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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000086228
Interaction Network
Interactions Browser links
hpo network
sav network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0161987-1.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait
hpo 
sav 

Sav

prey
sav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
C-terminal region
sufficient binding region
aa 318-669
sav 
C-terminal region
sufficient binding region
aa 362-607
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

6 out of 12 hpo-interacting clones corresponded to sav.

FBrf0161987-2.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait
sav 
hpo 

Hpo

prey
hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
C-terminal region
sufficient binding region
aa 474-669
sav 
C-terminal region
sufficient binding region
aa 319-607
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

5 out of 45 sav-interacting clones corresponded to hpo.

FBrf0161987-3.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

GST tag

hpo 

Hpo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

FBrf0161987-4.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

enzyme

Myc tag

hpo 

Hpo

enzyme

Myc tag

hpo 
sav 

Sav

enzyme target

GST tag, [32]P label

sav 

Sav

enzyme target

GST tag, [32]P label

sav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0161987-7.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

Myc tag

sav 

Sav

prey

FLAG tag

sav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
C-terminal region
necessary binding region
aa 529-607

\'shrp6\' mutant, ΔC-terminus

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0161988-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

MBP tag

sav 
hpo 

Hpo

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
N-terminal region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0161988-2.coIP.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

Myc tag

hpo 

Hpo

prey

[35]S label

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
C-terminal region
necessary binding region
aa 621-669

\'MGH3\' mutant, ΔC-terminus

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0161988-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

MBP tag

hpo 

Hpo

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0167603-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

HA tag

hpo 

dMST1

prey

FLAG tag

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
C-terminal region
sufficient binding region
aa 343-669

\'dMSTc\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0167603-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

dMST1

bait

FLAG tag

sav 

Sav

prey

HA tag

sav 
sav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
C-terminal region
sufficient binding region
aa 507-608

SavC2

sav 
coiled-coil domain
necessary binding region
aa 590-608

Sav\'ΔC1\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0167938-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

fused to GAL4 DNA-binding domain

hpo 

Hpo

prey

fused to GAL4 activation domain

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
C-terminal region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two out of 108 sav-interacting clones corresponded to hpo.

FBrf0167938-2.coIP.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

Myc tag, [35]S label

sav 
hpo 

Hpo

prey

[35]S label

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
dimerization domain
sufficient binding region
aa 590-669
sav 
Sav-specific domain
sufficient binding region
aa 491-609
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

FBrf0167939-1.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

fused to LexA DNA-binding domain

hpo 

Hippo

prey

fused to B42 activation domain

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
C-terminal region
sufficient binding region
aa 602-669
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

FBrf0167939-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

fused to LexA DNA-binding domain

sav 
hpo 

Hippo

prey

fused to B42 activation domain

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
C-terminal region
sufficient binding region
aa 311-608
hpo 
C-terminal region
sufficient binding region
aa 602-669
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0167939-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

FLAG tag

sav 

SAV

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0192943-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

HA tag

hpo 

Hpo

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0192943-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

FLAG tag

sav 

Sav

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0192943-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait
hpo 

Hpo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0210017-18.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

fused to GAL4 DNA-binding domain

hpo 

Hpo

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0211636-1.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

FLAG tag

sav 

Sav

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0211636-2.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

FLAG tag

hpo 

Hpo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

sav protein as bait failed to recover any of the other members of the Rassf/hpo complex, supporting a previous proposal that the sav/hpo complex is a separate entity.

FBrf0216584-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

FLAG tag

sav 

Sav

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The C-terminal SARAH domain of sav is not required for its interaction with hpo.

Bait expressed in transfected cells.

Prey expressed in transfected cells.

FBrf0217990-4.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

Myc tag

sav 
hpo 

Hpo

prey

FLAG tag

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
SARAH domain
mutation disrupting interaction
aa 634

I634D

sav 
SARAH domain
mutation disrupting interaction
aa 563

L563P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Mutation of each SARAH domain in hpo and sav disrupts the interaction.

FBrf0217990-5.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

FLAG tag

hpo 
sav 

Sav

prey

Myc tag

sav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpo 
SARAH domain
mutation disrupting interaction
aa 634

I634D

sav 
SARAH domain
mutation disrupting interaction
aa 563

L563P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Mutation of each SARAH domain in hpo and sav disrupts the interaction.

FBrf0220751-8.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

FLAG tag

hpo 

Hpo

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

Cotransfection of Sik2 does not affect the hpo-sav interaction.

FBrf0222342-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

Myc tag

hpo 

Hpo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

par-1 decreases the hpo-sav interaction in a kinase dependent manner.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0222652-4.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

FLAG tag

hpo 

Hpo

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0224345-104.coIP.MS
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

hpo

bait

Protein A tag, Strep tag

sav 

sav

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0224345-148.coIP.MS
Description
physical association
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

sav

bait

Protein A tag, Strep tag

hpo 

hpo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0227228-2.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hippo

bait

TAP tag

sav 

Sav

prey

2 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0229736-4.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait
hpo 

Hpo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0229775-12.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hpo

bait

Myc tag

sav 

Sav

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Increased expression of ci has no effect on binding between hpo and sav.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0231779-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

sav

bait

Myc or FLAG tag

sav 
hpo 

hpo

prey

FLAG or Myc tag

hpo 
hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
interaction domain
sufficient binding region
aa 294-608

coordinates relative to sav-PA

hpo 
interaction domain
sufficient binding region
aa 1-596
hpo 
interaction domain residue 591
mutation disrupting interaction
aa 591

Y591G

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0238596-1.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Salvador

neutral component

His, SUMO tag

sav 

Salvador

neutral component

His, SUMO tag

sav 
sav 

Salvador

neutral component

His, SUMO tag

sav 
hpo 

Hippo

neutral component

His, SBP tag

hpo 
hpo 

Hippo

neutral component

His, SBP tag

hpo 

Hippo

neutral component

His, SBP tag

hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
SARAH domain
sufficient binding region
aa 531-608

coordinates relative to sav-PA

hpo 
SARAH domain
sufficient binding region
aa 606-662
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

FBrf0238596-2.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Salvador

neutral component
sav 

Salvador

neutral component
sav 
sav 

Salvador

neutral component
sav 
hpo 

Hippo

neutral component
hpo 
hpo 

Hippo

neutral component
hpo 

Hippo

neutral component
hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
SARAH domain
sufficient binding region
aa 531-608

coordinates relative to sav-PA

hpo 
SARAH domain
sufficient binding region
aa 606-662
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

FBrf0238596-3.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpo 

Hippo

bait
hpo 
sav 

Salvador

prey
sav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
SARAH domain
sufficient binding region
aa 531-608

coordinates relative to sav-PA

hpo 
SARAH domain
sufficient binding region
aa 606-662
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The longer SARAH domain of sav, aa 530-608, binds the hpo SARAH domain more strongly than a shorter sav fragment, aa 552-608.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0238596-4.CL.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Salvador

neutral component
sav 

Salvador

neutral component
sav 
sav 

Salvador

neutral component
sav 
hpo 

Hippo

neutral component
hpo 
hpo 

Hippo

neutral component
hpo 

Hippo

neutral component
hpo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sav 
SARAH domain
sufficient binding region
aa 531-608

coordinates relative to sav-PA

hpo 
SARAH domain
sufficient binding region
aa 606-662
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

FBrf0244980-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

FLAG tag

hpo 

Hpo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Co-expression of AsnRS diminishes the sav-hpo interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (20)