FB2022_02, released March 29, 2022

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
Spt5, spt4
FlyBase ID
FBig0000030155
Interaction Network
Interactions Browser links
Spt5 network
spt4 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222269-3.coIP.WB
Reference
Harvey et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Spt5 

Spt5

bait

FLAG tag

spt4 

Spt4

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Harvey et al., 2013)

The G994D mutation of Spt5W049 does not perturb the Spt5-Spt4 interaction.

(Harvey et al., 2013)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Harvey et al., 2013)
FBrf0227292-557.TFIN
Reference
Rhee et al., 2014
Description
physical association
Assay
Collection
TF_Interaction_Network-2
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
spt4 

FBgn0028683

unspecified role
spt4 

FBgn0028683

unspecified role
spt4 

FBgn0028683

unspecified role
Spt5 

FBgn0040273

unspecified role
Spt5 

FBgn0040273

unspecified role
Spt5 

FBgn0040273

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

(Rhee et al., 2014)

HGScore = 15.926869

(Rhee et al., 2014)
FBrf0235113-1.CH.MW
Reference
Qiu and Gilmour, 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spt5 

spt5

bait

FLAG tag

Spt5 
spt4 

spt4

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Spt5 
interaction domain
sufficient binding region
aa 1-634
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Qiu and Gilmour, 2017)
FBrf0237429-3.coIP.WB
Reference
Pereira and Paro, 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
Spt5 

Spt5

bait

FLAG tag

spt4 

Spt4

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pereira and Paro, 2017)

Control.

(Pereira and Paro, 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)