FlyBase Gene Report: Dmel\scb

General Information

Symbol

Dmel\scb

Species

D. melanogaster

Name

scab

Annotation Symbol

CG8095

Feature Type

protein_coding_gene

FlyBase ID

FBgn0286785

Gene Model Status

Current

Stock Availability

22 publicly available

Gene Snapshot

scab (scb) encodes the α-PS3 Integrin, believed to dimerise with mys and possibly Itgbn. It regulates adhesion, signalling, polarity and cell migration. Genetic interaction with Laminin genes implicates them as extracellular matrix ligands. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

αPS3, Volado, Vol, l(2)51Ea, α_PS3

Key Links

Genomic Location

Cytogenetic map

51E10-51E11

Sequence location

2R:15,248,784..15,258,498 [+]

Recombination map

2-74

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

INTEGRINS

Protein Family (UniProt)

Belongs to the integrin alpha chain family. (O44386)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

protein heterodimerization activity

Predictions / Assertions

Summaries

Gene Group (FlyBase)

INTEGRINS -: Integrins are heterodimeric transmembrane receptors composed of an α and β subunit that mediate cell-cell and cell-extracellular matrix adhesion. As well as maintaining tissue integrity, they are involved in morphogenesis and development. (Adapted from FBrf0167428).

Protein Function (UniProtKB)

Integrin alpha-PS3/beta-PS is a receptor for laminin. Also binds to wb. Important during embryogenesis for the development of the trachea, dorsal vessel and salivary gland, as well as for dorsal closure. Required for short-term memory processes. Minor involvement in the establishment of the oocyte anterior-posterior length. Plays a role in timely border cell migration during oogenesis, probably mediated by JNK signaling. Integrin alpha-PS3/Itgbn is required for effective phagocytosis of apoptotic cells during embryonic development and for the phagocytic elimination of S.aureus by mediating the binding of S.aureus peptidoglycan to larval hemocytes, which probably activates a signaling pathway involving Rac1 and Rac2. Integrin alpha-PS3/Itgbn also regulates Fak activity during neuromuscular junction (NMJ) growth and is required for its activation in presynapsis of NMJs. Seems to be dispensable for major morphogenetic processes.

(UniProt, O44386)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

scb: scab (C. Nusslein-Volhard)

Embryonic lethal. Embryos have small mid-dorsal hole with necrotic rim.

Summary (Interactive Fly)

α-PS3 integrin - involved in dorsal closure, tracheal and salivary gland development and in the localization of pericardial cells - thought to dimerize with Myospheroid and possibly Integrin betanu subunit - Scb regulates adhesion, signalling, polarity and cell migration - Genetic interaction with Laminin genes implicates them as extracellular matrix ligands.

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\scb or the JBrowse view of Dmel\scb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

scb-RA

FBtr0087370

NM_166083

4304

1115

scb-RB

FBtr0087369

NM_079026

4643

1115

Additional Transcript Data and Comments

Reported size (kB)

4.6, 4.4 (northern blot)

(Grotewiel et al., 1998)

4.9, 4.5 (northern blot)

(Stark et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

scb-PA

FBpp0086502

124.3

1115

5.50

NP_725445

AAF58156

scb-PB

FBpp0086501

124.5

1115

5.86

NP_523750

AAF58155

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

1115 (aa)

(Grotewiel et al., 1998)

1115 (aa); 90 (kD observed)

(Stark et al., 1997)

Comments

scb proteins contain a single transmembrane domain near the carboxy terminus. They differ only in the first 63 amino acids.

(Grotewiel et al., 1998)

External Data

Subunit Structure (UniProtKB)

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Interacts with mys/beta-PS and Itgbn.

