Human Disease Model Report: cancer, malignant glioma, EGFR-MYC-related

General Information

Name

cancer, malignant glioma, EGFR-MYC-related

FlyBase ID

FBhh0000403

Disease Ontology Term

high grade glioma

Parent Disease

OMIM

Overview

This Drosophila model of malignant glioma uses the GAL4/UAS system to drive overexpression of Dmel\Egfr and Dmel\Myc in glial cells and glial precursors. Epidermal growth factor receptor (EGFR) has been implicated in multiple cancers (see FBhh0000398); more than 40% of glioblastomas show amplification and/or mutation of EGFR. Mutations, overexpression, and rearrangements of MYC oncogene have also been implicated in multiple cancers (see FBhh0000402).

EGFR overexpression has been achieved by using a Dmel\Egfr fusion gene that contains a λ dimerization domain; this results in a constitutively active Egfr via forced dimerization. Overexpression of Dmel\Myc has been achieved using a wild-type transgenic copy of the gene; co-overexpression with the Egfr fusion gene induces glial neoplasia in third instar larval brains. This model system has been used to screen for genes that interact genetically to suppress or enhance the glial neoplastic phenotype; see the "Alleles Reported to Model Human Disease" section, below.

[updated Oct. 2016 by FlyBase; FBrf0222196]

Disease Summary Information

Disease Summary: cancer, malignant glioma, EGFR-MYC-related

OMIM report

Human gene(s) implicated

Symptoms and phenotype

Genetics

Cellular phenotype and pathology

Glioblastoma multiforme and other malignant gliomas are characterized by a heterogeneous population of cells that are highly infiltrative, angiogenic and resistant to chemotherapy (Ramirez et al., 2013; pubmed:24287492).

(FlyBase Curators, 2013-)

Glioblastoma multiforme is a malignant astrocytoma and the most common primary brain tumor among adults (reviewed in Furnari et al., 2007; pubmed:17974913).

(FlyBase Curators, 2013-)

Molecular information

MYC encodes a transcription factor that plays a role in cell cycle progression, apoptosis and cellular transformation. [from Gene Cards, MYC; 2016.09.30]

(FlyBase Curators, 2013-)

External links

COSMIC gene: EGFR
COSMIC gene: MYC
Gene2Function (human gene): EGFR
Gene2Function (human gene): MYC
Gene Cards: EGFR
Gene Cards: MYC
MedlinePlus (gene): EGFR
MedlinePlus (gene): MYC
KEGG Disease Pathways: Glioma
MARRVEL (human gene): EGFR
MARRVEL (human gene): MYC
NCBI MedGen: Glioma
NCBI (Entrez) gene: EGFR; epidermal growth factor receptor
NCBI (Entrez) gene: MYC; MYC proto-oncogene, bHLH transcription factor

Disease synonyms

brain cancer, EGFR-MYC-related

cancer, glioblastoma, EGFR-MYC-related

cancer, neuronal, EGFR-MYC-related

glioblastoma

glioma

Related Diseases

Related human health report(s)

cancer, epithelial, EGFR-microRNA-related

cancer, malignant glioma, EGFR-related

cancer, multiple, MYC-related

Ortholog Information

Human gene(s) in FlyBase

Human gene (HGNC)

Symbol / Name

MYC; MYC proto-oncogene, bHLH transcription factor

D. melanogaster ortholog (based on DIOPT)

Dmel\Myc

(DIOPT, 2013-)

Comments on ortholog(s)

Many to one (3 human to 1 Drosophila); additional (lower-scoring) human orthologs are MYCN and MYCL.

Human gene (HGNC)

Symbol / Name

EGFR; epidermal growth factor receptor

D. melanogaster ortholog (based on DIOPT)

Dmel\Egfr

(DIOPT, 2013-)

Comments on ortholog(s)

Many to one (4 human to 1 Drosophila); additional human orthologs are ERBB4, ERBB3, and ERBB2.

