FB2025_01 , released February 20, 2025
Gene: Dmel\slf
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General Information
Symbol
Dmel\slf
Species
D. melanogaster
Name
schlaff
Annotation Symbol
CG3244
Feature Type
protein_coding_gene
FlyBase ID
FBgn0287234
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
schlaff (slf) encodes a chitin binding protein involved in wing formation and the substrate for the product of Tg. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Clect27

Key Links
Genomic Location
Cytogenetic map
25A6-25A6
Sequence location
Recombination map
2-13
RefSeq locus
NT_033779 REGION:4796600..4801923
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (1 term)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables carbohydrate binding
inferred from sequence or structural similarity with FLYBASE:Lectin-galC1; FB:FBgn0016675
(Tanji et al., 2006)
Biological Process (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
schlaff (slf) encodes a chitin binding protein involved in wing formation and the substrate for the product of Tg. [Date last reviewed: 2019-09-26]
Gene Group (FlyBase)
C-TYPE LECTIN-LIKE -
C-type lectins are Ca2+-dependent carbohydrate-binding proteins. They possess a C-type lectin fold, that is also present in proteins that do not bind carbohydrates. (Adapted from PMID:20301263.)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
slf: schlaff
Embryonic lethal. Cuticular differentiation normal but arrangement of cuticle around embryo abnormal. Cuticle is detached from the body. Head skeleton tilted backwards. Posterior segments contracted, anterior segments stretched around egg tip.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\slf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q8SZK9)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.45

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
slf-RA
FBtr0077417
NM_135014
1279
231
slf-RB
FBtr0332595
NM_001273117
1652
231
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
slf-PA
FBpp0077108
26.3
231
8.25
Q8SZK9
NP_608858
AAF50981
slf-PB
FBpp0304846
26.3
231
8.25
Q8SZK9
NP_001260046
AGB92582
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

231 aa isoforms: slf-PA, slf-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\slf using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.62

