FB2025_05 , released December 11, 2025
Gene: Dmel\Mcm3
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General Information
Symbol
Dmel\Mcm3
Species
D. melanogaster
Name
Minichromosome maintenance 3
Annotation Symbol
CG4206
Feature Type
protein_coding_gene
FlyBase ID
FBgn0284442
Gene Model Status
Current
Stock Availability
7 publicly available
Enzyme Name (EC)
DNA 3'-5' helicase (5.6.2.4)
Gene Summary
Minichromosome maintenance 3 (Mcm3) encodes a component of the MCM2-7 hexamer, which forms part of the CMG complex, together with the product of CDC45L and GINS proteins. The CMG complex is the main DNA helicase that functions during DNA replication. [Date last reviewed: 2019-03-28] (FlyBase Gene Snapshot)
All Summaries
Also Known As

small mushroom bodies, smu, DmMCM3, PCR2

Key Links
Genomic Location
Cytogenetic map
4F5-4F5
Sequence location
Recombination map
1-11
RefSeq locus
NC_004354 REGION:5323855..5326738
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (15 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
contributes_to 3'-5' DNA helicase activity
inferred from direct assay
(Moyer et al., 2006)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR031327
(InterPro Project Members, 2004-)
(InterPro Project Members, 2004-)
enables DNA binding
inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR031327
(InterPro Project Members, 2004-)
(InterPro Project Members, 2004-)
enables single-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
contributes_to single-stranded DNA helicase activity
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
Biological Process (7 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in mushroom body development
inferred from mutant phenotype
(Blumröder et al., 2016, de Belle and Heisenberg, 1996)
involved_in neuroblast proliferation
inferred from mutant phenotype
(Blumröder et al., 2016)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in DNA replication initiation
inferred from electronic annotation with InterPro:IPR008046
(InterPro Project Members, 2004-)
involved_in DNA replication
inferred from electronic annotation with InterPro:IPR018525
(InterPro Project Members, 2004-)
involved_in DNA strand elongation involved in DNA replication
inferred from biological aspect of ancestor with PANTHER:PTN000179713
(GO Reference Genome Project, 2011-)
involved_in double-strand break repair via break-induced replication
inferred from biological aspect of ancestor with PANTHER:PTN000179713
(GO Reference Genome Project, 2011-)
involved_in mitotic DNA replication initiation
inferred from biological aspect of ancestor with PANTHER:PTN000179713
(GO Reference Genome Project, 2011-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of MCM complex
inferred from electronic annotation with InterPro:IPR008046
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000179712
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MCM family. (Q9XYU1)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
3'-5' DNA helicase activity
Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction (5.6.2.4)
Summaries
Gene Snapshot
Minichromosome maintenance 3 (Mcm3) encodes a component of the MCM2-7 hexamer, which forms part of the CMG complex, together with the product of CDC45L and GINS proteins. The CMG complex is the main DNA helicase that functions during DNA replication. [Date last reviewed: 2019-03-28]
Gene Group (FlyBase)
MCM2-7 COMPLEX -
The heterohexameric MCM2-7 complex is the helicase motor of the CMG (Cdc45-MCM-GINS) replicative helicase of eukaryotes. The six MCM subunits are related to one another and are members of the AAA+ (ATPases associated with diverse cellular activities) superfamily. Hydrolysis of ATP by the individual MCM subunits catalyses the conformational changes that drive unwinding of the DNA duplex. However, the eukaryotic MCM complex alone has no or little helicase activity, requiring the other components of the CMG complex (Cdc45 and the GINS complex) for activation. (Adapted from PMID:23412083.)
CMG COMPLEX -
The CMG (Cdc45-MCM-GINS) complex is the eukaryotic replicative helicase, the enzyme that unwinds double-stranded DNA at replication forks. It is a macromolecular assembly of three replication factors: the Cdc45 protein, and the MCM and GINS complexes. All three components are essential for CMG function, with the MCM2-7 complex functioning as the molecular motor that harnesses the energy of ATP hydrolysis to catalyse strand separation. (Adapted from PMID:23412083.)
MCM FAMILY HELICASES -
The MCM (minichromosome maintenance) family comprise DNA helicases sharing a common 'MCM domain'. MCM2-7 form a heterohexameric complex that is essential for initiation of DNA replication and also acts during elongation as the replicative helicase. MCM8 and MCM9 are not part of this complex - they have roles in replication elongation, meiotic recombination and DNA repair. MCM8 and 9 arose early during eukaryotic evolution, though one or both have been lost in multiple lineages: in mammals, MCM8 and MCM9 form a functional heterodimeric complex; D. melanogaster and related species possess MCM8 only; while other species (e.g. yeast, C. elegans) have neither. (Adapted from PMID:23412083, PMID:30743181 and FBrf0188222.)
Protein Function (UniProtKB)
Acts as a component of the Mcm2-7 complex (Mcm complex) (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
(UniProt, Q9XYU1)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Mcm3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9XYU1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mcm3-RA
FBtr0070762
NM_078493
2747
819
Mcm3-RB
FBtr0340476
NM_001297955
2744
818
Additional Transcript Data and Comments
Reported size (kB)

