FB2021_01, released Feb 18, 2021

Gene: Dmel\Mcm3

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General Information
Symbol
Dmel\Mcm3
Species
D. melanogaster
Name
Minichromosome maintenance 3
Annotation Symbol
CG4206
Feature Type
protein_coding_gene
FlyBase ID
FBgn0284442
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Snapshot
Minichromosome maintenance 3 (Mcm3) encodes a component of the MCM2-7 hexamer, which forms part of the CMG complex, together with the product of CDC45L and GINS proteins. The CMG complex is the main DNA helicase that functions during DNA replication. [Date last reviewed: 2019-03-28]
Other Summaries
Also Known As

small mushroom bodies, smu, DmMCM3

Key Links
Genomic Location
Cytogenetic map
4F5-4F12
Sequence location
X:5,323,855..5,326,738 [+]
Recombination map

1-11

RefSeq locus
NC_004354 REGION:5323855..5326738
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
contributes_to 3'-5' DNA helicase activity
inferred from direct assay
(Moyer et al., 2006)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008046, InterPro:IPR018525
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables DNA replication origin binding
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(assigned by GO_Central )
(Gaudet et al., 2011)
enables single-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in mushroom body development
inferred from mutant phenotype
(Blumröder et al., 2016, de Belle and Heisenberg, 1996)
involved_in neuroblast proliferation
inferred from mutant phenotype
(Blumröder et al., 2016)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in DNA strand elongation involved in DNA replication
inferred from biological aspect of ancestor with PANTHER:PTN000179713
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in double-strand break repair via break-induced replication
inferred from biological aspect of ancestor with PANTHER:PTN000179713
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in mitotic DNA replication initiation
inferred from biological aspect of ancestor with PANTHER:PTN000179713
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication
inferred from biological aspect of ancestor with PANTHER:PTN000179713
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of MCM complex
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MCM family. (Q9XYU1)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
MCM2-7 COMPLEX -
The heterohexameric MCM2-7 complex is the helicase motor of the CMG (Cdc45-MCM-GINS) replicative helicase of eukaryotes. The six MCM subunits are related to one another and are members of the AAA+ (ATPases associated with diverse cellular activities) superfamily. Hydrolysis of ATP by the individual MCM subunits catalyses the conformational changes that drive unwinding of the DNA duplex. However, the eukaryotic MCM complex alone has no or little helicase activity, requiring the other components of the CMG complex (Cdc45 and the GINS complex) for activation. (Adapted from PMID:23412083.)
CMG COMPLEX -
The CMG (Cdc45-MCM-GINS) complex is the eukaryotic replicative helicase, the enzyme that unwinds double-stranded DNA at replication forks. It is a macromolecular assembly of three replication factors: the Cdc45 protein, and the MCM and GINS complexes. All three components are essential for CMG function, with the MCM2-7 complex functioning as the molecular motor that harnesses the energy of ATP hydrolysis to catalyse strand separation. (Adapted from PMID:23412083.)
Protein Function (UniProtKB)
Acts as component of the Mcm2-7 complex (Mcm complex) (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
(UniProt, Q9XYU1)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Mcm3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mcm3-RA
FBtr0070762
NM_078493
2747
819
Mcm3-RB
FBtr0340476
NM_001297955
2744
818
Additional Transcript Data and Comments
Reported size (kB)

3.0 (northern blot)

(Ohno et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Mcm3-PA
FBpp0070729
90.9
819
6.35
NP_511048
AAF46023
Mcm3-PB
FBpp0309417
90.8
818
6.26
NP_001284884
AHN59355
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

819 (aa); 90.0 (kD predicted)

(Feger, 1999)

819 (aa)

(Ohno et al., 1998)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).

(UniProt, Q9XYU1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm3 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 12
presumptive embryonic/larval central nervous system
(Feger, 1999)
central brain primordium
(Fisher et al., 2012)
embryonic ganglion mother cell
(Fisher et al., 2012)
embryonic neuroblast of ventral nerve cord primordium
(Fisher et al., 2012)
lateral cord glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
procephalic neuroblast
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | faint | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
head mesoderm
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
lateral cord surface glial cell
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
(Ohno et al., 1998)
unfertilized egg stage
unfertilized egg
(Ohno et al., 1998)
embryonic stage 1 -- 9

Comment: reference states 0-4 hr AEL

(Ohno et al., 1998)
Additional Descriptive Data

Mcm3 transcript expression pattern resembles that of DNA replication. Mcm3 transcript is detected at high levels in the central nervous system and at very low levels in the gut of stage 11-12 embryos.