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 16
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic head epidermis
(Fisher et al., 2012)
embryonic ventral epidermis
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\slf in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of slf
Transgenic constructs containing regulatory region of slf
Aberrations (Deficiencies and Duplications) ( 19 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4da.G32
lethal - all die before end of P-stage | cell non-autonomous | somatic clone
lethal - all die during embryonic stage, with Scer\GAL4VP16.mat.αTub67C, Scer\GAL4da.PU
lethal - all die during embryonic stage | recessive
partially lethal - majority die, with Scer\GAL4knk.int1
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Sterility
Allele
fertile
Other Phenotypes
Allele
melanotic necrosis | adult stage, with Scer\GAL4knk.int1
melanotic necrosis | larval stage, with Scer\GAL4knk.int1
some die during embryonic stage | recessive
visible, with Scer\GAL4Bx-MS1096
visible | adult stage, with Scer\GAL4knk.int1
visible | clone of cells
visible | embryonic stage 17
visible | embryonic stage 17, with Scer\GAL4VP16.mat.αTub67C, Scer\GAL4da.PU
visible | embryonic stage 17 (with Df(2L)BSC225)
visible | embryonic stage 17 (with Df(2L)ED250)
visible | larval stage, with Scer\GAL4knk.int1
Phenotype manifest in
Allele
adult external abdomen, with Scer\GAL4knk.int1
anterior crossvein, with Scer\GAL4Bx-MS1096
cephalopharyngeal skeleton
embryo | anterior
embryo | posterior
embryonic/first instar larval cuticle
embryonic/first instar larval cuticle, with Scer\GAL4VP16.mat.αTub67C, Scer\GAL4da.PU
interommatidial bristle | clone of cells
leg joint, with Scer\GAL4knk.int1
ptilinum, with Scer\GAL4knk.int1
ptilinum | clone of cells
third instar larval cuticle, with Scer\GAL4knk.int1
wing, with Scer\GAL4Bx-MS1096
wing hinge, with Scer\GAL4knk.int1
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\ACAN
1 of 14
Yes
No
Hsap\SELL
1 of 14
Yes
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Acan
1 of 14
Yes
No
Rnor\Sell
1 of 14
Yes
No
Mus musculus (laboratory mouse) (1)
Mmus\Sell
1 of 14
Yes
No
Xenopus tropicalis (Western clawed frog) (0)
Danio rerio (Zebrafish) (36)
Drer\LOC100334522
1 of 14
Yes
Yes
Drer\LOC101883025
1 of 14
Yes
Yes
Drer\LOC103908709
1 of 14
Yes
Yes
Drer\LOC103911305
1 of 14
Yes
Yes
Drer\LOC108179228
1 of 14
Yes
Yes
Drer\LOC108190604
1 of 14
Yes
Yes
Drer\LOC110439916
1 of 14
Yes
Yes
Drer\LOC110439976
1 of 14
Yes
Yes
Drer\sele
1 of 14
Yes
No
Drer\si:cabz01083838.1
1 of 14
Yes
No
Drer\si:ch211-63p21.3
1 of 14
Yes
No
Drer\si:ch211-63p21.5
1 of 14
Yes
Yes
Drer\si:ch211-225k7.2
1 of 14
Yes
No
Drer\si:ch211-225k7.3
1 of 14
Yes
No
Drer\si:ch211-225k7.4
1 of 14
Yes
No
Drer\si:ch211-225k7.5
1 of 14
Yes
No
Drer\si:ch211-282j17.7
1 of 14
Yes
Yes
Drer\si:ch211-282j17.8
1 of 14
Yes
No
Drer\si:ch211-282j17.11
1 of 14
Yes
No
Drer\si:dkey-28d5.3
1 of 14
Yes
No
Drer\si:dkey-28d5.4
1 of 14
Yes
No
Drer\si:dkey-28d5.8
1 of 14
Yes
No
Drer\si:dkey-28d5.9
1 of 14
Yes
Yes
Drer\si:dkey-28d5.10
1 of 14
Yes
No
Drer\si:dkey-28d5.12
1 of 14
Yes
No
Drer\si:dkey-28d5.14
1 of 14
Yes
No
Drer\si:dkey-28d5.15
1 of 14
Yes
Yes
Drer\si:dkey-81n2.1
1 of 14
Yes
No
Drer\si:dkey-83f18.3
1 of 14
Yes
No
Drer\si:dkey-83f18.5
1 of 14
Yes
No
Drer\si:dkey-83f18.7
1 of 14
Yes
No
Drer\si:dkey-83f18.9
1 of 14
Yes
No
Drer\si:dkey-83f18.11
1 of 14
Yes
No
Drer\si:dkey-88n24.8
1 of 14
Yes
No
Drer\si:dkey-187j14.6
1 of 14
Yes
Yes
Drer\si:dkeyp-94g1.1
1 of 14
Yes
No
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (4)
Agam\CTLGA3
12 of 12
Yes
Yes
Agam\CTLGA1
4 of 12
No
No
Agam\CTLGA2
4 of 12
No
No
Agam\CTLMA6
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:slf. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (4)
CG4115
6 of 13
CG6055
6 of 13
Sfp24F
1 of 13
tfc
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Other Interaction Browsers
    Summary of Genetic Interactions
    esyN Network Diagram
    Other Interaction Browsers
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-13
    Cytogenetic map
    25A6-25A6
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    25A6-25A6
    Limits computationally determined from genome sequence between P{lacW}Tps1k08903&P{EP}morgueEP1184 and P{lacW}l(2)k10004k10004&P{EP}CG3036EP963
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-13

    (FlyBase, 2016)

    2-14.9

    (Comeron et al., 2012)

    2-15

    (Tearle and Nusslein-Volhard, 1987)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (8)
    Genomic Clones (12)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (87)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Zuber et al., 2019)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
          Other Comments
          Relationship to Other Genes
          Source for database merge of

          Source for merge of: slf Clect27

          (Zuber et al., 2019)
          Additional comments
          Nomenclature History
          Source for database identify of

          Source for identity of: Clect27 CG3244

          (Shibata et al., 2010)
          Nomenclature comments
          Etymology
          Synonyms and Secondary IDs (7)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 26 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          References (57)