3.0 (northern blot)

(Ohno et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Mcm3-PA
FBpp0070729
90.9
819
6.35
Q9XYU1
NP_511048
AAF46023
Mcm3-PB
FBpp0309417
90.8
818
6.26
X2JAI4
NP_001284884
AHN59355
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

819 (aa); 90.0 (kD predicted)

(Feger, 1999)

819 (aa)

(Ohno et al., 1998)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).

(UniProt, Q9XYU1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm3 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.23

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | faint | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
head mesoderm
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
presumptive embryonic/larval central nervous system
(Feger, 1999)
central brain primordium
(Fisher et al., 2012)
embryonic ganglion mother cell
(Fisher et al., 2012)
embryonic neuroblast of ventral nerve cord primordium
(Fisher et al., 2012)
larval lateral VNC glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
procephalic neuroblast
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
larval lateral VNC surface glial cell
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 9

Comment: reference states 0-4 hr AEL

(Ohno et al., 1998)
adult stage | female
(Ohno et al., 1998)
unfertilized egg stage
unfertilized egg
(Ohno et al., 1998)
Additional Descriptive Data

Mcm3 transcript expression pattern resembles that of DNA replication. Mcm3 transcript is detected at high levels in the central nervous system and at very low levels in the gut of stage 11-12 embryos.

(Feger, 1999)

Mcm3 transcript levels are high in unfertilized eggs, slightly higher in 2-4 hour embryos, and are lower after 4 hours of embryogenesis. Levels are low in other developmental stages, with the exception of adult females.

(Ohno et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm3 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mcm3
Transgenic constructs containing regulatory region of Mcm3
Aberrations (Deficiencies and Duplications) ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (9)
Hsap\MCM3
13 of 14
Yes
Yes
Hsap\MCM2
3 of 14
No
No
Hsap\MCM4
3 of 14
No
No
Hsap\MCM5
3 of 14
No
No
1  
Hsap\MCM6
3 of 14
No
No
Hsap\MCM7
3 of 14
No
No
Hsap\MCM8
3 of 14
No
No
Hsap\MCM9
3 of 14
No
Yes
Hsap\MCMDC2
2 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (9)
Rnor\Mcm3
14 of 14
Yes
Yes
Rnor\Mcm2
3 of 14
No
No
Rnor\Mcm4
3 of 14
No
No
Rnor\Mcm5
3 of 14
No
No
Rnor\Mcm7
3 of 14
No
No
Rnor\Mcm8
3 of 14
No
No
Rnor\Mcm6
2 of 14
No
No
Rnor\Mcm9
2 of 14
No
Yes
Rnor\Mcmdc2
2 of 14
No
Yes
Mus musculus (laboratory mouse) (9)
Mmus\Mcm3
13 of 14
Yes
Yes
Mmus\Mcm2
3 of 14
No
No
Mmus\Mcm4
3 of 14
No
No
Mmus\Mcm5
3 of 14
No
No
Mmus\Mcm6
3 of 14
No
No
Mmus\Mcm7
3 of 14
No
No
Mmus\Mcm8
3 of 14
No
No
Mmus\Mcm9
2 of 14
No
Yes
Mmus\Mcmdc2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (12)
Xtro\zmcm3
11 of 13
Yes
Yes
Xtro\mcm2
2 of 13
No
No
Xtro\mcm4
2 of 13
No
No
Xtro\mcm5
2 of 13
No
No
Xtro\mcm6.2
2 of 13
No
No
Xtro\mcm6
2 of 13
No
No
Xtro\mcm7
2 of 13
No
No
Xtro\mcm8
2 of 13
No
No
Xtro\mcm9
2 of 13
No
Yes
Xtro\hdlbp
1 of 13
No
No
Xtro\mcm3
1 of 13
No
Yes
Xtro\otub2
1 of 13
No
No
Danio rerio (Zebrafish) (11)
Drer\mcm3
14 of 14
Yes
Yes
Drer\mcm3l
13 of 14
No
Yes
Drer\mcm4
3 of 14
No
No
Drer\mcm5
3 of 14
No
No
Drer\mcm6
3 of 14
No
No
Drer\mcm6l
3 of 14
No
No
Drer\mcm8
3 of 14
No
No
Drer\mcm9
3 of 14
No
Yes
Drer\mcm2
2 of 14
No
No
Drer\mcm7
2 of 14
No
No
Drer\mcmdc2
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\mcm-3
13 of 14
Yes
Yes
Cele\mcm-4
3 of 14
No
No
Cele\mcm-5
3 of 14
No
No
Cele\mcm-7
3 of 14
No
No
Cele\mcm-2
2 of 14
No
No
Cele\mcm-6
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (10)
Agam\AgaP_AGAP001135
12 of 12
Yes
Yes
Agam\AgaP_AGAP001901
3 of 12
No
Yes
Agam\AgaP_AGAP002580
3 of 12
No
No
Agam\AgaP_AGAP002581
3 of 12
No
No
Agam\AgaP_AGAP004275
3 of 12
No
No
Agam\AgaP_AGAP004408
3 of 12
No
No
Agam\AgaP_AGAP004956
3 of 12
No
No
Agam\AgaP_AGAP005800
3 of 12
No
No
Agam\MCM6_ANOGA
3 of 12
No
No
Agam\AgaP_AGAP012691
2 of 12
No
No
Arabidopsis thaliana (thale-cress) (8)
Atha\MCM3
13 of 13
Yes
Yes
Atha\MCM2
3 of 13
No
No
Atha\MCM4
3 of 13
No
No
Atha\MCM5
3 of 13
No
No
Atha\MCM6
3 of 13
No
No
Atha\MCM8
3 of 13
No
No
Atha\MCM9
3 of 13
No
Yes
Atha\PRL
3 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\MCM3
13 of 13
Yes
Yes
Scer\MCM2
2 of 13
No
No
Scer\MCM4
2 of 13
No
No
Scer\MCM5
2 of 13
No
No
Scer\MCM6
2 of 13
No
No
Scer\MCM7
2 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\mcm3
12 of 12
Yes
Yes
Spom\mcm2
2 of 12
No
No
Spom\mcm4
2 of 12
No
No
Spom\mcm5
2 of 12
No
No
Spom\mcm6
2 of 12
No
No
Spom\mcm7
2 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\yifB
1 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Mcm3. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (7)
dpa
6 of 13
Mcm2
6 of 13
Mcm5
6 of 13
Mcm6
6 of 13
Mcm7
6 of 13
rec
6 of 13
mei-218
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MCM3; minichromosome maintenance complex component 3
    13 of 14
    602693
        MCM2; minichromosome maintenance complex component 2
        3 of 14
        116945
        MCM4; minichromosome maintenance complex component 4
        3 of 14
        602638
        MCM5; minichromosome maintenance complex component 5
        3 of 14
        602696
         