(Feger, 1999)

Mcm3 transcript levels are high in unfertilized eggs, slightly higher in 2-4 hour embryos, and are lower after 4 hours of embryogenesis. Levels are low in other developmental stages, with the exception of adult females.

(Ohno et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm3 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mcm3
Transgenic constructs containing regulatory region of Mcm3
Aberrations (Deficiencies and Duplications) ( 4 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\MCM3
14 of 15
Yes
Yes
Hsap\MCM2
2 of 15
No
No
Hsap\MCM4
2 of 15
No
No
Hsap\MCM5
2 of 15
No
No
Hsap\MCM6
2 of 15
No
No
Hsap\MCM7
2 of 15
No
No
Hsap\MCM8
2 of 15
No
No
Hsap\MCM9
2 of 15
No
Yes
Hsap\MCMDC2
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Mcm3
13 of 15
Yes
Yes
Mmus\Mcm2
2 of 15
No
No
Mmus\Mcm4
2 of 15
No
No
Mmus\Mcm5
2 of 15
No
No
Mmus\Mcm6
2 of 15
No
No
Mmus\Mcm7
2 of 15
No
No
Mmus\Mcm8
2 of 15
No
No
Mmus\Mcm9
2 of 15
No
Yes
Rattus norvegicus (Norway rat) (10)
Rnor\Mcm3
13 of 13
Yes
Yes
Rnor\Mcm2
2 of 13
No
No
Rnor\Mcm4
2 of 13
No
No
Rnor\Mcm5
2 of 13
No
No
Rnor\Mcm6
2 of 13
No
No
Rnor\Mcm7
2 of 13
No
No
Rnor\Mcm8
2 of 13
No
No
Rnor\Mcm9
2 of 13
No
Yes
Rnor\Asf1a
1 of 13
No
No
Rnor\Mcmdc2
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (10)
Xtro\mcm3
13 of 12
Yes
Yes
Xtro\mcm3
12 of 12
No
Yes
Xtro\mcm2
2 of 12
No
No
Xtro\mcm4
2 of 12
No
No
Xtro\mcm5
2 of 12
No
No
Xtro\mcm6.2
2 of 12
No
No
Xtro\mcm6
2 of 12
No
No
Xtro\mcm7
2 of 12
No
No
Xtro\mcm8
2 of 12
No
No
Xtro\mcm9
2 of 12
No
Yes
Danio rerio (Zebrafish) (11)
Drer\mcm3
14 of 15
Yes
Yes
Drer\mcm3l
13 of 15
No
Yes
Drer\mcm2
2 of 15
No
No
Drer\mcm4
2 of 15
No
No
Drer\mcm5
2 of 15
No
No
Drer\mcm6
2 of 15
No
No
Drer\mcm6l
2 of 15
No
No
Drer\mcm7
2 of 15
No
No
Drer\mcm8
2 of 15
No
No
Drer\mcm9
2 of 15
No
Yes
Drer\mcmdc2
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\mcm-3
12 of 15
Yes
Yes
Cele\mcm-2
2 of 15
No
No
Cele\mcm-6
2 of 15
No
No
Cele\mcm-7
2 of 15
No
No
Cele\mcm-4
1 of 15
No
No
Cele\mcm-5
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (8)
Atha\MCM3
9 of 9
Yes
Yes
Atha\MCM2
1 of 9
No
No
Atha\MCM4
1 of 9
No
No
Atha\MCM5
1 of 9
No
No
Atha\MCM6
1 of 9
No
No
Atha\MCM8
1 of 9
No
No
Atha\MCM9
1 of 9
No
Yes
Atha\PRL
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\MCM3
12 of 15
Yes
Yes
Scer\MCM2
1 of 15
No
No
Scer\MCM4
1 of 15
No
No
Scer\MCM5
1 of 15
No
No
Scer\MCM6
1 of 15
No
No
Scer\MCM7
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\mcm3
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902KP )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00006952
Drosophila simulans
Dsim\Mcm3
Drosophila sechellia
Dsec\GM12401
Drosophila erecta
Dere\Mcm3
Drosophila yakuba
Dyak\Mcm3
Drosophila ananassae
Dana\GF21337
Drosophila pseudoobscura pseudoobscura
Dpse\GA18030
Drosophila persimilis
Dper\GL14679
Drosophila willistoni
Dwil\GK18502
Drosophila virilis
Dvir\GJ14777
Drosophila mojavensis
Dmoj\GI11075
Drosophila grimshawi
Dgri\GH24613
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501HZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17019724
Glossina morsitans
Tsetse fly
Gmor\GMOY002467
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_01960
Mayetiola destructor
Hessian fly
Mdes\Mdes013985
Mayetiola destructor
Hessian fly
Mdes\Mdes000213
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011811
Anopheles darlingi
American malaria mosquito
Adar\ADAC009254
Anopheles gambiae
Malaria mosquito
Agam\AGAP001135
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ012309
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W026I )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA013590
Danaus plexippus
Monarch butterfly
Dple\DPOGS204101-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL012924
Apis florea
Little honeybee
Aflo\2348939076
Apis mellifera
Western honey bee
