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General Information
Symbol
Dmel\scb
Species
D. melanogaster
Name
scab
Annotation Symbol
CG8095
Feature Type
FlyBase ID
FBgn0286785
Gene Model Status
Stock Availability
Gene Snapshot
scab (scb) encodes the α-PS3 Integrin, believed to dimerise with mys and possibly Itgbn. It regulates adhesion, signalling, polarity and cell migration. Genetic interaction with Laminin genes implicates them as extracellular matrix ligands. [Date last reviewed: 2019-03-14]
Also Known As
αPS3, Volado, Vol, l(2)51Ea, αPS3
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:15,248,784..15,258,498 [+]
Recombination map
2-74
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the integrin alpha chain family. (O44386)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
Predictions / Assertions
-
Summaries
Gene Group (FlyBase)
INTEGRINS -
Integrins are heterodimeric transmembrane receptors composed of an α and β subunit that mediate cell-cell and cell-extracellular matrix adhesion. As well as maintaining tissue integrity, they are involved in morphogenesis and development. (Adapted from FBrf0167428).
Protein Function (UniProtKB)
Integrin alpha-PS3/beta-PS is a receptor for laminin. Also binds to wb. Important during embryogenesis for the development of the trachea, dorsal vessel and salivary gland, as well as for dorsal closure. Required for short-term memory processes. Minor involvement in the establishment of the oocyte anterior-posterior length. Plays a role in timely border cell migration during oogenesis, probably mediated by JNK signaling. Integrin alpha-PS3/Itgbn is required for effective phagocytosis of apoptotic cells during embryonic development and for the phagocytic elimination of S.aureus by mediating the binding of S.aureus peptidoglycan to larval hemocytes, which probably activates a signaling pathway involving Rac1 and Rac2. Integrin alpha-PS3/Itgbn also regulates Fak activity during neuromuscular junction (NMJ) growth and is required for its activation in presynapsis of NMJs. Seems to be dispensable for major morphogenetic processes.
(UniProt, O44386)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
scb: scab (C. Nusslein-Volhard)
Embryonic lethal. Embryos have small mid-dorsal hole with necrotic rim.
Summary (Interactive Fly)
α-PS3 integrin - involved in dorsal closure, tracheal and salivary gland development and in the localization of pericardial cells - thought to dimerize with Myospheroid and possibly Integrin betanu subunit - Scb regulates adhesion, signalling, polarity and cell migration - Genetic interaction with Laminin genes implicates them as extracellular matrix ligands.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\scb or the JBrowse view of Dmel\scb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.50
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0087370
4304
1115
FBtr0087369
4643
1115
Additional Transcript Data and Comments
Reported size (kB)
4.6, 4.4 (northern blot)
4.9, 4.5 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0086502
124.3
1115
5.50
FBpp0086501
124.5
1115
5.86
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
1115 (aa); 90 (kD observed)
Comments
scb proteins contain a single transmembrane domain near the carboxy terminus. They differ only in the first 63 amino acids.
External Data
Subunit Structure (UniProtKB)
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Interacts with mys/beta-PS and Itgbn.
(UniProt, O44386)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\scb using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (33 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657
inferred from physical interaction with FLYBASE:Itgbn; FB:FBgn0010395
Terms Based on Predictions or Assertions (0 terms)
Biological Process (25 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (13 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from sequence or structural similarity with FLYBASE:mys; FB:FBgn0004657
traceable author statement
traceable author statement
non-traceable author statement
non-traceable author statement
traceable author statement
non-traceable author statement
traceable author statement
non-traceable author statement
traceable author statement
Cellular Component (7 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from sequence or structural similarity
non-traceable author statement
inferred from sequence or structural similarity
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
scb appears in border cells and in stretch cells at oogenesis stage 10A. It is also strongly expressed in cells that will form the dorsal appendages at stages 10B and 11 and persists until stage 13.
scb transcript was first detected in late stage 11 embryonic salivary gland after initiation of mew expression. Low levels of expression persisted in all salivary gland cells until stage 15, at which time scb was expressed at a high level.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunodetection (other than il)
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: highest levels in pupae

