FlyBase Gene Report: Dmel\blo

General Information

Symbol

Dmel\blo

Species

D. melanogaster

Name

bloated

Annotation Symbol

CG8740

Feature Type

protein_coding_gene

FlyBase ID

FBgn0289556

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Red Book
Interactive Fly

Also Known As

kitikete, BcDNA:GH05582 , kit

Key Links

Genomic Location

Cytogenetic map

44E3-44E4

Sequence location

2R:8,735,810..8,759,590 [+]

Recombination map

2-59

RefSeq locus

NT_033778 REGION:8735810..8759590

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in apposition of dorsal and ventral imaginal disc-derived wing surfaces

inferred from mutant phenotype

(Walsh and Brown, 1998)

involved_in cell-cell adhesion

inferred from mutant phenotype

(Walsh and Brown, 1998)

involved_in imaginal disc-derived wing morphogenesis

inferred from mutant phenotype

(Walsh and Brown, 1998)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

blo: bloated

Wings spread, crumpled, and vesiculated; wing shows irregular plexus of extra veins. In extreme cases, wings unexpanded. Occasional hooked or wavy bristles. Developmental studies by Waddington [1939, Proc. Nat. Acad. Sci. USA 25: 299-307 and 1940, J. Genet. 41: 75-139 (fig.)] show intervein material spongy and veins swollen with inadequate contraction after inflated stage of pupal wing. Droplets of hemolymph often become clothed with cells liberated from epithelium and remain along basal processes. Does not overlap wild type but has poor viability and hatches later. RK2.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\blo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry B7YZT7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

blo-RA

FBtr0088713

NM_136567

4136

985

blo-RB

FBtr0088711

NM_165626

4593

985

blo-RC

FBtr0088712

NM_165627

4608

985

blo-RD

FBtr0290115

NM_001144149

4918

1269

blo-RE

FBtr0339338

NM_001299283

4882

1257

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

blo-PA

FBpp0087792

112.9

985

10.84

FBpp0087790

112.9

985

10.84

FBpp0087791

112.9

985

10.84

FBpp0288554

144.3

1269

10.82

FBpp0308438

143.0

1257

10.87

A0A0B4LEW1

NP_001286212

AHN56010

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

985 aa isoforms: blo-PA, blo-PB, blo-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\blo using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.74

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\blo in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\blo

View exonic expression by tissue for Dmel\blo

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35866/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0289556

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0289556

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of blo

Transgenic constructs containing regulatory region of blo

Aberrations (Deficiencies and Duplications) ( 14 )

Inferred from experimentation ( 14 )

Gene disrupted in

Df(2R)blo^Δ10

(Brown, 2023.8.29)

Df(2R)BSC268

(Kahsai and Cook, 2018)

Df(2R)BSC269

(Kahsai and Cook, 2018)

Df(2R)ED1770

(Kahsai and Cook, 2018)

Df(2R)Np3

(Walsh and Brown, 1998, Prout et al., 1997)

In(2LR)pkD1-rv11

(Roote, 1997.6.6)

Gene not disrupted in

Df(2R)BSC270

(Kahsai and Cook, 2018)

Df(2R)BSC271

(Kahsai and Cook, 2018)

Df(2R)ED1742

(Kahsai and Cook, 2018)

Df(2R)ED1791

(Kahsai and Cook, 2018)

Df(2R)en-A

(Chase and Baker, 1995)

Df(2R)en-B

(Chase and Baker, 1995)

Df(2R)Exel7098

(Kahsai and Cook, 2018)

Df(2R)Np

(Bridges et al., 1936)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

blo^GD14014

lethal - all die before end of larval stage | recessive

partially lethal

blo¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

blo^GD14014

Other Phenotypes

Allele

abnormal planar polarity, with Scer\GAL4^pnr-MD237

blo^GD14014

some die during larval stage | recessive

visible, with Scer\GAL4^pnr-MD237

blo^GD14014

visible (with blo¹)

visible (with blo^V99)

blo¹

visible (with blo^V134)

blo¹

visible | recessive

blo¹

visible | somatic clone

Phenotype manifest in

Allele

macrochaeta

blo¹

trichogen cell, with Scer\GAL4^pnr-MD237

blo^GD14014

wing

blo¹

wing (with blo¹)

wing (with blo^V99)

blo¹

wing (with blo^V134)

blo¹

wing | somatic clone

wing vein

blo¹

wing vein | cell non-autonomous | ectopic | somatic clone

blo^V99

wing vein | ectopic

blo^V99

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP012181

9 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:blo. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

blo

(Walsh and Brown, 1998)

mew

enhanceable

blo

(Walsh and Brown, 1998)

mys

enhanceable

blo

(Prout et al., 1997)

External Data

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-59

Cytogenetic map

44E3-44E4

Sequence location

2R:8,735,810..8,759,590 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

44E3-44E4

Limits computationally determined from genome sequence between P{PZ}l(2)44DEa⁰⁵⁸⁴⁷ and P{lacW}Rya-r44F^k04913

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Not included within, but does not recombine with (0/1098), Df(2R)Np.

(Bridges et al., 1936)

The blo complementation group maps to the genomic interval 2R:8733630..8898753 , release 6 genome (left limit from complementation mapping against Df(2R)Exel7098, right limit from complementation mapping against Df(2R)BSC269).

(Kahsai and Cook, 2018)

Experimentally Determined Recombination Data

Location

2-59

(FlyBase, 2016)

2-57.8

(Comeron et al., 2012)

(Prout et al., 1997)

2-58.5

Left of (cM)

Right of (cM)

(Plough and Ives, 1934)

Notes

Located on 2R.

(Prout et al., 1997)

Stocks and Reagents

Stocks (15)

Genomic Clones (39)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (49)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH05582

Other clones

FI03652

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones. 3 alleles have been isolated.

(Prout et al., 1997)

Relationship to Other Genes

Source for database merge of

Source for merge of: blo kit

(Walsh and Brown, 1998)

Source for merge of: blo CG8740

(Brown, 2023.8.29)

Additional comments

The blo complementation group was identified as corresponding to CG8740 partly from the noncomplementation of Df(2R)blo[Δ10] with preexisting blo mutant alleles.

(Brown, 2023.8.29)

The blo complementation group corresponds to one (or more) of the following annotated genes: CG8740, CG42615, CR44277, CR44278, CG8746, CG30350, CG8272, DCTN2-p50, Sec31, CR45202, ana2.

(Kahsai and Cook, 2018)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

BcDNA:GH05582

(Giot et al., 2003)

CG8740

(Zirin, 2024-, Brown, 2023.8.29, National Institute of Genetics Fly Stocks, 2022-, Pang et al., 2021, Clandinin and Owens, 2016-, Hosono et al., 2015, Sen et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Wasbrough et al., 2010, Keleman et al., 2009.8.5, Molnar et al., 2006, Raymond et al., 2004)

(Plough and Ives, 1934)

ba²

ba^33f26

blo

(Brown, 2023.8.29, Kahsai and Cook, 2018, Carmon et al., 2010)

kit

(Prout et al., 1997)

Name Synonyms

balloon

bloated

(Carmon et al., 2010)

kitikete

(Carmon et al., 2010, MacIntyre et al., 2007, Prout et al., 1997)

Secondary FlyBase IDs

FBgn0027585
FBgn0020848
FBgn0000198

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 34 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35866

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

B7YZT7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

B7YZT7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35866/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0289556

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0289556

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8740

MARRVEL_MODEL - MARRVEL (model organism gene)

35866

Linkouts

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0289556

References (54)

The following external sites may use different assemblies or annotations than FlyBase.

-0.74

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