FB2021_01, released Feb 18, 2021

Gene: Dmel\cass

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General Information
Symbol
Dmel\cass
Species
D. melanogaster
Name
cassowary
Annotation Symbol
CG6582
Feature Type
protein_coding_gene
FlyBase ID
FBgn0286781
Gene Model Status
Current
Stock Availability
9 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

Aac11, cas, l(2)k06710

Key Links
Genomic Location
Cytogenetic map
36C4-36F1
Sequence location
2L:17,474,912..17,477,888 [-]
Recombination map

2-53

RefSeq locus
NT_033779 REGION:17474912..17477888
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (3 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:Acn; FB:FBgn0263198
(Rigou et al., 2009)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in negative regulation of apoptotic process
inferred from mutant phenotype
(Morris et al., 2006)
inferred from genetic interaction with FLYBASE:E2f1; FB:FBgn0011766
(Morris et al., 2006)
inferred from genetic interaction with FLYBASE:Rbf; FB:FBgn0015799
(Morris et al., 2006)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in negative regulation of apoptotic process
inferred from sequence or structural similarity with HGNC:594
(FlyBase Curators, 2008--)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from sequence or structural similarity with HGNC:594
(FlyBase Curators, 2008--)
inferred from sequence or structural similarity with UniProtKB:Q9BZZ5
(assigned by UniProt )
(UniProt Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the API5 family. (Q9V431)
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Antiapoptotic factor. Also known to efficiently suppress E2F1-induced apoptosis.
(UniProt, Q9V431)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\cass for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene
gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
cass-RA
FBtr0081001
NM_058106
2458
536
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
cass-PA
FBpp0080554
59.9
536
6.74
NP_477454
AAF53618
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cass using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\cass in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of cass
Transgenic constructs containing regulatory region of cass
Aberrations (Deficiencies and Duplications) ( 12 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal (with Df(2L)BSC148)
lethal | recessive
lethal - all die before end of pupal stage | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal neuroanatomy | larval stage
abnormal pain response | adult stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
some die during pupal stage | recessive
visible, with cassdsRNA.UAS, Scer\GAL4Act88F.PD
visible, with cassdsRNA.UAS, Scer\GAL4ap-md544
visible, with cassdsRNA.UAS, Scer\GAL4en-e16E
visible, with cassk06710, Scer\GAL4Act88F.PD
visible, with cassk06710, Scer\GAL4ap-md544
visible, with cassk06710, Scer\GAL4en-e16E
visible, with Scer\GAL4Act88F.PD
visible, with Scer\GAL4ap-md544
visible, with Scer\GAL4en-e16E
visible | somatic clone
Phenotype manifest in
Allele
embryonic/first instar larval cuticle | dorsal
embryonic/larval somatic muscle
ganglion mother cell | larval stage
larval neuroblast | larval stage
wing | somatic clone
wing blade, with cassdsRNA.UAS, Scer\GAL4Act88F.PD
wing blade, with cassdsRNA.UAS, Scer\GAL4ap-md544
wing blade, with cassdsRNA.UAS, Scer\GAL4en-e16E
wing blade, with cassk06710, Scer\GAL4Act88F.PD
wing blade, with cassk06710, Scer\GAL4ap-md544
wing blade, with cassk06710, Scer\GAL4en-e16E
wing blade, with Scer\GAL4Act88F.PD
wing blade, with Scer\GAL4ap-md544
wing blade, with Scer\GAL4en-e16E
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\API5
14 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Api5
13 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Api5
13 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\api5
9 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\api5
11 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (4)
Atha\AT1G29030
9 of 9
Yes
Yes
Atha\AT2G34040
8 of 9
No
Yes
Atha\AT3G19515
3 of 9
No
Yes
Atha\AT1G23935
2 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091906JL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila simulans
Dsim\GD21866
Drosophila sechellia
Dsec\GM17125
Drosophila erecta
Dere\GG21743
Drosophila yakuba
Dyak\GE13131
Drosophila ananassae
Dana\GF15101
Drosophila pseudoobscura pseudoobscura
Dpse\GA19700
Drosophila persimilis
Dper\GL14497
Drosophila willistoni
Dwil\GK24861
Drosophila virilis
Dvir\GJ20327
Drosophila mojavensis
Dmoj\GI22431
Drosophila grimshawi
Dgri\GH10157
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915052U )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17014796
Glossina morsitans
Tsetse fly
Gmor\GMOY010426
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08888
Mayetiola destructor
Hessian fly
Mdes\Mdes008782
Mayetiola destructor
Hessian fly
Mdes\Mdes011218
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL014867
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012286
Anopheles darlingi
American malaria mosquito
Adar\ADAC007733
Anopheles gambiae
Malaria mosquito
Agam\AGAP009645
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ004812
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04MJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA001175
Danaus plexippus
Monarch butterfly
Dple\DPOGS207469-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL016681
Apis florea
Little honeybee
Aflo\2152281796
Apis mellifera
Western honey bee
Amel\GB47554
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP18146
Bombus terrestris
Buff-tailed bumblebee
Bter\15206404
Linepithema humile
Argentine ant
Lhum\LH22360
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000636
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG009137
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03894
Tribolium castaneum
Red flour beetle
Tcas\GLEAN_07927
Pediculus humanus
Human body louse
Phum\PHUM055720
Rhodnius prolixus
Kissing bug
Rpro\RPRC000493
Cimex lectularius
Bed bug
Clec\CLEC005990
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001216
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_06705
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04IZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR006832
Ixodes scapularis
Black-legged tick
Isca\ISCW002567
Stegodyphus mimosarum
African social velvet spider
Smim\KFM62222.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur12g00340
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_190896
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06NK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Api5
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_017264gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Aif_1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000023119
Gallus gallus
Domestic chicken
Ggal\API5
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    API5; apoptosis inhibitor 5
    14 of 15
    609774
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        cass - Acn
        two hybrid
        (Rigou et al., 2009)
        cass - Rab23
        anti tag coimmunoprecipitation, peptide massfingerprinting
        (Çiçek et al., 2016)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Dp
        enhanceable
        cass
        (Morris et al., 2006)
        E2f1
        enhanceable
        cass
        (Morris et al., 2006)
        if
        enhanceable
        cass
        (Walsh and Brown, 1998)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map

