FlyBase Gene Report: Dmel\Nsf2

General Information

Symbol

Dmel\Nsf2

Species

D. melanogaster

Name

N-ethylmaleimide-sensitive factor 2

Annotation Symbol

CG33101

Feature Type

protein_coding_gene

FlyBase ID

FBgn0290174

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

N-ethylmaleimide-sensitive factor 2 (Nsf2) encodes a homohexameric AAA ATPase that functions to recycle SNARE complex components subsequent to membrane fusion. In the tracheal system, it is required for tube growth and connectivity within the terminal cell. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

dNSF2, dNSF-2, urd, NEM-sensitive fusion protein 2, lotus

Key Links

Genomic Location

Cytogenetic map

87F15-87F15

Sequence location

3R:13,833,497..13,837,991 [-]

Recombination map

3-54

RefSeq locus

NT_033777 REGION:13833497..13837991

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960, InterPro:IPR039812

(InterPro Project Members, 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in SNARE complex disassembly

inferred from electronic annotation with InterPro:IPR039812

(InterPro Project Members, 2004-)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

N-ETHYLMALEIMIDE-SENSITIVE FACTORS

Protein Family (UniProt)

Belongs to the AAA ATPase family. (P54351)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Snapshot

Gene Group (FlyBase)

N-ETHYLMALEIMIDE-SENSITIVE FACTORS -: The N-ethylmaleimide-sensitive factor/fusion protein (NSF), is a hexameric ATPase recruited to SNARE-SNAP complexes. ATP hydrolysis by NSF, leads to conformational changes and dissociation of the SNARE complex. (Adapted from FBrf0141270).

Protein Function (UniProtKB)

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin (By similarity).

(UniProt, P54351)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

urd: urdur (J.A. Kennison)

Isolated as a dominant suppressor of Pc mutations. Also suppresses Pcl and Msc alleles. Recessive larval lethal.

Summary (Interactive Fly)

homohexameric AAA ATPase - developmentally regulated N-ethylmaleimide-sensitive fusion (NSF) gene - mutants are defective in the synaptic growth and maturation that occurs during larval development - functions to recycle SNARE complex components subsequent to membrane fusion - required for tracheal tube growth and connectivity within the terminal cell

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nsf2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P54351)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nsf2-RA

FBtr0082883

NM_176499

2427

752

Nsf2-RB

FBtr0344766

NM_001300389

2823

752

Nsf2-RC

FBtr0344767

NM_001300390

3521

752

Additional Transcript Data and Comments

Reported size (kB)

2.4 (northern blot)

(Boulianne and Trimble, 1995)

3.4, 3.0, 2.8, 2.4 (northern blot)

(Pallanck et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Nsf2-PA

FBpp0082346

83.4

752

6.71

FBpp0311094

83.4

752

6.71

FBpp0311095

83.4

752

6.71

P54351

NP_001287319

AHN57318

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

752 aa isoforms: Nsf2-PA, Nsf2-PB, Nsf2-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

744 (aa)

(Boulianne and Trimble, 1995)

752 (aa)

(Pallanck et al., 1995)

Comments

External Data

Subunit Structure (UniProtKB)

Homohexamer.

(UniProt, P54351)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nsf2 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.73

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval midgut

(Boulianne and Trimble, 1995)

embryonic/larval hindgut

(Boulianne and Trimble, 1995)

embryonic stage 5 -- 6

organism | ubiquitous

(Boulianne and Trimble, 1995)

embryonic stage 8 -- 10

posterior midgut primordium

(Boulianne and Trimble, 1995)

anterior midgut primordium

(Boulianne and Trimble, 1995)

embryonic stage 8 -- 11

mesoderm

(Boulianne and Trimble, 1995)

ectoderm

(Boulianne and Trimble, 1995)

embryonic stage 14 -- 17

presumptive embryonic/larval central nervous system

(Boulianne and Trimble, 1995)

larval stage

embryonic/larval salivary gland | restricted

(Boulianne and Trimble, 1995)

eye-antennal disc

(Boulianne and Trimble, 1995)

ring gland

(Boulianne and Trimble, 1995)

wing disc

(Boulianne and Trimble, 1995)

leg disc

(Boulianne and Trimble, 1995)

imaginal disc

(Boulianne and Trimble, 1995)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage & adult stage

(Pallanck et al., 1995)

Additional Descriptive Data

The highest levels of Nsf2 transcripts are seen before and during cellularization. After gastrulation, expression is seen primarily in regions which give rise to ectodermal and endodermal tissues such as the midgut and the hindgut. Later, expression is also seen in the CNS. In larvae, Nsf2 expression is observed in the ring gland and in a restricted number of cells in the salivary gland. Expression is also observed in unusual patterns in the imaginal discs with the highest levels in the leg, wing and antennal discs.

