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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000017403
Interaction Network
Interactions Browser links
Smn network
Gem2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0218395-2851.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 
unspecified role
Smn 
unspecified role
Smn 
unspecified role
Gem2 
unspecified role
Gem2 
unspecified role
Gem2 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 118.42722

FBrf0218721-1.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gem2 

Gemin2

neutral component

His tag

Gem2 

Gemin2

neutral component

His tag

Gem2 

Gemin2

neutral component

His tag

Smn 

SMNΔC

neutral component

His tag

Smn 
Smn 

SMNΔC

neutral component

His tag

Smn 

SMNΔC

neutral component

His tag

Smn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smn 
region
unspecified role
aa 1-122
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Proteins co-expressed in bacteria, purified by virtue of His tag, and the heterodimer purified by gel filtration.

Proteins expressed and purified from bacteria.

Smn and Gem2 form a heterodimer in vitro.

FBrf0218721-16.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMNΔC

neutral component
Smn 

SMNΔC

neutral component
Smn 

SMNΔC

neutral component
Gem2 

Gemin2

neutral component
Gem2 

Gemin2

neutral component
Gem2 

Gemin2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Components mixed and the complex purified by gel filtration.

Smn interacts with Gem2, SmD1, SmD2, SmE, SmF and SmG to form a 7S Sm core complex intermediate.

Gel filtration of the complex indicates a molecular mass consistent with a monomeric stoichiometry of each constituent.

Proteins expressed and purified from bacteria.

FBrf0221342-1.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMN

neutral component
Smn 

SMN

neutral component
Smn 
Smn 

SMN

neutral component
Smn 
Gem2 

Gemin2

neutral component
Gem2 

Gemin2

neutral component
Gem2 

Gemin2

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smn 
N-terminal region
sufficient binding region
aa 1-122
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified recombinant D. melanogaster proteins Gem2, Smn and icln were mixed with human SmD1, SmD2, SmE, SmF and SmG to reconstitute the 8S spliceosome assembly intermediate.

Electron microscopy 3D reconstruction was used to position those elements of the 8S complex that could not be traced in the crystallographic electron density.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

FBrf0226016-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

dSMN

bait

FLAG tag

Smn 
Gem2 

dGem2

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smn 
Gemin2 binding domain residue 20
mutation disrupting interaction
aa 20

D20V

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0235788-1134.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

Smn-RA

bait

fused to GAL4 DNA-binding domain

Gem2 

Gem2-RA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0235788-1134.Y2Hpool
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

Smn-RA

bait

fused to GAL4 DNA-binding domain

Gem2 

Gem2-RA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0235788-1150.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gem2 

Gem2-RA

bait

fused to GAL4 DNA-binding domain

Smn 

Smn-RA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0235788-1150.Y2Hpool
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gem2 

Gem2-RA

bait

fused to GAL4 DNA-binding domain

Smn 

Smn-RA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0237738-10.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gem2 

Gem2

bait

FLAG tag

Smn 

SMN

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0237738-13.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMN

bait

FLAG tag

Gem2 

Gem2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0245892-12.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

Smn

bait

GFP tag

Gem2 

Gem2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)