FB2025_02 , released April 17, 2025
Human Disease Model Report: cancer, multiple, TP53-MYC-related
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General Information
Name
cancer, multiple, TP53-MYC-related
FlyBase ID
FBhh0001602
Disease Ontology Term
Parent Disease
OMIM
Overview

A model of early steps in genetically complex cancer types has been developed using overexpression of the Drosophila gene Dmel\Myc in combination with loss-of-function for Dmel\p53. See also the human disease model reports 'cancer, multiple, TP53-related' (FBhh0000340) and 'cancer, multiple, MYC-related' (FBhh0000402).

Although the orthologous human genes, Hsap\TP53 and Hsap\MYC, have been introduced into flies, they have not been used in the context of this disease model.

For this TP53-MYC-related cancer model, specific targeting of transgene expression to the anterior/posterior boundary of the wing disc allows sensitive visual assays of transformation. This system has been used to test the additional modification of candidate genes located within copy number alterations commonly observed in triple-negative breast cancer (TNBC). Each candidate gene was tested in the p53 mutant, Myc overexpression background: amplified genes were assessed by overexpression of the Drosophila ortholog; deleted genes were assessed by RNAi-mediated knockdown or by removing one functional copy of the Drosophila ortholog. Six of the positive '3-hit' models were subsequently assessed for response to a chemotherapeutic drug; in all cases, increased genetic complexity reduced treatment response.

[updated Sep. 2024 by FlyBase; FBrf0222196]

Disease Summary Information
Disease Summary: cancer, multiple, TP53-MYC-related
OMIM report
Human gene(s) implicated
Symptoms and phenotype
Genetics
Cellular phenotype and pathology
Molecular information
External links
Disease synonyms
p53-Myc platform
Ortholog Information
Human gene(s) in FlyBase
Human gene (HGNC)
Symbol / Name
TP53; tumor protein p53
D. melanogaster ortholog (based on DIOPT)
Dmel\p53
(DIOPT, 2013-)
Comments on ortholog(s)

Many to one: 3 human to 1 Drosophila; additional orthologous human genes are TP63 and TP73.

Human gene (HGNC)
Symbol / Name
MYC; MYC proto-oncogene, bHLH transcription factor
D. melanogaster ortholog (based on DIOPT)
Dmel\Myc
(DIOPT, 2013-)
Comments on ortholog(s)

Many to one: 3 human to 1 Drosophila; additional (lower-scoring) human orthologs are MYCN and MYCL.

Other mammalian ortholog(s) used
    D. melanogaster Gene Information (2)
    Dmel\Myc
    Gene Snapshot
    Myc (Myc) encodes a transcription factor that is homologous to vertebrate Myc proto-oncogenes. It contributes to cell growth, cell competition and regenerative proliferation. [Date last reviewed: 2019-03-14]
    Molecular function (GO)
    Cellular component (GO)
    Gene Groups / Pathways
    Comments on ortholog(s)

    Orthologous to human gene MYC (reciprocal best hit); lower scoring ortholog of human MYCN and MYCL (1 Drosophila to 3 human).

    Orthologs and Alignments from DRSC
    DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
    H. sapiens (Human)
    Dmel\p53
    Gene Snapshot
    p53 (p53) encodes a transcriptional factor required for adaptive responses to genotoxic stress, including cell death, compensatory proliferation and DNA repair. [Date last reviewed: 2019-03-14]
    Molecular function (GO)
    Cellular component (GO)
    Gene Groups / Pathways
    Comments on ortholog(s)

    High-scoring ortholog of human genes TP53, TP63 and TP73 (1 Drosophila to many human).