        MCM6; minichromosome maintenance complex component 6
        3 of 14
        601806
        MCM7; minichromosome maintenance complex component 7
        3 of 14
        600592
            MCM8; minichromosome maintenance 8 homologous recombination repair factor
            3 of 14
            608187
            MCM9; minichromosome maintenance 9 homologous recombination repair factor
            3 of 14
            610098
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            Mcm3 - CG3430
            experimental knowledge based
            (Guruharsha et al., 2011)
            Mcm3 - Cdc45
            anti tag coimmunoprecipitation, molecular weight estimation by silver staining, anti tag western blot, anti bait coimmunoprecipitation, western blot, mass spectrometry studies of complexes, cosedimentation, electron microscopy, pull down
            (Costa et al., 2014, Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006, Loebel et al., 2000)
            Mcm3 - Mcm2
            anti tag coimmunoprecipitation, western blot, cosedimentation, electron microscopy, pull down
            (Costa et al., 2014, Costa et al., 2011)
            Mcm3 - Mcm5
            electron microscopy, cosedimentation, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, experimental knowledge based, pull down
            (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011, Loebel et al., 2000)
            Mcm3 - Mcm6
            cosedimentation, electron microscopy
            (Costa et al., 2011)
            Mcm3 - Mcm7
            cosedimentation, electron microscopy, experimental knowledge based
            (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)
            Mcm3 - Psf1
            pull down, western blot, anti tag coimmunoprecipitation, cosedimentation, electron microscopy
            (Costa et al., 2014, Costa et al., 2011)
            Mcm3 - Psf2
            anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, pull down, cosedimentation, electron microscopy
            (Costa et al., 2014, Costa et al., 2011, Moyer et al., 2006)
            Mcm3 - Psf3
            cosedimentation, electron microscopy
            (Costa et al., 2011)
            Mcm3 - Sld5
            cosedimentation, electron microscopy, pull down, western blot, anti tag coimmunoprecipitation
            (Costa et al., 2014, Costa et al., 2011)
            Mcm3 - dpa
            anti tag coimmunoprecipitation, western blot, cosedimentation, electron microscopy
            (Costa et al., 2014, Costa et al., 2011)
            Mcm3 - lok
            enzymatic study, autoradiography, molecular weight estimation by silver staining
            (Ilves et al., 2012)
            Summary of Genetic Interactions
            Interaction Browsers
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            External Data
            Subunit Structure (UniProtKB)
            Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).
            (UniProt, Q9XYU1 )
            Linkouts
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            X
            Recombination map
            1-11
            Cytogenetic map
            4F5-4F5
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            4F5-4F5
            Limits computationally determined from genome sequence between P{EP}EP425 and P{EP}CG3249EP1400
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Mcm3 maps to 4F9-10/5A1-2.
            (Feger, 1999)
            4F9-5A2
            (Feger, 1999.2.1)
            4F9-5A2
            (determined by in situ hybridisation)
            (Feger, 1999)
            4F-4F
            (determined by in situ hybridisation)
            (Ohno et al., 1998)
            4F12-4F12
            (de Belle and Heisenberg, 1996)
            Experimentally Determined Recombination Data
            Location