Other mammalian ortholog(s) used

D. melanogaster Gene Information (3)

Dmel\Egfr

Gene Snapshot

Epidermal growth factor receptor (Egfr) encodes the transmembrane tyrosine kinase receptor for signaling ligands (encoded by grk, spi, vn, and Krn) in the TGFα family, which utilises the intracellular MAP kinase pathway. The product of Egfr contributes to growth regulation, cell survival and developmental patterning. [Date last reviewed: 2019-06-06]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

EGFR-C
RTK

Comments on ortholog(s)

Orthologous to human genes EGFR, ERBB4, ERBB3, and ERBB2 (1 Drosophila to 4 human). Dmel\Egfr shares 33-37% identity and 46-51% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\Myc

Gene Snapshot

Myc (Myc) encodes a transcription factor that is homologous to vertebrate Myc proto-oncogenes. It contributes to cell growth, cell competition and regenerative proliferation. [Date last reviewed: 2019-03-14]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Orthologous to human gene MYC (reciprocal best hit); lower scoring ortholog of human MYCN and MYCL (1 Drosophila to 3 human). Dmel\Myc shares 31% identity and 44% similarity with human MYC, within the carboxy extents of the two proteins; the amino extents do not align.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\btl::Egfr

Gene Snapshot

Contributions welcome

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Constructs with this fusion gene plus a λ dimerization domain and a UAS promoter produce a constitutively active Egfr via forced dimerization; expression controlled by use of different GAL4 drivers.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Other Genes Used: Viral, Bacterial, Synthetic (0)

Summary of Physical Interactions (131 groups)

Egfr

RNA-protein

Interacting group

Assay

References

Egfr - Fmr1

clip-seq, anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Buddika et al., 2021)

Egfr - pum

rna three hybrid

(Kim et al., 2012)

protein-protein

Interacting group

Assay

References

Egfr

solid phase assay, tag visualisation by fluorescence, anti tag coimmunoprecipitation, anti tag western blot

(Alvarado et al., 2010, Alvarado et al., 2004)

Egfr - Abi

anti bait coimmunoprecipitation, western blot

(Stephan et al., 2008)

Egfr - Abl

two hybrid

(Bianco et al., 2007)

Egfr - arm

anti bait coimmunoprecipitation, western blot

(Dumstrei et al., 2002)

Egfr - ATPsynβ

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Egfr - aux

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Zhao et al., 2022)

Egfr - Btk

two hybrid

(Bianco et al., 2007)

Egfr - Cbl

pull down, anti tag western blot, western blot, anti tag coimmunoprecipitation

(Kim et al., 2017, Hime et al., 1997, Meisner et al., 1997)

Egfr - CG10916

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Friedman et al., 2011, Friedman et al., 2011)

Egfr - Crk

two hybrid

(Bianco et al., 2007)

Egfr - dock

two hybrid

(Bianco et al., 2007)

Egfr - drk

anti bait coimmunoprecipitation, western blot, two hybrid, pull down, anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011, Wang and Pai, 2011, Bianco et al., 2007, MacDougall et al., 2004, Lai et al., 1995, Schweitzer et al., 1995)

Egfr - Egfrap

two hybrid

(Soler Beatty et al., 2021, Bianco et al., 2007)

Egfr - Fas2

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot

(Velasquez et al., 2022)

Egfr - Graf

pull down, anti tag western blot, western blot, anti tag coimmunoprecipitation

(Kim et al., 2017)

Egfr - Hsp83

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Egfr - kek1

enzyme linked immunosorbent assay, anti tag coimmunoprecipitation, western blot, anti tag western blot

(Ozkan et al., 2013, Alvarado et al., 2004, Alvarado et al., 2004, Derheimer et al., 2004, Ghiglione et al., 2003)

Egfr - Lnk

two hybrid

(Bianco et al., 2007)

Egfr - Mbs

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Egfr - Mcad

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Egfr - Pi3K21B

two hybrid

(Bianco et al., 2007)

Egfr - porin

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Egfr - Ptp10D

anti tag coimmunoprecipitation, anti tag western blot

(Jeon and Zinn, 2009)

Egfr - Pyk

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Egfr - RFeSP

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Egfr - Sec10

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Mao et al., 2019)

Egfr - Sec15

anti tag coimmunoprecipitation, western blot, pull down, anti tag western blot

(Mao et al., 2019)

Egfr - Shc

anti tag coimmunoprecipitation, anti tag western blot, two hybrid, anti bait coimmunoprecipitation, western blot, pull down

(Bianco et al., 2007, van der Geer et al., 1996, Lai et al., 1995)

Egfr - shg

anti bait coimmunoprecipitation, western blot

(Dumstrei et al., 2002)