    Orthologs and Alignments from DRSC
    DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans
    H. sapiens (Human)
    Other Genes Used: Viral, Bacterial, Synthetic (0)
      Summary of Physical Interactions (230 groups)
      Myc
      protein-protein
      Interacting group
      Assay
      References
      Myc - Act57B
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - ago
      anti tag coimmunoprecipitation, anti tag western blot, two hybrid, tandem affinity purification, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot
      (Thevenon et al., 2020, Moberg et al., 2004)
      Myc - α-Spec
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - ana2
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Arc1
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - ash2
      anti bait coimmunoprecipitation, western blot
      (Secombe et al., 2007)
      Myc - Atac3
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - atms
      anti tag coimmunoprecipitation, anti tag western blot
      (Gerlach et al., 2017)
      Myc - Atu
      pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot, proximity ligation assay, fluorescence microscopy, western blot, anti bait coimmunoprecipitation
      (Gerlach et al., 2017)
      Myc - bif
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Brf
      pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot
      (Marshall et al., 2012, Steiger et al., 2008)
      Myc - brm
      anti bait coimmunoprecipitation, western blot
      (Secombe et al., 2007)
      Myc - CaMKII
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Capr
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - CCT1
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - CG1620
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - CG13775
      two hybrid array
      (Shokri et al., 2019)
      Myc - CG30122
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - CG31550
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - CG34132
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - cindr
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - CkIIα
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Ctf4
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Ctr9
      anti tag coimmunoprecipitation, anti tag western blot
      (Gerlach et al., 2017)
      Myc - Ets21C
      two hybrid array
      (Shokri et al., 2019)
      Myc - fliI
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - gro
      anti bait coimmunoprecipitation, western blot, pull down, autoradiography, anti tag western blot
      (Orian et al., 2007)
      Myc - gw
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - hang
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Hcf
      anti bait coimmunoprecipitation, anti tag western blot, pull down, autoradiography, anti tag coimmunoprecipitation
      (Furrer et al., 2010)
      Myc - Hsc70-3
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - hyd
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - hyx
      anti tag coimmunoprecipitation, western blot
      (Gerlach et al., 2017)
      Myc - iPLA2-VIA
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Kdm5
      anti bait coimmunoprecipitation, western blot, pull down, autoradiography
      (Li et al., 2010, Secombe et al., 2007)
      Myc - kst
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - l(2)09851
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - larp
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - lqfR
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - lva
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - mask
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Max
      anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry, pull down, autoradiography
      (Vinayagam et al., 2016, Steiger et al., 2008, Orian et al., 2007)
      Myc - Mlc-c
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - msn
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - nito
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Ns3
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Nup50
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - org-1
      two hybrid array
      (Shokri et al., 2019)
      Myc - Osbp
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - pcm
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - PIP4K
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - pont
      pull down, western blot, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation
      (Bellosta et al., 2005)
      Myc - Prp19
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - puf
