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General Information
Name
prostate cancer, E-cadherin-related
FlyBase ID
FBhh0000457
Disease Ontology Term
Parent Disease
OMIM
Overview

This model of prostate cancer is based on the observation that the Drosophila adult male accessory gland acts as a functional homolog of the mammalian prostate. It was hypothesized that normal cell growth and migration of secondary cells in the accessory gland may be regulated by Drosophila orthologs of known regulators of human prostate cancer progression. A number of the genes in this category are highly expressed in the male accessory gland; for several these, including shg, knockdown via RNAi was shown to result in changes in the number or migration of the secondary cells. (FBrf0226167)

Cadherins are a diverse group of transmembrane receptors which mediate cell-cell adhesion and cell movement. Dmel\shg is one of multiple cadherins in flies; there are also many cadherins in human. A low-scoring ortholog of Dmel\shg, human CDH1, has been implicated in susceptibility to prostate cancer (OMIM:192090).

Amorphic mutations of Dmel\shg result in embryonic lethality. For assessment of function in the accessory gland, RNAi-mediated knockdown of shg was controlled by using a temperature-sensitive driver and targeting newly eclosed, newly mated males. Knockdown of shg results in an increase in secondary cell migration in the accessory gland; the phenotype is variable with incomplete penetrance. Physical and genetic interactions of Dmel\shg have been characterized; see below and in the gene report for shg.

[updated Dec. 2016 by FlyBase; FBrf0222196]

Disease Summary Information
Parent Disease Summary: prostate cancer
Symptoms and phenotype

Prostate cancer is the most common cancer in American men and is usually very slowly progressive; it is diagnosed in an estimated 80% of men who reach age 80 (http://www.webmd.com/prostate-cancer; 2016.12.20).

Specific Disease Summary: prostate cancer, E-cadherin-related
OMIM report
Human gene(s) implicated
Symptoms and phenotype
Genetics
Cellular phenotype and pathology
Molecular information

The cadherin ("calcium-dependent adhesion") superfamily represents a diverse group of transmembrane receptors which mediate cell-cell adhesion and cell movement. (FBrf0146675)

See 'Gene Family: Cadherins' (http://www.genenames.org/cgi-bin/genefamilies/set/16) and 'Gene Family: Type II classical cadherins (CDH)' (http://www.genenames.org/cgi-bin/genefamilies/set/1186).

External links
Disease synonyms
Ortholog Information
Human gene(s) in FlyBase
    Human gene (HGNC)
    Symbol / Name
    D. melanogaster ortholog (based on DIOPT)
    Comments on ortholog(s)

    Many to many (not all listed).

    Other mammalian ortholog(s) used
      D. melanogaster Gene Information (1)
      Gene Groups / Pathways
      Comments on ortholog(s)

      Low-scoring ortholog of human type II cadherin CDH20 (most closely related) and multiple others (many Drosophila to many human), including CDH1. Dmel\shg shares 19% identity and 29% similarity with human CDH20.

      Orthologs and Alignments from DRSC
      DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
      Synthetic Gene(s) Used (0)
      Summary of Physical Interactions (19 groups)
      protein-protein
      Interacting group
      Assay
      References
      bead aggregation assay, fluorescence microscopy, inferred by author
      anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot
      anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, two hybrid, fluorescent resonance energy transfer, fluorescence microscopy, pull down, autoradiography, molecular weight estimation by staining
      anti bait coimmunoprecipitation, western blot
      pull down, molecular weight estimation by staining
      anti bait coimmunoprecipitation, western blot
      anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot
      two hybrid, anti bait coimmunoprecipitation, western blot
      anti tag coimmunoprecipitation, western blot
      anti tag coimmunoprecipitation, western blot
      enzymatic study, molecular weight estimation by staining
      enzymatic study, molecular weight estimation by staining
      two hybrid, anti bait coimmunoprecipitation, western blot, pull down, autoradiography
      anti tag coimmunoprecipitation, western blot
      anti bait coimmunoprecipitation, western blot
      two hybrid, pull down, autoradiography, anti tag coimmunoprecipitation, western blot
      anti bait coimmunoprecipitation, western blot
      RNA-protein
      Interacting group
      Assay
      References
      iclip, partial RNA sequence identification
      electrophoretic mobility shift assay, tag visualisation, anti bait coimmunoprecipitation, quantitative reverse transcription pcr, reverse transcription pcr, nucleic acid uv cross-linking assay
      Alleles Reported to Model Human Disease (Disease Ontology) (0 alleles)
      Genetic Tools, Stocks and Reagents
      Sources of Stocks
      Contact lab of origin for a reagent not available from a public stock center.
      Bloomington Stock Center Disease Page
      Selected mammalian transgenes
      Allele
      Transgene
      Publicly Available Stocks
      Selected Drosophila transgenes
      Allele
      Transgene
      Publicly Available Stocks
      RNAi constructs available
      Allele
      Transgene
      Publicly Available Stocks
      Selected Drosophila classical alleles
      Allele
      Allele class
      Mutagen
      Publicly Available Stocks
      loss of function allele
      ends-out gene targeting
      loss of function allele
      ends-out gene targeting
      loss of function allele
      ends-out gene targeting
      loss of function allele
      ends-out gene targeting
      loss of function allele
      P-element activity
      loss of function allele
      P-element activity
      loss of function allele
      gamma ray
      amorphic allele - genetic evidence
      ethyl methanesulfonate
      loss of function allele
      loss of function allele
      ethyl methanesulfonate
      loss of function allele
      loss of function allele
      amorphic allele - genetic evidence
      loss of function allele
      P-element activity
      References (4)