Egfr - spi

surface plasmon resonance, x-ray crystallography, cosedimentation in solution, predetermined participant, solid phase assay, tag visualisation by fluorescence

(Alvarado et al., 2010, Miura et al., 2006, Klein et al., 2004)

Egfr - sqd

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Egfr - Src42A

two hybrid

(Bianco et al., 2007)

Egfr - vap

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Rowshanravan et al., 2014, Bianco et al., 2007)

Egfr - Vav

anti bait coimmunoprecipitation, western blot, two hybrid, anti tag coimmunoprecipitation, peptide massfingerprinting

(Fernández-Espartero et al., 2013, Friedman et al., 2011, Bianco et al., 2007, Sarkar et al., 2007)

Myc

protein-protein

Interacting group

Assay

References

Myc - Act57B

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - ago

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot, tandem affinity purification, Identification by mass spectrometry, two hybrid

(Thevenon et al., 2020, Moberg et al., 2004)

Myc - α-Spec

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - ana2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Arc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - ash2

anti bait coimmunoprecipitation, western blot

(Secombe et al., 2007)

Myc - Atac3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - atms

anti tag coimmunoprecipitation, anti tag western blot

(Gerlach et al., 2017)

Myc - Atu

anti bait coimmunoprecipitation, anti tag western blot, proximity ligation assay, fluorescence microscopy, pull down, autoradiography, anti tag coimmunoprecipitation, western blot

(Gerlach et al., 2017)

Myc - bif

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Brf

anti bait coimmunoprecipitation, western blot, pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot

(Marshall et al., 2012, Steiger et al., 2008)

Myc - brm

anti bait coimmunoprecipitation, western blot

(Secombe et al., 2007)

Myc - CaMKII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Capr

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - CCT1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - CG1620

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - CG13775

two hybrid array

(Shokri et al., 2019)

Myc - CG30122

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - CG31550

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - CG34132

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - cindr

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - CkIIα

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Ctf4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Ctr9

anti tag coimmunoprecipitation, anti tag western blot

(Gerlach et al., 2017)

Myc - Ets21C

two hybrid array

(Shokri et al., 2019)

Myc - fliI

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - gro

pull down, autoradiography, anti tag western blot, anti bait coimmunoprecipitation, western blot

(Orian et al., 2007)

Myc - gw

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - hang

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Hcf

pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation

(Furrer et al., 2010)

Myc - Hsc70-3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - hyd

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - hyx

anti tag coimmunoprecipitation, western blot

(Gerlach et al., 2017)

Myc - iPLA2-VIA

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Kdm5

pull down, autoradiography, anti bait coimmunoprecipitation, western blot

(Li et al., 2010, Secombe et al., 2007)

Myc - kst

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - l(2)09851

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - larp

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - lqfR

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - lva

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - mask

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Max

anti tag coimmunoprecipitation, anti tag western blot, pull down, autoradiography, Identification by mass spectrometry

(Vinayagam et al., 2016, Steiger et al., 2008, Orian et al., 2007)

Myc - Mlc-c

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - msn

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - nito

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Ns3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Nup50

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - org-1

two hybrid array

(Shokri et al., 2019)

Myc - Osbp

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - pcm

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - PIP4K

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - pont

anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation

(Bellosta et al., 2005)

Myc - Prp19

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - puf

anti bait coimmunoprecipitation, western blot

(Li et al., 2013)

Myc - rept

anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation

(Bellosta et al., 2005)

Myc - rin

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL7A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL10Ab

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL13A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL17

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL18A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL19

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL23A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL28

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpL30

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpLP1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpLP2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpS6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpS7

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpS9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpS16

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpS17

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - RpS23

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - rump

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Sag1

two hybrid array

(Shokri et al., 2019)

Myc - Sarm

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - scny

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot

(Thevenon et al., 2020)

Myc - sip2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - smid

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - ssx

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - sta

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - TBPH

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - toy

two hybrid array

(Shokri et al., 2019)

Myc - Usp10

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - Vha26

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - wuho

anti tag coimmunoprecipitation, western blot

(Rastegari et al., 2020)

Myc - yps

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Myc - zda

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RNA-protein

Interacting group

Assay

References

Myc - AGO1

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Malik, 2021)

Myc - bam

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Malik, 2021)

Myc - BicC

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Gamberi et al., 2017)

Myc - brat

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, anti tag coimmunoprecipitation, primer specific pcr