      anti bait coimmunoprecipitation, western blot
      (Li et al., 2013)
      Myc - rept
      anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, pull down
      (Bellosta et al., 2005)
      Myc - rin
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL4
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL6
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL7A
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL10Ab
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL13A
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL17
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL18A
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL19
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL23A
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL28
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpL30
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpLP1
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpLP2
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpS6
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpS7
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpS9
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpS16
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpS17
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - RpS23
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - rump
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Sag1
      two hybrid array
      (Shokri et al., 2019)
      Myc - Sarm
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - scny
      anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot
      (Thevenon et al., 2020)
      Myc - sip2
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - smid
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - ssx
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - sta
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - TBPH
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - toy
      two hybrid array
      (Shokri et al., 2019)
      Myc - Usp10
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - Vha26
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - wuho
      anti tag coimmunoprecipitation, western blot
      (Rastegari et al., 2020)
      Myc - yps
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      Myc - zda
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      RNA-protein
      Interacting group
      Assay
      References
      Myc - AGO1
      anti bait coimmunoprecipitation, quantitative reverse transcription pcr
      (Malik, 2021)
      Myc - bam
      anti bait coimmunoprecipitation, quantitative reverse transcription pcr
      (Malik, 2021)
      Myc - BicC
      anti bait coimmunoprecipitation, quantitative reverse transcription pcr
      (Gamberi et al., 2017)
      Myc - brat
      anti bait coimmunoprecipitation, quantitative reverse transcription pcr, anti tag coimmunoprecipitation, primer specific pcr
      (Malik, 2021, Loedige et al., 2015)
      RNA-RNA
      Interacting group
      Assay
      References
      Myc - mir-308
      western blot, quantitative reverse transcription pcr
      (Daneshvar et al., 2013)
      p53
      protein-protein
      Interacting group
      Assay
      References
      p53
      anti tag coimmunoprecipitation, western blot, coimmunoprecipitation, anti tag western blot, pull down, autoradiography
      (Park et al., 2019, Zhang et al., 2015, Lunardi et al., 2010)
      p53 - abd-A
      bimolecular fluorescence complementation, fluorescence microscopy
      (Bischof et al., 2018)
      p53 - Acat1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Ada2b
      anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot
      (Pankotai et al., 2013, Kusch et al., 2003)
      p53 - AGO2
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Ahcy
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - al
      anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
      (Rhee et al., 2014)
      p53 - ap
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - asf1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - asp
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - aub
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Bap60
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - boly
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - bowl
      two hybrid array
      (Shokri et al., 2019)
      p53 - Brd8
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - bsf
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - bsk
      anti tag coimmunoprecipitation, western blot
      (Gowda et al., 2012)