(Boulianne and Trimble, 1995)

Nsf2 transcripts are expressed at substantially higher levels in adults and larvae than in embryos.

(Pallanck et al., 1995)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nsf2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nsf2

View exonic expression by tissue for Dmel\Nsf2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41694/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0290174

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0290174

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 19 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nsf2

Transgenic constructs containing regulatory region of Nsf2

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3R)BSC611

(Cook et al., 2008.9.3)

Df(3R)red3l

(Netter et al., 1998)

Df(3R)urd

(Golby et al., 2001)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(3R)BSC611)

Nsf2^urd-2

lethal (with Nsf2^A6)

Nsf2^urd-2

lethal (with Nsf2^A15)

Nsf2^urd-2

lethal (with Nsf2^urd-2)

lethal | germline clone | larval stage | recessive

Nsf2⁵⁵

lethal | larval stage | recessive

Nsf2⁵⁵

lethal | recessive

lethal - all die before end of larval stage

lethal - all die before end of larval stage | recessive

lethal - all die before end of P-stage | recessive

Nsf2⁴²

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Nsf2^GD1327

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Nsf2^GD1327

viable

Nsf2^A6

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Nsf2^GD1327

viable, with Scer\GAL4^tin.CΔ4

Nsf2^GD1327

Other Phenotypes

Allele

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

Nsf2^EQ.UAS

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav.PLu

Nsf2^EQ.UAS

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

abnormal neurophysiology | larval stage, with Scer\GAL4^elav.PLu

Nsf2^EQ.UAS

abnormal neurophysiology | larval stage, with Scer\GAL4^elav-C155

Nsf2^EQ.UAS

some die during larval stage

some die during larval stage | recessive

some die during pharate adult stage | recessive

Nsf2⁴²

some die during pupal stage, with Scer\GAL4^pnr-MD237

Nsf2^GD1327

visible

Nsf2^A6

visible, with Scer\GAL4^bbg-C96

Nsf2^EQ.UAS

visible, with Scer\GAL4^pnr-MD237

Nsf2^GD1327

visible | adult stage, with Scer\GAL4^bbg-C96

Nsf2^EQ.UAS

wild-type

Nsf2^hs.PG

wild-type, with Scer\GAL4^bbg-C96

Nsf2^WT.UAS

Phenotype manifest in

Allele

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Nsf2^JF02765

embryonic/larval neuromuscular junction, with Scer\GAL4^BG487

Nsf2^EQ.UAS

embryonic/larval neuromuscular junction, with Scer\GAL4^elav.PLu

Nsf2^EQ.UAS

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Nsf2^JF02765

eye

Nsf2^A6

larval tracheal system

Nsf2^lotus-312

mesonotum, with Scer\GAL4^pnr-MD237

Nsf2^GD1327

synaptic vesicle, with Scer\GAL4^elav-C155

Nsf2^EQ.UAS

terminal tracheal cell

Nsf2^lotus-312

terminal tracheal cell | somatic clone | third instar larval stage

Nsf2^lotus-312

terminal tracheal cell | third instar larval stage

terminal tracheal cell | third instar larval stage, with Scer\GAL4^bs.PM

Nsf2^GD1327

trichogen cell, with Scer\GAL4^pnr-MD237

Nsf2^GD1327

wing margin, with Scer\GAL4^bbg-C96

Nsf2^EQ.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (24)

Hsap\NSF

14 of 14

Yes

Hsap\ATAD1

2 of 14

Hsap\ATAD2B

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Nsf

13 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Nsf

13 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (20)

Xtro\nsf

7 of 13

Yes

Xtro\atad2b

1 of 13

Yes

Xtro\atad2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\pex1

1 of 13

Xtro\pex6

1 of 13

Xtro\prxl2a

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (18)

Drer\nsfa

14 of 14

Yes

Drer\nsfb

13 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:dkey-195m11.8

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (19)

Cele\nsf-1

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (25)

Agam\AgaP_AGAP012684

11 of 12

Yes

Agam\AgaP_AGAP012537

4 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (48)

Atha\NSF

11 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (16)

Scer\SEC18

13 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\sec18

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Nsf2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (24)

12 of 13

6 of 13

2 of 13

2 of 13

nmd

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

bor

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Nsf2^EQ.UAS

model of Parkinson's disease

CEA

(Stewart et al., 2002)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

NSF; N-ethylmaleimide sensitive factor, vesicle fusing ATPase

model of developmental and epileptic encephalopathy 96

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Nsf2^HMS01262

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Nsf2^urd-2

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NSF; N-ethylmaleimide sensitive factor, vesicle fusing ATPase

