FlyBase Gene Report: Dmel\Imp

(Fisher et al., 2012)

embryonic stage 4 -- 14

(Nielsen et al., 2000)

embryonic stage 5

procephalic neurogenic region

(Lecuyer et al., 2007)

embryonic stage 6 -- 7

organism | striped

Comment: 4 diffuse circumferential stripes that broaded with time

(Nielsen et al., 2000)

embryonic stage 8 -- 9

ventral furrow

(Nielsen et al., 2000)

cephalic furrow

(Nielsen et al., 2000)

ventral neurectoderm

(Nielsen et al., 2000)

(Nielsen et al., 2000)

embryonic stage 9 -- 17

organism

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)

embryonic stage 10 -- 12

ventral nerve cord primordium

(Nielsen et al., 2000)

embryonic stage 10 -- 16

presumptive embryonic/larval central nervous system

(Nielsen et al., 2000)

embryonic stage 11

embryonic epidermis

(Nielsen et al., 2000)

embryonic stage 12 -- 15

larval nervous system

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

Additional Descriptive Data

Imp transcripts are detected early and late in embryogenesis but transcripts containing the 3' UTR extension (~8.5 kb) are only detected at later stages and appear after the maternal-to-zygotic transition. The 3' UTR extension isoforms are highly enriched in nervous system tissues.

(Hilgers et al., 2011)

Imp expression at embryonic stages 1-4 is localized to the posterior pole of the embryo.

(Lecuyer et al., 2007)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

organism

Comment: in cytoplasm

(Adolph et al., 2009)

larval ventral nerve cord

(Adolph et al., 2009)

embryonic stage 14 -- 16

(Adolph et al., 2009)

embryonic stage 16

sensory neuron

(Adolph et al., 2009)

scolopale cell

(Adolph et al., 2009)

thecogen cell

(Adolph et al., 2009)

first instar larval stage

larval neuroblast | subset

(Yang et al., 2017)

early third instar larval stage

larval neuroblast | subset

(Yang et al., 2017)

wandering third instar larval stage

neuroblast MBp

(Yang et al., 2017)

oogenesis

oocyte

(Geng and Macdonald, 2006)

nurse cell

(Geng and Macdonald, 2006)

follicle cell

(Geng and Macdonald, 2006)

oogenesis stage S1 -- S4

nurse cell

(Munro et al., 2006)

oocyte

(Munro et al., 2006)

oogenesis stage S8 -- S14

oocyte | restricted

(Geng and Macdonald, 2006)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Immunolocalization shows that Imp is expressed in the -- and pole cells in blastoderm embryos and in the ventral nerve cord in stage 14-16 embryos. Double staining with an antibody to futsch reveals that Imp is expressed in peripheral sensory neurons and some futsch-negative cells surrounding them. Overlap with pros expression reveals Imp expression in the scolopale cells of chordotonal organs and in sheath cells of the external sensory organs. No staining was observed in peripheral motor axons or in muscle.

(Adolph et al., 2009)

Imp protein is cytoplasmic and present in all cells of the oocyte, follicle cells, nurse cells, and the oocyte. Within the germline, the pattern changes with time. It is initially uniform in the dividing germ line cells of each cyst but becomes rapidly concentrated in the oocyte. By stage S7, the uniform levels in the oocyte are noticably reduced but by stage S8 and S9 Imp levels are elevated in a narrow zone between the nucleus and the anterior and dorsolateral margins of the oocyte (an area which coincides with the region where grk mRNA and protein accumulate). This localizaton is lost by mid-stage 9. Imp also localizes to a crescent at the posterior pole of the oocyte.

(Geng and Macdonald, 2006)

Imp protein is found in the oocyte and in nurse cells early in oogenesis but penetration of the antibody into the oocyte is blocked after stage S4.

(Munro et al., 2006)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Medioni et al., 2014)

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in cytosol

inferred from direct assay

(Fic et al., 2019)

located_in neuronal cell body

inferred from direct assay

(Medioni et al., 2014)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

Reporter: P{lacW}130

Stage

Tissue/Position (including subcellular localization)

Reference

(Fabrizio et al., 2008)

Reporter: P{PTT-un}CB02137

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

follicle cell

(Nystul and Spradling, 2010)

Reporter: P{PTT-un}G00080

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

germarium

(Munro et al., 2006)

oogenesis stage S1 -- S7

oocyte | ubiquitous

(Munro et al., 2006)

oogenesis stage S8

oocyte | anterior

(Munro et al., 2006)

oocyte | posterior

(Munro et al., 2006)

oogenesis stage S9 -- S14

oocyte | posterior

(Munro et al., 2006)