Additional Descriptive Data
The α3 integrin scb is generally enriched along the interface between the muscle layer and the epithelium of the midgut.
Co-localization with a hemocyte GFP marker shows that scb is present on the surface of embryonic hemocytes. A developmental western blot shows the hightest levels of scb protein in pupae and the lowest levels in larvae.
scb protein is observed starting in mid-oogenesis in dorsal appendage forming follicle cells, in stretch follicle cells, and in a posterior patch of follicle cells overlying the oocyte.
scb protein is concentrated in the mushroom body (corpora pedunculata) perikarya, calyces, peduncles and α, β, and γ lobes in adults. It is also observed in the ellipsoid body.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\scb in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Images
Alleles, Insertions, and Transgenic Constructs
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
anterior fascicle & synapse, with Scer\GAL4elav-C155
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (15)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
5 of 15
Yes
Yes
4 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
 
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
 
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (15)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
7 of 15
Yes
Yes
5 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (15)
4 of 13
Yes
Yes
3 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (5)
3 of 12
Yes
Yes
3 of 12
Yes
Yes
2 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (16)
4 of 15
Yes
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919019R )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150110 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0776 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
3 of 10
3 of 10
2 of 10
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Interacts with mys/beta-PS and Itgbn.
(UniProt, O44386 )
Linkouts
BioGRID - A database of protein and genetic interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-74
Cytogenetic map
Sequence location
2R:15,248,784..15,258,498 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
51E10-51E11
Limits computationally determined from genome sequence between P{PZ}chn02064 and P{PZ}scb01288; Left limit from non-inclusion within Df(2R)XTE-58 (FBrf0051964) Right limit from inclusion within Df(2R)l4 (FBrf0051964)
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
51E7-51E11
The l(2)51Ea complementation group maps to the genomic interval 2R:15218008..15262942 , release 6 genome (left limit from complementation mapping against Df(2R)BSC346, right limit from complementation mapping against Df(2R)Exel7135).
51E10-51E11
(determined by in situ hybridisation)
51E-51E
(determined by in situ hybridisation)
51E7-51E11
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (22)
Genomic Clones (26)
cDNA Clones (70)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Source for merge of: scb E(Sev-CycE)E93
      Source for merge of: scb l(2)51Ea
      Additional comments
      Other Comments
      S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
      scb regulates functional synaptic plasticity processes.
      mew and scb are required in a partially redundant way in the endoderm for normal migration of the endodermal midgut cells over the visceral mesoderm in the developing embryo.
      Analysis suggests the scb integrin is essential for the physiological processes underlying memory. scb integrin mediates a dynamic process.
      scb mutants display impaired olfactory memories within 3 minutes of training, indicating that the encoded α-integrin is required for short-term memory processes. Conditional expression of a scb transgene during adulthood rescues the memory impairment. Rescue is reversible, fading over time along with expression of the transgene.
      Data suggests that scb might be the gene encoding αInt3.
      The scb integrin, a cell surface protein predicted to mediate selective adhesion in response to activation by intracellular or extracellular signals, may participate in cell-cell interactions between mushroom bodies and these large interneurons underlying learning.
      The distribution of mew, if and scb proteins in cultured cell lines has been investigated.
      Origin and Etymology
      Discoverer
      Etymology
      The gene has been known as 'Volado', a Chilean colloquialism with no English counterpart, which is loosely translated as 'forgetful' or 'absent-minded'. In Chile, it is often used in reference to professors and scientists.
      Identification
      External Crossreferences and Linkouts ( 41 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Synonyms and Secondary IDs (36)
      Reported As
      Symbol Synonym
      E(Sev-CycE)E93
      E(sev-cycE)e93
      l(2)01288
      αInt3
      Secondary FlyBase IDs
      • FBgn0003328
      • FBgn0010481
      • FBgn0011707
      • FBgn0013742
      • FBgn0014404
      • FBgn0041840
      • FBgn0004158
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (228)