14 of 14

N-ETHYLMALEIMIDE-SENSITIVE FACTOR; NSF

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 96; DEE96

developmental and epileptic encephalopathy 96

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Nsf2 - Bet1

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Membrin

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Sec20

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Sec22

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Slh

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Snap25

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Snap29

anti bait coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Morelli et al., 2014, Guruharsha et al., 2011)

Nsf2 - Syx18

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Syx1A

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Syx5

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Syx8

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Use1

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Vamp7

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - Ykt6

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - comt

two hybrid

(Hu et al., 2017.6.13)

Nsf2 - αSnap

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - γSnap1

experimental knowledge based

(Guruharsha et al., 2011)

Nsf2 - γSnap2

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nsf2

suppressible

Act5C

(Laviolette et al., 2005)

Nsf2

suppressible

Bacc

(Laviolette et al., 2005)

Nsf2

suppressible

Baldspot

(Laviolette et al., 2005)

Nsf2

suppressible

ben

(Laviolette et al., 2005)

Nsf2

enhanceable

bib

(Stewart et al., 2001)

Nsf2

suppressible

brat

(Laviolette et al., 2005)

Nsf2

suppressible

btn

(Laviolette et al., 2005)

Nsf2

suppressible

C3G

(Laviolette et al., 2005)

Nsf2

suppressible

CCT7

(Laviolette et al., 2005)

Nsf2

suppressible

Cds

(Laviolette et al., 2005)

Nsf2

suppressible

CG1806

(Laviolette et al., 2005)

Nsf2

suppressible

CG1888

(Laviolette et al., 2005)

Nsf2

suppressible

CG3603

(Laviolette et al., 2005)

Nsf2

suppressible

CG4119

(Laviolette et al., 2005)

Nsf2

suppressible

CG7492

(Laviolette et al., 2005)

Nsf2

suppressible

CG10801

(Laviolette et al., 2005)

Nsf2

suppressible

CG14411

(Laviolette et al., 2005)

Nsf2

suppressible

CG14628

(Laviolette et al., 2005)

Nsf2

suppressible

CG32650

(Laviolette et al., 2005)

Nsf2

suppressible

CG42450

(Laviolette et al., 2005)

Nsf2

suppressible

CG45263

(Laviolette et al., 2005)

Nsf2

suppressible

comt

(Golby et al., 2001)

Nsf2

suppressible

cpo

(Laviolette et al., 2005)

Nsf2

suppressible

Cyp6v1

(Laviolette et al., 2005)

Nsf2

suppressible

Delta

(Stewart et al., 2001)

Nsf2

enhanceable

Delta

(Stewart et al., 2001)

Nsf2

suppressible

E2f1

(Laviolette et al., 2005)

Nsf2

suppressible

fend

(Laviolette et al., 2005)

Nsf2

suppressible

Fim

(Laviolette et al., 2005)

Nsf2

enhanceable

fng

(Stewart et al., 2001)

Nsf2

suppressible

fz4

(Laviolette et al., 2005)

Nsf2

suppressible

Gdi

(Laviolette et al., 2005)

Nsf2

suppressible

Gli

(Laviolette et al., 2005)

Nsf2

suppressible

grp

(Laviolette et al., 2005)

Nsf2

suppressible

GS32

(Laviolette et al., 2005)

Nsf2

suppressible

GS1027

(Laviolette et al., 2005)

Nsf2

suppressible

GS2192

(Laviolette et al., 2005)

Nsf2

suppressible

GS2285

(Laviolette et al., 2005)

Nsf2

suppressible

GS3087

(Laviolette et al., 2005)

Nsf2

suppressible

GS5036

(Laviolette et al., 2005)

Nsf2

suppressible

GS5071

(Laviolette et al., 2005)

Nsf2

suppressible

GS5246

(Laviolette et al., 2005)

Nsf2

suppressible

GS8172

(Laviolette et al., 2005)

Nsf2

suppressible

GS14505

(Laviolette et al., 2005)

Nsf2

suppressible

igl

(Laviolette et al., 2005)

Nsf2

suppressible

kuz

(Laviolette et al., 2005)

Nsf2

suppressible

LanA

(Laviolette et al., 2005)

Nsf2

suppressible

lola

(Laviolette et al., 2005)

Nsf2

suppressible

Lon

(Laviolette et al., 2005)

Nsf2

suppressible

Moe

(Laviolette et al., 2005)