        2-53

        Cytogenetic map
        36C4-36F1
        Sequence location
        2L:17,474,912..17,477,888 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        36C4-36F1
        Left limit from inclusion within Df(2L)T317 (FBrf0104793) Right limit from inclusion within Df(2L)T317 (FBrf0104793)
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        36C8-36C11
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        The cass complementation group maps to the genomic interval 2L:17473293..17482011 , release 6 genome (left limit from complementation mapping against Df(2L)ED1161, right limit from complementation mapping against Df(2L)Exel7069).
        (Kahsai and Cook, 2018)
        36C8-36C11
        36D1--3
        (BDGP Project Members, 1994-1999)
        Experimentally Determined Recombination Data
        Location

        2-53

        (FlyBase, 2016)

        2-52.7

        (Comeron et al., 2012)

        2-

        (Prout et al., 1997)
        Left of (cM)
        Right of (cM)
        Notes

        Located on 2L.

        (Prout et al., 1997)
        Stocks and Reagents
        Stocks (9)
        Genomic Clones (16)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (174)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Morris et al., 2006)
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of
            Source for database merge of

            Source for merge of: cass Aac11

            (Kahsai and Cook, 2018)
            Additional comments
            Other Comments

            Identification: Genetic screen for mutations that produce blisters in somatic wing clones. 3 "bladderwrack" alleles have been isolated.

            (Walsh and Brown, 1998)

            Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones. 5 alleles of cass have been isolated.

            (Prout et al., 1997)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 16 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            InterPro - A database of protein families, domains and functional sites
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            Synonyms and Secondary IDs (15)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (55)