      p53 - Cep89
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG1074
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG1137
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG1602
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG1647
      two hybrid array
      (Shokri et al., 2019)
      p53 - CG1958
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG3610
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG4854
      two hybrid array, anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
      (Shokri et al., 2019, Rhee et al., 2014)
      p53 - CG6325
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG6333
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG6686
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG7139
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG7379
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG7387
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG7634
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG8236
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG8728
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG8851
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG10333
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG11035
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG12861
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG13436
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG15269
      two hybrid array
      (Shokri et al., 2019)
      p53 - CG30059
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - CG32985
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - chb
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - chinmo
      two hybrid array
      (Shokri et al., 2019)
      p53 - Coprox
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Corp
      anti tag coimmunoprecipitation, anti tag western blot, pull down, autoradiography, western blot
      (Chakraborty et al., 2015)
      p53 - cort
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Cp110
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - croc
      two hybrid array
      (Shokri et al., 2019)
      p53 - CycG
      two hybrid, anti tag coimmunoprecipitation, western blot
      (Bayer et al., 2017)
      p53 - Cyp6a22
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Cyp6d5
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Cyt-c1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Daxx
      pull down, autoradiography, two hybrid
      (Lunardi et al., 2010, Bodai et al., 2007)
      p53 - Dlip3
      anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
      (Rhee et al., 2014)
      p53 - Dll
      two hybrid array
      (Shokri et al., 2019)
      p53 - Dph1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - dsx
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - dup
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - dyl
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Fdh
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - fdl
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Gas8
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Gcn5
      anti bait coimmunoprecipitation, western blot, pull down
      (Kusch et al., 2003)
      p53 - grau
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - gry
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - gus
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - hay
      anti bait coimmunoprecipitation, western blot
      (Villicaña et al., 2013)
      p53 - hmw
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Hsf
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Hsp23
      anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
      (Rhee et al., 2014)
      p53 - Hsp26
      anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
      (Lei et al., 2017, Rhee et al., 2014)
      p53 - Hsp27
      anti bait coimmunoprecipitation, western blot
      (Lei et al., 2017)
      p53 - Ice2
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Idi
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - if
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Imp
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - INPP5E
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - jp
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - ktub
      two hybrid array
      (Shokri et al., 2019)
      p53 - lost
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - lwr
      pull down, autoradiography, two hybrid
      (Hu et al., 2017.6.13, Pardi et al., 2011)
      p53 - Mmp2
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - mrj