Amel\Mcm3
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP19104
Bombus terrestris
Buff-tailed bumblebee
Bter\15213166
Linepithema humile
Argentine ant
Lhum\LH16331
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001b32
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG010679
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_05249
Tribolium castaneum
Red flour beetle
Tcas\TC002088
Pediculus humanus
Human body louse
Phum\PHUM496000
Rhodnius prolixus
Kissing bug
Rpro\RPRC009945
Cimex lectularius
Bed bug
Clec\CLEC007232
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005644
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_01192
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01I1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR014655
Ixodes scapularis
Black-legged tick
Isca\ISCW023354
Stegodyphus mimosarum
African social velvet spider
Smim\KFM64693.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur07g00350
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_305021
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02B0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mcm3_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mcm3
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000001519
Gallus gallus
Domestic chicken
Ggal\MCM3
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (7)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
dpa
4 of 10
Mcm2
4 of 10
Mcm5
4 of 10
Mcm6
4 of 10
Mcm7
4 of 10
rec
4 of 10
mei-218
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MCM3; minichromosome maintenance complex component 3
    14 of 15
    602693
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Mcm3 - CG3430
        experimental knowledge based
        (Guruharsha et al., 2011)
        Mcm3 - Cdc45
        anti bait coimmunoprecipitation, western blot, mass spectrometry studies of complexes, anti tag coimmunoprecipitation, molecular weight estimation by silver staining, anti tag western blot, pull down, cosedimentation, electron microscopy
        (Costa et al., 2014, Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006, Loebel et al., 2000)
        Mcm3 - Mcm2
        anti tag coimmunoprecipitation, western blot, pull down, cosedimentation, electron microscopy
        (Costa et al., 2014, Costa et al., 2011)
        Mcm3 - Mcm5
        anti tag coimmunoprecipitation, western blot, electron microscopy, cosedimentation, anti bait coimmunoprecipitation, pull down, experimental knowledge based
        (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011, Loebel et al., 2000)
        Mcm3 - Mcm6
        cosedimentation, electron microscopy
        (Costa et al., 2011)
        Mcm3 - Mcm7
        electron microscopy, cosedimentation, experimental knowledge based
        (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)
        Mcm3 - Psf1
        anti tag coimmunoprecipitation, western blot, cosedimentation, electron microscopy, pull down
        (Costa et al., 2014, Costa et al., 2011)
        Mcm3 - Psf2
        cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation
        (Costa et al., 2014, Costa et al., 2011, Moyer et al., 2006)
        Mcm3 - Psf3
        cosedimentation, electron microscopy
        (Costa et al., 2011)
        Mcm3 - Sld5
        anti tag coimmunoprecipitation, western blot, cosedimentation, electron microscopy, pull down
        (Costa et al., 2014, Costa et al., 2011)
        Mcm3 - dpa
        anti tag coimmunoprecipitation, western blot, cosedimentation, electron microscopy
        (Costa et al., 2014, Costa et al., 2011)
        Mcm3 - lok
        enzymatic study, autoradiography, molecular weight estimation by silver staining
        (Ilves et al., 2012)
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).
        (UniProt, Q9XYU1 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map