(UniProt, O44386)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\scb using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Gene Ontology (33 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

protein heterodimerization activity

inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657

(Stark et al., 1997)

inferred from physical interaction with FLYBASE:Itgbn; FB:FBgn0010395

(Nonaka et al., 2013)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (25 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

apoptotic cell clearance

inferred from mutant phenotype

(Nonaka et al., 2013)

axon guidance

inferred from mutant phenotype

(Stevens and Jacobs, 2002)

behavioral response to ethanol

inferred from mutant phenotype

(Kong et al., 2010)

dorsal closure

inferred from mutant phenotype

(Wada et al., 2007, MacMullin and Jacobs, 2006, Stark et al., 1997)

dorsal trunk growth, open tracheal system

inferred from mutant phenotype

(Stark et al., 1997)

larval heart development

inferred from mutant phenotype

(Vanderploeg et al., 2012)

negative regulation of cell migration

inferred from mutant phenotype

(assigned by UniProt )

(Dinkins et al., 2008)

negative regulation of synaptic growth at neuromuscular junction

inferred from genetic interaction with FLYBASE:Fak; FB:FBgn0020440

(Tsai et al., 2008)

oocyte growth

inferred from mutant phenotype

(assigned by UniProt )

(Dinkins et al., 2008)

pericardium morphogenesis

inferred from mutant phenotype

(Stark et al., 1997)

phagocytosis

inferred from mutant phenotype

(Nonaka et al., 2013)

salivary gland development

inferred from mutant phenotype

(Stark et al., 1997)

wound healing

inferred from mutant phenotype

(Campos et al., 2010)

Terms Based on Predictions or Assertions (13 terms)

CV Term

Evidence

References

calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

calcium-dependent cell-matrix adhesion

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

cell adhesion mediated by integrin

inferred from sequence or structural similarity with FLYBASE:mys; FB:FBgn0004657

(FlyBase Curators, 2008--)

cell migration

traceable author statement

(Brown, 2000)

cell-matrix adhesion

traceable author statement

(Hynes and Zhao, 2000)

dorsal closure

traceable author statement

(Harden, 2002, Lamka and Lipshitz, 1999, Noselli and Agnes, 1999)

heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules

traceable author statement

(Hynes and Zhao, 2000)

learning or memory

non-traceable author statement

(Sokolowski, 2001)

locomotion

non-traceable author statement

(Sokolowski, 2001)

memory

traceable author statement

(Waddell and Quinn, 2001)

non-traceable author statement

(Roman and Davis, 2001)

midgut development

traceable author statement

(Brown, 2000)

olfactory learning

non-traceable author statement

(Roman and Davis, 2001)

short-term memory

traceable author statement

(Burrell and Sahley, 2001)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

apical plasma membrane

inferred from direct assay

(assigned by UniProt )

(Dinkins et al., 2008)

basal plasma membrane

inferred from direct assay

(assigned by UniProt )

(Dinkins et al., 2008)

cytoplasm

inferred from direct assay

(assigned by UniProt )

(Dinkins et al., 2008)

integral component of membrane

inferred from direct assay

(Nonaka et al., 2013)

integrin complex

inferred from direct assay

(Nonaka et al., 2013)

inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657

(Stark et al., 1997)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

focal adhesion

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

integrin complex

inferred from sequence or structural similarity

(Brown, 2000, Hynes and Zhao, 2000)

non-traceable author statement

(Brower, 2003, Noselli and Agnes, 1999)

plasma membrane

inferred from sequence or structural similarity

(Stark et al., 1997)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

epithelial cell

• cytoplasm | apical

Comment: clustered apical distribution

(Lecuyer et al., 2007)

epithelium

• cytoplasm | apical

(Lecuyer et al., 2007)

pre-blastoderm stage

organism | ubiquitous

(Stark et al., 1997)

embryonic stage 7

amnioproctodeal invagination

(Stark et al., 1997)

embryonic stage 8 -- 14

amnioserosa

(Stark et al., 1997)

embryonic stage 9

tracheal pit

(Stark et al., 1997)

visceral mesoderm | presumptive

(Stark et al., 1997)

embryonic stage 11

posterior midgut primordium

(Stark et al., 1997)

anterior midgut primordium

(Stark et al., 1997)

embryonic stage 11 -- 15

presumptive embryonic salivary gland

(Bradley et al., 2003)

embryonic stage 13

proventriculus anlage

(Stark et al., 1997)

