FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000037490
Interaction Network
Interactions Browser links
Not1 network
Pop2 network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0211004-15.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not1 

Not1

bait
Pop2 

Caf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0211004-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not1 

Not1

bait
Pop2 

Caf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0218395-5050.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pop2 
unspecified role
Pop2 
unspecified role
Pop2 
unspecified role
Not1 
unspecified role
Not1 
unspecified role
Not1 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 165.37037

FBrf0220772-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pop2 

POP2

bait

GFP tag

Not1 

NOT1

prey

HA tag

Not1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Not1 
MIF4G
mutation disrupting interaction
aa 1277

K1277A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0220772-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not1 

NOT1

bait

GFP tag

Pop2 

POP2

prey

HA tag

Pop2 
Pop2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pop2 
region
mutation disrupting interaction
aa 151

E151A

Pop2 
region
mutation decreasing interaction
aa 155aa 162

S155A, weak effect on binding

E162A, weak effect on binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0221179-10.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pop2 

POP2

bait

GFP tag

Not1 

NOT1

prey

HA tag

Not1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Not1 
MIF4G domain
sufficient binding region
aa 1152-1376
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0221179-8.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not1 

NOT1

bait

GFP tag

Pop2 

POP2

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0257826-4.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not1 

Not1

neutral component
Not1 
Not1 

Not1

neutral component
Not1 
Not1 

Not1

neutral component
Pop2 

Caf1

neutral component
Pop2 

Caf1

neutral component
Pop2 

Caf1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Not1 
mini interaction domain
sufficient binding region
aa 1147-2505

N-terminally truncated; does not contain binding sites for entire CCR4-NOT complex.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.

Hexameric CCR4-NOT-mini complex captured as 15 pairwise interactions.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)