(Malik, 2021, Loedige et al., 2015)

RNA-RNA

Interacting group

Assay

References

Myc - mir-308

western blot, quantitative reverse transcription pcr

(Daneshvar et al., 2013)

Alleles Reported to Model Human Disease (Disease Ontology) (33 alleles)

Egfr

Models Based on Experimental Evidence ( 6 )

Allele

Disease

Evidence

References

Egfr^2.A887T.UAS

model of intestinal cancer

CEA

(Nászai et al., 2021)

model of cancer

CEA with scrib⁶⁷³

(Sheng and Du, 2020)

Egfr^UAS.cBa

model of carcinoma

CEA with Socs36E^GD8929

(Xu et al., 2022, Groth et al., 2020, Herranz et al., 2012)

CEA with mir-ban^C.UAS.EGFP

(Herranz et al., 2012)

CEA with Socs36E^EY11

(Herranz et al., 2012)

CEA

(Groth et al., 2020)

CEA with psq^KK111691

(Boukhatmi et al., 2020, Fereres et al., 2019)

model of cancer

CEA with psq^KK111691

(Herranz et al., 2014)

CEA with mir-8^UAS.cUa

(Eichenlaub et al., 2016)

CEA with mir-ban^UAS.cUa

(Eichenlaub et al., 2016)

CEA with Aldh7A1^KK109006

(Andrejeva et al., 2018)

DOES NOT model carcinoma

CEA with Diap1^UAS.cUa

(Herranz et al., 2012)

model of alcohol use disorder

CEA

(Corl et al., 2009)

model of intestinal cancer

CEA

(Nászai et al., 2021)

Egfr^U.A887T.UAS

model of cardiomyopathy

CEA

(Yu et al., 2013)

Egfr^GD1654

model of alcohol use disorder

CEA

(Corl et al., 2009)

Egfr^KK100051

model of renal fibrosis

CEA with DopEcR^KK111211

(Zheng et al., 2020)

Egfr^Act.cUa.UAS

model of high grade glioma

CEA with Pi3K92E^CAAX.UAS

(Pryszlak et al., 2021)

Modifiers Based on Experimental Evidence ( 16 )

Allele

Disease

Interaction

References

Egfr^{FRT.RNAi.slbo}

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cJa

(Rousseaux et al., 2018)

Egfr^1.UAS

exacerbates cancer

modeled by Myc^UAS.cZa, p53^{RNAi.UAS.RasG12D}

(Diaz et al., 2024)

Egfr^2.A887T.UAS

model of intestinal cancer

is ameliorated by Rala^KK108989

(Nászai et al., 2021)

is exacerbated by Rala^UAS.cRa

(Nászai et al., 2021)

Egfr^2.UAS

exacerbates cancer

modeled by Ras85D^{G12V.UAS.Tag:FLAG,Tag:polyHis}

(Washington et al., 2020)

modeled by Ras85D^{Y4F.G12V.UAS.Tag:FLAG,Tag:polyHis}

(Washington et al., 2020)

Egfr^DN.UAS

ameliorates gastrointestinal system cancer

modeled by Apc2^g10, Apc^Q8

(Wang et al., 2013)

ameliorates colorectal cancer

modeled by Src64B^UAS.cCa

(Cordero et al., 2014)

ameliorates carcinoma

modeled by scrib¹

(Brumby and Richardson, 2003)

Egfr^UAS.cBa

ameliorates spinocerebellar ataxia type 13

modeled by Hsap\KCNC3^R423H.UAS

(Khare et al., 2017)

model of intestinal cancer

is ameliorated by Rala^KK108989

(Nászai et al., 2021)

is exacerbated by Rala^UAS.cRa

(Nászai et al., 2021)

is ameliorated by Ras85D^KK108029

(Nászai et al., 2021)

Egfr^f2

exacerbates germ cell cancer

modeled by sfl^HMS00543

(Levings and Nakato, 2018)

Egfr^f24

ameliorates X-linked mental retardation with cerebellar hypoplasia and distinctive facial appearance

modeled by Graf¹

(Kim et al., 2021)

Egfr^t1

ameliorates colorectal cancer

modeled by Src42A^CA.UAS

(Cordero et al., 2014)

Egfr^JF01368

ameliorates colorectal cancer

modeled by SH3PX1^HMJ21552

(Zhang et al., 2019)

ameliorates glioblastoma

modeled by Pi3K92E^CAAX.UAS, btl::Egfr^λ.UAS

(Chi et al., 2019)