embryonic stage 13 -- 16

posterior midline glial cell

(Kearney et al., 2004)

embryonic stage 13 -- 17

embryonic/larval midgut

(Stark et al., 1997)

embryonic/larval salivary gland

(Stark et al., 1997)

embryonic stage 15

embryonic/larval dorsal vessel

(Stark et al., 1997)

embryonic stage 15 -- 16

mesectoderm

(Stark et al., 1997)

embryonic stage 15 -- 17

antenno-maxillary complex

(Stark et al., 1997)

embryonic stage 16

embryonic midgut constriction

(Stark et al., 1997)

ventral nerve cord | restricted

(Stark et al., 1997)

embryonic/larval salivary gland duct

(Stark et al., 1997)

embryonic stage 17

gastric caecum

(Stark et al., 1997)

embryonic/larval transverse connective

(Stark et al., 1997)

embryonic/larval dorsal trunk

(Stark et al., 1997)

embryonic/larval anterior spiracle

(Stark et al., 1997)

oogenesis stage S10A

border follicle cell

(Dinkins et al., 2008)

nurse follicle cell

(Dinkins et al., 2008)

oogenesis stage S10B -- S13

dorsal appendage forming follicle cell

(Dinkins et al., 2008)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Stark et al., 1997)

embryonic stage & pupal stage & adult stage

(Stark et al., 1997)

Additional Descriptive Data

scb appears in border cells and in stretch cells at oogenesis stage 10A. It is also strongly expressed in cells that will form the dorsal appendages at stages 10B and 11 and persists until stage 13.

(Dinkins et al., 2008)

scb transcript was first detected in late stage 11 embryonic salivary gland after initiation of mew expression. Low levels of expression persisted in all salivary gland cells until stage 15, at which time scb was expressed at a high level.

(Bradley et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunodetection (other than il)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval hemocyte

(Nonaka et al., 2013)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S10B -- S11

dorsal appendage forming follicle cell

(Dinkins et al., 2008)

oogenesis stage S10B -- S14

nurse follicle cell

(Dinkins et al., 2008)

oogenesis stage S13

oocyte associated follicle cell

(Dinkins et al., 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

Comment: highest levels in pupae

(Nonaka et al., 2013)

Additional Descriptive Data

The α3 integrin scb is generally enriched along the interface between the muscle layer and the epithelium of the midgut.

(Lin et al., 2013)

Co-localization with a hemocyte GFP marker shows that scb is present on the surface of embryonic hemocytes. A developmental western blot shows the hightest levels of scb protein in pupae and the lowest levels in larvae.

(Nonaka et al., 2013)

scb protein is observed starting in mid-oogenesis in dorsal appendage forming follicle cells, in stretch follicle cells, and in a posterior patch of follicle cells overlying the oocyte.

(Dinkins et al., 2008)

scb protein is concentrated in the mushroom body (corpora pedunculata) perikarya, calyces, peduncles and α, β, and γ lobes in adults. It is also observed in the ellipsoid body.

(Grotewiel et al., 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

apical plasma membrane

inferred from direct assay

(assigned by UniProt )

(Dinkins et al., 2008)

basal plasma membrane

inferred from direct assay

(assigned by UniProt )

(Dinkins et al., 2008)

cytoplasm

inferred from direct assay

(assigned by UniProt )

(Dinkins et al., 2008)

integral component of membrane

inferred from direct assay

(Nonaka et al., 2013)

integrin complex

inferred from direct assay

(Nonaka et al., 2013)

inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657

(Stark et al., 1997)

Expression Deduced from Reporters

Reporter: P{GawB}scb^NP7060

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

inner layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

outer layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

core layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\scb in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\scb

View exonic expression by tissue for Dmel\scb

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8095-RB

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 37 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of scb

Transgenic constructs containing regulatory region of scb

Deletions and Duplications ( 17 )

Not disrupted in

Df(2R)03072

(Brumby et al., 2004)

Df(2R)BSC651

(Kahsai and Cook, 2018)

Df(2R)Exel6285

(Kahsai and Cook, 2018)