Reporter: P{PTT-un}Imp^G0171X

Stage

Tissue/Position (including subcellular localization)

Reference

(Fabrizio et al., 2008)

(Fabrizio et al., 2008)

(Fabrizio et al., 2008)

Reporter: P{PTT-un}Imp^G0293X

Stage

Tissue/Position (including subcellular localization)

Reference

(Fabrizio et al., 2008)

(Fabrizio et al., 2008)

(Fabrizio et al., 2008)

Reporter: P{PTT-un}Imp^ZCL0310

Stage

Tissue/Position (including subcellular localization)

Reference

(Fabrizio et al., 2008)

(Fabrizio et al., 2008)

(Fabrizio et al., 2008)

Reporter: P{PTT-un}Imp^ZCL2884X

Stage

Tissue/Position (including subcellular localization)

Reference

(Fabrizio et al., 2008)

(Fabrizio et al., 2008)

adult brain cell body rind

(Fabrizio et al., 2008)

Reporter: PBac{802.P.SVS-2}Imp^CPTI003910

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

Comment: adult brain cortex, only around central brain

(Knowles-Barley, 2011.8.24)

Reporter: PBac{806.LOX-SVS-2}Imp^CPTI004117

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind | restricted

Comment: expression in part of the adult brain cortex surrounding the central brain

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Imp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Imp

View exonic expression by tissue for Dmel\Imp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32009/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0285926

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030235

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0285926

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 78 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Imp

Transgenic constructs containing regulatory region of Imp

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene not disrupted in

Df(1)N110

(Roote, 1999.10.28)

Gene disrupted in

Df(1)HC133

(Boylan et al., 2008, Roote, 1999.10.28)

Df(1)v64f

(Roote, 1999.10.28)

Gene duplicated in

Dp(1;Y)v⁺y⁺

(Boylan et al., 2008)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^elav.PU

Imp^RE.UASp

lethal, with Scer\GAL4^VP16.otu

Imp^HMS01168

lethal (with Df(1)HC133)

lethal | adult stage

Imp^G0072

lethal | embryonic stage | germline clone | maternal effect | recessive

Imp^H44

lethal | female limited, with Scer\GAL4^dome-PG14

Imp^HMS01168

lethal | pharate adult stage

Imp^G0072

lethal | pharate adult stage | recessive

Imp^H44

lethal | recessive

Imp^G0072

lethal - all die before end of P-stage, with Scer\GAL4^Act5C.PU

lethal - all die before end of P-stage | recessive

Imp^H149

lethal - all die during embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

Imp^SD.UASp

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Imp^GD9232

viable, with Scer\GAL4^Act5C.PU

Imp^KH.UASp

viable, with Scer\GAL4^elav.PU

viable, with Scer\GAL4^Mef2.PR

Imp^GD9232

viable, with Scer\GAL4^pnr-MD237

Imp^GD9232

viable, with Scer\GAL4^tin.CΔ4

Imp^GD9232

Sterility

Allele

female fertile, with Scer\GAL4^{VP16.mat.αTub67C}

fertile

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

Imp^RE.UASp

abnormal locomotor behavior | adult stage (with Imp^H44)

Imp^H149

abnormal locomotor behavior | adult stage (with Imp^H149)

Imp^H44

abnormal neuroanatomy

Imp⁸

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

abnormal neuroanatomy | adult stage

Imp^{G00080.CRISPR-ΔPLD}

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR16B06

abnormal neuroanatomy | adult stage | somatic clone

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

Imp^EP1433

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PU

Imp^RE.UASp

abnormal neuroanatomy | third instar larval stage (with Imp^H44)

Imp^H149

abnormal neuroanatomy | third instar larval stage (with Imp^H149)

Imp^H44

abnormal starvation stress response, with Scer\GAL4^elav.PU

Imp^GD9232

bang sensitive, with Scer\GAL4^nSyb.PP

Imp^HMC03794

decreased occurrence of cell division, with Scer\GAL4^pnt-14-94

Imp^HMS01168

increased cell number | adult stage, with Scer\GAL4^GMR16B06

Imp^UAS.cGa

long lived, with Scer\GAL4^elav.PU

Imp^GD9232

some die during embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

Imp^SD.UASp

some die during pharate adult stage, with Scer\GAL4^Act5C.PU

some die during pharate adult stage | recessive

Imp^H149

uncoordinated | adult stage, with Scer\GAL4^elav.PU

Imp^RE.UASp

uncoordinated | adult stage (with Imp^H44)

Imp^H149

uncoordinated | adult stage (with Imp^H149)

Imp^H44

Phenotype manifest in

Allele

abdominal dorsal multidendritic neuron ddaC | somatic clone | third instar larval stage