Egfr^UAS.cUa

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cheng et al., 2021)

Egfr^GD1654

ameliorates prostate cancer

modeled by Ras85D^V12.UAS

(Rambur et al., 2020)

Egfr^KK100051

ameliorates colorectal cancer

modeled by Src42A^CA.UAS

(Cordero et al., 2014)

exacerbates alcohol use disorder

modeled by DopEcR^c02142

(Petruccelli et al., 2016)

Egfr^DN.cUa.UAS

DOES NOT ameliorate cancer

modeled by Hipk^UAS.Tag:HA

(Blaquiere et al., 2018)

Egfr^RNAi.UAS.cUa

ameliorates intestinal cancer

modeled by Src64B^UAS.cUa

(Kohlmaier et al., 2015)

modeled by Src42A^CA.UAS

(Kohlmaier et al., 2015)

Egfr^JF02283

ameliorates lung adenocarcinoma

modeled by Hsap\KIF5B::Hsap\RET^UAS.cDa

(Das and Cagan, 2017)

Myc

Models Based on Experimental Evidence ( 5 )

Allele

Disease

Evidence

References

Myc^UAS.cUa

model of cancer

CEA

(Zhang et al., 2015)

model of neurodegenerative disease

CEA with cry^UAS.cEa

(Jarabo et al., 2022)

Myc^αTub84B.PBb

model of cancer

CEA

(Levayer et al., 2015, de la Cova et al., 2014, Moreno and Basler, 2004)

Myc^UAS.Tag:HA

model of carcinoma

CEA with l(2)gl^HMS01522

(Sollazzo et al., 2022)

model of cancer

CEA

(Sollazzo et al., 2018)

Myc^UAS.cZa

model of brain glioma

CEA with btl::Egfr^λ.UAS

(Read et al., 2009)

model of brain cancer

CEA

(Hofstetter et al., 2024)

model of cancer

CEA

(Diaz et al., 2024)

CEA with p53^{RNAi.UAS.RasG12D}

(Diaz et al., 2024)

CEA with p53^GD4483

(Diaz et al., 2024)

Myc^GD1419

model of cancer

CEA

(Qiao et al., 2020)

Modifiers Based on Experimental Evidence ( 9 )

Allele

Disease

Interaction

References

Myc^HMS01538

exacerbates carcinoma

modeled by salr^UAS.cBa

(Sun et al., 2020)

Myc^KK103869

ameliorates brain cancer

modeled by brat^KK113206

(Rebelo and Homem, 2023)

Myc^αTub84B.PBb

model of cancer

is ameliorated by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Levayer et al., 2015)

is ameliorated by shg^UASp.cUa

(Levayer et al., 2015)

is ameliorated by dia^UASp.EGFP

(Levayer et al., 2015)

is ameliorated by Diap1^UAS.cHa

(Levayer et al., 2015)

is ameliorated by sqh^{RLC.N.GFP(S65T)}

(Levayer et al., 2015)

Myc^UAS.Tag:HA

exacerbates carcinoma

modeled by l(2)gl⁴

(Sollazzo et al., 2022)

Myc^UAS.cZa

ameliorates tuberous sclerosis

modeled by Tsc1^UAS.cTa, gig^UAS.cTa

(Tapon et al., 2001)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Singh et al., 2014)

ameliorates Huntington's disease

modeled by Hsap\HTT^{Q138.UAS.EGFP,mRFP1}

(Singh et al., 2014)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Pragati and Sarkar, 2021)

ameliorates carcinoma

modeled by salr^UAS.cBa

(Sun et al., 2020)

modeled by Hsap\SALL4^UAS.Tag:HA

(Sun et al., 2020)

model of brain cancer

is ameliorated by Spt5^HMS00153

(Hofstetter et al., 2024)

Myc^GD1419

ameliorates cancer

modeled by yki^UAS.cHa

(Ziosi et al., 2010)

ameliorates high grade glioma

modeled by Wdr62^UAS.Tag:HA

(Shohayeb et al., 2020)

ameliorates brain cancer

modeled by brat^HMS01121

(Diaz et al., 2024)

Myc^JF01762

ameliorates cystic kidney disease

modeled by BicC⁵

(Gamberi et al., 2017)

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Singh et al., 2014)

