FB2025_01 , released February 20, 2025
Gene: Dmel\Ttd14
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General Information
Symbol
Dmel\Ttd14
Species
D. melanogaster
Name
TRPL translocation defect 14
Annotation Symbol
CG30118
Feature Type
protein_coding_gene
FlyBase ID
FBgn0284257
Gene Model Status
Current
Stock Availability
11 publicly available
Gene Summary
TRPL translocation defect 14 (Ttd14) encodes a cytosolic GTP binding protein that binds to the membrane lipids 3-phosphoinositide and phosphatic acid. It has a role in recycling of internalized membrane proteins like the ion channel encoded by trpl in photoreceptor cells. [Date last reviewed: 2019-03-28] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
55C9-55C9
Sequence location
Recombination map
2-85
RefSeq locus
NT_033778 REGION:18430315..18440475
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables GTP binding
inferred from direct assay
(Cerny et al., 2015)
enables phosphatidic acid binding
inferred from direct assay
(Cerny et al., 2015)
enables phosphatidylinositol binding
inferred from direct assay
(Cerny et al., 2015)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables GTP binding
inferred from biological aspect of ancestor with PANTHER:PTN002167550
(GO Reference Genome Project, 2011-)
enables phosphatidic acid binding
inferred from biological aspect of ancestor with PANTHER:PTN002167550
(GO Reference Genome Project, 2011-)
enables phosphatidylinositol binding
inferred from biological aspect of ancestor with PANTHER:PTN002167550
(GO Reference Genome Project, 2011-)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in cellular response to light stimulus
inferred from mutant phenotype
(Cerny et al., 2015)
involved_in photoreceptor cell maintenance
inferred from mutant phenotype
(Cerny et al., 2015)
involved_in positive regulation of intracellular protein transport
inferred from mutant phenotype
(Cerny et al., 2015)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in photoreceptor cell maintenance
inferred from biological aspect of ancestor with PANTHER:PTN002167553
(GO Reference Genome Project, 2011-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Cerny et al., 2015)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
TRPL translocation defect 14 (Ttd14) encodes a cytosolic GTP binding protein that binds to the membrane lipids 3-phosphoinositide and phosphatic acid. It has a role in recycling of internalized membrane proteins like the ion channel encoded by trpl in photoreceptor cells. [Date last reviewed: 2019-03-28]
Protein Function (UniProtKB)
GTP-binding protein which is required for the light-dependent internalization of the TRPL ion channel from the rhabdomere on the apical surface of photoreceptor cells to the cell body and for the recycling of TRPL back to the rhabdomere in the dark. Binds to 3-phosphoinositide (PtdIns(3)P) and phosphatic acid and so may interact with membranes, particularly the early endosome membrane where PtdIns(3)P is predominantly localized. Plays a role in preventing photoreceptor degeneration.
(UniProt, Q7K556)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Ttd14 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7K556)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Ttd14-RA
FBtr0086724
NM_137489
2317
475
Ttd14-RB
FBtr0113360
NM_001103895
2030
471
Ttd14-RC
FBtr0113361
NM_001103896
2584
515
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Ttd14-PA
FBpp0085903
54.3
475
8.63
Q7K556
NP_611333
AAF57688
Ttd14-PB
FBpp0112272
53.5
471
9.11
NP_001097365
ABV53842
Ttd14-PC
FBpp0112273
58.2
515
8.83
A8DYI2
NP_001097366
ABV53843
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ttd14 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.94

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
anlage in statu nascendi
(Fisher et al., 2012)
anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
central brain anlage in statu nascendi
(Fisher et al., 2012)
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
hindgut anlage in statu nascendi
(Fisher et al., 2012)
posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
anterior endoderm anlage
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
amnioserosa
(Fisher et al., 2012)
amnioserosa primordium
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
Malpighian tubule primordium

Comment: reported as Malpighian tubule anlage

(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
amnioserosa
(Fisher et al., 2012)
anal pad primordium

Comment: reported as anal pad specific anlage

(Fisher et al., 2012)
anterior midgut primordium
(Fisher et al., 2012)
dorsal pharyngeal muscle primordium
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
Malpighian tubule primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
salivary gland body primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic/larval crystal cell
(Fisher et al., 2012)
dorsal pharyngeal muscle cell
(Fisher et al., 2012)
embryonic anal pad
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic Malpighian tubule
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
larval muscle system
(Fisher et al., 2012)
larval somatic muscle cell
(Fisher et al., 2012)
larval visceral muscle cell
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
• membrane
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Cerny et al., 2015)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ttd14 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ttd14
Transgenic constructs containing regulatory region of Ttd14
Aberrations (Deficiencies and Duplications) ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (0)
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (0)
Mus musculus (laboratory mouse) (0)
Xenopus tropicalis (Western clawed frog) (0)
Danio rerio (Zebrafish) (0)
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\unc-132
12 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP005005
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Ttd14. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Other Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Ttd14 - pr
    experimental knowledge based
    (Guruharsha et al., 2011)
    Summary of Genetic Interactions
    esyN Network Diagram
    Other Interaction Browsers
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-85
    Cytogenetic map
    55C9-55C9
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    55C9-55C9
    Limits computationally determined from genome sequence between P{PZ}sbb04525&P{EP}EP1081EP1081 and P{lacW}l(2)08770k04808
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    55D1-55D2
    (determined by in situ hybridisation)
    (Spradling et al., 1999)
    55C9-55C10
    55D1--2
    (BDGP Project Members, 1994-1999)
    Experimentally Determined Recombination Data
    Location

    2-85

    (FlyBase, 2016)

    2-89.9

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (11)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (73)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Cerny et al., 2015)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
          Other Comments
          Relationship to Other Genes
          Source for database merge of

          Source for merge of: CG5544 CG16859

          (Misra, 2001.8.10, FlyBase, 1996-)

          Source for merge of: l(2)08770 l(2)k04808

          (Spradling et al., 1999)

          Source for merge of: Ttd14 l(2)08770

          (Kahsai et al., 2016)
          Additional comments

          Annotations CG16859, CG5544 merged as CG30118 in release 3 of the genome annotation.

          (FlyBase Genome Annotators, 2002-2003)

          Source for merge of CG5544 CG16859 was a shared cDNA ( date:010720 ).

          (FlyBase, 1996-)
          Nomenclature History
          Source for database identify of

          Source for identity of: Ttd14 CG30118

          (Cerny et al., 2015)
          Nomenclature comments
          Etymology

          Named based on inability to internalize the ion channel transient receptor potential-like (TRPL)

          (Cerny et al., 2015)
          Synonyms and Secondary IDs (10)
          Reported As
          Symbol Synonym
          CG16859
          (Misra, 2001.8.10)
          CG30118
          (Lee et al., 2023, National Institute of Genetics Fly Stocks, 2023-, Everman et al., 2019, Ashwal-Fluss et al., 2014, Luck et al., 2014, Huang et al., 2013, Buffin and Gho, 2010, Kong et al., 2010, Keleman et al., 2009.8.5)
          CG5544
          TTD14
          (Schopf and Huber, 2017)
          Ttd14
          l(2)08770
          l(2)k04808
          ttd14
          (Cerny et al., 2015)
          Name Synonyms
          TRPL translocation defect 14
          lethal (2) 08770
          Secondary FlyBase IDs
          • FBgn0050118
          • FBgn0034347
          • FBgn0034348
          • FBgn0022199
          • FBgn0010652
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 38 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          MIST (protein-protein) - An integrated Molecular Interaction Database
          References (38)