General Information
Symbol
Dmel\Pld
Species
D. melanogaster
Name
Phospholipase D
Annotation Symbol
CG12110
Feature Type
FlyBase ID
FBgn0286511
Gene Model Status
Stock Availability
Enzyme Name (EC)
Phospholipase D (3.1.4.4)
Gene Snapshot
In progress.Contributions welcome.
Also Known As
dPLD
Genomic Location
Cytogenetic map
Sequence location
2R:6,242,004..6,255,266 [+]
Recombination map
2-55
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Experimental Evidence
Predictions / Assertions
Catalytic Activity (EC)
Experimental Evidence
A phosphatidylcholine + H(2)O = choline + a phosphatidate (3.1.4.4)
Predictions / Assertions
-
Summaries
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\Pld or the JBrowse view of Dmel\Pld for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.47
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0085997
4492
1278
FBtr0085995
4980
1278
FBtr0085996
4704
1278
FBtr0085999
4330
1278
FBtr0114492
4651
1364
FBtr0336520
4276
1260
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0085341
145.5
1278
8.06
FBpp0085339
145.5
1278
8.06
FBpp0085340
145.5
1278
8.06
FBpp0085343
145.5
1278
8.06
FBpp0112984
155.4
1364
8.05
FBpp0307592
143.5
1260
8.06
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1278 aa isoforms: Pld-PA, Pld-PB, Pld-PC, Pld-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
Dietary alcohols at low to moderate levels increase phosphatidylcholine-specific phospholipase D in larvae. Pld protein activity was enhanced by Ca2+ and by GTP-γS in vitro.
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pld using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (10 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001683
(assigned by InterPro )
Biological Process (7 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR016555
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR016555
(assigned by InterPro )
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pld in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 11 )
Transgenic Constructs ( 11 )
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
 
12 of 15
Yes
Yes
 
11 of 15
No
Yes
 
11 of 15
Yes
No
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
11 of 15
Yes
Yes
11 of 15
Yes
Yes
11 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (4)
10 of 13
Yes
Yes
10 of 13
Yes
Yes
7 of 13
No
Yes
7 of 13
Yes
No
Xenopus tropicalis (Western clawed frog) (4)
6 of 12
Yes
Yes
6 of 12
Yes
Yes
6 of 12
Yes
Yes
6 of 12
Yes
Yes
Danio rerio (Zebrafish) (8)
10 of 15
Yes
Yes
10 of 15
Yes
Yes
10 of 15
Yes
Yes
10 of 15
Yes
Yes
9 of 15
Yes
No
9 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
11 of 15
Yes
Yes
11 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (4)
7 of 9
Yes
Yes
7 of 9
Yes
Yes
6 of 9
No
Yes
6 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (2)
11 of 15
Yes
Yes
11 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
8 of 12
Yes
Yes
8 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900RE )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915010X )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Lucilia cuprina
Australian sheep blowfly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01B0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0190 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G017Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-55
    Cytogenetic map
    Sequence location
    2R:6,242,004..6,255,266 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    42A14-42A14
    Limits computationally determined from genome sequence between P{lacW}l(2)k09848k09848&P{EP}EP407 and P{lacW}geminink14019&P{PZ}Adf101349
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    42A16-42A19
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (9)
    Genomic Clones (20)
    cDNA Clones (34)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: Pld CG12110
      Source for database merge of
      Source for merge of: Pld PC-Pld
      Additional comments
      "Pld" may correspond to "Scim20". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim20" near/in "Pld" or "bin3".
      Source for identity of Pld CG12110 was sequence comparison ( date:010210 ).
      Other Comments
      Dietary alcohols stimulate the activity of phosphatidylcholine-specific phospholipase D in larvae.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 60 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (11)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (60)