Myc^JF01761

ameliorates cystic kidney disease

modeled by BicC⁵

(Gamberi et al., 2017)

ameliorates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Pragati et al., 2020)

ameliorates frontotemporal dementia

modeled by Hsap\MAPT^V337M.UAS

(Pragati et al., 2020)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Pragati and Sarkar, 2021)

Myc^RNAi.UAS.cUa

ameliorates high grade glioma

modeled by aPKC^{UAS.Tag:CAAX(Unk)}

(Paglia et al., 2017)

btl::Egfr

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

btl::Egfr^λ.UAS

model of high grade glioma

CEA with Pi3K92E^CAAX.UAS

(Courant et al., 2022, Ariss et al., 2020)

CEA with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Jarabo et al., 2022, Losada-Pérez et al., 2022, Conte et al., 2021, Jarabo et al., 2021, Maravat et al., 2021, Vigneswaran et al., 2021, Portela et al., 2020, Chen et al., 2019, Portela et al., 2019, Portela et al., 2019, Agnihotri et al., 2016)

CEA

(Bertrand et al., 2023, Chen et al., 2019, Witte et al., 2009)

model of brain glioma

CEA with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2013, Read et al., 2009)

CEA with Myc^UAS.cZa

(Read et al., 2009)

model of brain cancer

CEA

(Richter et al., 2011)

model of glioblastoma

CEA with Pi3K92E^CAAX.UAS

(Chi et al., 2019)

CEA with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Park et al., 2017)

model of intestinal cancer

CEA

(Nászai et al., 2021)

model of prostate cancer

CEA

(Vialat et al., 2024)

model of neurodegenerative disease

CEA with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Jarabo et al., 2022)

CEA with cry^UAS.cEa

(Jarabo et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

btl::Egfr^λ.UAS

exacerbates high grade glioma

modeled by Pi3K92E^UAS.cUa

(Witte et al., 2009)

model of high grade glioma

is exacerbated by Pi3K92E^UAS.cUa

(Witte et al., 2009)

is exacerbated by Drak^UAS.cCa

(Chen et al., 2019)

is ameliorated by Drak^del

(Chen et al., 2019)

is exacerbated by sqh^DD.UAS

(Chen et al., 2019)

model of intestinal cancer

is ameliorated by Rala^KK108989

(Nászai et al., 2021)

model of prostate cancer

is ameliorated by LRP1^JF01628

(Vialat et al., 2024)

is ameliorated by LpR2^HMS03722

(Vialat et al., 2024)

is ameliorated by Npc1a^HMS01646

(Vialat et al., 2024)

Hsap\MYC

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Hsap\MYC^UAS.1

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Raj and Sarkar, 2017)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Raj and Sarkar, 2017)

Hsap\MYC^UAS.2

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Raj and Sarkar, 2017)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Raj and Sarkar, 2017)

modeled by Hsap\HTT^{Q138.UAS.mRFP1}

(Raj and Sarkar, 2017)

Hsap\MYC^UAS.S

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Raj and Sarkar, 2017)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Raj and Sarkar, 2017)

Hsap\EGFR

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Hsap\EGFR^Δ.UAS

model of brain glioma

CEA with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2013)

CEA

(Read et al., 2013)

model of high grade glioma

CEA with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Terrasi et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Hsap\EGFR^Δ.UAS

model of brain glioma

is ameliorated by RIOK1^GD8541

(Read et al., 2013)

is ameliorated by RIOK2^KK101850

(Read et al., 2013)

is ameliorated by RIOK1^KK100810

(Read et al., 2013)

is ameliorated by RIOK2^GD7407

(Read et al., 2013)

Alleles Representing Disease-Implicated Variants

Genetic Tools, Stocks and Reagents

Sources of Stocks

Contact lab of origin for a reagent not available from a public stock center.