            1-11

            (FlyBase, 2016, de Belle and Heisenberg, 1996)

            1-13.4

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (7)
            Genomic Clones (13)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (33)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              Antibody Information
              Laboratory Generated Antibodies
               
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                  Other Comments

                  The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

                  (Moyer et al., 2006)
                  Relationship to Other Genes
                  Source for database merge of

                  Source for merge of: Mcm3 Mcp-PCR2

                  (Ohno et al., 1998)

                  Source for merge of: Mcm3 smu

                  (Blumröder et al., 2016)
                  Additional comments
                  Nomenclature History
                  Source for database identify of

                  Source for identity of: Mcm3 CG4206

                  (Zhong, 1999.11)
                  Nomenclature comments
                  Etymology
                  Synonyms and Secondary IDs (16)
                  Reported As
                  Symbol Synonym
                  CG4206
                  (Çiçek et al., 2016, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)
                  DmMCM3
                  (Feger, 1999, Ohno, 1998.1.5, Ohno et al., 1998)
                  DmMcm3
                  (Loebel et al., 2000)
                  DmeMCM3
                  (Blanton et al., 2005)
                  MCM3
                  (Bhargava et al., 2020, Hua and Orr-Weaver, 2017, Bleichert et al., 2015, Crevel et al., 2011, Gallone et al., 2011, Ilves et al., 2010, Li et al., 2010, Crevel et al., 2007, Hong et al., 2007, Hediger and Gasser, 2006, Frolov et al., 2005, Claycomb et al., 2002, Stevaux et al., 2002, Crevel et al., 2001, Frolov et al., 2001)
                  McM3
                  (De Lucia et al., 2005)
                  Mcm3
                  (Curnutte et al., 2023, Rubio-Ferrera et al., 2022, Yassin et al., 2022, Kose et al., 2020, Burnham et al., 2019, Parker et al., 2019, Vorobyeva and Mazina, 2019, Kohzaki, 2018, Kong et al., 2016, Völker-Albert et al., 2016, Chen et al., 2014, Costa et al., 2014, Liang et al., 2014, Yan et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Longworth et al., 2012, Costa et al., 2011, Kondo and Perrimon, 2011, Neumüller et al., 2011, Maqbool et al., 2010, Somma et al., 2008, Spresser et al., 2008, Akashi et al., 2006, Foglietti et al., 2006, Ko et al., 2006, Moyer et al., 2006, De Lucia et al., 2005, Loop et al., 2004, Cayirlioglu et al., 2003)
                  Mcp-PCR2
                  PCR2
                  (Ramirez Moreno et al., 2021, Su et al., 1997)
                  dMCM3
                  (Duronio et al., 1998)
                  dMcm3
                  (Sahashi et al., 2013)
                  mcm3
                  (Zhang et al., 2018, Blumröder et al., 2016)
                  smu
                  (Blumröder et al., 2016, Serway et al., 2009, Helfrich-Forster et al., 2002)
                  Name Synonyms
                  Minichromosome maintenance 3
                  (Somma et al., 2008, Duronio et al., 1998)
                  Minichromosome maintenance-PCR2
                  small mushroom bodies
                  (Serway et al., 2009, Dunkelberger et al., 2008, Dunkelberger et al., 2007, Helfrich-Forster et al., 2002, de Belle and Heisenberg, 1996)
                  Secondary FlyBase IDs
                  • FBgn0024332
                  • FBgn0020632
                  • FBgn0019654
                  Datasets (1)
                  Study focus (1)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  • bait_protein
                  modENCODE_replication_factor_ChIP
                  Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.
                  Study result (0)
                  Result
                  Result Type
                  Title
                  External Crossreferences and Linkouts ( 50 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                  UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                  UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                  Other crossreferences
                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  InterPro - A database of protein families, domains and functional sites
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  PDB - An information portal to biological macromolecular structures
                  Linkouts
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                  iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                  KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                  Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                  References (111)