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - mrn
      pull down, autoradiography, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot
      (Villicaña et al., 2013)
      p53 - mRpL16
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - mtgo
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - mTTF
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - mu2
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - mura
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - ND-51L2
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Nna1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Non1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - nord
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Nprl3
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - pho
      bimolecular fluorescence complementation, fluorescence microscopy, two hybrid array
      (Shokri et al., 2019)
      p53 - PPO2
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - raskol
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - RPA1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - RtcB
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - SA1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Sas-6
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - sima
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - sip3
      enzymatic study, western blot
      (Yamasaki et al., 2007)
      p53 - Sirt1
      anti tag coimmunoprecipitation, western blot
      (Bauer et al., 2009)
      p53 - Sox102F
      two hybrid array
      (Shokri et al., 2019)
      p53 - spoon
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - sqh
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - ssx
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Su(var)2-10
      two hybrid, pull down, autoradiography
      (Pardi et al., 2011)
      p53 - Svil
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Taf3
      two hybrid, pull down, autoradiography
      (Bereczki et al., 2008)
      p53 - Taf11
      anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
      (Rhee et al., 2014)
      p53 - tbrd-1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Tfb1
      anti bait coimmunoprecipitation, western blot
      (Villicaña et al., 2013)
      p53 - Timmdc1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Tm1
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Tom40
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Topors
      pull down, autoradiography
      (Secombe and Parkhurst, 2004)
      p53 - toy
      two hybrid array, pull down, autoradiography
      (Shokri et al., 2019, Lunardi et al., 2010)
      p53 - TTLL5
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - TwdlW
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Uba2
      two hybrid, pull down, autoradiography
      (Pardi et al., 2011)
      p53 - Ubc6
      pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot, coimmunoprecipitation
      (Chen et al., 2011)
      p53 - Ubx
      bimolecular fluorescence complementation, fluorescence microscopy
      (Bischof et al., 2018)
      p53 - uno
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - Usp7
      enzymatic study, western blot
      (van der Knaap et al., 2005)
      p53 - Utx
      anti bait coimmunoprecipitation, western blot
      (Zhang et al., 2013)
      p53 - Vha36-2
      pull down, autoradiography
      (Lunardi et al., 2010)
      p53 - yki
      anti tag coimmunoprecipitation, anti tag western blot
      (Di Cara et al., 2015)
      RNA-RNA
      Interacting group
      Assay
      References
      p53 - mir-219
      luminiscence technology, fluorescence technology
      (Barrio et al., 2014)
      p53 - mir-279
      luminiscence technology
      (Barrio et al., 2014)
      p53 - mir-283
      luminiscence technology, fluorescence technology
      (Barrio et al., 2014)
      p53 - mir-305
      fluorescence technology, necessary binding region, luminiscence technology
      (Barrio et al., 2014)
      p53 - mir-1014
      luminiscence technology
      (Barrio et al., 2014)
      Alleles Reported to Model Human Disease (Disease Ontology) (40 alleles)
      Myc
      Models Based on Experimental Evidence ( 5 )
      Modifiers Based on Experimental Evidence ( 9 )
      Allele
      Disease
      Interaction
      References
      MycHMS01538
      exacerbates  carcinoma
      modeled by salrUAS.cBa
      (Sun et al., 2020)
      MycKK103869
      ameliorates  brain cancer
      modeled by bratKK113206
      (Rebelo and Homem, 2023)
      MycαTub84B.PBb
      model of  cancer