        1-11

        Cytogenetic map
        4F5-4F12
        Sequence location
        X:5,323,855..5,326,738 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        4F5-4F12
        Limits computationally determined from genome sequence between P{EP}EP425 and P{EP}CG3249EP1400; Left limit from (method unavailable) (FBrf0090884) Right limit from (method unavailable) (FBrf0090884)
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        4F9-5A2
        (Feger, 1999.2.1)
        Mcm3 maps to 4F9-10/5A1-2.
        (Feger, 1999)
        4F9-5A2
        (determined by in situ hybridisation)
        (Feger, 1999)
        4F-4F
        (determined by in situ hybridisation)
        (Ohno et al., 1998)
        4F12-4F12
        (de Belle and Heisenberg, 1996)
        Experimentally Determined Recombination Data
        Location

        1-11

        (FlyBase, 2016, de Belle and Heisenberg, 1996)

        1-13.4

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (4)
        Genomic Clones (13)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (33)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: Mcm3 CG4206

          (Zhong, 1999.11)
          Source for database merge of

          Source for merge of: Mcm3 Mcp-PCR2

          (Ohno et al., 1998)

          Source for merge of: Mcm3 smu

          (Blumröder et al., 2016)
          Additional comments
          Other Comments

          The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

          (Moyer et al., 2006)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 38 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (16)
          Reported As
          Symbol Synonym
          CG4206
          (Çiçek et al., 2016, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)
          DmMCM3
          (Feger, 1999, Ohno, 1998.1.5, Ohno et al., 1998)
          DmMcm3
          (Loebel et al., 2000)
          DmeMCM3
          (Blanton et al., 2005)
          MCM3
          (Bhargava et al., 2020, Hua and Orr-Weaver, 2017, Bleichert et al., 2015, Crevel et al., 2011, Gallone et al., 2011, Ilves et al., 2010, Li et al., 2010, Crevel et al., 2007, Hong et al., 2007, Hediger and Gasser, 2006, Frolov et al., 2005, Claycomb et al., 2002, Stevaux et al., 2002, Crevel et al., 2001, Frolov et al., 2001)
          McM3
          (De Lucia et al., 2005)
          Mcm3
          (Kose et al., 2020, Burnham et al., 2019, Parker et al., 2019, Vorobyeva and Mazina, 2019, Kohzaki, 2018, Kong et al., 2016, Völker-Albert et al., 2016, Chen et al., 2014, Costa et al., 2014, Liang et al., 2014, Yan et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Longworth et al., 2012, Costa et al., 2011, Kondo and Perrimon, 2011, Neumüller et al., 2011, Maqbool et al., 2010, Somma et al., 2008, Spresser et al., 2008, Akashi et al., 2006, Foglietti et al., 2006, Ko et al., 2006, Moyer et al., 2006, De Lucia et al., 2005, Loop et al., 2004, Cayirlioglu et al., 2003)
          Mcp-PCR2
          PCR2
          (Su et al., 1997)
          dMCM3
          (Duronio et al., 1998)
          dMcm3
          (Sahashi et al., 2013)
          mcm3
          (Zhang et al., 2018, Blumröder et al., 2016)
          smu
          (Blumröder et al., 2016, Serway et al., 2009, Helfrich-Forster et al., 2002)
          Name Synonyms
          Minichromosome maintenance 3
          (Somma et al., 2008, Duronio et al., 1998)
          Minichromosome maintenance-PCR2
          small mushroom bodies
          (Serway et al., 2009, Dunkelberger et al., 2008, Dunkelberger et al., 2007, Helfrich-Forster et al., 2002, de Belle and Heisenberg, 1996)
          Secondary FlyBase IDs
          • FBgn0024332
          • FBgn0020632
          • FBgn0019654
          Datasets (1)
          Study focus (1)
          Experimental Role
          Project
          Project Type
          Title
          • bait_protein
          modENCODE_replication_factor_ChIP
          Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.
          References (101)