This report describes characterization of the fly response to alcohol using the Drosophila gene arouser (aru). Dmel\aru is an SH3 adaptor protein with orthology to the four EPS8 (Epidermal Growth Factor Receptor Pathway Substrate 8) and EPS8-like genes in human. RNAi targeting constructs and alleles caused by insertional mutagenesis have been generated for Dmel\aru. There is a second gene from this family in Drosophila, CG8907.
One of the human EPS8 genes, Hsap\EPS8L2, has been introduced into flies, but has not been characterized.
In a genetic screen for mutants with altered sensitivity to ethanol sedation, an insertion allele of aru exhibited increased ethanol sensitivity; a second insertion allele was also found to have an increased sensitivity phenotype. Both alleles produce a sensitivity phenotype in heterozygous animals. A small number of genetic and physical interactions have been described for Dmel\aru; see below and in the aru gene report.
Since the human EPS8 genes are implicated in both EGFR/ERK and P13K/AKT signaling pathways, additional mutations in Drosophila genes within these pathways have been assessed for ethanol sensitivity; see FBhh0000685 and FBhh0000689.
[updated Jan. 2018 by FlyBase; FBrf0222196]