Nsf2

suppressible

Mppe

(Laviolette et al., 2005)

Nsf2

suppressible

(Stewart et al., 2001)

Nsf2

suppressible

nej

(Laviolette et al., 2005)

Nsf2

suppressible

NetB

(Laviolette et al., 2005)

Nsf2

suppressible

ovo

(Laviolette et al., 2005)

Nsf2

suppressible

Pdss2

(Laviolette et al., 2005)

Nsf2

suppressible

phtf

(Laviolette et al., 2005)

Nsf2

suppressible

polo

(Laviolette et al., 2005)

Nsf2

suppressible

Polr1D

(Laviolette et al., 2005)

Nsf2

enhanceable

por

(Stewart et al., 2001)

Nsf2

suppressible

Qtzl

(Laviolette et al., 2005)

Nsf2

suppressible

Rab5

(Laviolette et al., 2005)

Nsf2

suppressible

Rbf

(Laviolette et al., 2005)

Nsf2

suppressible

rdog

(Laviolette et al., 2005)

Nsf2

suppressible

RhoBTB

(Laviolette et al., 2005)

Nsf2

suppressible

RpS2

(Laviolette et al., 2005)

Nsf2

suppressible

sac

(Laviolette et al., 2005)

Nsf2

enhanceable

Ser

(Stewart et al., 2001)

Nsf2

suppressible

Ser

(Stewart et al., 2001)

Nsf2

suppressible

spi

(Laviolette et al., 2005)

Nsf2

enhanceable

Syb

(Stewart et al., 2001)

Nsf2

suppressible

Syn2

(Laviolette et al., 2005)

Nsf2

enhanceable

Syx1A

(Stewart et al., 2001)

enhanceable

(Xu et al., 2002)

enhanceable

(Xu et al., 2002)

suppressible

(Laviolette et al., 2005)

Nsf2

suppressible

Ubi-p5E

(Laviolette et al., 2005)

Nsf2

suppressible

ubl

(Laviolette et al., 2005)

Nsf2

suppressible

Ufd1

(Laviolette et al., 2005)

Nsf2

suppressible

wech

(Laviolette et al., 2005)

Nsf2

enhanceable

(Stewart et al., 2001)

Nsf2

suppressible

wuho

(Laviolette et al., 2005)

Nsf2

enhanceable

Yif1

(Kandasamy et al., 2021)

Nsf2

suppressible

αSnap

(Laviolette et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Antp|Pc

suppressible

Nsf2

(Kennison and Tamkun, 1988)

cnk

enhanceable

Nsf2

(Baril et al., 2014)

comt

suppressible

Nsf2

(Golby et al., 2001)

CycE

suppressible

Nsf2

(Brumby et al., 2002)

E(z)

suppressible

Nsf2

(Bajusz et al., 2001)

E(z)

enhanceable

Nsf2

(Bajusz et al., 2001)

GckIII

suppressible

Nsf2

(Song et al., 2013)

suppressible

Nsf2

(Kennison and Tamkun, 1988)

αSnap

suppressible

Nsf2

(Babcock et al., 2004)

αSnap

enhanceable

Nsf2

(Babcock et al., 2004)

External Data

Subunit Structure (UniProtKB)

Homohexamer.

(UniProt, P54351 )

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

(Ghabrial et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-54

Cytogenetic map

87F15-87F15

Sequence location

3R:13,833,497..13,837,991 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87F15-87F15

Limits computationally determined from genome sequence between P{PZ}flfl⁰¹⁹⁴⁹ and P{lacW}trx^j14A6&P{PZ}trx⁰⁰³⁴⁷; Left limit from inclusion within Df(3R)red3l (FBrf0102846) Right limit from inclusion within Df(3R)urd (citation unavailable)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.

(Lloyd et al., 2000)

87F-87F

(Lloyd et al., 2000)

87F12-88A3

(Trimble, 1995.6.8)

87F12-87F15

(Kennison, 1993)

Experimentally Determined Recombination Data

Location

3-54

(FlyBase, 2016)

3-51.3

(Comeron et al., 2012)

(Ghabrial et al., 2011, Trimble, 1995.6.8)

3-53

(Kennison and Tamkun, 1988)

Left of (cM)

Right of (cM)

Notes

Maps to 3R.

