FB2025_01 , released February 20, 2025
Gene: Dmel\IntS8
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General Information
Symbol
Dmel\IntS8
Species
D. melanogaster
Name
Integrator 8
Annotation Symbol
CG5859
Feature Type
protein_coding_gene
FlyBase ID
FBgn0289323
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Component of the Integrator complex, a complex involved in the transcription of small nuclear RNAs (snRNA) and their 3'-box-dependent processing (PubMed:21078872, PubMed:23097424). Involved in the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424). (UniProt, A1ZAK1)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

EG:EG0003.5 , l(2)SH1314

Key Links
Genomic Location
Cytogenetic map
53D13-53D13
Sequence location
Recombination map
2-80
RefSeq locus
NT_033778 REGION:16850437..16854104
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in negative regulation of BMP signaling pathway
inferred from mutant phenotype
(Liu et al., 2023)
involved_in positive regulation of gene expression
inferred from mutant phenotype
(Zhang et al., 2019)
involved_in positive regulation of neuron differentiation
inferred from mutant phenotype
(Zhang et al., 2019)
involved_in protein localization to chromatin
inferred from direct assay
(Huang et al., 2020)
involved_in snRNA 3'-end processing
inferred from direct assay
(Ezzeddine et al., 2011)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in snRNA 3'-end processing
inferred from electronic annotation with InterPro:IPR038751
(InterPro Project Members, 2004-)
involved_in snRNA processing
inferred from sequence or structural similarity with HGNC:26048
(Baillat et al., 2005)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Liu et al., 2023)
part_of integrator complex
inferred from direct assay
(Tatomer et al., 2019)
inferred from direct assay
(Lin et al., 2024)
is_active_in nucleus
inferred from direct assay
(Lin et al., 2024)
located_in nucleus
inferred from direct assay
(Liu et al., 2023)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of integrator complex
inferred from sequence or structural similarity with HGNC:26048
(Baillat et al., 2005)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the Integrator subunit 8 family. (A1ZAK1)
Protein Signatures (InterPro)
Summaries
Pathway (FlyBase)
Negative Regulators of BMP Signaling Pathway -
Negative regulators of Bone Morphogenetic Protein (BMP) signaling down-regulate the pathway, ultimately resulting in the decreased nuclear activity of the Mad/Med transcription factor complex.
Gene Group (FlyBase)
INTEGRATOR COMPLEX -
The Integrator complex is an RNA Polymerase II (Pol II)-associated endonucleolytic complex that mediates 3'-end generation of small nuclear RNAs (snRNAs) and other non-coding RNAs, and modulates Pol II promoter-proximal pausing and transcript elongation. (Adapted from PMID:36180603.)
INTEGRATOR-PP2A COMPLEX -
The Integrator-PP2A complex (INTAC) is a complex formed between the endonucleolytic integrator complex and a serine/threonine-protein phosphatase 2A (PP2A) module. The PP2A module is formed from a PP2A-A scaffold subunit and a PP2A-C catalytic subunit, but lacks the PP2A-B regulatory subunit characteristic of canonical PP2A complexes. INTAC inhibits promoter-proximal pause release of RNA polymerase II (Pol II) by the dephosphorylation of a number of targets including the C-terminal domain of Pol II. (Adapted from PMID:36180603.)
Protein Function (UniProtKB)
Component of the Integrator complex, a complex involved in the transcription of small nuclear RNAs (snRNA) and their 3'-box-dependent processing (PubMed:21078872, PubMed:23097424). Involved in the 3'-end processing of the U7 snRNA, and also the spliceosomal snRNAs U1, U2, U4 and U5 (PubMed:21078872, PubMed:23097424).
(UniProt, A1ZAK1)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\IntS8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A1ZAK1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
IntS8-RA
FBtr0087036
NM_137318
3293
1007
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
IntS8-PA
FBpp0086186
113.5
1007
6.47
A1ZAK1
NP_611162
AAF57945
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Belongs to the multiprotein complex Integrator, at least composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7, IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.

