Sin3A, Caf1 Interaction report

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General Information

Interaction Type

physical association

Interacting Genes

Sin3A, Caf1

FlyBase ID

FBig0000086172

Interaction Network

Interactions Browser links

Sin3A network

Caf1 network

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Reported Interactions

FBrf0211588-12.coIP.MS

Reference

(Spain et al., 2010)

Description

physical association

Assay

anti tag coimmunoprecipitation, peptide massfingerprinting

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Sin3A protein

SIN3

bait

HA tag

Caf1 protein

p55

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic extracts from transgenic lines carrying isoform-specific HA-tagged Sin3A constructs. Prey proteins were positively identified if minimally two unique peptides per protein scored above the 5% false discovery rate cut-off.

FBrf0211588-13.coIP.MS

Reference

(Spain et al., 2010)

Description

physical association

Assay

anti tag coimmunoprecipitation, peptide massfingerprinting

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Sin3A protein

SIN3

bait

HA tag

Caf1 protein

p55

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was S2 cell lines transfected with isoform-specific HA-tagged Sin3A plasmids. Prey proteins were positively identified if minimally two unique peptides per protein scored above the 5% false discovery rate cut-off.

FBrf0211588-20.coIP.WB

Reference

(Spain et al., 2010)

Description

physical association

Assay

anti tag coimmunoprecipitation, western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Sin3A protein

SIN3

bait

HA tag

Caf1 protein

p55

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was S2 cell lines transfected with isoform-specific HA-tagged Sin3A plasmids.

FBrf0211588-21.coIP.WB

Reference

(Spain et al., 2010)

Description

physical association

Assay

anti tag coimmunoprecipitation, western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Sin3A protein

SIN3

bait

HA tag

Caf1 protein

p55

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic extracts from transgenic lines carrying isoform-specific HA-tagged Sin3A constructs.

FBrf0211588-22.coIP.WB

Reference

(Spain et al., 2010)

Description

physical association

Assay

anti tag coimmunoprecipitation, western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Caf1 protein

p55

bait

FLAG tag

Sin3A protein

SIN3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

FBrf0211588-23.coIP.WB

Reference

(Spain et al., 2010)

Description

physical association

Assay

anti tag coimmunoprecipitation, western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Caf1 protein

p55

bait

FLAG tag

Sin3A protein

SIN3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryonic extract from a transgenic line carrying FLAG-tagged Caf1 construct.

References ( 0 )

No references are recorded in FlyBase at present.