(Ghabrial et al., 2011)

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

5049

Nsf2^urd-2/TM3, Sb¹

81904

w^*; PBac{U6:3-Nsf2.gRNA}VK00027

27685

y¹ v¹; P{TRiP.JF02765}attP2

8282

w¹¹¹⁸; Nsf2^A15/TM6B, Tb¹

81909

w^*; PBac{U6:3-comt-Nsf2.gRNA.GGGG(FE)}VK00027

8281

w¹¹¹⁸; Nsf2^A6/TM3, Sb¹ Ser¹

82047

y¹ sc^* v¹ sev²¹; P{TKO.GS04488}attP40

606442

w^*; PBac{U6:3-comt-Nsf2.gRNA.QQQ(2.1)}VK00027/TM6B, Tb¹

34914

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01262}attP2

Kyoto

108009

Nsf2^urd-2/TM6C, cu¹ Sb¹ ca¹

VDRC

v330318

P{VSH330318}attP40

v7743

w¹¹¹⁸; P{GD1327}v7743

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (153)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD09622

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

ChEST reveals this is a target of Mef2.

(Junion et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per⁰¹, tim⁰¹ and increased expression in Clk^Jrk background.

(Claridge-Chang et al., 2001)

comt and Nsf2 exhibit similar functional properties but have evolved distinct tissue-specific roles. comt is required in the nervous system beginning at the adult stage of development whereas Nsf2 is required in the mesoderm beginning at the first larval instar. The comt and Nsf2 proteins can functionally substitute for one another.

(Golby et al., 2001)

Evidence of multiple, highly related isoforms of NSF in a single species suggests that in some tissue membranes fusion and/or secretion has a specific role during development.

(Boulianne and Trimble, 1995)

Sections of the Scr regulatory region may be important for regulation of Scr by Polycomb- and trithorax-group genes.

(Gindhart and Kaufman, 1995)

Drosophila has a second N-ethylmaleimide-sensitive fusion protein (NSF) homolog, Nsf2. comt and Nsf2 display overlapping but different temporal expression patterns. Multiple transcripts derived from Nsf2.

(Pallanck et al., 1995)

urd is one of the 18 loci identified in a screen for dominant modifiers of Pc and/or Antp phenotypes. Cytological map position stated to be 87F12-15, derived from complementation analysis with deficiency and duplication chromosomes.

(Kennison and Tamkun, 1988)

Isolated as a dominant suppressor of Pc mutations. Also suppresses Pcl and Msc alleles.

Relationship to Other Genes

Source for database merge of

Source for merge of: Nsf2 urd lotus

(Song et al., 2013)

Additional comments

Annotation CG9931 split into CG33101 (Nsf2) and CG31495 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Nsf2 CG9931

(Zheng, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

CG33101

(Meadows, 2017.1.11, Hosono et al., 2015, Chen et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Zhang et al., 2011, Zhang et al., 2010, Perkins et al., 2009)

CG9931

(Dorer et al., 2006, Dunne et al., 2002, Verstreken and Bellen, 2000.9.4)

DmNSF2

(Frohlich, 2001)

EC3-11

(Baril et al., 2014)

NSF-2

(Lloyd et al., 2000)

Nsf-2

(Junion et al., 2005)

dNSF

(Wu and Bellen, 1997)

dNSF-2

(Mohtashami et al., 2000, Mohtashami and Trimble, 1998, Boulianne and Trimble, 1995, Boulianne and Trimble, 1995, Pallanck, 1995.6.28, Pallanck et al., 1995, Trimble, 1995.6.8)

dNSF2

(Kandasamy et al., 2021, Xu et al., 2014, Lu et al., 2013, Stewart et al., 2005, Babcock et al., 2004, Stewart, 2002, Stewart et al., 2002, Xu et al., 2002, Dunne and Rabouille, 2001, Golby et al., 2001, Stewart et al., 2001, Golby et al., 2000, Tolar et al., 1999)

dNsf2

(Golby et al., 2001)

dnsf2

(Dunne et al., 2002)

lotus

(Song et al., 2013)

urd

(Vasanthi et al., 2013, Kennison, 2012.9.27, Petruk et al., 2008, Vázquez et al., 2008, Chang et al., 2007)

Name Synonyms

N-ethylmaleimide sensitive factor 2

(Song et al., 2013)

N-ethylmaleimide-sensitive factor 2

NEM-sensitive fusion protein 2

(Cammarato et al., 2011, Mika and Rost, 2006, Boulianne and Trimble, 1995)

lotus

(Ghabrial et al., 2011)

urdur

Secondary FlyBase IDs

FBgn0266464
FBgn0264653
FBgn0013998
FBgn0053101
FBgn0003960

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 36 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41694

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P54351

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P54351

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LH53

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P54351

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41694/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0290174

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0290174

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33101

MARRVEL_MODEL - MARRVEL (model organism gene)

41694

Linkouts

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0290174

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (144)

The following external sites may use different assemblies or annotations than FlyBase.

2.73

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