Imp⁸

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PG

adult ellipsoid body-protocerebral bridge 1 glomerulus-dorsal/ventral gall neuron, with Scer\GAL4^GMR16B06

Imp^HMS01168

adult ellipsoid body-protocerebral bridge 1 glomerulus-dorsal/ventral gall neuron | increased number, with Scer\GAL4^GMR16B06

Imp^UAS.cGa

adult protocerebral bridge 1 glomerulus-fan-shaped body-round body neuron, with Scer\GAL4^GMR16B06

adult round body, with Scer\GAL4^GMR16B06

Imp^HMS01168

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Imp^EP1433

dendritic tree | somatic clone | third instar larval stage

Imp⁸

dendritic tree | third instar larval stage, with Scer\GAL4^ppk.PG

dorsal appendage, with Scer\GAL4^{VP16.mat.αTub67C}

Imp^SD.UASp

egg, with Scer\GAL4^{VP16.mat.αTub67C}

Imp^UAS.cGa

egg chorion, with Scer\GAL4^{VP16.mat.αTub67C}

Imp^UAS.cGa

ellipsoid body, with Scer\GAL4^GMR16B06

Imp^HMS01168

fan-shaped body, with Scer\GAL4^GMR16B06

gall, with Scer\GAL4^GMR16B06

gamma Kenyon cell | adult stage

Imp^{G00080.CRISPR-ΔPLD}

gamma Kenyon cell | adult stage | somatic clone

macrochaeta

Imp^BG01822

NMJ bouton, with Scer\GAL4^elav.PU

Imp^RE.UASp

NMJ bouton (with Imp^H44)

Imp^H149

NMJ bouton (with Imp^H149)

Imp^H44

protocerebral bridge, with Scer\GAL4^GMR16B06

type II neuroblast | larval stage, with Scer\GAL4^pnt-14-94

Imp^HMS01168

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (29)

Hsap\IGF2BP1

14 of 14

Yes

Hsap\IGF2BP2

13 of 14

Yes

Hsap\IGF2BP3

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (26)

Rnor\Igf2bp1

14 of 14

Yes

Rnor\Igf2bp2

13 of 14

Yes

Rnor\Igf2bp3

12 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (25)

Mmus\Igf2bp1

14 of 14

Yes

Mmus\Igf2bp2

13 of 14

Yes

Mmus\Igf2bp3

12 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (24)

Xtro\igf2bp3

7 of 13

Yes

Xtro\aipl1

1 of 13

Yes

Xtro\aurkaip1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (29)

Drer\igf2bp1

14 of 14

Yes

Drer\igf2bp2a

13 of 14

Yes

Drer\igf2bp3

13 of 14

Yes

Drer\igf2bp2b

12 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\mex3a

1 of 14

Drer\mex3b

1 of 14

Drer\mib2

1 of 14

Drer\si:ch211-189e2.3

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\imph-1

11 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\vgln-1

1 of 14

Anopheles gambiae (African malaria mosquito) (9)

Agam\AgaP_AGAP012103

12 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (16)

Atha\AT1G14170

4 of 13

Yes

Atha\AT1G51580

4 of 13

Yes

Atha\AT4G18375

4 of 13

Yes

Atha\AT5G15270

4 of 13

Yes

Atha\HEN4

4 of 13

Yes

Atha\AT5G09560

3 of 13

Yes

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\HEK2

1 of 13

Yes

Scer\PBP2

1 of 13

Yes

Scer\SCP160

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\rnc1

1 of 12

Yes

Spom\snd1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Imp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (10)

HnRNP-K

5 of 13

mub

4 of 13

Psi

3 of 13

CG7878

1 of 13

CG11360

1 of 13

Dp1

1 of 13

papi

1 of 13

qin

1 of 13

tapas

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

diabetes mellitus type 2, susceptibility to, IGF2BP-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Imp^GD9232

model of acquired metabolic disease

CEA

(Kawasaki et al., 2015)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

IGF2BP2; insulin like growth factor 2 mRNA binding protein 2

model of type 2 diabetes mellitus

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Imp^GD9232

ameliorates brain cancer

modeled by pros^KK109284

(Narbonne-Reveau et al., 2016)

Imp^EP1433

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen and Bonini, 2007)

ameliorates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Bilen and Bonini, 2007)