Df(2R)Jp4

(Brumby et al., 2004, Sekelsky et al., 2000)

Df(2R)knSA3

(Sekelsky et al., 2000, Van Daele, 1998.8.11)

Df(2R)l13

(Underwood et al., 1990)

Df(2R)XTE-58

(Underwood et al., 1990)

Df(2R)XTED1

(Underwood et al., 1990)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

scb^{MI03836-GFSTF.2}

lethal (with Df(2R)BSC346)

scb²

lethal | heat sensitive

scb^fs14

lethal | recessive

lethal - all die before end of embryonic stage

lethal - all die before end of embryonic stage | recessive

lethal - all die before end of larval stage

lethal - all die before end of larval stage | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

scb^GD2006

partially lethal - majority die

scb⁰¹²⁸⁸

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

scb^GD2006

viable, with Scer\GAL4^Mef2.PR

scb^GD2006

Sterility

Allele

female sterile

scb^fs14

fertile

Other Phenotypes

Allele

chemical resistant

scb^NP7060

decreased cell number | adult stage | somatic clone

hyperactive | conditional

scb^NP7060

learning defective | dominant

learning defective | recessive

scb^Vol-1

learning defective | semidominant

memory defective

memory defective | dominant

neuroanatomy defective

scb^unspecified

neuroanatomy defective (with Df(2R)Jp1)

scb^unspecified

neuroanatomy defective (with Df(2R)Jp4)

scb^unspecified

neuroanatomy defective (with Df(2R)XTE-18)

scb^unspecified

neuroanatomy defective | larval stage

neuroanatomy defective | larval stage, with Scer\GAL4^elav-C155

scb^EP2591

neuroanatomy defective | third instar larval stage, with Scer\GAL4^repo

neurophysiology defective

neurophysiology defective | larval stage

size defective | adult stage | somatic clone

some die during embryonic stage

some die during embryonic stage | recessive

some die during larval stage

some die during larval stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

scb^GD2006

visible | somatic clone

scb¹

wild-type

scb^s.hs

Phenotype manifest in

Allele

adult posterior midgut epithelium | somatic clone

amnioserosa

scb^Vol-3

anterior fascicle & synapse, with Scer\GAL4^elav-C155

scb^EP2591

axon terminus | supernumerary

bouton | supernumerary

cell leading edge

scb^Vol-3

dorsal superficial trachea

embryo | dorsal

scb^unspecified

embryo | dorsal closure stage

scb⁰¹²⁸⁸

embryo | embryonic stage 14

scb^Vol-3

embryo | germline clone

embryo | germline clone (with Df(2R)Jp1)

scb²

embryo | germline clone (with scb²)

scb^5J38

embryo | germline clone (with scb^5J38)

scb²

embryonic/first instar larval cuticle

scb¹

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

embryonic/larval optic stalk | third instar larval stage, with Scer\GAL4^repo

embryonic/larval salivary gland | embryonic stage

embryonic dorsal epidermis

embryonic heart cardioblast

scb²

embryonic heart cardioblast, with Scer\GAL4^Mef2.PR

scb^KK106326

embryonic hemocyte, with Scer\GAL4^srp.Hemo

scb^NIG.8095R

extracellular matrix | third instar larval stage, with Scer\GAL4^repo

scb^JF02696

eye disc | third instar larval stage, with Scer\GAL4^repo

eye photoreceptor cell | third instar larval stage, with Scer\GAL4^repo

fascicle

scb^unspecified

fascicle (with Df(2R)Jp1)

scb^unspecified

fascicle (with Df(2R)Jp4)

scb^unspecified

fascicle (with Df(2R)XTE-18)

scb^unspecified

filamentous actin

scb^Vol-3

germ band | germline clone

germ band | germline clone (with Df(2R)Jp1)

scb²

germ band | germline clone (with scb²)

scb^5J38

germ band | germline clone (with scb^5J38)

scb²

intersegmental nerve, with Scer\GAL4^elav-C155

scb^EP2591

mitotic domain 1 | embryonic stage 13

scb^Vol-3

mitotic domain 1 | embryonic stage 15

scb^Vol-3

mitotic domain 1 | extended germ band stage

neuromuscular junction

pericardial cell

tracheal branch primordium

scb^Vol-3

wing vein | dorsal | somatic clone

scb¹

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (15)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ITGA4

