FlyBase Gene Report: Dmel\CG4199

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG4199 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.73

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fat body

Comment: cyclical, peak ZT13-16

(Xu et al., 2011)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

embryonic stage 7 -- 8

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

embryonic leading edge cell primordium

Comment: reported as embryonic leading edge cell specific anlage

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic leading edge cell

(Fisher et al., 2012)

embryonic proventriculus

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

Additional Descriptive Data

Expressed cyclically in the adult fat body.

(Xu et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010, Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG4199 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG4199

View exonic expression by tissue for Dmel\CG4199

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31174/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0289578

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0289578

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG4199

Transgenic constructs containing regulatory region of CG4199

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel6289

(Ryder, 2004.4.26)

Inferred from location ( 25 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

CG4199^e04396

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG4199^GD11247

viable, with Scer\GAL4^Mef2.PR

CG4199^GD11247

viable, with Scer\GAL4^pnr-MD237

CG4199^GD11247

viable, with Scer\GAL4^tin.CΔ4

CG4199^GD11247

Sterility

Allele

fertile

CG4199^e04396

Other Phenotypes

Allele

decreased rate of movement | adult stage | male limited, with Scer\GAL4^elav-C155

CG4199^VDRC.cUa

increased sleep | female limited, with Scer\GAL4^elav-C155

CG4199^VDRC.cUa

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (11)

Hsap\AIFM3

12 of 14

Yes

Hsap\AIFM1

2 of 14

Hsap\AIFM2

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (9)

Rnor\Aifm3

12 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Sqor

1 of 14

Rnor\Txnrd1

1 of 14

Rnor\Txnrd2

1 of 14

Mus musculus (laboratory mouse) (10)

Mmus\Aifm3

11 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Sqor

1 of 14

Mmus\Txnrd1

1 of 14

Mmus\Txnrd2

1 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\LOC100498581

11 of 13

Yes

Xtro\aifm3

6 of 13

Yes

Xtro\aifm1

1 of 13

Xtro\LOC100495217

1 of 13

Yes

Xtro\XB5959486

1 of 13

Yes

Danio rerio (Zebrafish) (13)

Drer\aifm5

13 of 14

Yes

Drer\aifm3

12 of 14

Yes

Drer\aifm4

11 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\pyroxd1

1 of 14

Drer\rfesd

1 of 14

Yes

Drer\si:ch73-314g15.3

1 of 14

Yes

Drer\si:ch211-212d10.2

1 of 14

Yes

Drer\sqor

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\F20D6.11

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP003904

10 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\MDAR4

11 of 13

Yes

Atha\ATMDAR2

10 of 13

Yes

Atha\MDAR1

10 of 13

Yes

Atha\MDAR6

10 of 13

Yes

Atha\MDHAR

10 of 13

Yes

Atha\AT1G71500

1 of 13

Yes

Atha\AT3G44190

1 of 13

Yes

Atha\AT4G16155

1 of 13

Atha\AT5G22140

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\NDB2

1 of 13

Yes

Atha\NDB3

1 of 13

Yes

Atha\NDC1

1 of 13

Atha\NTRA

1 of 13

Yes

Atha\NTRB

1 of 13

Yes

Atha\NTRC

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\AIF1

2 of 13

Yes

Scer\TRR1

2 of 13

Yes

Scer\TRR2

2 of 13

Yes

Scer\GLR1

1 of 13

Scer\IRC15

1 of 13

Scer\LPD1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\aif1

11 of 12

Yes

Spom\dld1

1 of 12

Spom\hmt2

1 of 12

Spom\SPBC947.15c

1 of 12

Yes

Escherichia coli (enterobacterium) (10)

Ecol\hcaD

9 of 11

Yes

Ecol\norW

4 of 11

Yes

Ecol\hcaC

2 of 11

Yes

Ecol\ahpF

1 of 11

Yes

Ecol\fadH

1 of 11

Yes

Ecol\gor

1 of 11

Ecol\lpd

1 of 11

Ecol\ndh

1 of 11

Yes

Ecol\nirB

1 of 11

Ecol\sthA

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG4199. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

CG10700

13 of 13

AIF

4 of 13

Pyroxd1

3 of 13

Dld

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

AIFM3; AIF family member 3

12 of 14

617298

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG4199 - Cisd

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-0.5

Cytogenetic map

2C9-2C10

Sequence location

X:2,066,435..2,073,483 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

2C9-2C10

Limits computationally determined from genome sequence between P{EP}Actn^EP1193&P{EP}CG4322^EP1631 and P{EP}EP1460&P{EP}CG2924^EP1596

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-0.5

(FlyBase, 2016)

1-0.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

33468

P{EP}CG4199^G1084 w^*

21868

P{EPg}Rtca^HP10306 CG4199^HP10306 w¹¹¹⁸

85871

y¹ sc^* v¹ sev²¹; P{TKO.GS04236}attP40

10749

4199R-2

v106170

v26424

w¹¹¹⁸; P{GD11247}v26424

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (67)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD06560

Other clones

DPiM_SD06560

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Dia1 and Dia2 have been isolated from D.melanogaster and D.virilis, purified and their biochemical and kinetic properties characterized. Antibodies were raised to the proteins from D.virilis to study the relations of Dia1 and Dia2 to similar proteins in D.novamexicana and D.melanogaster.

(Ralchev et al., 1992)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG4199 Dia1

(Ivanova et al., 2008)

Additional comments

The CG10700 gene may have been derived from the CG4199 gene by retroposition.

(Dai et al., 2006)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

143005_at

(Kristensen et al., 2005)

DIA-1

(Ivanova et al., 2008, Choudhary et al., 1992)

Dia1

DmelI

(Ivanova et al., 2008)

EG:22E5.5

(Stuart et al., 2007, Giot et al., 2003, Benos et al., 2001)

Name Synonyms

Diaphorase 1

diaphorase-1

(Ivanova et al., 2008, Ralchev et al., 1992)

Secondary FlyBase IDs

FBgn0025628
FBgn0005397

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31174

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q960D4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q960D4

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31174/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0289578

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0289578

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4199

MARRVEL_MODEL - MARRVEL (model organism gene)

31174

Linkouts

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0289578

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006048

References (66)

The following external sites may use different assemblies or annotations than FlyBase.

1.73

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