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General Information
Name
cancer, epithelial, STAG1,2-related
FlyBase ID
FBhh0001288
Disease Ontology Term
Parent Disease
OMIM
Overview

This report is based upon experiments in Drosophila designed to identify genes involved in later stages of cancer development, including cellular acquisition of invasive behavior. The Drosophila gene SA was identified in this screen. Dmel\SA encodes encodes a subunit of the cohesin ring complex; there is a paralogous gene in Drosophila, SA-2. In an additional assay, clones of SA or SA-2 induced in epithelial tissue in a wild-type background (without a potentiating mutation) also showed invasive phenotypes.

A limited number of genetic reagents are available for SA including a loss-of-function mutation and several RNAi-targeting constructs. Multiple genetic reagents have been generated for SA-2 including loss-of-functions mutations, RNAi-targeting constructs, and an anoverexpression construct. SA-2 is expressed primarily in testis.

Dmel\SA is orthologous to three human genes, STAG1, STAG2, and STAG3. None of the human genes has been introduced into flies. Using a breast cancer cell line, the effects of knockdown of STAG1 or STAG2 were assessed; in both cases a significant increase in both invasion and migration was observed. The STAG3 gene was not tested in this assay.

[updated Nov. 2020 by FlyBase; FBrf0222196]

Disease Summary Information
Disease Summary: cancer, epithelial, STAG1,2-related
OMIM report
Human gene(s) implicated
Symptoms and phenotype
Genetics
Cellular phenotype and pathology
Molecular information

STAG1 and STAG2 encode components of the cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication; the cohesin complex may also play a role in spindle pole assembly during mitosis. [Gene Cards, STAG1, STAG2; 2020.11.28]

External links
Disease synonyms
Ortholog Information
Human gene(s) in FlyBase
    Human gene (HGNC)
    D. melanogaster ortholog (based on DIOPT)
    Comments on ortholog(s)

    Many to many: 3 human genes to 2 Drosophila genes.

    Human gene (HGNC)
    D. melanogaster ortholog (based on DIOPT)
    Comments on ortholog(s)

    Many to many: 3 human genes to 2 Drosophila genes.

    Other mammalian ortholog(s) used
      D. melanogaster Gene Information (2)
      Gene Snapshot
      Stromalin-2 (SA-2) encodes a paralog of the cohesin protein encoded by SA. It functions in the male meiosis-specific homolog pairing pathway. It is required to maintain homolog pairing and to ensure bi-orientation of homologs during meiosis I. [Date last reviewed: 2019-03-14]
      Molecular function (GO)
      Gene Groups / Pathways
        Comments on ortholog(s)

        Moderate-scoring ortholog of human STAG1, STAG2, and STAG3 (2 Drosophila to 3 human). Dmel\SA-2 shares 24-25% identity and 42-44% similarity with the human genes.

        Orthologs and Alignments from DRSC
        DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
        Gene Snapshot
        Stromalin (SA) encodes a subunit of the cohesin ring complex, which encircles sister chromatids after DNA replication and has multiple roles in the regulation of gene expression and DNA repair. [Date last reviewed: 2019-03-14]
        Molecular function (GO)
        Gene Groups / Pathways
        Comments on ortholog(s)

        High-scoring ortholog of human STAG1, STAG2, and STAG3 (2 Drosophila to 3 human). Dmel\SA shares 39-46% identity and 60-66% similarity with the human genes.

        Orthologs and Alignments from DRSC
        DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
        Synthetic Gene(s) Used (0)
        Summary of Physical Interactions (12 groups)
        protein-protein
        Interacting group
        Assay
        References
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        protein-protein
        Interacting group
        Assay
        References
        ion exchange chromatography, peptide massfingerprinting
        anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
        pull down, molecular weight estimation by staining
        anti bait coimmunoprecipitation, western blot
        anti bait coimmunoprecipitation, western blot, pull down, autoradiography
        anti tag coimmunoprecipitation, anti tag western blot
        pull down, autoradiography
        anti tag coimmunoprecipitation, western blot
        anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot
        anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot, anti tag western blot
        Alleles Reported to Model Human Disease (Disease Ontology) (0 alleles)
        Genetic Tools, Stocks and Reagents
        Sources of Stocks
        Contact lab of origin for a reagent not available from a public stock center.
        Bloomington Stock Center Disease Page
        Selected mammalian transgenes
        Allele
        Transgene
        Publicly Available Stocks
        Selected Drosophila transgenes
        Allele
        Transgene
        Publicly Available Stocks
        RNAi constructs available
        Allele
        Transgene
        Publicly Available Stocks
        Selected Drosophila classical alleles
        Allele
        Allele class
        Mutagen
        Publicly Available Stocks
        loss of function allele
        ethyl methanesulfonate
        loss of function allele
        ethyl methanesulfonate
        References (3)