5 of 15

Yes

Hsap\ITGA9

4 of 15

Hsap\ITGA1

3 of 15

Hsap\ITGA2

3 of 15

Yes

Hsap\ITGA10

3 of 15

Yes

Hsap\ITGA11

3 of 15

Yes

Hsap\ITGAD

3 of 15

Yes

Hsap\ITGAE

3 of 15

Yes

Hsap\ITGAL

3 of 15

Yes

Hsap\ITGAM

3 of 15

Yes

Hsap\ITGAX

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (15)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Itga4

7 of 15

Yes

Mmus\Itga9

5 of 15

Yes

Mmus\Itga1

3 of 15

Yes

Mmus\Itga2

3 of 15

Yes

Mmus\Itga11

3 of 15

Yes

Mmus\Itgad

3 of 15

Yes

Mmus\Itgae

3 of 15

Yes

Mmus\Itgal

3 of 15

Yes

Mmus\Itgam

3 of 15

Yes

Mmus\Itgax

3 of 15

Yes

Mmus\Itga10

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (15)

Rnor\Itga9

4 of 13

Yes

Rnor\Itga1

3 of 13

Yes

Rnor\Itga4

3 of 13

Yes

Rnor\Itga10

3 of 13

Yes

Rnor\Itga11

3 of 13

Yes

Rnor\Itgad

3 of 13

Yes

Rnor\Itgax

3 of 13

Yes

Rnor\Itga2

2 of 13

Yes

Rnor\Itgae

2 of 13

Yes

Rnor\Itgal

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Itgav

1 of 13

Xenopus tropicalis (Western clawed frog) (5)

Xtro\itga4

3 of 12

Yes

Xtro\itga9

3 of 12

Yes

Xtro\itga2

2 of 12

Yes

Xtro\itga11

2 of 12

Yes

Xtro\itga1

1 of 12

Yes

Danio rerio (Zebrafish) (16)

Drer\itga4

4 of 15

Yes

Drer\itga1

3 of 15

Yes

Drer\itga9

3 of 15

Yes

Drer\itga10

3 of 15

Yes

Drer\itga11a

3 of 15

Yes

Drer\itga11b

3 of 15

Yes

Drer\si:dkey-32n7.4

3 of 15

Yes

Drer\itga2.2

2 of 15

Yes

Drer\itgae.1

2 of 15

Yes

Drer\itgae.2

2 of 15

Yes

Drer\zmp:0000001082

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\itgav

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\pat-2

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919019R )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\scb

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20821

Drosophila persimilis

Dper\GL11087

Drosophila willistoni

Dwil\GK20889

Drosophila virilis

Dvir\GJ14970

Drosophila mojavensis

Dmoj\GI19606

Drosophila grimshawi

Dgri\GH20423

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150110 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17004559

Musca domestica

House fly

Mdoa\17004560

Glossina morsitans

Tsetse fly

Gmor\GMOY001582

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10562

Mayetiola destructor

Hessian fly

Mdes\Mdes008603

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013381

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011038

Anopheles gambiae

Malaria mosquito

Agam\AGAP006826

Anopheles gambiae

Malaria mosquito

Agam\AGAP004303

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ014514

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0776 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR006224

Strigamia maritima

European centipede

Smar\SMAR014703

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

2 of 10

mew

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Alzheimer disease, susceptibility to (postulated), ITGAM-ITGA9-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ITGA2; integrin subunit alpha 2

model of platelet-type bleeding disorder 9

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ITGA4; integrin subunit alpha 4