      is ameliorated by Pi3K92EUAS.Tag:MYC,Tag:PM(hKRAS)
      (Levayer et al., 2015)
      is ameliorated by shgUASp.cUa
      (Levayer et al., 2015)
      is ameliorated by diaUASp.EGFP
      (Levayer et al., 2015)
      is ameliorated by sqhRLC.N.GFP(S65T)
      (Levayer et al., 2015)
      is ameliorated by Diap1UAS.cHa
      (Levayer et al., 2015)
      MycUAS.Tag:HA
      exacerbates  carcinoma
      modeled by l(2)gl4
      (Sollazzo et al., 2022)
      MycUAS.cZa
      ameliorates  tuberous sclerosis
      modeled by Tsc1UAS.cTa, gigUAS.cTa
      (Tapon et al., 2001)
      ameliorates  Machado-Joseph disease
      modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
      (Singh et al., 2014)
      ameliorates  Huntington's disease
      modeled by Hsap\HTTQ138.UAS.EGFP,mRFP1
      (Singh et al., 2014)
      exacerbates  tauopathy
      modeled by Hsap\MAPTUAS.cWa
      (Pragati and Sarkar, 2021, Chanu and Sarkar, 2017)
      modeled by Hsap\MAPTR406W.UAS
      (Chanu and Sarkar, 2017)
      ameliorates  carcinoma
      modeled by salrUAS.cBa
      (Sun et al., 2020)
      modeled by Hsap\SALL4UAS.Tag:HA
      (Sun et al., 2020)
      model of  brain cancer
      is ameliorated by Spt5HMS00153
      (Hofstetter et al., 2024)
      MycGD1419
      ameliorates  cancer
      modeled by ykiUAS.cHa
      (Ziosi et al., 2010)
      ameliorates  high grade glioma
      modeled by Wdr62UAS.Tag:HA
      (Shohayeb et al., 2020)
      ameliorates  brain cancer
      modeled by bratHMS01121
      (Diaz et al., 2024)
      MycJF01762
      ameliorates  cystic kidney disease
      modeled by BicC5
      (Gamberi et al., 2017)
      exacerbates  Machado-Joseph disease
      modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
      (Singh et al., 2014)
      ameliorates  tauopathy
      modeled by Hsap\MAPTUAS.cWa
      (Chanu and Sarkar, 2017)
      modeled by Hsap\MAPTR406W.UAS
      (Chanu and Sarkar, 2017)
      ameliorates  frontotemporal dementia
      modeled by Hsap\MAPTV337M.UAS
      (Chanu and Sarkar, 2017)
      MycJF01761
      ameliorates  cystic kidney disease
      modeled by BicC5
      (Gamberi et al., 2017)
      ameliorates  tauopathy
      modeled by Hsap\MAPTV337M.UAS
      (Pragati et al., 2020)
      modeled by Hsap\MAPTUAS.cWa
      (Chanu and Sarkar, 2017, Chanu and Sarkar, 2017)
      modeled by Hsap\MAPTR406W.UAS
      (Chanu and Sarkar, 2017)
      ameliorates  frontotemporal dementia
      modeled by Hsap\MAPTV337M.UAS
      (Pragati et al., 2020, Chanu and Sarkar, 2017)
      exacerbates  tauopathy
      modeled by Hsap\MAPTUAS.cWa
      (Pragati and Sarkar, 2021)
      ameliorates  cancer
      modeled by HipkUAS.Tag:HA
      (Wong et al., 2020)
      MycRNAi.UAS.cUa
      ameliorates  high grade glioma
      modeled by aPKCUAS.Tag:CAAX(Unk)
      (Paglia et al., 2017)
      p53
      Models Based on Experimental Evidence ( 11 )
      Allele
      Disease
      Evidence
      References
      p53259H.GUS
      model of  cancer
      CEA
      (Sidorov and Belitskii, 2002)
      p535A-1-4
      model of  testicular cancer
      CEA
      (Napoletano et al., 2017)
      model of  cancer
      CEA
      (de la Cova et al., 2014)
      p53E8
      model of  testicular cancer
      CEA
      (Napoletano et al., 2017)
      p53H159N.UAS.Ex
      DOES NOT model  cancer
      CEA with pnutGD1512
      (Gerlach et al., 2018)
      p53GD4483
      model of  intestinal cancer
      CEA with ApcGD1333, Ras85DV12.cUa.UAS
      (Quintero and Bangi, 2023)
      CEA with ApcGD1333, PtenRNAi.UAS.cUa, Ras85DV12.cUa.UAS
      (Quintero and Bangi, 2023)
      CEA with ApcGD1333, MedGD1483, Ras85DV12.cUa.UAS
      (Quintero and Bangi, 2023)
      model of  cancer
      CEA with MycUAS.cZa
      (Diaz et al., 2024)
      p53GD11134
      model of  testicular cancer
      CEA
      (Napoletano et al., 2017)
      p53TM3
      model of  cancer
      CEA with p53-ns, Hsap\TP53R175H
      (D'Brot et al., 2017)
      CEA with p53-ns, Hsap\TP53G245S
      (D'Brot et al., 2017)
      CEA with p53-ns, Hsap\TP53R248Q
      (D'Brot et al., 2017)
      CEA with p53-ns, Hsap\TP53R273C
      (D'Brot et al., 2017)
      CEA with p53-ns, Hsap\TP53R273H
      (D'Brot et al., 2017)
      p53-ns
      model of  cancer
      CEA with p53TM3, Hsap\TP53R175H
      (D'Brot et al., 2017)
      CEA with p53TM3, Hsap\TP53G245S
      (D'Brot et al., 2017)
      CEA with p53TM3, Hsap\TP53R248Q
      (D'Brot et al., 2017)
      CEA with p53TM3, Hsap\TP53R273C
      (D'Brot et al., 2017)
      CEA with p53TM3, Hsap\TP53R273H
      (D'Brot et al., 2017)
      p53sgRNA.UAS