Bloomington Stock Center Disease Page

Related mammalian, viral, bacterial, or synthetic transgenes

Allele

Transgene

Publicly Available Stocks

Selected Drosophila transgenes

Allele

Transgene

Publicly Available Stocks

y¹ w^*; P{UAS-Egfr.B}32-26-1

Egfr^2.UAS

P{Egfr.2.UAS}

w^*; P{Egfr.2.UAS}7-10

Egfr^2.A887T.UAS

P{Egfr.2.A887T.UAS}

w^*; P{Egfr.2.A887T.UAS}8-2

P{UAS-Egfr.lambdatop}

w^*; P{UAS-Egfr.λtop}3/TM6C, Sb¹

Myc^UAS.cZa

P{UAS-Myc.Z}

w¹¹¹⁸; P{UAS-Myc.Z}132

Myc^αTub84B.PBb

P{alphaTub84B(FRT.Myc)lexA::GAD.A}

P{ey-FLP.N}2, w^* P{P-Sal}Myc^P0 P{αTub84B(FRT.Myc)GAL4.Bb}1/FM7a; rept³⁵/MKRS

RNAi constructs available

Allele

Transgene

Publicly Available Stocks

Egfr^{RNAi.13xlexAop.cZa}

P{13xlexAop-Egfr.RNAi.Z}

Egfr^GD1654

P{GD1654}

w¹¹¹⁸; P{GD1654}v43267

Egfr^JF01368

P{TRiP.JF01368}

y¹ v¹; P{TRiP.JF01368}attP2

y¹ v¹; P{TRiP.JF01083}attP2

Egfr^JF01084

P{TRiP.JF01084}

y¹ v¹; P{TRiP.JF01084}attP2

Egfr^JF01696

P{TRiP.JF01696}

y¹ v¹; P{TRiP.JF01696}attP2

y¹ v¹; P{TRiP.JF02283}attP2

Egfr^JF02384

P{TRiP.JF02384}

y¹ v¹; P{TRiP.JF02384}attP2

Egfr^{FRT.RNAi.slbo}

M{slbo(FRT.lacZ)Egfr.RNAi}

w¹¹¹⁸; M{slbo(FRT.lacZ)Egfr.RNAi}ZH-51D

Egfr^RNAi.slbo

M{slbo(-FRT)Egfr.RNAi}

y¹ v¹; P{TRiP.HMS05003}attP40

y¹ v¹; P{TRiP.HMJ22067}attP40/CyO

Myc^{RNAi.13xlexAop.cZa}

P{13xlexAop-Myc.RNAi.Z}

Myc^GD1419

P{GD1419}

w¹¹¹⁸; P{GD1419}v2947

Myc^JF01761

P{TRiP.JF01761}

y¹ v¹; P{TRiP.JF01761}attP2

Myc^JF01762

P{TRiP.JF01762}

y¹ v¹; P{TRiP.JF01762}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01538}attP2

Myc^GL01314

P{TRiP.GL01314}

y¹ sc^* v¹ sev²¹; P{TRiP.GL01314}attP40

Myc^HMC03189

P{TRiP.HMC03189}

y¹ v¹; P{TRiP.HMC03189}attP40/CyO

Selected Drosophila classical alleles

Allele

Allele class

Mutagen

Publicly Available Stocks

Egfr^top-EB

loss of function allele

ethyl methanesulfonate

Egfr^f24

loss of function allele

ethyl methanesulfonate

w^*; smo^D16 P{neoFRT}42D mam¹⁰ Egfr^f24/CyO, P{ActGFP}JMR1

Egfr^f36

amorphic allele - genetic evidence

gamma ray

Egfr^top-101

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^top-EE42

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^top-EC20

loss of function allele

ethyl methanesulfonate

Egfr^f3

loss of function allele

ethyl methanesulfonate

cn¹ Egfr^f3 bw¹ speck¹/CyO

Egfr^f6

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^f9

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^f11

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^top-EA

loss of function allele

ethyl methanesulfonate

Egfr^top-CA

loss of function allele

ethyl methanesulfonate

Egfr^top-38

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^f8

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^JE1

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^f38

amorphic allele - genetic evidence

gamma ray

Egfr^JE13

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^f12

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^f10

amorphic allele - genetic evidence

ethyl methanesulfonate

Egfr^f5

amorphic allele - genetic evidence

ethyl methanesulfonate

cn¹ Egfr^f5 bw¹ speck¹/CyO

Egfr^top-18A

amorphic allele - genetic evidence

Egfr^f37

amorphic allele - genetic evidence

gamma ray

Egfr^t1

ethyl methanesulfonate

Egfr^t1 bw¹/CyO

Egfr^f2

amorphic allele - genetic evidence

ethyl methanesulfonate

cn¹ Egfr^f2 bw¹ speck¹/CyO

Myc^A51

amorphic allele - molecular evidence

P-element activity

References (8)

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