5 of 15

192975

ITGA9; integrin subunit alpha 9

4 of 15

603963

ITGA1; integrin subunit alpha 1

3 of 15

192968

ITGA2; integrin subunit alpha 2

3 of 15

192974

BLEEDING DISORDER, PLATELET-TYPE, 9; BDPLT9

platelet-type bleeding disorder 9

ITGA10; integrin subunit alpha 10

3 of 15

604042

ITGA11; integrin subunit alpha 11

3 of 15

604789

ITGAD; integrin subunit alpha D

3 of 15

602453

ITGAE; integrin subunit alpha E

3 of 15

604682

ITGAL; integrin subunit alpha L

3 of 15

153370

ITGAM; integrin subunit alpha M

3 of 15

120980

ITGAX; integrin subunit alpha X

3 of 15

151510

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

scb - Ilp8

pull down, Identification by mass spectrometry

(Garelli et al., 2015)

scb - Itgbn

anti bait coimmunoprecipitation, western blot

(Nonaka et al., 2013)

scb - mys

anti bait coimmunoprecipitation, protein sequence identification

(Stark et al., 1997)

scb - tkv

anti tag coimmunoprecipitation, anti tag western blot

(Sawala et al., 2015)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

scb

suppressible

LanA

(Stevens and Jacobs, 2002)

scb

enhanceable

LanA|sli

(Stevens and Jacobs, 2002)

scb

enhanceable

sli

(MacMullin and Jacobs, 2006, Stevens and Jacobs, 2002)

scb

enhanceable

sog

(Araujo et al., 2003)

scb

enhanceable

(Schock and Perrimon, 2003)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

CycE

enhanceable

scb

(Lane et al., 2000)

CycE

suppressible

scb

(Brumby et al., 2004)

LanA

enhanceable

scb|sli

(Stevens and Jacobs, 2002)

LanA

suppressible

scb

(Stevens and Jacobs, 2002)

sli

enhanceable

scb

(MacMullin and Jacobs, 2006, Stevens and Jacobs, 2002)

sli

enhanceable

LanA|scb

(Stevens and Jacobs, 2002)

sog

enhanceable

scb

(Araujo et al., 2003)

enhanceable

scb

(Schock and Perrimon, 2003)

External Data

Subunit Structure (UniProtKB)

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Interacts with mys/beta-PS and Itgbn.

(UniProt, O44386 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62429

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

36692

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-74

Cytogenetic map

51E10-51E11

Sequence location

2R:15,248,784..15,258,498 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

51E10-51E11

Limits computationally determined from genome sequence between P{PZ}chn⁰²⁰⁶⁴ and P{PZ}scb⁰¹²⁸⁸; Left limit from non-inclusion within Df(2R)XTE-58 (FBrf0051964) Right limit from inclusion within Df(2R)l4 (FBrf0051964)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

51E7-51E11

(Stark, 1996.10.28)

The l(2)51Ea complementation group maps to the genomic interval 2R:15218008..15262942 , release 6 genome (left limit from complementation mapping against Df(2R)BSC346, right limit from complementation mapping against Df(2R)Exel7135).

(Kahsai and Cook, 2018)

51E-51E

(Grotewiel, 1997.11.13, Grotewiel, 1997.11.13)

51E10-51E11

(determined by in situ hybridisation)

(Spradling et al., 1999)

51E-51E

(determined by in situ hybridisation)

(Grotewiel et al., 1998)

51E7-51E11

(determined by in situ hybridisation)

(Stark et al., 1997)

51E-51F

(Gotwals et al., 1994)

51E10-51E11

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-74

(FlyBase, 2016)

2-77.8

(Comeron et al., 2012)

(Lane et al., 2000)

2-73

(Tearle and Nusslein-Volhard, 1987)

2-[73]

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (22)

Bloomington

9408

scb^Vol-2/CyO

68157

P{neoFRT}42D scb²/CyO

6483

cn¹ scb^l7 bw¹/CyO

6484

cn¹ scb^l19 bw¹/CyO

27545

y¹ v¹; P{TRiP.JF02696}attP2

17655

y¹ w^67c23; P{EPgy2}EY10270/CyO

62707

w¹¹¹⁸; PBac{IT.GAL4}scb^0174-G4

Harvard

f06320

PBac{WH}scb^f06320

Kyoto

104772

w^*; P{GawB}scb^NP4749 / CyO

112656

y^* w^*; P{GawB}scb^NP1373

VDRC

v100949

P{KK106326}VIE-260B

v4891

w¹¹¹⁸; P{GD2006}v4891

More stocks available...

