Two-hybrid system: yeast GAL4-BD/GAL4-AD.
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
Positive control.
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
Positive control.
C109,114S, coordinates relative to CkIIβ-PB
Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
C109S,C114S, coordinates relative to CkIIβ-PB
C137S,C140S, coordinates relative to CkIIβ-PB
Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.
M161K,E165D,deletion of aa 234-240,R242E
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD. The CkIIα Tik mutation, a unique dominant mutation, consists of two aa substitutions- M161K and E165D. TikR, a partial phenotypic revertant of Tik consists of M161K,E165D,deletion of aa 234-240 and R242E. The Tik form of CkIIα still binds CkIIβ while TikR does not.
HGScore = 418.46030
Tagged transgenic construct was expressed in tim[+] cells.
Positive control.
Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Tagged transgenic construct was expressed in tim[+] cells.
Positive control.
Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)
MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)
MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