Imp^HMS01168

ameliorates central nervous system cancer

modeled by Delta::N^ΔECN.UAS

(Voutyraki et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

IGF2BP1; insulin like growth factor 2 mRNA binding protein 1

14 of 14

INSULIN-LIKE GROWTH FACTOR 2 mRNA-BINDING PROTEIN 1; IGF2BP1

IGF2BP2; insulin like growth factor 2 mRNA binding protein 2

13 of 14

608289

TYPE 2 DIABETES MELLITUS; T2D

type 2 diabetes mellitus

IGF2BP3; insulin like growth factor 2 mRNA binding protein 3

13 of 14

608259

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Imp - mir-let7

immunohistochemistry, fluorescence technology, necessary binding region

(Toledano et al., 2012)

RNA-protein

Physical Interaction

Assay

References

Imp - InR

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Sreejith et al., 2022)

Imp - MED27

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Yang et al., 2017)

Imp - MED31

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Yang et al., 2017)

Imp - MED6

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Yang et al., 2017)

Imp - Sdc

anti tag coimmunoprecipitation, reverse transcription pcr

(Roy and Link, 2025)

Imp - chic

pull down, western blot, electrophoretic mobility shift assay, autoradiography, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Genovese et al., 2019, Medioni et al., 2014)

Imp - chinmo

pull down, western blot

(Genovese et al., 2019)

Imp - elav

clip-seq

(Carrasco et al., 2020)

Imp - grk

pull down, peptide massfingerprinting, filter binding, tag visualisation

(McDermott et al., 2012, Geng and Macdonald, 2006)

Imp - osk

filter binding, tag visualisation

(Geng and Macdonald, 2006)

Imp - pAbp

electrophoretic mobility shift assay, autoradiography

(Hansen et al., 2015)

Imp - sqd

electrophoretic mobility shift assay, autoradiography, anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(Hansen et al., 2015, Clouse et al., 2008, Geng and Macdonald, 2006)

Imp - upd1

anti tag coimmunoprecipitation, primer specific pcr, quantitative reverse transcription pcr, pull down, anti tag western blot

(Sreejith et al., 2019, Toledano et al., 2012)

protein-protein

Physical Interaction

Assay

References

Imp - CaMKII

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Formicola et al., 2021)

Imp - Dsor1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Imp - His2Av

pull down, western blot

(Dronamraju and Mason, 2009)

Imp - Hrb27C

anti bait coimmunoprecipitation, western blot

(Geng and Macdonald, 2006)

Imp - Mettl14

anti tag coimmunoprecipitation, anti tag western blot

(Tang et al., 2021)

Imp - Mettl3

anti tag coimmunoprecipitation, anti tag western blot

(Tang et al., 2021)

Imp - Mkrn1

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Dold et al., 2020)

Imp - Rheb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Imp - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Imp - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Imp - bsd

anti tag coimmunoprecipitation, anti tag western blot

(Yang et al., 2022)

Imp - fl(2)d

anti tag coimmunoprecipitation, anti tag western blot

(Tang et al., 2021)

Imp - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Imp - lin-28

anti tag coimmunoprecipitation, western blot, two hybrid, bimolecular fluorescence complementation, fluorescence microscopy

(Sreejith et al., 2022)

Imp - mu2

pull down, western blot

(Dronamraju and Mason, 2009)

Imp - nito

anti tag coimmunoprecipitation, anti tag western blot

(Tang et al., 2021)

Imp - orb2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(White-Grindley et al., 2014)

Imp - p53

pull down, autoradiography

(Lunardi et al., 2010)

Imp - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Imp - rl

anti tag coimmunoprecipitation, anti tag western blot

(Graeve et al., 2024)

Imp - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Imp - sqd

electrophoretic mobility shift assay, autoradiography, anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(Hansen et al., 2015, Clouse et al., 2008, Geng and Macdonald, 2006)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

chic

(Medioni et al., 2014)

enhanceable

CycE

(Geng and MacDonald, 2007)

enhanceable

E2f1

(Geng and MacDonald, 2007)

enhanceable

jigr1

(Geng and MacDonald, 2007)

enhanceable

l(2)SH1919

(Geng and MacDonald, 2007)

enhanceable

msi

(Geng and MacDonald, 2007)

enhanceable

mub

(Geng and MacDonald, 2007)

suppressible

Sdc

(Roy and Link, 2025)

enhanceable

sna

(Geng and MacDonald, 2007)

enhanceable

sqd

(Geng and MacDonald, 2007)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brat

suppressible

(Landskron et al., 2018)

Ets21C

suppressible

(Mundorf et al., 2019)

grim

suppressible

(Müller et al., 2005)

suppressible

(Müller et al., 2005)

hid

suppressible

(Bilen and Bonini, 2007, Müller et al., 2005)

suppressible

(Hattori et al., 2013)

p53

suppressible

(Müller et al., 2005)

Ppt1

enhanceable

(Saja et al., 2010)

rpr

suppressible