FlyBase Gene Report: Dmel\RpS12

General Information

Symbol

Dmel\RpS12

Species

D. melanogaster

Name

Ribosomal protein S12

Annotation Symbol

CG11271

Feature Type

protein_coding_gene

FlyBase ID

FBgn0286213

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Ribosomal protein S12 (RpS12) is an essential gene encoding a protein component of the cytoplasmic small ribosomal subunit. [Date last reviewed: 2019-03-28] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

l(3)s2783, su(comp)3L-1, S12

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:13,023,352..13,024,762 [+]

Recombination map

3-39

RefSeq locus

NT_037436 REGION:13023352..13024762

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from direct assay

(Anger et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from biological aspect of ancestor with PANTHER:PTN000217533

(assigned by GO_Central )

(Gaudet et al., 2011)

traceable author statement

(Marygold et al., 2007)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cytoplasmic translation

inferred by curator from GO:0022626

(Anger et al., 2013)

traceable author statement

(Marygold et al., 2007)

involved_in translation

inferred from electronic annotation with InterPro:IPR000530

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosolic ribosome

inferred from direct assay

(Anger et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of cytosolic small ribosomal subunit

inferred from biological aspect of ancestor with PANTHER:PTN000217533

(assigned by GO_Central )

(Gaudet et al., 2011)

traceable author statement

(Marygold et al., 2007)

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS

Protein Family (UniProt)

Belongs to the eukaryotic ribosomal protein eS12 family. (P80455)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Ribosomal protein S12 (RpS12) is an essential gene encoding a protein component of the cytoplasmic small ribosomal subunit. [Date last reviewed: 2019-03-28]

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)

Protein Function (UniProtKB)

Subunit of the 40S ribosomal complex (PubMed:23636399). In wing imaginal disks, might have a role in translation rate, growth and cell competition, probably through regulation of Xrp1 expression (PubMed:31841522). Might have a role in development and longevity (PubMed:31841522).

(UniProt, P80455)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RpS12 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

gene

(Tyler et al., 2007)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpS12-RB

FBtr0075878

NM_168534

613

139

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

RpS12-PB

FBpp0075612

15.2

139

6.34

NP_729866

AAN11845

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Subunit of the 40S ribosomal complex (PubMed:23636399).

(UniProt, P80455)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P80455

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS12 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 12 -- 16

embryonic/larval trachea

(Chung et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosolic ribosome

inferred from direct assay

(Anger et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RpS12 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpS12

View exonic expression by tissue for Dmel\RpS12

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB

39480/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0286213

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpS12

Transgenic constructs containing regulatory region of RpS12

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3L)ED4486

(Marygold et al., 2007)

Df(3L)VW1

(Marygold et al., 2007)

Inferred from location ( 0 )

Df(3L)ED4486

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | larval stage | somatic clone

RpS12^s2783

lethal | recessive

RpS12^s2783

lethal - all die before end of second instar larval stage (with RpS12^c06284)

lethal - all die before end of second instar larval stage (with RpS12^EP3025)

lethal - all die before end of second instar larval stage (with RpS12^s2783)

lethal - all die before end of second instar larval stage | recessive

viable

RpS12^G97D

viable (with RpS12^c06284)

RpS12^G97D

viable (with RpS12^EP3025)

RpS12^G97D

viable (with RpS12^G97D)

viable (with RpS12^s2783)

RpS12^G97D

Phenotype manifest in

Allele

wing disc | larval stage | somatic clone

RpS12^s2783

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RPS12

13 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\RSRC1

1 of 15

Yes

Hsap\SNU13

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rps12

11 of 15

Yes

Mmus\Gm4925

2 of 15

Yes

Mmus\Rps12-ps9

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Rps12-ps10

1 of 15

Yes

Mmus\Rps12-ps24

1 of 15

Yes

Mmus\Rsrc1

1 of 15

Yes

Mmus\Snu13

1 of 15

Rattus norvegicus (Norway rat) (12)

Rnor\Rps12

12 of 13

Yes

Rnor\LOC100360573

11 of 13

Yes

Rnor\RGD1561102

11 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\RGD1562265

1 of 13

Yes

Rnor\Rpl7a

1 of 13

Rnor\Rpp38

1 of 13

Yes

Rnor\Rps12l3

1 of 13

Yes

Rnor\Snu13

1 of 13

Xenopus tropicalis (Western clawed frog) (7)

Xtro\rps12

8 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\rsrc1

1 of 12

Yes

Xtro\snu13

1 of 12

Danio rerio (Zebrafish) (7)

Drer\rps12

13 of 15

Yes

Drer\nhp2

1 of 15

Drer\rpl7a

1 of 15

Drer\rpp38

1 of 15

Yes

Drer\rsrc1

1 of 15

Drer\snu13a

1 of 15

Drer\snu13b

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\rps-12

11 of 15

Yes

Cele\rpl-7A

1 of 15

Cele\Y48A6B.3

1 of 15

Cele\Y73B3A.18

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\AT1G15930

8 of 9

Yes

Atha\AT2G32060

8 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\RPS12

12 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\rps1201

10 of 12

Yes

Spom\rps1202

9 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190IG0 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10880

Drosophila persimilis

Dper\GL25268

Drosophila willistoni

Dwil\GK16839

Drosophila willistoni

Dwil\GK16837

Drosophila virilis

Dvir\GJ11353

Drosophila mojavensis

Dmoj\GI11681

Drosophila grimshawi

Dgri\GH23307

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150EV7 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17013144