      model of  colorectal cancer
      CEA with Ras85DV12.cUa.UAS
      (Zipper et al., 2022)
      p53RNAi.UAS.RasG12D
      model of  pancreatic ductal adenocarcinoma
      CEA with CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.p53sh
      (Sekiya et al., 2023)
      CEA with CycEUAS.Tag:HA.Medsh, MedRNAi.UAS.CycE-HA, Ras85DG12D.UAS.cSa
      (Sekiya et al., 2023)
      model of  cancer
      CEA with MycUAS.cZa
      (Diaz et al., 2024)
      p53RNAi.UAS.cUa
      model of  colorectal cancer
      CEA with Ras85DV12.cUa.UAS, PtenGD13500, ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa
      (Bangi et al., 2016)
      CEA with Ras85DV12.cUa.UAS, PtenGD13500, ApcRNAi.UAS.cUa
      (Bangi et al., 2016)
      CEA with Ras85DV12.cUa.UAS, ApcRNAi.UAS.cUa, MedRNAi.UAS.cUa
      (Bangi et al., 2016)
      Modifiers Based on Experimental Evidence ( 15 )
      Allele
      Disease
      Interaction
      References
      p5311-1B-1
      ameliorates  Alexander disease
      modeled by Hsap\GFAPR79H.UAS
      (Wang et al., 2015)
      exacerbates  tauopathy
      modeled by Hsap\MAPTR406W.UAS
      (Merlo et al., 2014)
      ameliorates  brain ischemia
      modeled by Mmus\Gria1Lc.UAS
      (Lei et al., 2017)
      p53259H.GUS
      exacerbates  Alzheimer's disease 1
      modeled by Hsap\APPArctic.Aβ42.UAS.Tag:SS(rPENK)
      (Iijima et al., 2010)
      p535A-1-4
      ameliorates  Huntington's disease
      modeled by Hsap\HTTGMR.Q120
      (Bae et al., 2005)
      ameliorates  cytochrome-c oxidase deficiency disease
      modeled by ScoxGD898
      (Martínez-Morentin et al., 2015)
      exacerbates  tauopathy
      modeled by Hsap\MAPTR406W.UAS
      (Merlo et al., 2014)
      p53Ct.GUS
      exacerbates  Alzheimer's disease 1
      modeled by Hsap\APPArctic.Aβ42.UAS.Tag:SS(rPENK)
      (Iijima et al., 2010)
      p53GMR.Ex
      ameliorates  viral infectious disease
      modeled by EBV\BRLF1GMR.PA
      (Adamson and Lajeunesse, 2012)
      exacerbates  viral infectious disease
      modeled by EBV\BZLF1GMR.PA
      (Adamson and Lajeunesse, 2012)
      ameliorates  infectious mononucleosis
      modeled by EBV\BRLF1GMR.PA
      (Adamson and Lajeunesse, 2012)
      exacerbates  infectious mononucleosis
      modeled by EBV\BZLF1GMR.PA
      (Adamson and Lajeunesse, 2012)
      ameliorates  Epstein-Barr virus hepatitis
      modeled by EBV\BRLF1GMR.PA
      (Adamson and Lajeunesse, 2012)
      exacerbates  Epstein-Barr virus hepatitis
      modeled by EBV\BZLF1GMR.PA
      (Adamson and Lajeunesse, 2012)
      p53R155H.UAS.Ex
      ameliorates  ataxia telangiectasia
      modeled by tefuΔ356
      (Song et al., 2004)
      ameliorates  cancer
      modeled by Ptip3804, Ras85DV12.cUa.UAS
      (Dong et al., 2021)
      p53GL01032
      ameliorates  frontotemporal dementia and/or amyotrophic lateral sclerosis 1
      modeled by Zzzz\GGGGCC30.UAS.EGFP
      (Maor-Nof et al., 2021)
      p53GD11134
      model of  testicular cancer
      is NOT exacerbated by HtrA2DF1
      (Napoletano et al., 2017)
      p53KK113065
      ameliorates  cancer
      modeled by Ptip3804, Ras85DV12.cUa.UAS
      (Dong et al., 2021)
      p53JF02513
      ameliorates  Alexander disease
      modeled by Hsap\GFAPR79H.UAS
      (Wang et al., 2015)
      p53GL01220
      ameliorates  frontotemporal dementia and/or amyotrophic lateral sclerosis 1
      modeled by Zzzz\GGGGCC30.UAS.EGFP
      (Maor-Nof et al., 2021)
      exacerbates  amyotrophic lateral sclerosis type 1
      modeled by Hsap\SOD1A4V.UAS
      (Lu et al., 2019)
      exacerbates  frontotemporal dementia and/or amyotrophic lateral sclerosis 1
      modeled by Zzzz\poly-PRPO-100.UAS
      (Lu et al., 2019)
      p53UAS.cUa
      exacerbates  cytochrome-c oxidase deficiency disease
      modeled by ScoxGD898
      (Martínez-Morentin et al., 2015)
      exacerbates  brain ischemia
      modeled by Mmus\Gria1Lc.UAS
      (Lei et al., 2017)
      exacerbates  cancer
      modeled by Ras85DV12.cUa.UAS
      (Dong et al., 2021)
      p53HMS02286
      ameliorates  frontotemporal dementia and/or amyotrophic lateral sclerosis 1
      modeled by Zzzz\GGGGCC30.UAS.EGFP
      (Maor-Nof et al., 2021)
      p53DN.UAS.cUa
      ameliorates  cytochrome-c oxidase deficiency disease
      modeled by ScoxGD898
      (Martínez-Morentin et al., 2015)
      ameliorates  brain cancer
      modeled by dpnUAS.cUa
      (Morrissy et al., 2016)
      p53RNAi.UAS.cUa
      ameliorates  Alzheimer's disease 1
      modeled by Hsap\APPT12.Aβ42.UAS.Tag:SS(nec)
      (Taylor and Tuxworth, 2019)
      Hsap\MYC
