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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000053443
Interaction Network
Interactions Browser links
flw network
I-2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0122962-1.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

bait

fused to GAL4 DNA-binding domain

I-2 

I-2Dm

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0122962-2.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

enzyme

His tag

flw 

PP1β9C

enzyme

His tag

I-2 

I-2Dm

inhibitor

His tag

I-2 

I-2Dm

inhibitor

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0122962-3.CH
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

neutral component

His tag

flw 

PP1β9C

neutral component

His tag

flw 

PP1β9C

neutral component

His tag

I-2 

I-2Dm

neutral component

His tag

I-2 

I-2Dm

neutral component

His tag

I-2 

I-2Dm

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0194330-20.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 

PP1β9C

bait

fused to GAL4 DNA-binding domain

I-2 

Inhibitor-2

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from third instar larva cDNA expression library).

Two-hybrid system: yeast GAL4-BD/VP16-AD

FBrf0218395-6636.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flw 
unspecified role
flw 
unspecified role
flw 
unspecified role
I-2 
unspecified role
I-2 
unspecified role
I-2 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 241.45721

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)