(UniProt, A1ZAK1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\IntS8 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.66

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Liu et al., 2023)
part_of integrator complex
inferred from direct assay
(Tatomer et al., 2019)
inferred from direct assay
(Lin et al., 2024)
is_active_in nucleus
inferred from direct assay
(Lin et al., 2024)
located_in nucleus
inferred from direct assay
(Liu et al., 2023)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\IntS8 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of IntS8
Transgenic constructs containing regulatory region of IntS8
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(2R)BSC382
(Kahsai et al., 2016)
Df(2R)mir-8Δ3
(Karres et al., 2007)
Inferred from location ( 6 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\INTS8
14 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Ints8
11 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Ints8
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\ints8
7 of 13
Yes
Yes
Xtro\prkaca
1 of 13
No
No
Danio rerio (Zebrafish) (1)
Drer\ints8
14 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\ints-8
4 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP011449
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:IntS8. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
INTS8; integrator complex subunit 8
14 of 14
611351
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Other Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    IntS8 - Dad
    anti tag coimmunoprecipitation, anti tag western blot
    (Liu et al., 2023)
    IntS8 - IntS1
    anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry
    (Lin et al., 2024, Huang et al., 2020)
    IntS8 - IntS11
    anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry, pull down
    (Lin et al., 2024, Lin et al., 2022, Huang et al., 2020)
    IntS8 - IntS12
    anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry
    (Lin et al., 2024, Huang et al., 2020)
    IntS8 - IntS4
    anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry
    (Lin et al., 2024, Huang et al., 2020)
    IntS8 - IntS9
    anti tag coimmunoprecipitation, western blot, pull down, Identification by mass spectrometry
    (Lin et al., 2024, Lin et al., 2022, Huang et al., 2020)
    IntS8 - Pp2A-29B
    anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot, two hybrid
    (Huang et al., 2020)
    IntS8 - Smurf
    anti tag coimmunoprecipitation, anti tag western blot
    (Liu et al., 2023)
    IntS8 - defl
    anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot
    (Huang et al., 2020)
    IntS8 - mts
    anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry
    (Huang et al., 2020)
    IntS8 - omd
    two hybrid, anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry
    (Lin et al., 2024, Huang et al., 2020)
    IntS8 - tkv
    anti tag coimmunoprecipitation, western blot, anti tag western blot
    (Liu et al., 2023)
    Summary of Genetic Interactions
    esyN Network Diagram
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    esyN Network Key:
    Suppression
    Enhancement
    Other Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    IntS8
    suppressible
    erm
    (Zhang et al., 2019)
    IntS8
    enhanceable
    erm
    (Zhang et al., 2019)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Ppt1
    suppressible
    IntS8
    (Buff et al., 2007)
    External Data
    Subunit Structure (UniProtKB)
    Belongs to the multiprotein complex Integrator, at least composed of IntS1, IntS2, IntS3, IntS4, omd/IntS5, IntS6, defl/IntS7, IntS8, IntS9, IntS10, IntS11, IntS12, asun/IntS13 and IntS14.
    (UniProt, A1ZAK1 )
    Linkouts
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-80
    Cytogenetic map
    53D13-53D13
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    53D13-53D13
    Limits computationally determined from genome sequence between P{EP}BEST:SD02913EP2148&P{lacW}Dekk09907 and P{lacW}CG6426k10209&P{lacW}vegk07202
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    53D14-53D14
    (Oh et al., 2003)
    Experimentally Determined Recombination Data
    Location

    2-80

    (FlyBase, 2016)

    2-84.5

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (13)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (16)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Fujiwara et al., 2023, Liu et al., 2023, Huang et al., 2020)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
        Other Comments

        1 allele of l(2)SH1314 recovered in a P-insertion screen.

        (Oh et al., 2003)
        Relationship to Other Genes
        Source for database merge of

        Source for merge of: IntS8 l(2)SH1314

        (Liu et al., 2023)
        Additional comments
        Nomenclature History
        Source for database identify of

        Source for identity of: IntS8 CG5859

        (Baillat et al., 2005)
        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (10)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 24 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        References (53)