twin and Pop2 copurify in DEAE fractionation and subsequent gel filtration as part of the CCR4-NOT complex.

twin and Pop2 containing fractions contain poly(A)-specific 3\' exonuclease activity.

Source was cytoplasmic extract of S2 cell line; proteins produced from endogenous genes.

Cytoplasmic extract was first DEAE fractionated, then separated by gel filtration.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Catalytically inactive Pop2 is able to bind twin.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Catalytically inactive twin mutant proteins are able to bind Pop2.

Experiments were done in bam[Δ86] mutant ovaries, which contain only undifferentiated pre-cystoblasts.

Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The twin-Not1 interaction is not affected by RNase treatment.

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.

Hexameric CCR4-NOT-mini complex captured as 15 pairwise interactions.