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 3 )
      Hsap\TP53
      Models Based on Experimental Evidence ( 5 )
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Alleles Representing Disease-Implicated Variants
      Genetic Tools, Stocks and Reagents
      Sources of Stocks
      Contact lab of origin for a reagent not available from a public stock center.
      Bloomington Stock Center Disease Page
      Related mammalian, viral, bacterial, or synthetic transgenes
      Allele
      Transgene
      Publicly Available Stocks
      Hsap\TP53WT.1
      PBac{hTP53.WT1}
      Hsap\TP53WT.2
      PBac{hTP53.WT2}
      Hsap\TP53R175H
      PBac{hTP53.R175H}
      Hsap\TP53G245S
      PBac{hTP53.G245S}
      Hsap\TP53R248Q
      PBac{hTP53.R248Q}
      Hsap\TP53R273C
      PBac{hTP53.R273C}
      Hsap\TP53R273H
      PBac{hTP53.R273H}
      Hsap\TP53cH6FR.UAS
      P{UAS-Hsap\TP53.cH6FR}
      Hsap\TP53cH5FR.UAS
      P{UAS-Hsap\TP53.cH5FR}
      Hsap\TP53a.UAS
      P{UAS-Hsap\TP53.a}
      Hsap\TP53UAS.cYa
      P{UAS-Hsap\TP53.Y}
      Hsap\TP53C234.UAS
      P{UAS-Hsap\TP53.C234}
      Hsap\TP53UAS.cPa
      P{UAS-Hsap\TP53.P}
      Hsap\TP53UASp.Tag:HA
      P{UASp-hp53.HA}
      Hsap\MYCUAS.cUa
      P{UAS-hMYC.U}
      Hsap\MYChs.1
      P{hs-hMYC.1}
      Hsap\MYChs.2
      P{hs-hMYC.2}
      Hsap\MYCUAS.1
      P{UAS-hMYC.1}
      Hsap\MYCUAS.2
      P{UAS-hMYC.2}
      Hsap\MYCUAS.S
      P{UAS-hMYC.S}
      Selected Drosophila transgenes
      Allele
      Transgene
      Publicly Available Stocks
      p53sgRNA.UAS
      P{UAS-Apc,Apc2,p53,Med,Pten,cic.sgRNA}
      p53Ct.GUS
      P{GUS-p53.Ct}
      w1118; P{GUS-p53.Ct}3.1/TM6B, Tb1
      p53259H.GUS
      P{GUS-p53.259H}
      w1118; P{GUS-p53.259H}3.1
      p53R155H.UAS.Ex
      P{UAS-p53.R155H.Ex}
      y1 w1118; P{UAS-p53.R155H.Ex}2/T(2;3)TSTL, CyO: TM6B, Tb+
      p53H159N.UAS.Ex
      P{UAS-p53.H159N.Ex}
      y1 w1118; P{UAS-p53.H159N.Ex}2
      p53GMR.Ex
      P{GMR-p53.Ex}
      P{GMR-p53.Ex}1, y1 w1118/FM7c
      p53UAS.cUa
      P{UAS-p53.U}
      p53DN.UAS.cUa
      P{UAS-p535.DN.U}
      MycUAS.cZa
      P{UAS-Myc.Z}
      w1118; P{UAS-Myc.Z}132
      MycαTub84B.PBb
      P{alphaTub84B(FRT.Myc)lexA::GAD.A}
      P{ey-FLP.N}2, w* P{P-Sal}MycP0 P{αTub84B(FRT.Myc)GAL4.Bb}1/FM7a; rept35/MKRS
      MycUAS.Tag:HA
      P{UAS-Myc.HA}
      MycUAS.cUa
      P{UAS-Myc.U}
      RNAi constructs available
      Allele
      Transgene
      Publicly Available Stocks
      p53RNAi.sg
      PBac{sg-p53.RNAi}
      p53RNAi.UAS.RasG12D
      P{UAS-Ras85D(G12D).UAS-p53(shRNA)}
      p53RNAi.UAS.cUa
      P{UAS-p53.RNAi.U}
      p53GD4483
      P{GD4483}
      w1118; P{GD4483}v10692
      p53GD11134
      P{GD11134}
      w1118; P{GD11134}v45139
      p53KK113065
      P{KK113065}
      P{KK113065}VIE-260B
      p53RNAi.UAS
      P{UAS-p53.RNAi}
      p53JF02513
      P{TRiP.JF02513}
      y1 v1; P{TRiP.JF02513}attP2
      p53GL01032
      P{TRiP.GL01032}
      y1 sc* v1 sev21; P{TRiP.GL01032}attP2
      p53NIG.10873R
      P{NIG.10873R}
      P{NIG.10873R}1
      p53GL01220
      P{TRiP.GL01220}
      y1 v1; P{TRiP.GL01220}attP40
      p53HMS02286
      P{TRiP.HMS02286}
      y1 sc* v1 sev21; P{TRiP.HMS02286}attP2
      MycRNAi.UAS.cUa
      P{UAS-Myc.RNAi.U}
      MycHMJ22067
      P{TRiP.HMJ22067}
      y1 v1; P{TRiP.HMJ22067}attP40/CyO
      MycRNAi.13xlexAop.cZa
      P{13xlexAop-Myc.RNAi.Z}
      MycGD1419
      P{GD1419}
      w1118; P{GD1419}v2947
      MycJF01761
      P{TRiP.JF01761}
      y1 v1; P{TRiP.JF01761}attP2
      MycJF01762
      P{TRiP.JF01762}
      y1 v1; P{TRiP.JF01762}attP2
      MycKK103869
      P{KK103869}
      P{KK103869}VIE-260B
      MycHMS01538
      P{TRiP.HMS01538}
      y1 sc* v1 sev21; P{TRiP.HMS01538}attP2
      MycGL01314
      P{TRiP.GL01314}
      y1 sc* v1 sev21; P{TRiP.GL01314}attP40
      MycHMC03189
      P{TRiP.HMC03189}
      y1 v1; P{TRiP.HMC03189}attP40/CyO
      Selected Drosophila classical alleles
      Allele
      Allele class
      Mutagen
      Publicly Available Stocks
      p53TM3
      amorphic allele - genetic evidence
      p535A-1-4
      ends-in gene targeting
      y1 w1118; p535A-1-4
      p53E8
      amorphic allele - molecular evidence
      Delta2-3 transposase
      p53E4
      amorphic allele - molecular evidence
      Delta2-3 transposase
      p5311-1B-1
      ends-in gene targeting
      y1 w1118; p5311-1B-1
      p53-ns
      ends-in gene targeting
      w1118/Dp(1;Y)y+; TI{TI}p53-ns
      MycA51
      amorphic allele - molecular evidence
      P-element activity
      References (3)