Open Close
General Information
Name
anxiety modulator(s), serotonin class 2 receptor(s)
FlyBase ID
FBhh0001006
Disease Ontology Term
Parent Disease
OMIM
Overview

Using the Drosophila wall-following assay (WAFO; see FBhh0001005), the roles of serotonin class 2 receptors in the modulation of anxiety were assessed. There are two serotonin class 2 receptor genes in flies. Knockdown of Dmel\5-HT2B was observed to significantly increase WAFO (interpreted as increased anxiety); knockdown of Dmel\5-HT2A had no consistent effect. RNAi-targeting constructs, alleles caused by insertional mutagenesis, and an amorphic allele created by targeted recombination have been generated for both Dmel\5-HT2B and Dmel\5-HT2A.

There are three human serotonin class 2 receptor genes. Mutations in the human HTR2A gene are associated with susceptibility to schizophrenia, obsessive-compulsive disorder, and other psychiatric conditions; variants in this gene also affect response to specific antidepressants. None of the human HTR2 genes has been introduced into flies.

The fly 5-HT2A and 5-HT2B genes have not been extensively characterized. As in the WAFO assay, the two genes exhibit different responses in an assay assessing resistance to a drug that suppresses feeding: animals with loss-of-function genotypes of Dmel\5-HT2A are more resistant than wild-type to the drug metitepine, while the response of animals with an amorphic mutation of Dmel\5-HT2B is similar to wild-type. See the human disease model report 'obesity, susceptibility to (postulated), serotonin class 2 receptor(s)' (FBhh0001104).

WAFO experiments using the Drosophila serotonin class 1 receptor gene 5-HT1B are described in the human disease model report 'anxiety modulators, fly wall-following model' (FBhh0001005). Reciprocal effects are observed: knockdown of the fly gene results in increased WAFO, overexpression results in decreased WAFO.

[updated Jul. 2019 by FlyBase; FBrf0222196]

Disease Summary Information
Disease Summary: anxiety modulator(s), serotonin class 2 receptor(s)
OMIM report
Human gene(s) implicated
Symptoms and phenotype
Variants in the human HTR2A gene are associated with susceptibility to schizophrenia, obsessive-compulsive disorder, and other psychiatric conditions; variants in this gene also affect response to specific antidepressants. [NCBI Gene, HTR2A, 2019.04.17; OMIM:182135, 2019.04.17]
Genetics
There are 13 G-protein-coupled serotonin receptor genes in human, comprising 6 subclasses (HGNC: https://www.genenames.org/data/genegroup/#!/group/170). There are three human serotonin class 2 receptor genes.
There are 5 G-protein-coupled serotonin receptor genes in Drosophila, making up 3 subclasses (corresponding to subclasses 1, 2 and 7 in human) (see FBgg0000206). There are two serotonin class 2 receptor genes in fly.
Cellular phenotype and pathology
Molecular information
HTR2A, HTR2B, and HTR2C encode G-protein-coupled receptors for the neurotransmitter 5-hydroxytryptamine (5-HT or serotonin).
External links
Disease synonyms
Ortholog Information
Human gene(s) in FlyBase
    Other mammalian ortholog(s) used
      D. melanogaster Gene Information (1)
      Gene Snapshot
      5-hydroxytryptamine (serotonin) receptor 2B (5-HT2B) encodes a 5-hydroxytryptamine (serotonin) GPCR that belongs to the Class A GPCR family. They bind and transmit the signal from the neurotransmitter 5-HT (serotonin). The family can be subdivided into several subclasses based on pharmacology, signal transduction and structure. There are 5 genes encoding serotonin receptors in the Drosophila melanogaster genome: 5-HT1A, 5-HT1B, 5-HT2A, 5-HT2B, and 5-HT7. [Date last reviewed: 2019-09-26]
      Gene Groups / Pathways
      Comments on ortholog(s)
      Low-scoring ortholog of human HTR2A, HTR2B, and HTR2C (2 Drosophila to 3 human). Dmel\5-HT2B shares 18-19% identity and 28% similarity with the human genes. (Dmel\5-HT2A is somewhat more closely related to the human genes.)
      Orthologs and Alignments from DRSC
      DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
      Synthetic Gene(s) Used (0)
      Summary of Physical Interactions (0 groups)
      Alleles Reported to Model Human Disease (Disease Ontology) (0 alleles)
      Genetic Tools, Stocks and Reagents
      Sources of Stocks
      Contact lab of origin for a reagent not available from a public stock center.
      Bloomington Stock Center Disease Page
      Selected mammalian transgenes
      Allele
      Transgene
      Publicly Available Stocks
      Selected Drosophila transgenes
      Allele
      Transgene
      Publicly Available Stocks
      RNAi constructs available
      Allele
      Transgene
      Publicly Available Stocks
      Selected Drosophila classical alleles
      Allele
      Allele class
      Mutagen
      Publicly Available Stocks
      amorphic allele - molecular evidence
      gene targeting by homologous recombination
      References (3)