Genomic Clones (26)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (70)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Meehan et al., 2015, Nonaka et al., 2013, Grotewiel et al., 1998)

monoclonal

(Gullberg et al., 1994)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: scb E(Sev-CycE)E93

(Brumby et al., 2004)

Source for merge of: scb l(2)51Ea

(Kahsai and Cook, 2018)

Additional comments

Other Comments

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

scb regulates functional synaptic plasticity processes.

(Rohrbough et al., 2000)

mew and scb are required in a partially redundant way in the endoderm for normal migration of the endodermal midgut cells over the visceral mesoderm in the developing embryo.

(Martin-Bermudo et al., 1999)

Analysis suggests the scb integrin is essential for the physiological processes underlying memory. scb integrin mediates a dynamic process.

(Grotewiel et al., 1998)

scb mutants display impaired olfactory memories within 3 minutes of training, indicating that the encoded α-integrin is required for short-term memory processes. Conditional expression of a scb transgene during adulthood rescues the memory impairment. Rescue is reversible, fading over time along with expression of the transgene.

(Grotewiel et al., 1998)

Data suggests that scb might be the gene encoding αInt3.

(Stark et al., 1997)

The scb integrin, a cell surface protein predicted to mediate selective adhesion in response to activation by intracellular or extracellular signals, may participate in cell-cell interactions between mushroom bodies and these large interneurons underlying learning.

(Zhu et al., 1995)

The distribution of mew, if and scb proteins in cultured cell lines has been investigated.

(Peel and Milner, 1992)

Origin and Etymology

Discoverer

Etymology

The gene has been known as 'Volado', a Chilean colloquialism with no English counterpart, which is loosely translated as 'forgetful' or 'absent-minded'. In Chile, it is often used in reference to professors and scientists.

(Grotewiel et al., 1998)

Identification

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36692

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O44386

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

BioGRID - A database of protein and genetic interactions.

62429

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8095-RB

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

36692

Synonyms and Secondary IDs (36)

Reported As

Symbol Synonym

CG8095

(Chen et al., 2012, Christis and Munro, 2012, Ni et al., 2010.12.1, Zhang et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Schiess et al., 2009, Quinones-Coello, 2007, Brown and Feder, 2005, Kraut et al., 2001, Kraut et al., 2001)

CT21280

(Hynes and Zhao, 2000)

CT36929

(Hynes and Zhao, 2000)

Dm0620

(Harr and Schlotterer, 2000)

E(Sev-CycE)E93

(Lane et al., 2000)

E(sev-cycE)^e93

(Brumby et al., 2004)

ITA3

(Garelli et al., 2015)

ItgaPS3

(Brown et al., 2013.2.5)

PS3

(MacMullin and Jacobs, 2005, Peel and Milner, 1992)

Vol

(Davis, 2001, Roman and Davis, 2001, Beck et al., 2000, Rohrbough et al., 2000, Grotewiel et al., 1998, Mayford, 1998, Murthy, 1998, Sheng, 1998)

aPS3

(Agnes and Noselli, 1999)

alphaPS3

(Brown, 2000.7.20)

l(2)01288

l(2)51Ea

(Kahsai and Cook, 2018)

scab

(Park et al., 2018, Schwartz et al., 2016, Thimgan et al., 2013, Gates, 2012, Kaun et al., 2012, LeMosy and Dinkins, 2008, Stofanko et al., 2008)

