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General Information
Symbol
Dmel\hpRNA:CR18854
Species
D. melanogaster
Name
hairpin RNA:CR18854
Annotation Symbol
CR18854
Feature Type
FlyBase ID
FBgn0285991
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As

esi-1, hp-CG18854, esi-1.1, esi-1.2

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:9,787,294..9,790,745 [-]
Recombination map

2-36

RefSeq locus
NT_033779 REGION:9787294..9790745
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Molecular Function (GO)
    [Detailed GO annotations]
    Experimental Evidence
    -
    Predictions / Assertions
    -
    Summaries
    Gene Model and Products
    Number of Transcripts
    3
    Number of Unique Polypeptides
    0

    Please see the GBrowse view of Dmel\hpRNA:CR18854 or the JBrowse view of Dmel\hpRNA:CR18854 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 6.17

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Gene model reviewed during 5.47

    Antisense (in part): 3' portion overlaps IP3K1 on opposite strand.

    Supported by strand-specific RNA-Seq data.

    antisense_gene ; SO:0000077

    Pseudogene (in part): incorporates a pseudogene similar to CG4036; proximate; partial.

    Sequence Ontology: Class of Gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Polypeptides with Identical Sequences

     

    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\hpRNA:CR18854 using the Feature Mapper tool.

    External Data
    Crossreferences
    Linkouts
    Gene Ontology (0 terms)
    Molecular Function (0 terms)
    Terms Based on Experimental Evidence (0 terms)
    Terms Based on Predictions or Assertions (0 terms)
    Biological Process (0 terms)
    Terms Based on Experimental Evidence (0 terms)
    Terms Based on Predictions or Assertions (0 terms)
    Cellular Component (0 terms)
    Terms Based on Experimental Evidence (0 terms)
    Terms Based on Predictions or Assertions (0 terms)
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    microinjection
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    imaginal disc

    Comment: library contains gonads

    Additional Descriptive Data

    Tissue-specific RNA-seq reveals that hairpin RNA is dominantly expressed in testis and also accumulates in lbraries from mass-isolated imaginal discs containing larval gonads.

    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\hpRNA:CR18854 in GBrowse 2
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Images
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 2 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 3 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of hpRNA:CR18854
    Transgenic constructs containing regulatory region of hpRNA:CR18854
    Deletions and Duplications ( 1 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Phenotype manifest in
    Allele
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( None identified )
    No orthologies identified
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )
    No non-Drosophilid orthologies identified
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Paralogs (via DIOPT v7.1)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please see the Physical Interaction reports below for full details
      RNA-protein
      Physical Interaction
      Assay
      References
      RNA-RNA
      Physical Interaction
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map

      2-36

      Cytogenetic map
      Sequence location
      2L:9,787,294..9,790,745 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      30D1-30D1
      Limits computationally determined from genome sequence between P{lacW}zf30Ck02506&P{EP}undEP424 and P{lacW}l(2)k06416k06416&P{lacW}sopk04603
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (4)
      Genomic Clones (9)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (72)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        RNAi and Array Information
        Linkouts
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: hpRNA:CR18854 esi-1

        Source for database merge of

        Source for merge of: CG18854 CG13122

        Source for merge of: CR18854 esi-1

        Additional comments

        The CG18854 annotation can produce an approximately 400bp dsRNA through interaction of its 5' and 3' untranslated regions. These regions are represented in FlyBase by the "esi-1" gene.

        esi-1 consists of a duplex of more than 400bp separated by a large loop.

        Source for merge of CG18854 CG13122 was a shared cDNA ( date:010720 ).

        Other Comments

        Annotation CG18854 renamed CR18854 in release 5.24 of the genome annotation.

        The siRNAs derived from esi-1 all arise from one genomic strand.

        The Hrp59 protein binds preferentially to a subset of mRNAs, including CG18854 mRNA.

        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 17 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        RNAcentral - A comprehensive ncRNA sequence collection representing all ncRNA types from a broad range of organisms
        Other crossreferences
        KEGG Genes - Molecular building blocks of life in the genomic space.
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        Synonyms and Secondary IDs (14)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (47)