Musca domestica

House fly

Mdoa\17025146

Glossina morsitans

Tsetse fly

Gmor\GMOY002168

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_05887

Mayetiola destructor

Hessian fly

Mdes\Mdes000025

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010299

Anopheles darlingi

American malaria mosquito

Adar\ADAC005191

Anopheles gambiae

Malaria mosquito

Agam\Rps12

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007993

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IGG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\RpS12

Danaus plexippus

Monarch butterfly

Dple\DPOGS213492-PA

Apis florea

Little honeybee

Aflo\4361037892

Apis mellifera

Western honey bee

Amel\GB51201

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22633

Bombus terrestris

Buff-tailed bumblebee

Bter\15214619

Linepithema humile

Argentine ant

Lhum\LH24703

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000f3c

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006871

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_04091

Tribolium castaneum

Red flour beetle

Tcas\TC009260

Pediculus humanus

Human body louse

Phum\PHUM204140

Rhodnius prolixus

Kissing bug

Rpro\RPRC013169

Cimex lectularius

Bed bug

Clec\CLEC003138

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000031

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_05370

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0IK0 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR001472

Ixodes scapularis

Black-legged tick

Isca\ISCW017361

Stegodyphus mimosarum

African social velvet spider

Smim\KFM73958.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur651g00010

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g00860

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0UB5 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rps12

Ciona intestinalis

Vase tunicate

Cint\LOC100184468

Gallus gallus

Domestic chicken

Ggal\RPS12

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPS12; ribosomal protein S12

13 of 15

603660

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpS12 - CG18787

experimental knowledge based

(Guruharsha et al., 2011)

RpS12 - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL28

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL37a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS15Aa

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS12 - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpS12 - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS12 - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lin et al., 2011)

enhanceable

(Kale et al., 2018)

External Data

Subunit Structure (UniProtKB)

Subunit of the 40S ribosomal complex (PubMed:23636399).

(UniProt, P80455 )

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-39

Cytogenetic map

69F5-69F5

Sequence location

3L:13,023,352..13,024,762 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

69F5-69F5

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

69C2-70D1

(Tyler et al., 2007)

69F5-69F6

(determined by in situ hybridisation)

(Spradling et al., 1999)

69F5-69F6

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-39

(FlyBase, 2016)

3-34.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

12085

w¹¹¹⁸; P{lacW}RpS12^s2783/TM3, Sb¹ Ser¹

22619

y¹ w^67c23; P{EPgy2}RpS12^EY23269/TM3, Sb¹ Ser¹

Kyoto

111390

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}RpS12^s2783 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹

VDRC

v109381

P{KK115942}VIE-260B

Genomic Clones (4)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (404)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kale et al., 2018)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: RpS12 l(3)s2783

(Spradling et al., 1999)

Source for merge of: RpS12 su(comp)3L-1

(Kale et al., 2018)

Additional comments

Release 4.3 annotation CG11271 split into two separate annotations in release 5.1 of the genome annotation, CG11271 (which corresponds to RpS12) and CG17672.

(FlyBase Genome Annotators, 2006)

Source for split of: CG11271

(Marygold, 2005.2.2)

It is not possible to say whether "M(3)69E" corresponds to "RpS12" or "RpS4", or whether the genetically-defined "M(3)69E" locus is a composite of both ribosomal protein genes.

(Deal and Cook, 2002.1.22)

Annotations CG11271 and CG17672 merged as CG11271 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

Not a Minute gene.

(Marygold et al., 2007)

Deletions removing RpS12 but no other cytoplasmic ribosomal protein-encoding genes do not show Minute phenotypes.

(Marygold et al., 2007)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 31 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39480

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P80455

Other crossreferences

DRscDB

39480/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0286213

InterPro - A database of protein families, domains and functional sites

MARRVEL_MODEL

39480

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

BcDNA:RH29995

CG11271

(Hallier et al., 2015, Rosa-Ferreira et al., 2015, Fukuoh et al., 2014, Schertel et al., 2013, Yoshigi et al., 2013, Cammarato et al., 2011, Keleman et al., 2009.8.5, Marygold et al., 2007, Huang et al., 2005, Marygold, 2005.2.2, Accardo et al., 2004)

RS12

(Mushegian et al., 1998)

RpS12

(Polymenis, 2020, Baker et al., 2019, Crofton et al., 2018, Kale et al., 2018, Rennie et al., 2018, Angrisani et al., 2015, Zabidi et al., 2015, Handler et al., 2013, Kwon et al., 2013, Schertel et al., 2013, Yoshigi et al., 2013, Chung et al., 2011, Lin et al., 2011, Parry et al., 2010, Marygold et al., 2007)

RpS12E

S12

(Wang et al., 2014, Brogna et al., 2002)

dRpS12

(Kale et al., 2018)

l(3)s2783

(Netter et al., 1998, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

rpS12

(Boulan et al., 2019, Ji et al., 2019)

su(comp)3L-1

(Titen and Golic, 2008, Tyler et al., 2007)

Name Synonyms

40S ribosomal component

(Ni et al., 2006)

40S ribosomal protein S12

(Greig, 1995.7.7)

Ribosomal Protein S12

(Ahrens et al., 2007)

Ribosomal Protein S12e

(Kale et al., 2018)

Ribosomal protein S12

(Cammarato et al., 2011, Xun et al., 2008, Cho et al., 2005)

Ribosomal protein gene 12

(Zabidi et al., 2015)

S12

(Hallier et al., 2015)

putative ribosomal protein S12

(Accardo et al., 2004)

ribosomal protein

(Hallier et al., 2015)

Secondary FlyBase IDs

FBgn0036339
FBan0011271
FBgn0011809
FBgn0064825
FBgn0260441
FBgn0014027
FBgn0086209

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (78)

Report Sections

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