scb

(Meltzer et al., 2019, Wittes and Schüpbach, 2019, Xu et al., 2019, Kahsai and Cook, 2018, Mortensen et al., 2018, Park et al., 2017, Clandinin and Owens, 2016-, Gene Disruption Project members, 2015-, Grotewiel and Bettinger, 2015, Sawala et al., 2015, Tomita et al., 2015, Van Bortle et al., 2015, Kim and Choe, 2014, Shulman et al., 2014, Brown et al., 2013.2.5, Lin et al., 2013, Nonaka et al., 2013, Robinson and Atkinson, 2013, Thimgan et al., 2013, Gates, 2012, Japanese National Institute of Genetics, 2012.5.21, Kaun et al., 2012, Vanderploeg et al., 2012, Knox et al., 2011, Campos et al., 2010, Kong et al., 2010, Bhandari et al., 2009, Krejcí et al., 2009, Dinkins et al., 2008, Stofanko et al., 2008, Tsai et al., 2008, Yang et al., 2008, Zhang et al., 2008, Christensen and Cook, 2007.10.29, Christensen et al., 2007.10.29, Lecuyer et al., 2007, Peralta et al., 2007, Quinones-Coello, 2007, Homsy et al., 2006, Stroschein-Stevenson et al., 2006)

vol

(Acevedo et al., 2007, Yamazaki et al., 2007, Tamura et al., 2003, Billuart et al., 2001, Waddell and Quinn, 2001)

α-PS3

(Hoang et al., 2002)

α_PS3

(Bradley et al., 2003, Martin-Bermudo et al., 1999)

αInt3

αPS3

(Park et al., 2018, Nainu et al., 2017, Wood and Martin, 2017, Ashe, 2016, Meehan et al., 2016, Kohlmaier et al., 2015, Sawala et al., 2015, Tavares et al., 2015, Xie et al., 2014, Lin et al., 2013, Nonaka et al., 2013, Bulgakova et al., 2012, Vanderploeg et al., 2012, Knox et al., 2011, Meghana et al., 2011, Urbano et al., 2011, Xie and Auld, 2011, Delon and Brown, 2009, Krejcí et al., 2009, Dinkins et al., 2008, Takada et al., 2007, Tanentzapf et al., 2007, Wada et al., 2007, Lo, 2006, MacMullin and Jacobs, 2006, Devenport and Brown, 2004, Huelsmann et al., 2004, Abrams et al., 2003, Brower, 2003, Schock and Perrimon, 2003, Bokel and Brown, 2002, Stevens and Jacobs, 2002, Araujo et al., 2001, Hoang et al., 2001, Hughes, 2001, Brown, 2000, Brown et al., 2000, D'Avino and Thummel, 2000, Hynes and Zhao, 2000, Stark et al., 1997, Gotwals et al., 1994)

β_PS3

(Gullberg et al., 1994)

Name Synonyms

Complementation group 2.11

(Brumby et al., 2004)

Integrin alphaPS3 subunit

(Brown et al., 2013.2.5)

Scab

(Brown and Feder, 2005)

Volado

(Jin, 2002, Kraut et al., 2001, Kraut et al., 2001, Morley and Montgomery, 2001, Davis, 2000, Zars, 2000, Murase and Schuman, 1999, Anonymous, 1998, Connolly and Tully, 1998, Grotewiel, 1997.11.13, Grotewiel, 1997.11.13, Grotewiel et al., 1997)

aVolado

(Prokop et al., 1997)

alphaPS3 subunit

(LeMosy and Dinkins, 2008)

integrin αPS3 subunit

(Brown et al., 2013.2.5)

laminin receptor integrin α-PS3

(Garelli et al., 2015)

lethal (2) 51Ea

volado

(Yamazaki et al., 2007, Dubnau et al., 2003, Wolf and Heberlein, 2003, Burrell and Sahley, 2001, Waddell and Quinn, 2001, Waddell and Quinn, 2001, Beumer et al., 1999, Anonymous, 1998, Prokop et al., 1997, Zhu et al., 1995)

α Position-Specific antigen 3

α3 Integrin subunit

(Pasco et al., 2015)

αPS3 integrin

(Lo, 2006)

βPS3integrin

(Gullberg et al., 1994)

Secondary FlyBase IDs

FBgn0003328
FBgn0010481
FBgn0011707
FBgn0013742
FBgn0014404
FBgn0041840
FBgn0004158

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (228)

Report Sections

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