.12)

Source for merge of: Adf1 l(2)k14805

(Kahsai et al., 2016)

Additional comments

Other Comments

Adf1 mutants have defects in larval locomotion.

(Parrish et al., 2006)

dsRNA has been made from templates generated with primers directed against this gene. RNAi of Adf1 results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.

(Parrish et al., 2006)

Adf1 may be a critical regulatory switch involved in terminal stages of synapse maturation.

(DeZazzo et al., 2000)

Comprehensive mapping of the functional domains of Adf1 to study the role of transactivators in the process of gene activation.

(Cutler et al., 1998)

The specific setting of a nucleosome and the particular histone-DNA contacts over the factor binding sites may play important roles in modulating Adf1 and Trl protein binding to the Adh distal promoter in chromatin to regulate transcription.

(Gao and Benyajati, 1998)

Shown, by partial sequencing, to be an allele of Adf1.

(Tully, 1995)

Encodes a transcription factor first identified by its binding to Adh enhancer sequences, but probably quite ubiquitous. The gene was also identified in a P-element screen for learning and memory defective mutations by Tully (FBrf0075143).

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

47082

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P05552

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KEJ0

Other crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG15845

Linkouts

BioGRID - A database of protein and genetic interactions.

70573

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG15845-RA

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

47082

MIST (protein-protein) - An integrated Molecular Interaction Database

Adf1

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

ADF1

(Blatti et al., 2015, Cheng et al., 2013)

Adf 1

(Kraut et al., 2001, Kraut et al., 2001)

Adh transcription factor 1

(Edwards et al., 2006)

CG15845

(Shukla et al., 2018, Rosa-Ferreira et al., 2015, Shukla et al., 2014, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Casola et al., 2007, Jensen et al., 2006, Kraut et al., 2001, Kraut et al., 2001)

adf-1

(Shukla et al., 2014, Dubnau et al., 2003)

adf1

(Sandstrom et al., 1998)

l(2)01349

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

l(2)04065

(BDGP Project Members, 1994-1999)

l(2)k14805

(Kahsai et al., 2016)

nal

(Guan et al., 2011, Roman and Davis, 2001, Waddell and Quinn, 2001, DeZazzo et al., 2000, DeZazzo et al., 1997, Tully, 1996, Tully, 1995)

Name Synonyms

Adh transcription factor 1

(Guan et al., 2011, Edwards et al., 2006, Jensen et al., 2006)

Nalyot

(Guan et al., 2011, Dubnau et al., 2003)

lethal (2) k14805

nalyot

(Burrell and Sahley, 2001, Kraut et al., 2001, Kraut et al., 2001, Waddell and Quinn, 2001, DeZazzo et al., 2000, DeZazzo et al., 1997)

naylot

(Roman and Davis, 2001)

Secondary FlyBase IDs

FBgn0000054
FBgn0010484
FBgn0010565
FBgn0013714
FBgn0033084
FBgn0021846

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (121)

Report Sections

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